Stock Number: 008215 Strain Name: (C57BL/6-Tg(TRAMP)8247Ng/J x FVB/NJ)F1/J

Allele: Tg(MAR) Protocol Name: Tg(MAR) Method: Fast MCA Version: 1.0 Created: 19-April -2010 Updated: 20-July -2010

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Protocol Primers

Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type 10368 - TGC ACA AGG ATG TCT GGG TA - Transgene Forward 10369 - ATA CAG GTC TCC GGC AAC AC - Transgene Reverse oIMR9020 - AAG GGA GCT GCA GTG GAG TA - Internal Positive Control Forward oIMR9021 - CCG AAA ATC TGT GGG AAG TC - Internal Positive Control Reverse

Number of Reactions

Reaction/Components  Reaction Component Volume (µl) Final Concentration Total Volume (µl) ddH2O 1.60 - 1.60 2 X KAPA SYBR Fast 6.00 1.00 X 6.00 20 uM 10368 0.60 1.00 uM 0.60 20 uM 10369 0.60 1.00 uM 0.60 20 uM oIMR9020 0.60 1.00 uM 0.60 20 uM oIMR9021 0.60 1.00 uM 0.60 DNA 2.00 - 2.00

Cycling  Step # Temp °C Time Note 1 94 5 min - 2 94 15 s - 3 61 45 s - 4 - - Go to step 2-3, 35 times 5 72 2 min - 6 10 - hold

This genotyping protocol is used for the following strains:   Stock Number Strain Name 003135 C57BL/6-Tg(TRAMP)8247Ng/J 008215 (C57BL/6-Tg(TRAMP)8247Ng/J x FVB/NJ)F1/J Genotyping resources and troubleshooting This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.