Stock Number:017729
Strain Name:B6.129-Aurkatm1.1Tvd/J
Aurkatm1.1Tvd
Aurkatm1.1Tvd
Melt Curve Analysis
2.0
26-April -2012 26-April -2012
Expected Results:

Mutant = 625 bp
Heterozygote = 498 bp and 625 bp
Wild type = 498 bp
Gel Image 
Protocol Primers 
Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type
13993 - TGA GTT GGA AAG GGA CAT GG - Forward
13994 - CTC CAC CAC CTT ACT GTT GG - Reverse
Reaction/Components 
Reaction Component Volume (µl) Final
Concentration		 Total Volume (µl)
ddH2O 3.95 - 3.95
5 X Kapa 2G HS buffer 2.40 1.00 X 2.40
25 mM MgCl2 0.96 2.00 mM 0.96
10 mM dNTP KAPA 0.24 0.20 mM 0.24
20 uM 13993 0.30 0.50 uM 0.30
20 uM 13994 0.30 0.50 uM 0.30
5 mM 10x Loading Dye 1.20 0.50 mM 1.20
2.5 ul Kapa 2G HS taq polymerase 0.05 0.01 ul 0.05
20 X EvaGreen 0.60 1.00 X 0.60
DNA 2.00 - 2.00
Cycling 
Step # Temp °C Time Note
1 94 2 min -
2 94 20sec -
3 65 15sec -1.5 C per cycle
4 68 10sec -
5 - - repeat steps 2-4 for 10 cycles
6 94 15sec -
7 50 15sec -
8 72 10sec -
9 - - repeat steps 6-8 for 28 cycles
10 72 2 min -
11 10 - hold
This genotyping protocol is used for the following strains:
 
Stock Number Strain Name
017729 B6.129-Aurkatm1.1Tvd/J
Genotyping resources and troubleshooting
This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.