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Type Mutant Stock; Spontaneous Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation N4F1p Appearance
Pax1un and we: black and tan, wavy tail, wavy fur
Related Genotype: Pax1un we at/Pax1un we ?
Pax1un and we: black and tan, normal tail and fur
Related Genotype: + + a/we Pax1un at or + + ?/? ? at
Pax1un and we: black, normal tail and fur
Related Genotype: + + a/? ? a
at: black and tan, wavy tail, wavy fur
Related Genotype: at/a Pax1un/a Pax1un we/we
at: black and tan
Related Genotype: at/aDescription
The affected mutant (we Pax1un at/we Pax1un a?) has a wavy coat, wavy tail, and is black and tan.Development
A mixed stock containing at, Pax1un, we, and other mutations was imported into The Jackson Laboratory from Edinburgh in 1952. The at Pax1un we line was maintained by sibling matings until F7 then outcrossed to C57BL/6J and again sibling mated to F19 before being outcrossed again to C57BL/6J and then sibling mated to F44. It was lastly outcrossed to the hybrid B6C3Fe-a/a and then maintained by cross-intercross to this F1 until NE15. In 1979 embryos were generated for cryopreservation by breeding heterozygous females (at Pax1un we/a + +) with homozygous males (at Pax1un we/at Pax1un we).
| Control | ||
|---|---|---|
| Untyped from the colony | ||
| Considerations for Choosing Controls | ||
Strains carrying Pax1un allele
000419 B10.UW-H3b we Pax1un at/SnJ View Strains carrying Pax1un (1 strain)
Strains carrying at allele
000477 B10.PA-Pldnpa H3e at/SnJ 000419 B10.UW-H3b we Pax1un at/SnJ 000262 LS/LeJ View Strains carrying at (3 strains)
Strains carrying we allele
000419 B10.UW-H3b we Pax1un at/SnJ 000288 B6CBACa Aw-J/A-we a Mafbkr/J View Strains carrying we (2 strains)
Strains carrying other alleles of a
View Strains carrying other alleles of a (161 strains)
Strains carrying other alleles of we
003656 AKR/J-we4J/J 000475 B10.129-weBkr/CyJ View Strains carrying other alleles of we (2 strains)
View Research Applications
Research Applications
This mouse can be used to support research in many areas including:Pax1un related
at relatedDevelopmental Biology Research
Skeletal Defects
we relatedDermatology Research
Color and White Spotting Defects
Skin and Hair Texture Defects
| Allele Symbol | Pax1un | ||
|---|---|---|---|
| Allele Name | undulated | ||
| Allele Type | Spontaneous | ||
| Common Name(s) | un; | ||
| Strain of Origin | fancier's mice | ||
| Gene Symbol and Name | Pax1, paired box gene 1 | ||
| Chromosome | 2 | ||
| Gene Common Name(s) | HUP48; Pax-1; hbs; hunchback; un; undulated; wavy tail; wt; | ||
| General Note | This mutation was found in mice obtained from a Cambridge, England, fancier (J:314). DNA analysis determined that the undulated mutation represented a substitution of glycine for serine at position 15 of the paired box motif in Pax1 (J:9424). This substitution markedly decreases the DNA-binding ability of the Pax1 protein and changes its DNA specificity (J:31264).Homozygotes have a shortened and usually kinked tail. The caudal vertebrae are reduced in size but not in number. The tail kinks can easily be flattened out with the fingers but immediately return when the tail is released. Some homozygotes have marked kyphosis of the lower thoracic and upper lumbar region. The vertebrae are abnormally formed over the whole spine. The acromion process of the scapula is reduced or absent (J:13037). The anomalies can be traced to the 11th day of gestation. The condensations of mesenchyme cranial to the sclerotomic fissure are smaller than normal. Instead of joining with the primitive centra in front of them to form the body of the vertebra, they remain with the material posterior to the sclerotomic fissure and enter into the intervertebral disk. The vertebrae are thus smaller and the disks larger than normal (J:13043).The double-mutant mouse homozygous for both Pax1un and patch (PdgfraPh) exhibits an extreme form of spina bifida. Neither the undulated nor the patch phenotypes are more extreme in the double mutant, and neither mutation alone has spina bifida effects (J:28396). | ||
| Molecular Note | A point mutation results in a substitution of glycine for serine at position 15 of the paired box motif. [MGI Ref ID J:9424] | ||
| Allele Symbol | at | ||
| Allele Name | black and tan | ||
| Allele Type | Spontaneous | ||
| Strain of Origin | English fancy stock | ||
| Gene Symbol and Name | a, nonagouti | ||
| Chromosome | 2 | ||
| Gene Common Name(s) | AGSW; AGTI; AGTIL; ASP; As; MGC126092; MGC126093; SHEP9; agouti; agouti signal protein; agouti suppressor; | ||
| General Note | The black and tan mutation was found by Dunn (J:15011) in a strain obtained from an English fancier. The back is black and the belly cream or yellow. Effects are recessive to A on back but dominant on belly. A/at mice resemble Aw/-.Dermal--epidermal recombination experiments have shown that the regional differences in color are determined by gene action in the dermis (J:5672).This allele is recessive to A and Aw on the dorsum and dominant to all agouti alleles on the ventrum except for Aw from which it is indistinguishable (J:78801) . | ||
| Molecular Note | This allele comprises a 6 kb insertion containing a retrovirus-like transposable element VL30 and a single 526 bp repeat into the first intron of the agouti gene at the same position as for alleles a and Aw. [MGI Ref ID J:16984] | ||
| Allele Symbol | we | ||
| Allele Name | wellhaarig | ||
| Allele Type | Spontaneous | ||
| Strain of Origin | Agnes Bluhm's stocks | ||
| General Note | Arose as a spontaneous mutation in the stocks of Agnes Bluhm. Homozygotes are fully fertile. They have curly whiskers at 2 to 3 days of age and a wavy first coat, most strongly evident between 10 and 21 days. In later coats the waviness is lost. The hairs have a lower average diameter then those of normal mice (J:208). we has been shown to be on Chr 2 closely linked to pallid (Pldn) and has been bred in coupling with Pldn (J:247) with no interaction between the two genes. However, Graff et al. (J:8296) reported a cross between we/we and pa/pa mice that produced F2 pallid wellhaarig offspring in much higher than expected frequency, all showing a severe skeletal abnormality and early death. The authors hypothesized that the effect was due to complementary action of genes in the pa-we segment and that the F2 pallid wellhaarig mice were pa we/pa + in genotype, accounting for the excess of this class in the F2. Why the results of this cross differed from those of similar previous crosses is not clear. | ||
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Optimizing PCR Protocols
Pax1un relatedat relatedAubin J; Lemieux M; Moreau J; Lapointe J; Jeannotte L. 2002. Cooperation of Hoxa5 and Pax1 Genes during Formation of the Pectoral Girdle. Dev Biol 244(1):96-113. [PubMed: 11900462] [MGI Ref ID J:75823]
Balling R; Deutsch U; Gruss P. 1988. undulated, a mutation affecting the development of the mouse skeleton, has a point mutation in the paired box of Pax 1. Cell 55(3):531-5. [PubMed: 3180219] [MGI Ref ID J:9424]
Chalepakis G; Fritsch R; Fickenscher H; Deutsch U; Goulding M; Gruss P. 1991. The molecular basis of the undulated/Pax-1 mutation. Cell 66(5):873-84. [PubMed: 1889089] [MGI Ref ID J:31264]
Dietrich S; Gruss P. 1995. undulated phenotypes suggest a role of Pax-1 for the development of vertebral and extravertebral structures. Dev Biol 167(2):529-48. [PubMed: 7875377] [MGI Ref ID J:23331]
Gruneberg H. 1950. Genetical studies on the skeleton of the mouse. II. Undulated and its "modifiers". J Genet 50:142-173. [MGI Ref ID J:13037]
Gruneberg H. 1954. Genetical studies on the skeleton of the mouse. XII. The development of undulated. J Genet 52(2):441-455. [MGI Ref ID J:13043]
Helwig U; Imai K; Schmahl W; Thomas BE; Varnum DS; Nadeau JH; Balling R. 1995. Interaction between undulated and Patch leads to an extreme form of spina bifida in double-mutant mice. Nat Genet 11(1):60-3. [PubMed: 7550316] [MGI Ref ID J:28396]
Johnson DR. 1976. The interfrontal bone and mutant genes in the mouse. J Anat 121(3):507-13. [PubMed: 1018005] [MGI Ref ID J:5776]
Joosten PH; van Zoelen EJ; Murre C. 2005. Pax1/E2a Double-Mutant Mice Develop Non-Lethal Neural Tube Defects that Resemble Human Malformations. Transgenic Res 14(6):983-7. [PubMed: 16315099] [MGI Ref ID J:103567]
Quondamatteo F; Zieger J; Gotz W; Miosge N; Herken R. 2000. Extensive glycosylation changes revealed by lectin histochemistry in morphologically normal prenatal tissues of the mouse mutant undulated (un/un). Anat Rec 258(3):243-51. [PubMed: 10705344] [MGI Ref ID J:61387]
Timmons PM; Wallin J; Rigby PW; Balling R. 1994. Expression and function of Pax 1 during development of the pectoral girdle. Development 120(10):2773-85. [PubMed: 7607069] [MGI Ref ID J:20965]
Wright ME. 1947. Undulated: a new genetic factor in Mus musculus affecting the spine and tail Heredity 1:137-41. [MGI Ref ID J:314]
we relatedBultman SJ; Klebig ML; Michaud EJ; Sweet HO; Davisson MT; Woychik RP. 1994. Molecular analysis of reverse mutations from nonagouti (a) to black-and-tan (a(t)) and white-bellied agouti (Aw) reveals alternative forms of agouti transcripts. Genes Dev 8(4):481-90. [PubMed: 8125260] [MGI Ref ID J:16984]
Candille SI; Raamsdonk CD; Chen C; Kuijper S; Chen-Tsai Y; Russ A; Meijlink F; Barsh GS. 2004. Dorsoventral patterning of the mouse coat by tbx15. PLoS Biol 2(1):E3. [PubMed: 14737183] [MGI Ref ID J:87455]
Dickie MM. 1969. Mutations at the agouti locus in the mouse. J Hered 60(1):20-5. [PubMed: 5798139] [MGI Ref ID J:30922]
Dunn LC. 1928. A Fifth Allelomorph in the Agouti Series of the House Mouse. Proc Natl Acad Sci U S A 14(10):816-9. [PubMed: 16587414] [MGI Ref ID J:15011]
Dunn LC. 1936. Studies on multiple allelomorphic series in the house mouse. I. Description of agouti and albino series of allelomorphs J Genet 33:443-53. [MGI Ref ID J:22600]
Galbraith DB; Wolff GL; Brewer NL. 1980. Hair pigment patterns in different integumental environments of the mouse. Influence of the agouti suppressor (A<s>) mutation on expression of agouti locus alleles. J Hered 71:229-234. [MGI Ref ID J:12033]
Hollander WF. 1973. a<t>/a Mouse News Lett 48:33. [MGI Ref ID J:27528]
Leamy LJ; Hrubant HE. 1971. Effects of alleles at the agouti locus on odontometric traits in the C57BL-6 strain of house mice. Genetics 67(1):87-96. [PubMed: 5556294] [MGI Ref ID J:16571]
Miller MW; Duhl DM; Vrieling H; Cordes SP; Ollmann MM; Winkes BM; Barsh GS. 1993. Cloning of the mouse agouti gene predicts a secreted protein ubiquitously expressed in mice carrying the lethal yellow mutation. Genes Dev 7(3):454-67. [PubMed: 8449404] [MGI Ref ID J:4186]
Poole TW. 1982. The agouti suppressor (As) coat color mutation in mice: developmental effects on the expression of agouti locus alleles. J Exp Zool 220(1):57-64. [PubMed: 7077265] [MGI Ref ID J:6763]
Poole TW; Silvers WK. 1976. The development of regional pigmentation patterns in black and tan (at) mice. J Exp Zool 197(1):115-9. [PubMed: 781177] [MGI Ref ID J:5672]
SILVERS WK. 1958. An experimental approach to action of genes at the agouti locus in the mouse. III. Transplants of newborn Aw-, A-and at-skin to Ay-, Aw-, A-and aa hosts. J Exp Zool 137(1):189-96. [PubMed: 13563791] [MGI Ref ID J:13013]
Sandgren K; Larsson LT; Ekelund M; Robinsson D; Ekblad E. 1998. Functional and morphological examination of ganglionic and aganglionic distal gut from the lethal spotted mouse. Eur J Pediatr Surg 8(4):234-9. [PubMed: 9783148] [MGI Ref ID J:113064]
Silvers WK. 1979. The Coat Colors of Mice; A Model for Mammalian Gene Action and Interaction. In: The Coat Colors of Mice. Springer-Verlag, New York. [MGI Ref ID J:78801]
Summerhayes IC; Franks LM. 1979. Effects of donor age on neoplastic transformation of adult mouse bladder epithelium in vitro. J Natl Cancer Inst 62(4):1017-23. [PubMed: 107359] [MGI Ref ID J:56454]
Vrieling H; Duhl DM; Millar SE; Miller KA; Barsh GS. 1994. Differences in dorsal and ventral pigmentation result from regional expression of the mouse agouti gene. Proc Natl Acad Sci U S A 91(12):5667-71. [PubMed: 8202545] [MGI Ref ID J:18750]
Wolff GL. 1978. Influence of maternal phenotype on metabolic differentiation of agouti locus mutants in the mouse. Genetics 88(3):529-39. [PubMed: 640377] [MGI Ref ID J:5964]
FALCONER DS. 1954. Linkage in the mouse: the sex-linked genes and Rough. Z Indukt Abstamm Vererbungsl 86(2):263-8. [PubMed: 13227156] [MGI Ref ID J:247]
Graff RJ; Simmons D; Meyer J; Martin-Morgan D; Kurtz M. 1986. Abnormal bone production associated with mutant mouse genes pa and we. J Hered 77(2):109-13. [PubMed: 3711636] [MGI Ref ID J:8296]
Hertwig P. 1942. Neue Mutationen und Koppelungsgruppen bei der Hausmaus Z Indukt Abstamm Vererbungsl 80:220-246. [MGI Ref ID J:208]
Sundberg JP (ed.). 1994. . In: Handbook of Mouse Mutations with Skin and Hair Abnormalities: Animal Models and Biomedical Tools. CRC Press, Boca Raton. [MGI Ref ID J:30359]
Currently there no information available for this strain. This may be due to the supply level of this strain.
| Pricing for USA, Canada and Mexico shipping destinations |
|
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
| Pricing for International shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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| Control | ||
|---|---|---|
| Untyped from the colony | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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