Neuronal cell labeling
| B6.Cg-Tg(Thy1-YFP)16Jrs/J |
These mice express spectral variants of GFP (yellow-YFP) at high levels in motor neurons, sensory neurons, and in subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in nonneural cells. The transgenic insert used to make this strain is identical to that used in the construction of strain 003782 (see below). This line provides a strong and specific vital marker for axons. Fluorescence is stable to mild aldehyde fixation. Expression is strong from a mid-either for double-labeling applications or for breeding to other CFP/GFP lines. Note - see the data sheet for legal and licensing information.
Reference
Feng G, Hood R, Bernstein M, Keller-Peck C, Nguyen Q, Wallace M, Nerbonne JM, Lichtman JW, Sanes JR. 2000. Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron 28:41-51.
| B6.Cg-Tg(Thy1-YFPH)2Jrs/J |
These mice express spectral variants of GFP (yellow-YFP) at high levels in motor neurons, sensory neurons, and subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in non-neural cells. The transgenic insert used to make this strain is identical to that used in the construction of strain 003709 (see above). The primary difference between these two strains is the specific neuron subsets that express YFP. In this strain, a few motor axons are labeled in muscle tissue, allowing determination of branching pattern and definition of which muscle fibers are innervated by a single motor axon. Approximately 10-30% of sensory neurons are labeled in dorsal root ganglia. Layer 5 pyramidal cells are selectively labeled in cerebral cortex. Pyramidal neurons are selectively labeled in the hippocampus. Approximately 10-30% of retinal ganglion cells are exclusively labeled in the retina. Many (but not all) mossy fibers are strongly labeled in cerebellar cortex, but no neurons are labeled in this area. This line is a strong and specific "Golgi-like" vital marker for several widely studied neuronal subsets, with minimal labeling of nearby axons. Fluorescence appears somewhat dim at birth but brightens in adult mice. Availability of multiple spectral variants is useful for either double-labeling applications or breeding to other CFP/GFP lines. Note - see the data sheet for legal and licensing information.
Reference
Feng G, Hood R, Bernstein M, Keller-Peck C, Nguyen Q, Wallace M, Nerbonne JM, Lichtman JW, Sanes JR. 2000. Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron 28:41-51.
