Stock Number: 006963 Strain Name: B6(101)-Mdh1am1H/LvtJ

Allele: Mdh1am1H Protocol Name: Mdh1am1H Method: Pyrosequencing Version: 1.1 Created: 29-September-2008 Updated: 24-September-2009

Notes: This genotyping assay uses pyrosequencing technology and is run on the Biotage PSQ 96MA. The Jackson Laboratory is not posting the complete details of our pyrosequencing genotyping assays as the primers for pyrosequencing cannot be used for sequencing using more traditional methods. The wild type and mutant nucleotides and the flanking DNA sequence are provided below. Expected Results: Mutant = T/T Heterozygote = A/T Wild type = A/A

Sequence: AGTTATAAAACAAGGACAGTGTTTGGGGCTAGAGATGGCTCAGCAGTTAAGAGTGGT TGCTTTGCTTGCAGAAGGGCCAAGTTCATTCCCAGCATCCATGTCAGGTAGCTCACAC CCACCTATAACCACAGCTCCAAGAGACCTGTACATACATGTGCATGCACAGATAGACAT AGACACATAAAGAAATAAATTGTTTAATCTTTAAAACATGAAAAAAGTGAGTGTGAAG CTATGCCCTATTTTTCATGATCTGGAAGTGTAGATGAAGTGATATCTGTGACCCAGTGTG GCTGCTGAGTCCTCACAAACAGCAGTTATGCACAGTCAAGGAAGTCATGTTTTCATTG TAGTAGGTTGTTAGTTGATTGTGTAAACCAATCCCTGTAAGCTATGATAATGTAAACTT (t/a)TTCAGGGAGAGTTCGTGTCGATGGGTGTTATCTCTGATGGCAACTCCTATGGTGTC CCTGATGACCTGCTCTACTCATTCCCTGTCGTGATCAAGGTGGGGTAACATGGAAGAG GCACACATACATATACACACATATGATATTAAATATGTGTGGATATGTATAGTGTACATGT ATATAATATTTTTAGAATATATATAGATATTCCTTCCTAATGCTTTGTCTTTAATTCAATAC TGAAACTGTTCTGGAAAGCAAGATT

This genotyping protocol is used for the following strains:   Stock Number Strain Name 006963 B6(101)-Mdh1am1H/LvtJ Genotyping resources and troubleshooting This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.