Stock Number: 002959 Strain Name: B6.129S4-Drd1atm1Jcd/J

Allele: Drd1atm1Jcd Protocol Name: Drd1atm1Jcd Method: Standard PCR Version: 3.0 Created: 31-July -2009 Updated: 23-November -2009

Gel Image

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Gel Information: Separated by gel electrophoresis on a 1.5% agarose gel.

Protocol Primers

Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type 9448 - CCT CCC TGA ACC CCA TTA TT - Wild type Forward 9449 - AGC AGA GGG GTC TCG TGT TA - Common oIMR8444 - GCC TGA AGA ACG AGA TCA GC - Mutant Forward

Number of Reactions

Reaction/Components  Reaction Component Volume (µl) Final Concentration Total Volume (µl) ddH2O 3.36 - 3.36 25 mM MgCl2 0.96 2.00 mM 0.96 2.5 mM dNTP 0.96 0.20 mM 0.96 5 mM DNA Loading Dye 1.66 0.69 mM 1.66 DNA 2.00 0.00 2.00 10 X AB PCR BufferII 1.20 1.00 X 1.20 5 U/ul Taq DNA Polymerase 0.06 0.03 U/ul 0.06 20 uM 9448 0.60 1.00 uM 0.60 20 uM 9449 0.60 1.00 uM 0.60 20 uM oIMR8444 0.60 1.00 uM 0.60

Cycling  Step # Temp °C Time Note 1 94 3 min - 2 94 30 sec - 3 61 1 min - 4 72 1 min - 5 - - Step 2-35 cycles 6 72 2 min - 9 10 0 Forever

This genotyping protocol is used for the following strains:   Stock Number Strain Name 002322 STOCK Drd1atm1Jcd/J 002959 B6.129S4-Drd1atm1Jcd/J Genotyping resources and troubleshooting This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.