Strain Name:

C3HeB/FeJ-Atrnmg-3J/J

Stock Number:

000069

Order this mouse

Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Strain; Spontaneous Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
GenerationN14p
Generation Definitions

Appearance
mahogany
Related Genotype: Atrnmg-3J A/Atrnmg-3J A

agouti
Related Genotype: Atrnmg-3J A/+ A

Description
Attractin (ATRN) deficiencies cause darkened pigmentation, increased locomotor activity, decreased body weight, and vacuolization and myelination defects in the central nervous system.

Agouti protein competes with alpha-melanocyte stimulating hormone (a-MSH) for binding of melanocortin 1 receptor (MC1R), and this in turn signals pigment type switching from eumelanin production to pheomelanin production. ATRN interacts with agouti possibly to facilitate the interaction with MC1R. In mice, ATRN deficiencies result in decreased pheomelanin production causing darkened ears, tail, feet, and coat color which becomes dark reddish brown as these mice age. Thus, the initial Atrn mutation reported by Lane and Green was called mahogany (mg). ATRN deficiencies darken the coloring caused by nonagouti such that these mice are coal black with no white hairs behind the ears or around the perineum and have blacker ears, tail, and feet. ATRN deficiency can suppress the impact on coat color of the Ay allele but not the Mc1re allele. The Atrnmg allele is hypomorphic while the Atrnmg-3J allele results from a deletion that is thought to yield a null mutation. While both alleles cause darkened coat color due to decreased pheomelanin production, the increased severity of the Atrnmg-3J allele is evident in the coat color of mahogany mice carrying the yellow allele of agouti: Ay Atrnmg/A Atrnmg mice are dark brown on the back but yellow on the belly while Ay Atrnmg-3J/A Atrnmg-3J mice are entirely black with dark ears and tail. (He et al., 2001.)

In the central nervous system, alpha melanocortin hormone (a-MSH) interacts with MC4R, and agouti related protein competes with a-MSH for binding to MC4R. These interactions are important for the regulation of body weight. Agouti protein, which is normally expressed only in the skin, can compete with a-MSH for binding to MC4R. Thus, mice carrying the Ay allele which causes systemic expression of Agouti protein are hyperphagic and develop hyperinsulinemia, hyperleptinemia, hyperglycemia, increased linear growth, and obesity. Atrnmg and Atrnmg-3J have each been shown to suppress some or all of these Ay induced characteristics. While the mahogany mutations are generally characterized as recessive, Miller et al. reported that Atrnmg can suppress in a semidominant manner both the coat color and obesity induced by Ay. Dinulescu et al. reported that mice homozygous for Atrnmg on a C57BL/6J congenic background have increased nighttime locomotor activity, a 0.5 degree increase in body temperature, and increased basal metabolic rate, and are hyperphagic. Gunn et al. reported that mice homozygous for Atrnmg-3J on the C3H/HeJ background and mice homozygous for Atrnmg on a C3H/HeJ congenic background also have increased nighttime locomotor activity but that they have normal food intake and decreased body weight associated with decreased adiposity. Mice homozygous for Atrnmg-3J have decreased fat storage and are resistant to weight gain when fed a high fat diet. Atrn mutations do not alter the obesity caused by a null mutation of Mcr4 or transgenic expression of Agouti related protein, nor do they inhibit obesity caused by the tub, Cpefat, Leprob or Leprdb alleles. (Nagle et al., 1999; He et al., 2001; Dinulescu et al., 1998.)

In addition to their coat color changes and metabolic changes, Atrn deficient mice also have been found to have vacuolization in brain tissue. He et al. reported finding 5-40uM vacuoles in both the gray and white matter of mice homozygous for the Atrnmg-3J allele on a segregating background of C3H/HeJ and C57BL/6J. These were found in the brainstem, cerebellar medulla, granular layer of the cerebellum, pons, thalamus, hippocampus, caudate and putamen, somatosensory cortex and spinal cord gray matter, and fewer were found in the primary motor cortex, visual cortex, and white matter tracts of the spinal cord. Transgenic expression of attractin driven by the Beta-actin promoter prevented this phenotype in mice homozygous for Atrnmg-3J but did not cause any additional obvious phenotype. Kuramoto et al. found vacuoles up to 10-20uM in diameter widely distributed in the brainstem, cerebral cortex, cerebellum, and spinal cord of 40 day old mice homozygous for the Atrnmg-3J allele on the C3H/HeJ background. They also described aberrant myelination and a mild tremor (17 Hz) in the adults. Bronson et al. found vacuoles in brain samples of mice homozygous for the Atrnmg, Atrnmg-3J, and Atrn6J alleles as well as Atrnmg/Atrnmg-6J and Atrnmg-3J/Atrnmg-6J heterozygotes. The severity of this phenotype increased with age and varied between strains. By two months of age, vacuoles were found in the granule layer of the cerebellar cortex, deeper layers of the cerebral cortex, and various nuclei of the medulla, midbrain, and thalamus, and by nine months of age vacuoles were found throughout the brain. These vacuoles were generally not found in white matter tracts. Electron microscopy showed vacuoles in the axons, dendrites, and neuronal soma. Myelination defects specific to the central nervous system were found in mice homozygous for the mg-6J allele of Atrn but not other mutant alleles of Atrn. Bronson et al. also described severe tremors and a sprawling gait in Atrnmg-6J/Atrnmg-6J mice on the CAST/Ei background and decreased severity after backcrossing to C3H/SnJ. They also found that Atrnmg-3J/Atrnmg-3J mice are prone to "seizures that begin with a sudden freezing of motion and progress to tonic-clonic movements". They did not find seizures in Atrnmg/Atrnmgmice. In the rat, the zitter allele of attractin has been found to cause hypomyelination and vacuolization in the central nervous system resulting in early-onset tremor. (Kuramoto et al., 2001.)

Control Information

  Control
   000658 C3HeB/FeJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Atrn
004135   C3Sn.CAST-Atrnmg-6J/J
000289   LDJ/LeJ
View Strains carrying other alleles of Atrn     (2 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Atrnmg-3J/Atrnmg-3J

        C3HeB/FeJ-Atrnmg-3J/J
  • pigmentation phenotype
  • abnormal coat/hair pigmentation
    • reduced levels of pheomelanin and 20-30% reductions in eumelanin between 5 and 11 months of age   (MGI Ref ID J:71075)
    • lacks yellow bands in hair   (MGI Ref ID J:71075)
    • darkened coat color
      • mahogany coat   (MGI Ref ID J:96070)
      • yellow pigment is completely absent resulting in a black coat   (MGI Ref ID J:81466)
  • nervous system phenotype
  • abnormal brain morphology   (MGI Ref ID J:80364)
    • brain vacuoles
      • vacuoles in the brain   (MGI Ref ID J:96070)
      • vacuoles in all regions of the CNS by 1 month of age   (MGI Ref ID J:71075)
      • vacuolation becomes exaggerated with increasing age   (MGI Ref ID J:71075)
      • most vacuoles in the gray matter of 6-month old mutants are membrane bound, not enclosed by myelin sheaths, and appear to originate from dendrites or astrocytic processes   (MGI Ref ID J:81466)
  • astrocytosis
    • by 11 to 12 months of age, many brain regions exhibit moderate to severe astrocytosis   (MGI Ref ID J:81466)
  • seizures
    • observed in a few mutants, characterized by a sudden freezing of motion followed by toppling over onto the side and did not progress to tonic-clonic convulsive activity   (MGI Ref ID J:96070)
  • spongiform encephalopathy
    • age-dependent progression of spongiform degeneration   (MGI Ref ID J:81466)
  • growth/size/body phenotype
  • decreased total body fat amount
    • 20-40% reduction in body fat   (MGI Ref ID J:71075)
  • postnatal growth retardation
    • males weigh 10-15% less than controls by 3 months of age   (MGI Ref ID J:71075)
    • female weights are not significantly lower than controls until 4 months of age   (MGI Ref ID J:71075)
  • adipose tissue phenotype
  • decreased total body fat amount
    • 20-40% reduction in body fat   (MGI Ref ID J:71075)
  • behavior/neurological phenotype
  • hyperactivity
    • nocturnal locomotor activity is elevated 20-30%   (MGI Ref ID J:71075)
  • seizures
    • observed in a few mutants, characterized by a sudden freezing of motion followed by toppling over onto the side and did not progress to tonic-clonic convulsive activity   (MGI Ref ID J:96070)
  • tremors   (MGI Ref ID J:96070)
  • muscle phenotype
  • abnormal muscle physiology   (MGI Ref ID J:80364)
  • integument phenotype
  • abnormal coat/hair pigmentation
    • reduced levels of pheomelanin and 20-30% reductions in eumelanin between 5 and 11 months of age   (MGI Ref ID J:71075)
    • lacks yellow bands in hair   (MGI Ref ID J:71075)
    • darkened coat color
      • mahogany coat   (MGI Ref ID J:96070)
      • yellow pigment is completely absent resulting in a black coat   (MGI Ref ID J:81466)

Atrnmg-3J/Atrnmg-3J

        C3HeB/FeJ-Atrnmg-3J
  • nervous system phenotype
  • brain vacuoles
    • mice exhibit widespread vacuolation at 3 moths and extensive vacuolation at 6 months   (MGI Ref ID J:130463)
  • pigmentation phenotype
  • darkened coat color
    • mice exhibit a black coat color   (MGI Ref ID J:130463)
  • integument phenotype
  • darkened coat color
    • mice exhibit a black coat color   (MGI Ref ID J:130463)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Atrnmg-3J related

Dermatology Research
Color and White Spotting Defects

Diabetes and Obesity Research
Obesity With Diabetes
      suppression of Ay-induced obesity

Neurobiology Research
Myelination Defects
Receptor Defects
Tremor Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Atrnmg-3J
Allele Name mahogany 3 Jackson
Allele Type Spontaneous
Common Name(s) mg3J;
Strain of OriginC3HeB/FeJ
Gene Symbol and Name Atrn, attractin
Chromosome 2
Gene Common Name(s) AW558010; DPPT-L; MGCA; expressed sequence AW558010; mKIAA0548; mahogany; mg;
Molecular Note A 5 bp deletion near the end of exon 16 results in a frameshift and premature stop three codons downstream. Western blot analysis of whole brain extracts from homozygous mice shows absence of Atrn protein. [MGI Ref ID J:53417]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Gunn TM; Inui T; Kitada K; Ito S; Wakamatsu K; He L; Bouley DM; Serikawa T; Barsh GS. 2001. Molecular and phenotypic analysis of attractin mutant mice. Genetics 158(4):1683-95. [PubMed: 11514456]  [MGI Ref ID J:71075]

Gunn TM; Miller KA; He L; Hyman RW; Davis RW; Azarani A; Schlossman SF ; Duke-Cohan JS ; Barsh GS. 1999. The mouse mahogany locus encodes a transmembrane form of human attractin. Nature 398(6723):152-6. [PubMed: 10086356]  [MGI Ref ID J:53415]

He L; Gunn TM; Bouley DM; Lu XY; Watson SJ; Schlossman SF; Duke-Cohan JS; Barsh GS. 2001. A biochemical function for attractin in agouti-induced pigmentation and obesity. Nat Genet 27(1):40-7. [PubMed: 11137996]  [MGI Ref ID J:66735]

Atrnmg-3J related

Azouz A; Gunn TM; Duke-Cohan JS. 2007. Juvenile-onset loss of lipid-raft domains in attractin-deficient mice. Exp Cell Res 313(4):761-71. [PubMed: 17196964]  [MGI Ref ID J:119532]

Bronson RT; Donahue LR; Samples R; Kim JH; Naggert JK. 2001. Mice with mutations in the mahogany gene Atrn have cerebral spongiform changes. J Neuropathol Exp Neurol 60(7):724-30. [PubMed: 11444801]  [MGI Ref ID J:96070]

Cota CD; Liu RR; Sumberac TM; Jung S; Vencato D; Millet YH; Gunn TM. 2008. Genetic and phenotypic studies of the dark-like mutant mouse. Genesis 46(10):562-73. [PubMed: 18821597]  [MGI Ref ID J:143333]

Gohma H; Kuramoto T; Matalon R; Surendran S; Tyring S; Kitada K; Sasa M; Serikawa T. 2007. Absence-like and tonic seizures in aspartoacylase/attractin double-mutant mice. Exp Anim 56(2):161-5. [PubMed: 17460362]  [MGI Ref ID J:122809]

Gunn TM; Inui T; Kitada K; Ito S; Wakamatsu K; He L; Bouley DM; Serikawa T; Barsh GS. 2001. Molecular and phenotypic analysis of attractin mutant mice. Genetics 158(4):1683-95. [PubMed: 11514456]  [MGI Ref ID J:71075]

Gunn TM; Miller KA; He L; Hyman RW; Davis RW; Azarani A; Schlossman SF ; Duke-Cohan JS ; Barsh GS. 1999. The mouse mahogany locus encodes a transmembrane form of human attractin. Nature 398(6723):152-6. [PubMed: 10086356]  [MGI Ref ID J:53415]

He L; Gunn TM; Bouley DM; Lu XY; Watson SJ; Schlossman SF; Duke-Cohan JS; Barsh GS. 2001. A biochemical function for attractin in agouti-induced pigmentation and obesity. Nat Genet 27(1):40-7. [PubMed: 11137996]  [MGI Ref ID J:66735]

He L; Lu XY; Jolly AF; Eldridge AG; Watson SJ; Jackson PK; Barsh GS; Gunn TM. 2003. Spongiform degeneration in mahoganoid mutant mice. Science 299(5607):710-2. [PubMed: 12560552]  [MGI Ref ID J:81466]

Kuramoto T; Kitada K; Inui T; Sasaki Y; Ito K; Hase T; Kawagachi S; Ogawa Y; Nakao K; Barsh GS; Nagao M; Ushijima T; Serikawa T. 2001. Attractin/mahogany/zitter plays a critical role in myelination of the central nervous system. Proc Natl Acad Sci U S A 98(2):559-64. [PubMed: 11209055]  [MGI Ref ID J:80364]

Miller KA; Gunn TM; Carrasquillo MM; Lamoreux ML; Galbraith DB ; Barsh GS. 1997. Genetic studies of the mouse mutations mahogany and mahoganoid. Genetics 146(4):1407-15. [PubMed: 9258683]  [MGI Ref ID J:41964]

Nagle DL; McGrail SH; Vitale J; Woolf EA; Dussault BJ Jr; DiRocco L; Holmgren L; Montagno J; Bork P; Huszar D; Fairchild-Huntress V; Ge P; Keilty J; Ebeling C; Baldini L; Gilchrist J; Burn P; Carlson GA; Moore KJ. 1999. The mahogany protein is a receptor involved in suppression of obesity. Nature 398(6723):148-52. [PubMed: 10086355]  [MGI Ref ID J:53417]

Silvers WK. 1979. The Coat Colors of Mice; A Model for Mammalian Gene Action and Interaction. In: The Coat Colors of Mice. Springer-Verlag, New York.  [MGI Ref ID J:78801]

Sun K; Johnson BS; Gunn TM. 2007. Mitochondrial dysfunction precedes neurodegeneration in mahogunin (Mgrn1) mutant mice. Neurobiol Aging 28(12):1840-52. [PubMed: 17720281]  [MGI Ref ID J:129981]

The Jackson Laboratory Office of Genetic Resources. 1973. Registry of Remutation at The Jackson Laboratory MGI Direct Data Submission :.  [MGI Ref ID J:79827]

Walker WP; Aradhya S; Hu CL; Shen S; Zhang W; Azarani A; Lu X; Barsh GS; Gunn TM. 2007. Genetic analysis of attractin homologs. Genesis 45(12):744-56. [PubMed: 18064672]  [MGI Ref ID J:130463]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3300.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $4290.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000658 C3HeB/FeJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.6)