Stock Number: 006906 Strain Name: B6.Cg-Lepob Ldlrtm1Her/J

Allele: Lepob Protocol Name: Lepob Method: Pyrosequencing Version: 2.1 Created: 23-September-2008 Updated: 24-September-2009

Notes: This genotyping assay uses pyrosequencing technology and is run on the Biotage PSQ 96MA. The Jackson Laboratory is not posting the complete details of our pyrosequencing genotyping assays as the primers for pyrosequencing cannot be used for sequencing using more traditional methods. The wild type and mutant nucleotides and the flanking DNA sequence are provided below. Expected Results: Mutant = T/T Heterozygote = C/T Wild type = C/C

Sequence: GCTCCGTGGCTCCCAGTCAGCTGATGACAGGAAGTAAGGGCCTGGACCAGGAAGGTG AGAAGGAAGGAGGTAGCCCAGGTTCACAGATGTAATGAAGGGCTCTGGAGACCGATC TCCCTGCCACTTGCTAAAGCACCTCTTGTTCTTCTTCCTCCTGCATAGCAGTCGGTATCC GCCAAGCAGAGGGTCACTGGCTTGGACTTCATTCCTGGGCTTCACCCCATTCTGAGTTT GTCCAAGATGGACCAGACTCTGGCAGTCTATCAACAGGTCCTCACCAGCCTGCCTTCCC AAAATGTGCTGCAGATAGCCAATGACCTGGAGAATCTC(c/t)GAGACCTCCTCCATCTGCT GGCCTTCTCCAAGAGCTGCTCCCTGCCTCAGACCAGTGGCCTGCAGAAGCCAGAGAGC CTGGATGGCGTCCTGGAAGCCTCACTCTACTCCACAGAGGTGGTGGCTTTGAGCAGGCT GCAGGGCTCTCTGCAGGACATTCTTCAACAGTTGGATGTTAGCCCTGAATGCTGAAGTTT CAAAGGCCACCAGGCTCCCAAGAATCATGTAGAGGGAAGAAACCTTGGCTTCCAGGGG TCTTCAGGAGAAGAGAGCCATGTGCACACATCCATCATTCATTTCTCTCCCTCCTGTAGA CCACCCATCCAAAGGCATGACTCCACAATGCTTGACTCAAGTTATCCACACAACTTCAT GAGCACAAGGAGGGGC

This genotyping protocol is used for the following strains:   Stock Number Strain Name 000632 B6.V-Lepob/J 000696 BKS.V-Lepob/J 004824 BTBR.V(B6)-Lepob/WiscJ 006906 B6.Cg-Lepob Ldlrtm1Her/J Genotyping resources and troubleshooting This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.