Stock Number:017484
Strain Name:FVB-Tg(JPH3-GFP,-JPH3*)GXwy/J
Generic GFP
Fluorescent Proteins (Generic GFP)

Robotic MCA

4.0
28-May -2010 21-August -2012
Notes: Melting curve analysis is done using a Roche Light Cycler 480.

This assay will NOT distinguish hemizygous from homozygous transgenic animals.
Expected Results: Transgene Tm = 89.5°C +/- 1.0°C
Internal positive control Tm = 79.4°C +/- 1.0°C
Gel Image 
Image Image file
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Protocol Primers 
Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type
oIMR0872 - AAG TTC ATC TGC ACC ACC G - Transgene
oIMR3999 - TGC TCA GGT AGT GGT TGT CG - Transgene
oIMR7338 - CTA GGC CAC AGA ATT GAA AGA TCT - Internal Positive Control Forward
oIMR7339 - GTA GGT GGA AAT TCT AGC ATC ATC C - Internal Positive Control Reverse
Reaction/Components 
Reaction Component Volume (µl) Final
Concentration		 Total Volume (µl)
ddH2O 3.87 - 3.87
2 X SYBR Green Master Mix (Roche) 6.00 1.00 X 6.00
DNA 2.00 - 2.00
92 X PM Generic GFP 0.13 1.00 X 0.13
Cycling 
Step # Temp °C Time Note
1 94 5 RS 68-1
2 94 15 sec -
3 68 1 min -
4 72 30 sec repeat steps 2-4 for 35 cycles
5 72 2 min -
6 10 - hold
This genotyping protocol is used for the following strains:
 
Stock Number Strain Name
003116 STOCK Tg(CAG-EGFP)D4Nagy/J
003718 FVB-Tg(GadGFP)45704Swn/J
004353 C57BL/6-Tg(UBC-GFP)30Scha/J
005854 STOCK Tg(Cp-EGFP)25Gaia/J
006000 B6.FVB-Tg(ITGAM-DTR/EGFP)34Lan/J
007484 B6.Cg-Tyrc-2J Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ
007860 C57BL/6J-Tg(Eno2-YFP/Cox8a)ZRwb/J
007977 B6;CBA-Tg(Hf/Olfr16-GFP)7Mom/MomJ
007978 B6;CBA-Tg(Hf/Olfr16-GFP)47Mom/MomJ
007979 B6;CBA-Tg(H/Olfr16-GFP)3Mom/MomJ
007980 B6;CBA-Tg(H/Olfr16-GFP)4Mom/MomJ
007981 B6;CBA-Tg(H/Olfr16-GFP)6Mom/MomJ
007982 B6;CBA-Tg(H/Olfr16-taumRFP,-tauGFP)8Mom/MomJ
007983 B6;CBA-Tg(H/Olfr16-taumRFP,-tauGFP)9Mom/MomJ
007984 B6;CBA-Tg(H/Olfr16-taumCherry,-tauGFP)11Mom/MomJ
007985 B6;CBA-Tg(H/Olfr16-taumCherry,-tauGFP)13Mom/MomJ
007986 B6;CBA-Tg(H*/Olfr16-GFP)11Mom/MomJ
007987 B6;CBA-Tg(H*/Olfr16-GFP)25Mom/MomJ
012350 B6;SJL-Tg(Thy1-COP4*H134R/EYFP)20Gfng/J
015808 B6.Cg-Tg(UBC-TVA)1Clc/J
016925 129;B6-Grin3b/Tmem259tm1Zhang Tg(Prnp-C19ORF6,-GFP)6Zhang/J
017484 FVB-Tg(JPH3-GFP,-JPH3*)GXwy/J
017494 B6;D-Tg(Tshz3-GFP/cre)43Amc/J
017592 B6;129S-Sox2tm2Hoch/J
018548 FVB/N-Tg(GFAP-Cadm1/EGFP)42Oje/J
Genotyping resources and troubleshooting
This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.