Strain Name: |
JGBF/LeTyJ |
|---|---|
Stock Number: |
000072 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Vps33a; Vps33abf; jg; |
Product Information
Strain Details
Type Inbred Strain Additional information on Inbred Strains. Type JAX® GEMM® Strain - Spontaneous Mutation Additional information on JAX® GEMM® Strains. Species laboratory mouse Generation F36 Strain Description
Mice homozygous for the recessive buff (Vps33abf) mutation on a non-agouti (a/a) background have a lightened coat color that has been described as khaki. On an agouti background, Vps33abf homozygotes have dark ears, opaque eyes, and the belly is lighter in color than is the rest of the coat. Although no change in eye color is outwardly evident, electron microscopy shows fewer and smaller melanosomes than normal in the retinal pigment epithelium and choroid. These mutants have a platelet-storage pool defect evidenced by an increased bleeding time of 9.7 minutes on average, a reduction in the number of platelet dense granules, and slightly decreased collagen-mediated platelet aggregation, secretion of dense-granule ATP and secretion of seratonin. Hakansson and Lundin found increased activity of the lysosomal glycosidases beta- galactosidase, beta-glucuronidase, and N-acetyl-beta-hexosaminidase in kidney cell lysates. Suzuki et al. found no significant difference in kidney beta- galactosidase and beta-glucuronidase activity, no significant decrease in beta-glucuronidase secretion into the urine, but a 53% reduction in the secretion of beta-galactosidase into the urine. (Dickie, 1964; Davisson, 1977; Hakansson and Lundin, 1977; Suzuki et al., 2003.)Mice homozygous for jg have shortened tails with a normal tapering tip. There is variation in expressivity. The tail is often kinked and other parts of the spine can also be affected. Testes are much smaller than normal and homozygous males are likely sterile. Ovaries are slightly smaller than normal and litter sizes from homozygous females are smaller than normal. Viability to wean age is reduced in homozygotes impacting males more than females. (Green, 1964.)
Related Disease (OMIM) Terms |
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Vps33abf | ||
|---|---|---|---|
| Allele Name | buff | ||
| Common Name(s) | bf; | ||
| Strain of Origin | C57BL/6J | ||
| Gene Symbol and Name | Vps33a, vacuolar protein sorting 33A (yeast) | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | 3830421M04Rik; AI503300; AW048546; AW554476; FLJ22395; FLJ23187; RIKEN cDNA 3830421M04 gene; bf; buff; expressed sequence AI503300; expressed sequence AW048546; expressed sequence AW554476; r-Vps33a; | ||
| Molecular Note | The buff mutation arose spontaneously in the C57BL/6J strain. The mutation was identified as a T to G transversion that causes a missense substitution of glutamic acid for aspartic acid at codon 251. [MGI Ref ID J:81830] | ||
| Allele Symbol | jg | ||
| Allele Name | jagged tail | ||
| Strain of Origin | C3H/HeJ | ||
Colony Maintenance
| Breeding & Husbandry | Homozygous jg/jg usually dies at birth. |
|---|
Related Strains
Strains carrying Vps33abf allele
000520 C57BL/6J-Vps33abf/J 006938 C57BL/6J-Vps33abf/JLlp 000260 JGBF/LeJ View Strains carrying Vps33abf (3 strains)
000260 JGBF/LeJ
Research Applications
This mouse can be used to support research in many areas including:Vps33abf related
jg relatedDermatology Research
Color and White Spotting Defects
Developmental Biology Research
Skeletal Defects
Reproductive Biology Research
Developmental Defects Affecting Gonads
Fertility Defects
References
Additional ReferencesPrice and Supply Information
| Strain Name: | JGBF/LeTyJ |
| Stock Number: | 000072 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery of Strains Needing Progeny Testing. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two untested males and two untested females (two pairs) will be recovered, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the overall recovery time to approximately 25 weeks. However, all pups recovered will be sent. Progeny testing is required to identify the genotype of mice of this strain, as a genotyping assay is not available. This type of testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. We can perform the progeny testing for you as a service or we can ship all recovered animals (at least two untested pairs) to you for progeny testing at your facility. If you perform the progeny testing, there is NO guarantee that a carrier will be identified. If we perform progeny testing as a service, additional breeding time will be required. In this case, when a male and female (one pair) are identified that carry the mutation, they and their offspring will be shipped. Delivery time for strains requiring progeny testing often exceeds 25 weeks and may take 12 months or more due to the difficulties in breeding some strains. The progeny testing cost is in addition to the recovery cost and is based on the number of boxes used and the time taken to produce the mice identified as carrying the mutation. Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Please contact Customer Service for more information on the cost of progeny testing for a strain: Tel: 1-800-422-6423 or 1-207-288-5845. Cryorecovery to establish a Dedicated Supply for greater quantities of mice |
| Licensing | See General Terms and Conditions below |
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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