Stock Number:004178
Strain Name:B6.129(Cg)-Tg(CAG-Bgeo/GFP)21Lbe/J

Generic GFP
Fluorescent Proteins (Generic GFP)
Melt Curve Analysis
3.0
04-June -2009 05-June -2011
Notes: Melting curve analysis is done using a Roche Light Cycler 480.

This assay will NOT distinguish hemizygous from homozygous transgenic animals.
Expected Results: Transgene Tm = 89.5°C +/- 1.0°C
Internal positive control Tm = 79.4°C +/- 1.0°C
Gel Image 
Image Image file
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Protocol Primers 
Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type
oIMR0872 - AAG TTC ATC TGC ACC ACC G - Transgene
oIMR3999 - TGC TCA GGT AGT GGT TGT CG - Transgene
oIMR7338 - CTA GGC CAC AGA ATT GAA AGA TCT - Internal Positive Control Forward
oIMR7339 - GTA GGT GGA AAT TCT AGC ATC ATC C - Internal Positive Control Reverse
Reaction/Components 
Reaction Component Volume (µl) Final
Concentration
Total Volume (µl)
ddH2O 2.00 - 2.00
2 X SYBR Green Master Mix (Roche) 6.00 1.00 X 6.00
20 uM oIMR0872 0.80 1.33 uM 0.80
20 uM oIMR3999 0.80 1.33 uM 0.80
20 uM oIMR7338 0.20 0.33 uM 0.20
20 uM oIMR7339 0.20 0.33 uM 0.20
DNA 2.00 - 2.00
Cycling 
Step # Temp °C Time Note
1 94 5 -
2 94 15 sec -
3 68 1 min -
4 72 30 sec repeat steps 2-4 for 35 cycles
5 72 2 min -
6 10 - hold
This genotyping protocol is used for the following strains:
 
Stock Number Strain Name
003115 STOCK Tg(CAG-EGFP)B5Nagy/J
003116 STOCK Tg(CAG-EGFP)D4Nagy/J
003257 FVB/N-Tg(GFAPGFP)14Mes/J
003291 C57BL/6-Tg(CAG-EGFP)1Osb/J
003920 STOCK Tg(CAG-Bgeo/GFP)21Lbe/J
004178 B6.129(Cg)-Tg(CAG-Bgeo/GFP)21Lbe/J
004190 C.129-Il4tm1Lky/J
004509 B6.FVB-Tg(Itgax-DTR/EGFP)57Lan/J
004512 C.FVB-Tg(Itgax-DTR/EGFP)57Lan/J
004690 B6;FVB-Tg(Pcp2-EGFP)2Yuza/J
005029 B6.Cg-Tg(Hlxb9-GFP)1Tmj/J
005070 C57BL/6-Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck/J
005076 NOD.Cg-Tg(tetO-EGFP/FADD)1Doi/DoiJ
005104 STOCK Tg(tetO-HIST1H2BJ/GFP)47Efu/J
005282 NOD/ShiLtJ-Tg(Ins1-EGFP/GH1)14Hara/HaraJ
005418 STOCK Tg(HIST1H2BB/EGFP)1Pa/J
005630 B6.Cg-Tg(Thy1-EYFP)15Jrs/J
005706 C57BL/6-Tg(tetO-CDK5R1/GFP)337Lht/J
005738 B6.FVB-Tg(tetO-EGFP,-Tgfbr2)8Mcle/J
005854 STOCK Tg(Cp-EGFP)25Gaia/J
006043 B6;SJL-Tg(Oxt/EGFP)AI03Wsy/J
006069 B6.Cg-Tg(HIST1H2BB/EGFP)1Pa/J
006129 STOCK Tg(Zp3-EGFP)1Dean/J
006361 B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J
006567 C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ
006570 STOCK Tg(SMN2)89Ahmb Smn1tm1Msd Tg(Hlxb9-GFP)1Tmj/J
006614 B6;CBA-Tg(Thy1-CFP/COX8A)C1Lich/J
006617 B6;CBA-Tg(Thy1-CFP/COX8A)S2Lich/J
006665 B6;129P2-Olfr151tm13(rI7)Mom/MomJ
006686 STOCK Olfr6tm1Mom/MomJ
006850 STOCK Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe/J
007075 CByJ.B6-Tg(CAG-EGFP)1Osb/J
007264 C57BL/6-Tg(Sry-EGFP)92Ei Tg(Sry)4Ei Chr YPOS/EiJ
007567 C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J
007572 B6.129P2(Cg)-Rorctm2Litt/J
007615 B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J
007673 B6.Cg-Tg(Gad1-EGFP)3Gfng/J
007677 CB6-Tg(Gad1-EGFP)G42Zjh/J
007857 C57BL/6J-Tg(Eno2-YFP/Cox8a)YRwb/J
007860 C57BL/6J-Tg(Eno2-YFP/Cox8a)ZRwb/J
007894 B6.Cg-Tg(Rgs4-EGFP)4Lvt/J
007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J
007940 B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J
007967 B6.Cg-Tg(Thy1-CFP/COX8A)S2Lich/J
007977 B6;CBA-Tg(Hf/Olfr16-GFP)7Mom/MomJ
007978 B6;CBA-Tg(Hf/Olfr16-GFP)47Mom/MomJ
007979 B6;CBA-Tg(H/Olfr16-GFP)3Mom/MomJ
007981 B6;CBA-Tg(H/Olfr16-GFP)6Mom/MomJ
007982 B6;CBA-Tg(H/Olfr16-taumRFP,-tauGFP)8Mom/MomJ
007984 B6;CBA-Tg(H/Olfr16-taumCherry,-tauGFP)11Mom/MomJ
007985 B6;CBA-Tg(H/Olfr16-taumCherry,-tauGFP)13Mom/MomJ
007986 B6;CBA-Tg(H*/Olfr16-GFP)11Mom/MomJ
007987 B6;CBA-Tg(H*/Olfr16-GFP)25Mom/MomJ
008126 B6.NOD-Tg(Cd4-EGFP)1Lt/J
008173 NOD.Cg-Tg(Ins1-EGFP)1Hara/QtngJ
008344 B6;DBA-Tg(Fos-tTA,Fos-EGFP*)1Mmay Tg(tetO-lacZ,tTA*)1Mmay/J
008450 FVB-Tg(CAG-luc,-GFP)L2G85Chco/J
008549 NOD.FVB-Tg(Itgax-DTR/EGFP)57Lan/JdkJ
008605 B6;C3-Tg(CAG-DsRed,-EGFP)5Gae/J
008694 NOD/ShiLt-Tg(Foxp3-EGFP/cre)1cJbs/J
009113 B6.129P2(Cg)-Hprttm13(Ple54-EGFP)Ems/Mmjax
009159 B6;FVB-Tg(Cnp-EGFP/Rpl10a)JD368Htz/J
009353 B6.129P2(Cg)-Hprttm20(Ple53-EGFP)Ems/Mmjax
009422 NOD.Cg-Tg(Itgax-Venus)1Mnz/QtngJ
009593 C57BL/6J-Tg(Pomc-EGFP)1Low/J
009606 STOCK Tg(Six2-EGFP/cre)1Amc/J
010542 NOD.FVB-Tg(CAG-luc,-GFP)L2G85Chco/FathJ
010713 C57BL/6-Tg(tetO-GFP/tetX)5696Stl/J
010774 B6(Cg)-Calb2tm1(cre)Zjh/J
010789 B6.129P2(Cg)-Hprttm54(Ple233-EGFP)Ems/Mmjax
011106 STOCK Tg(CAG-GFP*)1Hadj/J
012350 B6;SJL-Tg(Thy1-COP4*H134R/EYFP)20Gfng/J
012643 B6.Cg-Tg(Ly6a-EGFP)G5Dzk/J
013134 B6.Cg-Tg(Col1a1*2.3-GFP)1Rowe/J
014548 B6.Cg-Tg(Slc32a1-COP4*H134R/EYFP)8Gfng/J
016251 129S.Cg-Tg(Hoxb7-EGFP)33Cos/J
016925 129;B6-Grin3b/Tmem259tm1Zhang Tg(Prnp-C19ORF6,-GFP)6Zhang/J
017466 B6.Cg-Tg(Col1a1*3.6-Topaz)2Rowe/J
017467 C57BL/6-Tg(Dmp1-Topaz)1Ikal/J
017468 STOCK Tg(Col1a1*3.6-Cyan)2Rowe/J
017469 C57BL/6-Tg(BGLAP-Topaz)1Rowe/J
017472 STOCK Tg(Acp5-CFP,Ibsp-YFP,Dmp1-RFP)1Pmay/J
017484 FVB-Tg(JPH3-GFP,-JPH3*)GXwy/J
017919 STOCK Tg(CAG-EGFP,-dsRed2/miRTDP43)6Zxu/J
017952 STOCK Tg(Isl1-EGFP*)1Slp/J
018071 FVB-Tg(Prism)1861Htz/J
018148 STOCK Tg(Slc17a8-EGFP)1Edw/SealJ
018281 STOCK Tg(Wnt7a-EGFP/cre)#Bhr/Mmjax
018306 B6.Cg-Tg(Fos-tTA,Fos-EGFP*)1Mmay/J
018549 B6N.Cg-Tg(Csf1r-EGFP)1Hume/J
018979 B6.129(Cg)-Tg(CAG-EGFP)D4Nagy/KnwJ
019494 B6.Cg-Tg(RP24-131B16/EGFP)37Ghan/J
019908 B6;129S7-Ghsrtm2Rgs/J
020942 STOCK Tg(Cp-HIST1H2BB/Venus)47Hadj/J
021187 B6;FVB-Tg(Pbsn-rtTA*M2)42Xy/J
021930 B6.129S1-Tg(CAG-EGFP)S1C2Tpo/KnwPeaJ
021937 NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(CAG-EGFP)1Osb/SzJ
022086 B6.Cg-Tg(RP24-131B16/EGFP)13Ghan/J
022486 B6.Cg-Ptprca Tg(UBC-PA-GFP)1Mnz/J
022787 B6.Cg-Tg(Chst4-EGFP)23Nrud/J
023046 B6.129S6(Cg)-Ntrk2tm2.1Ddg/J
023345 STOCK Tg(Pgk1-Ccnb1/EGFP)1Aklo/J
023426 C57BL/6-Tg(Kiss1-cre)J2-4Cfe/J
024033 B6.FVB-Tg(Shank3-EGFP)1Hzo/J
024284 STOCK Hcn4tm3.1Sev/J
350104 NOD/ShiLt-Tg(Foxp3-EGFP/cre)1Jbs/J
350105 NOD/ShiLt-Tg(Foxp3-EGFP/cre)1Jbs/J
350107 Foxp3-GFP
370368 PAX7_NLS_GFP_T2A_MER_CRE_MER BAC
671282 GcG (TG37)
671283 Drd1a (CP73)
671284 Drd2 (CP101)
671285 GcG (G6)
671286 GcG (G15)
671287 Neurod1
671288 Slc17a6
671290 GcG (TG37)
671317 Cck-391 B6
910681 ND32
911880 ST699
911885 G6
911889 ST826
911980 Glt25d2 (DU9)-F16
Genotyping resources and troubleshooting
This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.