Strain Name:

B6.Cg-Atp7aMo-to/J

Stock Number:

000053

Availability:

Repository-Cryopreserved

Description

Strain Information

Type Congenic; Mutant Strain;
Specieslaboratory mouse
Background Strain C57BL/6J
Donor Strain Mixed stock
GenerationN87

Development
The tortoise shell mutation (Atp7aMo-to) arose spontaneously in a non-inbred obese stock in 1952 at the Jackson Laboratory. A pair of obese heterozygotes on a miscellaneous background produced a single tortoise shell appearing female which was mated to a normal appearing male sibling. A tortoise shell female offspring was mated to a C57BL/6J male and the stock was then sibling mated using tortoise shell females bred to wildtype males since tortoise shell males die before birth. The stock was at F17 in 1960. It was taken by Dr. Elizabeth Russell and then backcrossed onto the C57BL/6J background. It was cryopreserved in 1979 by mating tortoise shell females at N79-88 to C57BL/6J males. The stock was refrozen in 1984 by mating tortoise shell females at N86 from the frozen embryo repository to C57BL/6J males.

Related Strains

Strains carrying   Atp7aMo-to allele
000623   TR/DiEiJ
View Strains carrying   Atp7aMo-to     (1 strain)

Strains carrying other alleles of Atp7a
000535   B6.Cg-Atp7aMo-blo/J
001381   B6.Cg-Atp7aMo-pew2J/J
002062   B6C3Fe a/a-Atp7aMo-8J/J
002044   B6Ei.Cg-Atp7aMo-blo/J
002566   C57BL/6-Atp7aMo-br/J
000813   CBA/J-Atp7aMo-pew/J
View Strains carrying other alleles of Atp7a     (6 strains)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

Mammalian Phenotype Terms assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Atp7aMo-to/Atp7a+

        Background Not Specified
  • cardiovascular system phenotype
  • aortic aneurysm (MGI Ref ID J:5397)
    • 25% of females display aortic aneurysms and 17% show S-shaped lesions (lesions/aneurysms involve the thoracic and abdominal aorta and its branches)

Atp7aMo-to/Y

        Background Not Specified
  • cardiovascular system phenotype
  • aortic aneurysm (MGI Ref ID J:5397)
    • 81% of males develop aortic aneurysms

Research Applications

This mouse can be used to support research in many areas including:

Atp7aMo-to related

Dermatology Research
Color and White Spotting Defects

Developmental Biology Research
Defects in Extracellular Matrix Molecules

Metabolism Research

Mouse/Human Gene Homologs
Menkes syndrome

Neurobiology Research
Metabolic Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol Atp7aMo-to
Allele Name tortoiseshell
Strain of Originobese stock
Gene Symbol and Name Atp7a, ATPase, Cu++ transporting, alpha polypeptide
Chromosome X
Gene Common Name(s) Blo; FLJ17790; MK; MNK; Menkes protein; Mo; blotchy; br; mottled;
General Note Atp7aMo-to, tortoiseshell, semidominant. Arising spontaneously in an obese stock, the tortoiseshell mutation was considered to be a mottled locus allele because of its interaction with other alleles at the locus and because it shows about the same linkage relation with Zic3 (bent tail) as Atp7aMo (J:13382). Heterozygous females resemble Atp7a/+ females in color. The vibrissae are slightly wavy and the coat has a slightly silky texture. The distribution of patches of wild-type and white color is different from the distribution of patches of wild-type and wavy hair, suggesting that Atp7aMo-to probably acts independently in melanoblasts and hair follicles (J:15324). Most hemizygous males die before birth (J:24988), but a few are stillborn (J:12963). Using as parent a male presumed to be chimeric for Atp7aMo-to/Y and +/Y, Grahn et al. (J:12963) showed that homozygous Atp7aMo-to/Atp7aMo-to and heterozygous Atp7aMo-to/Atp7aMo-dp females die in utero, while heterozygous Atp7aMo-to/Atp7aMo-blo and Atp7aMo-to/Atp7aMo-br females reach term, but die by 15 days of age. The elastic lamina of the aorta is defective in stillbornhemizygous Atp7aMo-to males and in 40 per cent of Atp7aMo-to/+ female heterozygotes.
Molecular Note A spontaneous mutation that arose at The Jackson Laboratory in 1952.

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Additional References

Atp7aMo-to related

Dickie MM. 1954. The tortoise shell house mouse J Hered 45:158 & 190.  [MGI Ref ID J:24988]

Erdman MD; Verley FA; Bondari K. 1987. Effects of the sex-linked prenatal lethal gene tortoise (Moto) on reproduction and growth in the mouse. Growth 51(2):189-97. [PubMed: 3678930]  [MGI Ref ID J:79327]

Gacheru S; McGee C; Uriu-Hare JY; Kosonen T; Packman S; Tinker D; Krawetz SA; Reiser K; Keen CL; Rucker RB. 1993. Expression and accumulation of lysyl oxidase, elastin, and type I procollagen in human Menkes and mottled mouse fibroblasts. Arch Biochem Biophys 301(2):325-9. [PubMed: 8096378]  [MGI Ref ID J:36272]

Grahn D; Fry RJM; Hamilton KF. 1969. Genetic and pathological analysis of the sex-linked allelic series, mottled, in the mouse. Genetics 61:s22-s23.  [MGI Ref ID J:12963]

Lane PW. 1960. Tortoiseshell (To) and bent tail (Bn) Mouse News Lett 23:36.  [MGI Ref ID J:13382]

Mintz B. 1970. Gene expression in allophenic mice. In: Control Mechanisms in the Expression of Cellular Phenotypes. Symposia of the International Society for Cell Biology. Academic Press, New York, NY.  [MGI Ref ID J:15324]

Rowe DW; McGoodwin EB; Martin GR; Sussman MD; Grahn D; Faris B; Franzblau C. 1974. A sex-linked defect in the cross-linking of collagen and elastin associated with the mottled locus in mice. J Exp Med 139(1):180-92. [PubMed: 4808708]  [MGI Ref ID J:5397]

Sheedlo HJ; Beck ML. 1981. Copper localization in the duodenal mucosa of heterozygous tortoiseshell (Moto/+) female mice (Mus musculus). Genet Res 38(3):333-6. [PubMed: 7333461]  [MGI Ref ID J:79328]

Sheedlo HJ; Beck ML. 1982. SEM analysis of body hairs and whiskers of heterozygous tortoiseshell (Moto/+) female mice (Mus musculus). J Anat 135(Pt 1):211-6. [PubMed: 7130053]  [MGI Ref ID J:79326]

Sundberg JP (ed.). 1994. . In: Handbook of Mouse Mutations with Skin and Hair Abnormalities: Animal Models and Biomedical Tools. CRC Press, Boca Raton.  [MGI Ref ID J:30359]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations             View   International   Pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $1900.00
*Price(s) in US dollars ($)

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • Genomic DNA is available for this strain from the Mouse DNA Resource.

Pricing for International shipping destinations             View   USA, Canada and Mexico   Pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $2470.00
*Price(s) in US dollars ($)

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • Genomic DNA is available for this strain from the Mouse DNA Resource.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering and Purchasing Information

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Contact Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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