Stock Number:005252
Strain Name:B6EiC3Sn.BLiA-Ts(1716)65Dn/DnJ
Ts(1716)65Dn
Ts1716
High Resolution Melting
1.0
09-March -2011 17-February -2012
Expected Results:

use Evagreen
Gel Image 
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Protocol Primers 
Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type
11981 GTG GCA AGA GAC TCA AAT TCA AC Mutant
11982 TGG CTT ATT ATT ATC AGG GCA TTT Mutant
oIMR7338 - CTA GGC CAC AGA ATT GAA AGA TCT - Internal Positive Control
oIMR7339 - GTA GGT GGA AAT TCT AGC ATC ATC C - Internal Positive Control
Reaction/Components 
Reaction Component Volume (µl) Final
Concentration		 Total Volume (µl)
ddH2O 1.74 - 1.74
2 X High Resolution Melt Master Mix (Roche) 6.00 1.00 X 6.00
25 mM MgCl2 0.96 2.00 mM 0.96
DNA 2.00 - 2.00
20 uM oIMR7338 0.05 0.08 uM 0.05
20 uM 11981 0.60 1.00 uM 0.60
20 uM 11982 0.60 1.00 uM 0.60
20 uM oIMR7339 0.05 0.08 uM 0.05
Cycling 
Step # Temp °C Time Note
1 94 10 min -
2 94 30 sec -
3 59 1 min -
4 72 45 sec repeat steps 2-4 for 35 cycles
5 72 2 min -
6 10 - hold
This genotyping protocol is used for the following strains:
 
Stock Number Strain Name
001924 B6EiC3Sn a/A-Ts(1716)65Dn/J
004850 B6EiC3Sn-Rb(12.Ts171665Dn)2Cje/CjeDnJ
005252 B6EiC3Sn.BLiA-Ts(1716)65Dn/DnJ
Genotyping resources and troubleshooting
This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.