Stock Number:005941
Strain Name:FVB/N-Tg(tetO-Aurkb,lacZ)41Kra/J

Tg(tetO-Aurkb,lacZ)41Kra
Tg(tetO-Aurkb,lacZ)41Kra
Standard PCR
1.1
03-October -2006 30-October -2009
Expected Results: Transgene = ~1100 bp
Internal positive control = 324 bp
Gel Information: Separated by gel electrophoresis on a 1.5% agarose gel.
Protocol Primers 
Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type
oIMR4189 - CTC AGA AAG AGA ACG TCT ACC - Transgene
oIMR4190 - CGA TAT GTC TCA CTG TGG CTA - Transgene
oIMR7338 - CTA GGC CAC AGA ATT GAA AGA TCT - Internal Positive Control Forward
oIMR7339 - GTA GGT GGA AAT TCT AGC ATC ATC C - Internal Positive Control Reverse
Reaction/Components 
Reaction Component Volume (µl) Final
Concentration
Total Volume (µl)
ddH2O 3.16 - 3.16
10 X AB PCR BufferII 1.20 1.00 X 1.20
25 mM MgCl2 0.96 2.00 mM 0.96
2.5 mM dNTP 0.96 0.20 mM 0.96
20 uM oIMR4189 0.60 1.00 uM 0.60
20 uM oIMR4190 0.60 1.00 uM 0.60
20 uM oIMR7338 0.40 0.67 uM 0.40
20 uM oIMR7339 0.40 0.67 uM 0.40
5 mM DNA Loading Dye 1.66 0.69 mM 1.66
5 U/ul Taq DNA Polymerase 0.06 0.03 U/ul 0.06
DNA 2.00 - 2.00
Cycling 
Step # Temp °C Time Note
1 94 3 min -
2 94 30 sec -
3 60 1 min -
4 72 1 min repeat steps 2-4 for 35 cycles
5 72 2 min -
6 10 - hold
This genotyping protocol is used for the following strains:
 
Stock Number Strain Name
005941 FVB/N-Tg(tetO-Aurkb,lacZ)41Kra/J
Genotyping resources and troubleshooting
This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.