Stock Number:007985
Strain Name:B6;CBA-Tg(H/Olfr16-taumCherry,-tauGFP)13Mom/MomJ
Generic GFP
Fluorescent Proteins -- Generic GFP
QPCR
11.0
20-December -2011 18-October -2012
Notes: Taqman qPCR protocols are run on an ABI 7500, 7700, 7900 or the Roche LightCycler
480. Use an appropriate instrument specific Fluorophore/Quencher combination.
The transgene genotype is determined by comparing ΔCt values of each unknown
sample against known homozygous and hemizygous controls, using appropriate
endogenous references.
Protocol Primers 
Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type
13731 6-FAM TTC AAG TCC GCC ATG CCC GAA

Zen probe with Iowa Black

 Tg Probe
13762 JOE NHS Ester CCA ATG GTC GGG CAC TGC TCA A

Zen probe with Iowa Black

 IC Probe
oIMR1544 - CAC GTG GGC TCC AGC ATT - Internal Positive Control Forward
oIMR1856 - CCA CAT GAA GCA GCA GGA CTT - Transgene Forward
oIMR1857 - GGT GCG CTC CTG GAC GTA - Transgene Reverse
oIMR3580 - TCA CCA GTC ATT TCT GCC TTT G - Internal Positive Control Reverse
Reaction/Components 
Reaction Component Volume (µl) Final
Concentration		 Total Volume (µl)
2 X PerfeCTa qPCR SuperMix 6.25 1.00 X 6.25
40 uM oIMR1856 0.13 0.42 uM 0.13
20 uM oIMR1857 0.13 0.21 uM 0.13
40 uM oIMR1544 0.13 0.42 uM 0.13
40 uM oIMR3580 0.13 0.42 uM 0.13
DNA 5.00 - 5.00
ddH2O 0.00 - 0.00
5 uM 13731 0.38 0.15 uM 0.38
5 uM 13762 0.38 0.15 uM 0.38
Cycling 
Step # Temp °C Time Note
1 50 2 min -
2 95 10 min -
3 95 15 sec -
4 60 1 min repeat steps 3-4 for 40 cycles
This genotyping protocol is used for the following strains:
 
Stock Number Strain Name
003516 FVB.Cg-Tg(CAG-EGFP)B5Nagy/J
003718 FVB-Tg(GadGFP)45704Swn/J
004218 B6.129(ICR)-Tg(CAG-ECFP)CK6Nagy/J
004353 C57BL/6-Tg(UBC-GFP)30Scha/J
004509 B6.FVB-Tg(Itgax-DTR/EGFP)57Lan/J
004512 C.FVB-Tg(Itgax-DTR/EGFP)57Lan/J
004654 B6;CBA-Tg(Pou5f1-EGFP)2Mnn/J
005070 C57BL/6-Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck/J
005244 B6.Cg-Tg(Krt1-15-EGFP)2Cot/J
005334 NOD/ShiLt-Tg(Cd4-EGFP)1Lt/J
005483 129-Tg(CAG-EYFP)7AC5Nagy/J
005515 FVB-Tg(ITGAM-DTR/EGFP)34Lan/J
005627 B6.Cg-Tg(Thy1-YFP/Syp)10Jrs/J
005630 B6.Cg-Tg(Thy1-EYFP)15Jrs/J
005738 B6.FVB-Tg(tetO-EGFP,-Tgfbr2)8Mcle/J
005854 STOCK Tg(Cp-EGFP)25Gaia/J
006000 B6.FVB-Tg(ITGAM-DTR/EGFP)34Lan/J
006214 FVB.Cg-Smn1tm1Msd/J
006567 C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ
006864 B6.Cg-Tg(Ins1-EGFP)1Hara/J
007612 B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J
007615 B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J
007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J
007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J
007977 B6;CBA-Tg(Hf/Olfr16-GFP)7Mom/MomJ
007979 B6;CBA-Tg(H/Olfr16-GFP)3Mom/MomJ
007980 B6;CBA-Tg(H/Olfr16-GFP)4Mom/MomJ
007983 B6;CBA-Tg(H/Olfr16-taumRFP,-tauGFP)9Mom/MomJ
007984 B6;CBA-Tg(H/Olfr16-taumCherry,-tauGFP)11Mom/MomJ
007985 B6;CBA-Tg(H/Olfr16-taumCherry,-tauGFP)13Mom/MomJ
007986 B6;CBA-Tg(H*/Olfr16-GFP)11Mom/MomJ
007987 B6;CBA-Tg(H*/Olfr16-GFP)25Mom/MomJ
008173 NOD.Cg-Tg(Ins1-EGFP)1Hara/QtngJ
008450 FVB-Tg(CAG-luc,-GFP)L2G85Chco/J
008547 NOD.FVB-Tg(ITGAM-DTR/EGFP)34Lan/JdkJ
008549 NOD.FVB-Tg(Itgax-DTR/EGFP)57Lan/JdkJ
008605 B6;C3-Tg(CAG-DsRed,-EGFP)5Gae/J
009354 FVB/N-Tg(Dazl-EGFP)10Rarp/J
009422 NOD.Cg-Tg(Itgax-Venus)1Mnz/QtngJ
011107 STOCK Tg(CAG-Venus)1Hadj/J
012350 B6;SJL-Tg(Thy1-COP4*H134R/EYFP)20Gfng/J
012370 FVB/NJ-Tg(Hspa1a-luc,-EGFP)2Chco/J
013751 STOCK Iis2tm2(ACTB-tdTomato,-EGFP)Luo/J
014135 B6.Cg-Tg(Fos/EGFP)1-3Brth/J
014548 B6.Cg-Tg(Slc32a1-COP4*H134R/EYFP)8Gfng/J
014555 B6;SJL-Tg(Tph2-COP4*H134R/EYFP)5Gfng/J
016925 129;B6-Grin3b/Tmem259tm1Zhang Tg(Prnp-C19ORF6,-GFP)6Zhang/J
Genotyping resources and troubleshooting
This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.