Stock Number:003780
Strain Name:B6.Cg-Sgshmps3a/PstJ
Sgshmps3a
Sgshmps3a End Point
End Point Analysis
1.0
09-February -2012 25-February -2012
Expected Results:

Mutant = A
Wild type = G
Sequence:

TACACACACACACACACACACACACACACATACCCCTCAGCTGTATCCTAGGCGTGTCTCTCCTGCCTGGCTGTCACTTGTGAGGTAGAGTCTTCAGAGCCCCAGTAGTCTGCAGGGATTACATGCAGCCATCACCTACTAACACACCTGCATGGACTCCAGTCTGTTGGGGTGTGAGCTCCTCACGCTAGCCTACCTGTTAACATTCCCACCTGAGTTTCCTGTGTTCCCTGCAAACCATGGTGTCTTCTCCCCCTGCTAACATATCACTCAGGGATCTTGCCTACCTGTGTTCCCTGCCTGCTCACAAACTCCTATATCGTTGTGCCTGGCATCACACCCCCTCAGGCTTTCCTTCTTCTGGGAGTGTCTAGTCCAACTCCTGAGCATCACAGGGACCAGCCTTTGCAGCAGTACCCTAGGTATCCCCCAGCCCCCACTGTCCCTCCTGACCCCAGCCAACACGCCCTCTGCTCTGTCTTCCTCAGCG[g/a]ATGACGGAGGCTTTGAGAGTGGTGTATACAACAACACTGCCATCGCCACCCCTCACCTGGATGCCCTGTCCCGCCACAGCCTTATCTTCCGTAACGCCTTCACGTCTGTCAGCAGCTGTTCCCCGAGCCGTGCCAGCCTCCTCACCGGCCTGCCCCAGGTGAGAGTGGCTAGTGGGATGAGGGGACTGGGCGGGTCGGGGGGTGGATAGGTGGTGACTCTAGGGTCCTCTCTCCTGGGAGGTGCTACCTGGTTTCCCTTGCATCCTTAGGGGCAGGTACTCCCCAGAGGTCTCAGGTGCAGCCAGGGCTCTGCCTCCACACCCCTCAGTTCAGCTGGCTCAGTGACTAAGTGTCCTCATCTGAGACGTGGGCACAGTGAGGTGTCACTTGAAGGTCATTGTGGGTGTTGGTCAAGCACGGCTTTGCGGGGAGAAAGTGCTCAGCATAGACAGAACCTGCACACCTCCCGCCCAGCCCTGAGCTCAGAACCTCCGTGAGGTCAGGCACTTCCTTTTTCTGTTGTTGTGGTAAAGTACATAAAACATAAAATTTGCCATCT
Protocol Primers 
Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type
14064 6-FAM TCC TCA GCG AAT GAC GGA

Iowa Black

 Probe
14065 JOE NHS Ester TCC TCA GCG GAT GAC GGA

Iowa Black

 Probe
9883 Biotin ACC AGC CTT TGC AGC AGT A - Forward
9884 - GAA CAG CTG CTG ACA GAC GT - Reverse
Reaction/Components 
Reaction Component Volume (µl) Final
Concentration		 Total Volume (µl)
2 X Kapa Probe Fast QPCR 2.50 1.00 X 2.50
ddH2O 0.07 - 0.07
10 ul 9883 0.02 0.04 ul 0.02
10 ul 9884 0.02 0.04 ul 0.02
100 uM 14064 0.01 0.20 uM 0.01
100 uM 14065 0.01 0.20 uM 0.01
DNA 2.37 - 2.37
Cycling 
Step # Temp °C Time Note
1 95 10min -
2 92 15sec -
3 60 1 min -
4 - - repeat steps 2-3 for 40 cycles
5 4 - Forever
This genotyping protocol is used for the following strains:
 
Stock Number Strain Name
003780 B6.Cg-Sgshmps3a/PstJ
Genotyping resources and troubleshooting
This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.