Stock Number:005728
Strain Name:STOCK Tg(tetO-Ipf1,lacZ)958.1Macd/J

Generic LacZ
Generic LacZ Melt Curve Analysis
Melt Curve Analysis
3.0
05-January -2010 06-March -2013
Expected Results: Transgene Tm = 87°C +/- 1.0°C
Internal positive control Tm = 82.1°C+/- 1.0°C
Gel Image 
Image Image file
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Protocol Primers 
Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type
oIMR0040 - CGT GGC CTG ATT CAT TCC - Transgene Reverse
oIMR3054 - ATC CTC TGC ATG GTC AGG TC - Transgene Forward
oIMR8744 - CAA ATG TTG CTT GTC TGG TG - Internal Positive Control Forward
oIMR8745 - GTC AGT CGA GTG CAC AGT TT - Internal Positive Control Reverse
Reaction/Components 
Reaction Component Volume (µl) Final
Concentration
Total Volume (µl)
ddH2O 2.80 - 2.80
2 X SYBR Green Master Mix (Roche) 6.00 1.00 X 6.00
20 uM oIMR3054 0.30 0.50 uM 0.30
20 uM oIMR0040 0.30 0.50 uM 0.30
20 ul oIMR8744 0.30 0.50 ul 0.30
20 uM oIMR8745 0.30 0.50 uM 0.30
DNA 2.00 - 2.00
Cycling 
Step # Temp °C Time Note
1 94 5 min -
2 94 30 sec -
3 50 45 sec -
4 72 30 sec repeat steps 2-4 for 35 cycles
5 72 2 min -
6 4 - hold
This genotyping protocol is used for the following strains:
 
Stock Number Strain Name
002621 B6;SJL-Tg(tetop-lacZ)2Mam/J
002856 FVB/N-Tg(TIE2-lacZ)182Sato/J
003299 B6;SWJ-Tg(TIMP3-lacZ)7Jeb/J
004141 B6;CBA-Tg(UAS-lacZ)65Rth/J
004276 B6.129-Figntm1Frk/Frk
004478 B6.129-Foxd1tm1Lai/J
004623 STOCK Tg(TCF/Lef1-lacZ)34Efu/J
005317 B6.Cg-Tg(BAT-lacZ)3Picc/J
005493 STOCK Tg(Tek-rtTA,TRE-lacZ)1425Tpr/J
005728 STOCK Tg(tetO-Ipf1,lacZ)958.1Macd/J
005801 B6.129P2-Esrratm1Dgen/J
005814 B6.129P2-Grm1tm1Dgen/J
005876 B6.CAST-(rs4222996-rs13476494)/LusJ
006229 B6.Cg-Tg(DRE-lacZ)2Gswz/J
006465 B6;CBA-Tg(CAG-lacZ-WGA)330Bbm/J
006477 B6.Cg-Tg(CAG-lacZ-WGA)330Bbm/J
006578 STOCK Myoz2tm1Eno/J
006613 STOCK Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe/J
006665 B6;129P2-Olfr151tm13(rI7)Mom/MomJ
006672 B6;CBA-Tg(Olfr16*,taulacZ)7Mom/MomJ
006686 STOCK Olfr6tm1Mom/MomJ
007972 B6;CBA-Tg(Olfr151-taulacZ)4Mom/MomJ
007973 B6;CBA-Tg(Olfr16-taulacZ)1Mom/MomJ
007974 B6;CBA-Tg(Olfr160-taulacZ)V4-7Mom/MomJ
007975 B6;CBA-Tg(OR8A1-taulacZ)1Mom/MomJ
007976 B6;CBA-Tg(Olfr713-taulacZ)4Mom/MomJ
008344 B6;DBA-Tg(Fos-tTA,Fos-EGFP*)1Mmay Tg(tetO-lacZ,tTA*)1Mmay/J
008477 STOCK Tg(RARE-Hspa1b/lacZ)12Jrt/J
008604 FVB.129(B6)-Smn1tm5(Smn1/SMN2)Mrph/J
008704 B6;129-Smn1tm6(SMN2)Mrph/J
009348 B6.129P2(Cg)-Hprttm17(Ple48-lacZ)Ems/Mmjax
012333 B6.129P2(Cg)-Hprttm45(Ple67-lacZ)Ems/Mmjax
012347 B6.129P2(Cg)-Hprttm64(Ple170-lacZ)Ems/Mmjax
012354 STOCK Hprttm66(Ple5-lacZ)Ems/Mmjax
012575 B6.129P2(Cg)-Hprttm39(Ple24-lacZ)Ems/Mmjax
012583 B6.129P2(Cg)-Hprttm68(Ple127-lacZ)Ems/Mmjax
012584 STOCK Hprttm69(Ple134-lacZ)Ems/Mmjax
012656 B6.129P2(Cg)-Hprttm70(Ple240-lacZ)Ems/Mmjax
012657 B6.129P2(Cg)-Hprttm71(Ple155-lacZ)Ems/Mmjax
012659 B6.129P2(Cg)-Hprttm73(Ple142-lacZ)Ems/Mmjax
012733 B6.129P2(Cg)-Hprttm53(CAG-lacZ)Ems/Mmjax
012734 B6.129P2(Cg)-Hprttm74(Ple232-lacZ)Ems/Mmjax
012850 B6;129P2-TardbpGt(RRB030)Byg/J
013764 STOCK Hprttm57(Ple26-lacZ)Ems/Mmjax
017524 B6;129-Tg(CMV-Bgeo,-WGA,-ALPP)1Mgmj/J
017762 B6(Cg)-Cacna1ftm1.2Sdie/J
018054 B6.129P2(Cg)-Pitx2tm1.1Dmm/J
018554 B6N(Cg)-Gpnmbtm1.1(KOMP)Vlcg/J
018561 B6N(Cg)-Ahrrtm1b(KOMP)Wtsi/J
018625 B6.FVB-Tg(Fabp4-lacZ)4Mosh/J
018627 B6;SJL-Tg(Myl1-lacZ)1Ibdml/J
019146 B6.129-Tmc1tm1.1Ajg/J
019457 STOCK Flt1tm1Jrt/FongJ
019547 B6.Cg-Cacna1ctm2Itl/J
023209 B6.129S-Sfrp1tm1Jrbn/PcmaJ
024050 B6.Cg-Tg(Hmgcr-lacZ)H253Sest/J
024896 B6N(Cg)-Akr1d1tm1.1(KOMP)Vlcg/J
304623 Tg(Fos-lacZ)34Efu/J
Genotyping resources and troubleshooting
This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.