Stock Number: 006665 Strain Name: B6;129P2-Olfr151tm13(rI7)Mom/MomJ

Allele: Generic LacZ Protocol Name: Generic LacZ Melt Curve Analysis Method: Melt Curve Analysis Version: 2.1 Created: 18-September-2008 Updated: 24-September-2009

Expected Results: Transgene Tm = 87°C +/- 1.0°C Internal positive control Tm = 82.1°C+/- 1.0°C

Gel Image

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Protocol Primers

Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type oIMR0040 - CGT GGC CTG ATT CAT TCC - Transgene Reverse oIMR3054 - ATC CTC TGC ATG GTC AGG TC - Transgene Forward oIMR8744 - CAA ATG TTG CTT GTC TGG TG - Internal Positive Control Forward oIMR8745 - GTC AGT CGA GTG CAC AGT TT - Internal Positive Control Reverse

Number of Reactions

Reaction/Components  Reaction Component Volume (µl) Final Concentration Total Volume (µl) ddH2O 2.80 - 2.80 2 X SYBR Green Master Mix (Roche) 6.00 1.00 X 6.00 20 uM oIMR3054 0.30 0.50 uM 0.30 20 uM oIMR0040 0.30 0.50 uM 0.30 20 oIMR8744 0.30 0.50 0.30 20 uM oIMR8745 0.30 0.50 uM 0.30 DNA 2.00 - 2.00

Cycling  Step # Temp °C Time Note 1 94 5 min - 2 94 30 sec - 3 50 45 sec - 4 72 30 sec repeat steps 2-4 for 12 cycles 5 72 2 min - 6 10 - hold

This genotyping protocol is used for the following strains:   Stock Number Strain Name 002621 B6;SJL-Tg(tetop-lacZ)2Mam/J 002856 FVB/N-Tg(TIE2-lacZ)182Sato/J 003299 B6;SWJ-Tg(TIMP3-lacZ)7Jeb/J 004141 B6;CBA-Tg(UAS-lacZ)65Rth/J 004623 STOCK Tg(Fos-lacZ)34Efu/J 005317 B6.Cg-Tg(BAT-lacZ)3Picc/J 005493 STOCK Tg(Tek-rtTA,TRE-lacZ)1425Tpr/J 005728 STOCK Tg(tetO-Ipf1,lacZ)958.1Macd/J 005814 B6.129P2-Grm1tm1Dgen/J 006229 B6.Cg-Tg(DRE-lacZ)2Gswz/J 006465 B6;CBA-Tg(CAG-lacZ-WGA)330Bbm/J 006477 B6.Cg-Tg(CAG-lacZ-WGA)330Bbm/J 006578 STOCK Myoz2tm1Eno/J 006613 STOCK Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe/J 006665 B6;129P2-Olfr151tm13(rI7)Mom/MomJ 006672 B6;CBA-Tg(Olfr16*,taulacZ)7Mom/MomJ 006686 STOCK Olfr6tm1Mom/MomJ 008344 B6;DBA-Tg(Fos-tTA,Fos-EGFP*)1Mmay Tg(tetO-lacZ,tTA*)1Mmay/J 008477 STOCK Tg(RARE-Hspa1b/lacZ)12Jrt/J 008704 B6;129-Smn1tm6(SMN2)Mrph/J 008875 B6.129P2-Lgr5tm1(cre/ESR1)Cle/J 904497 R26_LSL_Z2S Genotyping resources and troubleshooting This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.