Description

Strain Information

Type Congenic; Mutant Strain; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Background Strain C57BL/6J Donor Strain wild-trapped mouse Generation N45p (30-MAY-04)

Description
Mice homozygous for the pallid spontaneous mutation pa and nonagouti (a) are pink-eyed and have a light, yellow-brown coat. The coat is a little lighter than that of pink-eyed dilution homozygotes (p/p). Viability of homozygote mutant mice is slightly reduced. Some homoygotes have slightly abnormal behavior, with abnormal postural responses and head tilting due to the absence of otoliths in the sacculus and utriculus in many but not all mutant mice. The effect of pallid on behavior and otolith morphology appears to be a result of manganese deficiency. Homozygotes display defective mucopolysaccharide synthesis in the otolith matrix and a slower rate of transport of manganese, L-dopa, and L-tryptophane in the brain. Homozygotes have elevated basal and testosterone-induced levels of the kidney lysosomal enzymes b-glucuronidase, b-galactosidase, and a-mannosidase, accompanied by lowered enzyme excretion in the urine. Pallid mice have a deficiency in serum a1-antitrypsin and have been proposed as a model of genetic a1-antitrypsin deficiency. Lung lesions similar to those seen in human emphysema are found in these mice, and are attributed, like human hereditary emphysema, to a decreased capacity to inhibit serum elastase although liver a1-antitrypsin activity is normal. Pallid mice have prolonged bleeding time due to a platelet storage pool deficiency (SPD) characterized by a normal platelet number but a deficiency in the number of platelet dense granules and in the serotonin, ATP, and ADP content of the granules. Two other mouse coat color mutants, muted (mu) and mocha (Ap3d^mh), present a similar concatenation of pigment, otolith, and platelet SPD abnormalities, which also occur in human Hermansky-Pudlak syndrome.

Development
Pallid was found in a wild female mouse caught in the countryside in 1926 and brought into the laboratory of Elmer Roberts at Urbana Illinois. This female was bred with a pink-eyed, brown, nonagouti mate but no other breeding records are available from the early history of this mutation. It was maintained at the Jackson Laboratory by Dr. GD Snell in 1952. It was brother x sister bred until 1972 and then backcrossed onto C57BL/6J by ES Russell. It was cryopreserved in 1986 by mating homozygous B6.Cg-Pldn^pa males at N45 to C57BL/6J females.

Control Information

	Control
	Untyped from the colony
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying   Pldn^pa allele

000477	B10.PA-Pldnpa H3e at/SnJ
000305	B6.Cg-Fbn1Tsk +/+ Pldnpa/J
006931	B6.Cg-Pldnpa/JLlp

View Strains carrying   Pldn^pa     (3 strains)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms

Hermansky-Pudlak Syndrome; HPS - Models with phenotypic similarity to human disease where etiologies are distinct.2

2 Human genes are associated with this disease. Orthologs of those genes do not appear in the mouse genotype(s).

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Pldn^pa/Pldn^pa

        B6.Cg-Pldn^pa/J

• life span-post-weaning/aging
• premature death (MGI Ref ID J:53228)
  • survival beyond 16 months of age reduced to about 40%
• pigmentation phenotype
• abnormal melanosome morphology (MGI Ref ID J:80751)
  • immature melanosomes found in follicular melanocytes
  • increased numbers of multivesicular forms
  • striated forms more frequent as well but are usually misshapen and with irregular pigment
  • do not increase in size with maturation
• diluted coat color (MGI Ref ID J:99881)
  • display a generalized pigmented dilution
• ocular albinism (MGI Ref ID J:141035)
  • near total iris albinism
• respiratory system phenotype
• abnormal lung morphology (MGI Ref ID J:53228)
  • "honeycomb" effect on lung architecture
• abnormal respiratory alveoli morphology (MGI Ref ID J:88021)
  • by 16 months of age, enlarged airspaces and distruction of alveolar tissue
• abnormal respiratory mechanics (MGI Ref ID J:88021)
  • lung reactance values decrease with age relative to controls
• homeostasis/metabolism phenotype
• abnormal platelet physiology (MGI Ref ID J:7327)
  • platelet ATP and ADP levels are much lower than in C57BL/6J controls
• decreased platelet serotonin level (MGI Ref ID J:7327)
  • platetelet serotonin levels less than 1% of normal
• abnormal urine enzyme level (MGI Ref ID J:6219)
  • reduced secretion of lysosomal enzymes into the urine of testosterone treated mice
• increased bleeding time (MGI Ref ID J:7327)
  • greater than 15 minutes
• platelet storage pool deficiency (MGI Ref ID J:7327)
  • platelet ATP about half normal
  • platelet ADP a third to a half normal
  • reduced serotonin release after thrombin activation
  • lysosomal enzyme secretion after thrombin activation about half normal
• renal/urinary system phenotype
• abnormal kidney physiology (MGI Ref ID J:6219)
  • kidney beta-glucuronidase levels increased 2.5X
  • kidney levels of beta-galactosidase and alpha-mannosidase increased
  • increased rate of lysosomal enzyme synthesis in kidneys
• abnormal urine enzyme level (MGI Ref ID J:6219)
  • reduced secretion of lysosomal enzymes into the urine of testosterone treated mice
• behavior/neurological phenotype
• ataxia (MGI Ref ID J:5215)
  • exaggerated ataxia in some mice after a swim test
• impaired balance (MGI Ref ID J:5215)
  • sometimes seen to roll over at the end of a swim test
• head tilt (MGI Ref ID J:5215)
  • seen in about 25% of homozygotes
• impaired swimming (MGI Ref ID J:89392)
  • erratic swimming pattern in some mice with head tilt
  • 55/99 mice unable to swim - related to absence of normal otoliths
• tonic seizures (MGI Ref ID J:5215)
  • momentary, in mice displaying head tilt when first placed in water for a swim test
• hearing/vestibular/ear phenotype
• abnormal linear vestibular evoked potential (MGI Ref ID J:89392)
  • variable defects in latencies, amplitudes and thresholds of VESPs, dependent on amounts of otoconia present
  • mice with absent otoconia show absent vestibular compound action potentials
• abnormal otolith morphology (MGI Ref ID J:89392)
  • variable presence of otoliths - from normal to complete absence
  • otolith development improves by maternal feeding with increased levels of manganese during early gestation
• head tilt (MGI Ref ID J:5215)
  • seen in about 25% of homozygotes
• hematopoietic system phenotype
• abnormal platelet dense granule number (MGI Ref ID J:7327)
  • fewer dense granules per platelet
• abnormal platelet physiology (MGI Ref ID J:7327)
  • platelet ATP and ADP levels are much lower than in C57BL/6J controls
• decreased platelet serotonin level (MGI Ref ID J:7327)
  • platetelet serotonin levels less than 1% of normal
• platelet storage pool deficiency (MGI Ref ID J:7327)
  • platelet ATP about half normal
  • platelet ADP a third to a half normal
  • reduced serotonin release after thrombin activation
  • lysosomal enzyme secretion after thrombin activation about half normal
• nervous system phenotype
• tonic seizures (MGI Ref ID J:5215)
  • momentary, in mice displaying head tilt when first placed in water for a swim test
• cellular phenotype
• abnormal lysosome physiology (MGI Ref ID J:6801)
  • significant increase in lysosomal enzyme activity of beta-galactosidase and beta-glucuronidase, and to a lesser extent N-acetyl-beta-hexoseaminidase, in kidney extracts
• immune system phenotype
• abnormal NK cell physiology (MGI Ref ID J:6801)
  • lower natural killer cell activity
• skin/coat/nails phenotype
• *normal* skin/coat/nails phenotype (MGI Ref ID J:68448)
  • electron microscopy of the skin shows regular collagen bundles and no excess collagen deposition
• diluted coat color (MGI Ref ID J:99881)
  • display a generalized pigmented dilution
• vision/eye phenotype
• ocular albinism (MGI Ref ID J:141035)
  • near total iris albinism

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Pldn^pa/Pldn^pa

        involves: wild-derived

• skin/coat/nails phenotype
• diluted coat color (MGI Ref ID J:13138)
  • pale agouti
• vision/eye phenotype
• reduced eye pigmentation (MGI Ref ID J:13138)
  • pink eyes
• pigmentation phenotype
• diluted coat color (MGI Ref ID J:13138)
  • pale agouti
• reduced eye pigmentation (MGI Ref ID J:13138)
  • pink eyes

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Pldn^pa related

Dermatology Research
Color and White Spotting Defects

Hematological Research
Platelet Defects (platelet storage pool deficiency)

Internal/Organ Research
Kidney Defects (lysosomal enzyme abnormalities)
Lung Defects (emphysema)

Neurobiology Research
Vestibular and Hearing Defects

Sensorineural Research
Vestibular and Hearing Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Pldnpa
Allele Name		pallid
Allele Type		Spontaneous
Common Name(s)		pa;
Strain of Origin		wild
Gene Symbol and Name		Pldn, pallidin
Chromosome		2
Gene Common Name(s)		BLOC-1; BLOC-1 subunit; MGC114534; PA; PALLID; pa; pallid;
Molecular Note		The C to T substitution at nucleotide 787 introduces a premature stop codon in pallid mice. Through Northern analysis, a single mRNA of about 2.5kb was found in reduced levels compared to wild-type. [MGI Ref ID J:2185] [MGI Ref ID J:64907]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Additional References

Gwynn B; Korsgren C; Cohen CM; Ciciotte SL; Peters LL. 1997. The gene encoding protein 4.2 is distinct from the mouse platelet storage pool deficiency mutation pallid. Genomics 42(3):532-5. [PubMed: 9205130]  [MGI Ref ID J:38773]

Jones SM; Erway LC; Johnson KR; Yu H; Jones TA. 2004. Gravity receptor function in mice with graded otoconial deficiencies. Hear Res 191(1-2):34-40. [PubMed: 15109702]  [MGI Ref ID J:89392]

White RA; Peters LL; Adkison LR; Korsgren C; Cohen CM; Lux SE. 1992. The murine pallid mutation is a platelet storage pool disease associated with the protein 4.2 (pallidin) gene. Nat Genet 2(1):80-3. [PubMed: 1284644]  [MGI Ref ID J:2185]

Pldn^pa related

Alexander JJ; Jacob A; Cunningham P; Hensley L; Quigg RJ. 2008. TNF is a key mediator of septic encephalopathy acting through its receptor, TNF receptor-1. Neurochem Int 52(3):447-56. [PubMed: 17884256]  [MGI Ref ID J:140283]

Anderson MG; Hawes NL; Trantow CM; Chang B; John SW. 2008. Iris phenotypes and pigment dispersion caused by genes influencing pigmentation. Pigment Cell Melanoma Res 21(5):565-78. [PubMed: 18715234]  [MGI Ref ID J:141035]

Bayle J; Fitch J; Jacobsen K; Kumar R; Lafyatis R; Lemaire R. 2008. Increased expression of Wnt2 and SFRP4 in Tsk mouse skin: role of Wnt signaling in altered dermal fibrillin deposition and systemic sclerosis. J Invest Dermatol 128(4):871-81. [PubMed: 17943183]  [MGI Ref ID J:135507]

Bodmer WF. 1961. Viability effects and recombination differences in a linkage test with pallid and fidget in the house mouse Heredity 16:485-95.  [MGI Ref ID J:2]

Bossi G; Booth S; Clark R; Davis EG; Liesner R; Richards K; Starcevic M; Stinchcombe J; Trambas C; Dell'Angelica EC; Griffiths GM. 2005. Normal lytic granule secretion by cytotoxic T lymphocytes deficient in BLOC-1, -2 and -3 and myosins Va, VIIa and XV. Traffic 6(3):243-51. [PubMed: 15702992]  [MGI Ref ID J:105404]

Chan WT; Sherer NM; Uchil PD; Novak EK; Swank RT; Mothes W. 2008. Murine leukemia virus spreading in mice impaired in the biogenesis of secretory lysosomes and Ca2+-regulated exocytosis. PLoS ONE 3(7):e2713. [PubMed: 18629000]  [MGI Ref ID J:139279]

Deol MS. 1976. Deficiencies of the inner ear in the mouse and their origin Colloq Int (Collques Internationaux) C.N.R.S. 266:163-171.  [MGI Ref ID J:136112]

Dimitriu-Bona A; Fillit H. 1993. Studies of the cellular immune response to heparan sulfate proteoglycan in the tight skin mouse. Cell Immunol 150(2):321-32. [PubMed: 8370075]  [MGI Ref ID J:14526]

Erway LC; Fraser AS; Hurley LS. 1971. Prevention of congenital otolith defect in pallid mutant mice by manganese supplementation. Genetics 67(1):97-108. [PubMed: 5559839]  [MGI Ref ID J:5215]

Falcon-Perez JM; Dell'Angelica EC. 2002. The pallidin (Pldn) gene and the role of SNARE proteins in melanosome biogenesis. Pigment Cell Res 15(2):82-6. [PubMed: 11936273]  [MGI Ref ID J:76190]

Falcon-Perez JM; Starcevic M; Gautam R; Dell'Angelica EC. 2002. BLOC-1, a novel complex containing the pallidin and muted proteins involved in the biogenesis of melanosomes and platelet-dense granules. J Biol Chem 277(31):28191-9. [PubMed: 12019270]  [MGI Ref ID J:88019]

Gardi C; Cavarra E; Calzoni P; Marcolongo P; de Santi M; Martorana PA; Lungarella G. 1994. Neutrophil lysosomal dysfunctions in mutant C57 Bl/6J mice: interstrain variations in content of lysosomal elastase, cathepsin G and their inhibitors. Biochem J 299(Pt 1):237-45. [PubMed: 8166647]  [MGI Ref ID J:17583]

Huang L; Kuo YM; Gitschier J. 1999. The pallid gene encodes a novel, syntaxin 13-interacting protein involved in platelet storage pool deficiency. Nat Genet 23(3):329-32. [PubMed: 10610180]  [MGI Ref ID J:64907]

Hurley LS. 1976. Interaction of genes and metals in development. Fed Proc 35(11):2271-5. [PubMed: 133823]  [MGI Ref ID J:5680]

Ito S; Bartolak-Suki E; Shipley JM; Parameswaran H; Majumdar A; Suki B. 2006. Early emphysema in the tight skin and pallid mice: roles of microfibril-associated glycoproteins, collagen, and mechanical forces. Am J Respir Cell Mol Biol 34(6):688-94. [PubMed: 16439805]  [MGI Ref ID J:122617]

Jones SM; Erway LC; Johnson KR; Yu H; Jones TA. 2004. Gravity receptor function in mice with graded otoconial deficiencies. Hear Res 191(1-2):34-40. [PubMed: 15109702]  [MGI Ref ID J:89392]

Kakizoe E; Shiota N; Tanabe Y; Shimoura K; Kobayashi Y; Okunishi H. 2001. Isoform-selective upregulation of mast cell chymase in the development of skin fibrosis in scleroderma model mice. J Invest Dermatol 116(1):118-23. [PubMed: 11168806]  [MGI Ref ID J:68449]

Keil M; Lungarella G; Cavarra E; van Even P; Martorana PA. 1996. A scanning electron microscopic investigation of genetic emphysema in tight-skin, pallid, and beige mice, three different C57 BL/6J mutants. Lab Invest 74(2):353-62. [PubMed: 8780155]  [MGI Ref ID J:31735]

Lemaire R; Korn JH; Schiemann WP; Lafyatis R. 2004. Fibulin-2 and fibulin-5 alterations in tsk mice associated with disorganized hypodermal elastic fibers and skin tethering. J Invest Dermatol 123(6):1063-9. [PubMed: 15610515]  [MGI Ref ID J:94185]

Lyon MF. 1953. Absence of otoliths in the mouse: An effect of the pallid mutant J Genet 51:638-50.  [MGI Ref ID J:19343]

Martorana PA; Brand T; Gardi C; van Even P; de Santi MM; Calzoni P; Marcolongo P; Lungarella G. 1993. The pallid mouse. A model of genetic alpha 1-antitrypsin deficiency. Lab Invest 68(2):233-41. [PubMed: 8441253]  [MGI Ref ID J:4098]

Matsushita T; Fujimoto M; Hasegawa M; Matsushita Y; Komura K; Ogawa F; Watanabe R; Takehara K; Sato S. 2007. BAFF antagonist attenuates the development of skin fibrosis in tight-skin mice. J Invest Dermatol 127(12):2772-80. [PubMed: 17581616]  [MGI Ref ID J:127329]

McGaha T; Saito S; Phelps RG; Gordon R; Noben-Trauth N; Paul WE; Bona C. 2001. Lack of skin fibrosis in tight skin (TSK) mice with targeted mutation in the interleukin-4R alpha and transforming growth factor-beta genes. J Invest Dermatol 116(1):136-43. [PubMed: 11168809]  [MGI Ref ID J:68448]

McGarry MP; Borchers M; Novak EK; Lee NA; Ohtake PJ; Lee JJ; Swank RT. 2002. Pulmonary pathologies in pallid mice result from nonhematopoietic defects. Exp Mol Pathol 72(3):213-20. [PubMed: 12009785]  [MGI Ref ID J:88021]

McGarry MP; Reddington M; Novak EK; Swank RT. 1999. Survival and lung pathology of mouse models of Hermansky-Pudlak syndrome and Chediak-Higashi syndrome. Proc Soc Exp Biol Med 220(3):162-8. [PubMed: 10193444]  [MGI Ref ID J:53228]

Moore KJ; Swing DA; Copeland NG; Jenkins NA. 1990. Interaction of the murine dilute suppressor gene (dsu) with fourteen coat color mutations [published erratum appears in Genetics 1990 Sep;126(1):285] Genetics 125(2):421-30. [PubMed: 2379821]  [MGI Ref ID J:29467]

Nazarian R; Falcon-Perez JM; Dell'Angelica EC. 2003. Biogenesis of lysosome-related organelles complex 3 (BLOC-3): a complex containing the Hermansky-Pudlak syndrome (HPS) proteins HPS1 and HPS4. Proc Natl Acad Sci U S A 100(15):8770-5. [PubMed: 12847290]  [MGI Ref ID J:99881]

Nguyen T; Novak EK; Kermani M; Fluhr J; Peters LL; Swank RT; Wei ML. 2002. Melanosome morphologies in murine models of hermansky-pudlak syndrome reflect blocks in organelle development. J Invest Dermatol 119(5):1156-64. [PubMed: 12445206]  [MGI Ref ID J:80751]

Novak EK; Hui SW; Swank RT. 1984. Platelet storage pool deficiency in mouse pigment mutations associated with seven distinct genetic loci. Blood 63(3):536-44. [PubMed: 6696991]  [MGI Ref ID J:7327]

Novak EK; McGarry MP; Swank RT. 1985. Correction of symptoms of platelet storage pool deficiency in animal models for Chediak-Higashi syndrome and Hermansky-Pudlak syndrome. Blood 66(5):1196-201. [PubMed: 3902123]  [MGI Ref ID J:8056]

Novak EK; Swank RT. 1979. Lysosomal dysfunctions associated with mutations at mouse pigment genes. Genetics 92(1):189-204. [PubMed: 115747]  [MGI Ref ID J:6219]

Orn A; Hakansson EM; Gidlund M; Ramstedt U; Axberg I; Wigzell H; Lundin LG. 1982. Pigment mutations in the mouse which also affect lysosomal functions lead to suppressed natural killer cell activity. Scand J Immunol 15(3):305-10. [PubMed: 7089489]  [MGI Ref ID J:6801]

Phelps RG; Daian C; Shibata S; Fleischmajer R; Bona CA. 1993. Induction of skin fibrosis and autoantibodies by infusion of immunocompetent cells from tight skin mice into C57BL/6 Pa/Pa mice. J Autoimmun 6(6):701-18. [PubMed: 8155252]  [MGI Ref ID J:16435]

Roberts E. 1931. A new mutation in the house mouse (Mus musculus). Science 74:569.  [MGI Ref ID J:13138]

Salazar G; Craige B; Styers ML; Newell-Litwa KA; Doucette MM; Wainer BH; Falcon-Perez JM; Dell'Angelica EC; Peden AA; Werner E; Faundez V. 2006. BLOC-1 complex deficiency alters the targeting of adaptor protein complex-3 cargoes. Mol Biol Cell 17(9):4014-26. [PubMed: 16760431]  [MGI Ref ID J:114481]

Sgonc R; Dietrich H; Sieberer C; Wick G; Christner PJ; Jimenez SA. 1999. Lack of endothelial cell apoptosis in the dermis of tight skin 1 and tight skin 2 mice. Arthritis Rheum 42(3):581-4. [PubMed: 10088787]  [MGI Ref ID J:55591]

Shrader RE; Erway LC; Hurley LS. 1973. Mucopolysaccharide synthesis in the developing inner ear of manganese-deficient and pallid mutant mice. Teratology 8(3):257-66. [PubMed: 4272079]  [MGI Ref ID J:5402]

Silvers WK. 1979. The Coat Colors of Mice; A Model for Mammalian Gene Action and Interaction. In: The Coat Colors of Mice. Springer-Verlag, New York.  [MGI Ref ID J:78801]

Swank RT; Novak EK; McGarry MP; Rusiniak ME; Feng L. 1998. Mouse models of Hermansky Pudlak syndrome: a review. Pigment Cell Res 11(2):60-80. [PubMed: 9585243]  [MGI Ref ID J:88018]

Swank RT; Novak EK; McGarry MP; Zhang Y; Li W; Zhang Q; Feng L. 2000. Abnormal vesicular trafficking in mouse models of Hermansky-Pudlak syndrome. Pigment Cell Res 13 Suppl 8:59-67. [PubMed: 11041359]  [MGI Ref ID J:103794]

Swank RT; Reddington M; Howlett O; Novak EK. 1991. Platelet storage pool deficiency associated with inherited abnormalities of the inner ear in the mouse pigment mutants muted and mocha. Blood 78(8):2036-44. [PubMed: 1912584]  [MGI Ref ID J:29151]

Trune DR; Lim DJ. 1983. The behavior and vestibular nuclear morphology of otoconia-deficient pallid mutant mice. J Neurogenet 1(1):53-69. [PubMed: 6681441]  [MGI Ref ID J:7946]

Weihrauch D; Xu H; Shi Y; Wang J; Brien J; Jones DW; Kaul S; Komorowski RA; Csuka ME; Oldham KT; Pritchard KA. 2007. Effects of D-4F on vasodilation, oxidative stress, angiostatin, myocardial inflammation, and angiogenic potential in tight-skin mice. Am J Physiol Heart Circ Physiol 293(3):H1432-41. [PubMed: 17496220]  [MGI Ref ID J:126106]

White RA; Peters LL; Adkison LR; Korsgren C; Cohen CM; Lux SE. 1992. The murine pallid mutation is a platelet storage pool disease associated with the protein 4.2 (pallidin) gene. Nat Genet 2(1):80-3. [PubMed: 1284644]  [MGI Ref ID J:2185]

de Santi MM; Martorana PA; Cavarra E; Lungarella G. 1995. Pallid mice with genetic emphysema. Neutrophil elastase burden and elastin loss occur without alteration in the bronchoalveolar lavage cell population. Lab Invest 73(1):40-7. [PubMed: 7603039]  [MGI Ref ID J:27224]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Untyped from the colony
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.