Stock Number:004919
Strain Name:B6.Cg-Fcgrttm1Dcr Tg(CAG-FCGRT)276Dcr/DcrJ
Tg(FCGRT)
Tg(FCGRT)
Standard PCR
2.0
10-July -2010 09-May -2011
Notes: This assay will NOT distinguish hemizygous from homozygous transgenic animals.
Expected Results:

Transgene = 740 bp
Gel Information: Separated by gel electrophoresis on a 1.5% agarose gel.
Protocol Primers 
Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type
oIMR7718 - AGC CAA GTC CTC CGT GCT C - Transgene Forward
oIMR7719 - CTC AGA GAT GCC AGT GTT CC - Transgene Reverse
Reaction/Components 
Reaction Component Volume (µl) Final
Concentration		 Total Volume (µl)
5 M Betaine 1.66 0.69 M 1.66
ddH2O 4.92 - 4.92
10 X KAPA Rxn Buffer 1.20 1.00 X 1.20
DNA 2.00 0.00 2.00
2.5 mM dNTP 0.96 0.20 mM 0.96
20 uM oIMR7719 0.60 1.00 uM 0.60
5 U/ul KAPATaq DNA Polymerase 0.06 0.03 U/ul 0.06
20 uM oIMR7718 0.60 1.00 uM 0.60
Cycling 
Step # Temp °C Time Note
1 94 3 min -
2 94 30 sec -
3 65 30 sec -
4 72 30 sec -
5 - - Go to step 2-4, 35 times
6 72 2 min -
7 10 - hold
This genotyping protocol is used for the following strains:
 
Stock Number Strain Name
004919 B6.Cg-Fcgrttm1Dcr Tg(CAG-FCGRT)276Dcr/DcrJ
014565 B6.Cg-Fcgrttm1Dcr Tg(FCGRT)32Dcr/DcrJ
016919 B6.Cg-Rag1tm1Mom Fcgrttm1Dcr Tg(CAG-FCGRT)276Dcr/DcrJ
018441 B6.Cg-Fcgrttm1Dcr Prkdcscid Tg(FCGRT)32Dcr/DcrJ
021146 B6.Cg-Fcgrttm1Dcr Prkdcscid Tg(CAG-FCGRT)276Dcr/DcrJ
374176 B6.129X1-Fcgrttm1Dcr/DcrJ X B6.Cg-Fcgrttm1DcrTg(FCGRT)276Dcr
Genotyping resources and troubleshooting
This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.