Strain Name:

129S1/Sv-Oca2+ Tyr+ KitlSl-J/J

Stock Number:

000090

Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names 129S1/Sv-p+ Tyr+ KitlSl-J/J    (Changed: 11-FEB-08 )
129S1/Sv-p+Tyr+KitlSl-J    (Changed: 03-FEB-06 )
129/Sv-SlJ/+ c+ p+/c+ p+    (Changed: 15-DEC-04 )
Type Mutant Strain; Spontaneous Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
GenerationF12p

Description
The multiple steel mutations (KitlSl) behave in a semidominant fashion and cause deficiencies in pigment cells, germ cells, and blood cells paralleling those caused by the Kit locus mutations (dominant spotting alleles). Most of the alleles at steel locus cause severe anemia in utero and death by 15 to 16 days of gestation in homozygous mutant mice. However, compounds of two steel mutants (e.g. KitlSl/KitlSl-d) are viable, black-eyed white, are usually sterile in one or both sexes, and have severe macrocytic anemia. Heterozygous steel mice have a diluted coat color with a small amount of white spotting, are viable and fertile, and may have a slight macrocytic anemia. Primordial germ cells are absent in the nonviable steel homozygotes and severely reduced in steel heterozygotes. Mast cells are virtually absent in skin and other tissues of steel mutant mice. Tumors tend to develop in germ-cell-deficient ovaries with advancing age. The steel-J mutation (KitlSl-J) on this 129S1 genetic background increases the incidence of testicular teratomas to approximately 10%.

Control Information

  Control
   Wild-type from the colony
   002448 129S1/SvImJ
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying   Oca2+     (4 strains)

Strains carrying   Tyr+ allele
005445   A.B6 Tyr+-Cybanmf333/J
005012   A.B6 Tyr+-Myo5ad-l31J/J
002565   A.B6-Tyr+/J
000822   B6 x 129S1/SvEi Oca2+ Tyr+-Vsx2or-J/J
000899   C.B6-Tyr+ Hbbs/J
000494   J.Cg-Oca2+ Tyr+ Lystbg/J
002281   NFS.C58-Tyr+/J
004304   NOD.CBALs-Tyr+/LtJ
View Strains carrying   Tyr+     (8 strains)

Strains carrying other alleles of Kitl
002993   B6.Cg-KitlSl-18H/EiJ
008656   B6.Cg-KitlSl-gb/MbeJ
000124   B6.Cg-KitlSl Krt71Ca/J
000160   B6.D2-KitlSl-d/J
000291   C3FeLe.Cg-a/a Hm KitlSl Krt71Ca-J/J
001380   C3Sn.Cg-KitlSl-con/J
003252   C57BL/6J-KitlSl-20J/J
000979   STOCK KitlSl-16J/J
000161   WB.D2-KitlSl-d/J
000693   WC/ReJ KitlSl/J
100401   WCB6F1/J KitlSl KitlSl-d
View Strains carrying other alleles of Kitl     (11 strains)

View Strains carrying other alleles of Oca2     (19 strains)

View Strains carrying other alleles of Tyr     (38 strains)

Additional Web Information

JAX® NOTES, February 2001; 481. Mgf Gene Name Changes to Kitl.
New 129 Nomenclature Bulletin

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

KitlSl-J/Kitl+

        C3HeB/FeHu
  • pigmentation phenotype
  • diluted coat color (MGI Ref ID J:145144)
    • lightened coat color compared to normal and the belly is very light
  • white spotting (MGI Ref ID J:145144)
    • the feet and tail tip are white
    • a white dot on the head is characteristic
    • belly spot (MGI Ref ID J:145144)
      • a mid-ventral white streak becomes more apparent with age
  • skin/coat/nails phenotype
  • diluted coat color (MGI Ref ID J:145144)
    • lightened coat color compared to normal and the belly is very light
  • white spotting (MGI Ref ID J:145144)
    • the feet and tail tip are white
    • a white dot on the head is characteristic
    • belly spot (MGI Ref ID J:145144)
      • a mid-ventral white streak becomes more apparent with age

KitlSl-J/Kitl+

        129
  • tumorigenesis
  • testicular teratoma (MGI Ref ID J:50508)
    • tumor incidence is markedly increased in the presence of this allele
    • in the presence of this allele, appreciable differences are seen in the incidence of tumors among 129 sublines

KitlSl-J/KitlSl-J

        C3HeB/FeHu
  • lethality-prenatal/perinatal
  • perinatal lethality (MGI Ref ID J:145144)
    • mice die from severe anemia
  • hematopoietic system phenotype
  • anemia (MGI Ref ID J:145144)
  • endocrine/exocrine gland phenotype
  • abnormal seminiferous tubule morphology (MGI Ref ID J:145144)
    • contain only Sertoli cells
  • absent ovarian follicles (MGI Ref ID J:145144)
  • reproductive system phenotype
  • abnormal seminiferous tubule morphology (MGI Ref ID J:145144)
    • contain only Sertoli cells
  • absent germ cells (MGI Ref ID J:145144)
    • in both females and males
  • absent ovarian follicles (MGI Ref ID J:145144)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

KitlSl-J related

Cancer Research
Growth Factors/Receptors/Cytokines
Increased Tumor Incidence
      Gonadal Tumors: ovarian and testicular

Dermatology Research
Color and White Spotting Defects

Developmental Biology Research
Neural Crest Defects

Endocrine Deficiency Research
Bone/Bone Marrow Defects
Gonad Defects
Hypothalamus/Pituitary Defects
Skin Defects

Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Immunodeficiency
      Mast Cell Deficiency

Neurobiology Research
Vestibular and Hearing Defects

Reproductive Biology Research
Developmental Defects Affecting Gonads
      germ cell deficient
Fertility Defects
Gonadal Tumors
      ovarian and testicular

Research Tools
Immunology and Inflammation Research
      Mast Cell Deficiency

Sensorineural Research
Vestibular and Hearing Defects

Tyr+ related

Dermatology Research
Color and White Spotting Defects
      oculocutaneous albinism, type I

Mouse/Human Gene Homologs
albinism, tyrosine negative
      wildtype

Genes & Alleles

Gene & Allele Information

 
Allele Symbol KitlSl-J
Allele Name steel Jackson
Allele Type Spontaneous
Common Name(s) MgfSl-J; SlJ;
Strain of OriginC3HeB/FeHu
Gene Symbol and Name Kitl, kit ligand
Chromosome 10
Gene Common Name(s) Clo; Con; DKFZp686F2250; FPH2; Gb; KL-1; MGF; Mgf; SCF; SF; SHEP7; SLF; Sl; Steel; Steel factor; cloud gray; contrasted; grizzle-belly; mast cell growth factor; steel; stem cell factor;
Molecular Note Southern blot analysis and pulsed field gel electrophoresis revealed that this allele comprises a 650kb deletion. [MGI Ref ID J:31566]
 
Allele Symbol Oca2+
Allele Name wild type
Allele Type Not Specified
Gene Symbol and Name Oca2, oculocutaneous albinism II
Chromosome 7
Gene Common Name(s) BEY; BEY1; BEY2; BOCA; D15S12; D7H15S12; D7Icr28RN; D7Nic1; DNA segment, Chr 7, Institute for Cancer Research 28RN; DNA segment, Chr 7, Nicholls 1; DNA segment, Chr 7, human D15S12; EYCL; EYCL2; EYCL3; HCL3; P; PED; SHEP1; p; pink-eyed dilution;
 
Allele Symbol Tyr+
Allele Name wild type
Allele Type Not Applicable
Gene Symbol and Name Tyr, tyrosinase
Chromosome 7
Gene Common Name(s) C; OCA1A; OCAIA; SHEP3; albino; c; skc35; skin/coat color 35;

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Genotyping resources and troubleshooting

References

References

Additional References

Arguello F; Furlanetto RW; Baggs RB; Graves BT; Harwell SE; Cohen HJ; Frantz CN. 1992. Incidence and distribution of experimental metastases in mutant mice with defective organ microenvironments (genotypes Sl/Sld and W/Wv). Cancer Res 52(8):2304-9. [PubMed: 1559233]  [MGI Ref ID J:468]

Hayashi C; Sonoda T; Nakano T; Nakayama H; Kitamura Y. 1985. Mast-cell precursors in the skin of mouse embryos and their deficiency in embryos of Sl/Sld genotype. Dev Biol 109(1):234-41. [PubMed: 3987963]  [MGI Ref ID J:7810]

Huang E; Nocka K; Beier DR; Chu TY; Buck J; Lahm HW; Wellner D; Leder P; Besmer P. 1990. The hematopoietic growth factor KL is encoded by the Sl locus and is the ligand of the c-kit receptor, the gene product of the W locus. Cell 63(1):225-33. [PubMed: 1698557]  [MGI Ref ID J:10751]

Marians RC; Ng L; Blair HC; Unger P; Graves PN; Davies TF. 2002. Defining thyrotropin-dependent and -independent steps of thyroid hormone synthesis by using thyrotropin receptor-null mice. Proc Natl Acad Sci U S A 99(24):15776-81. [PubMed: 12432094]  [MGI Ref ID J:80513]

Murphy ED. 1977. Effects of mutant steel alleles on leukemogenesis and life-span in the mouse. J Natl Cancer Inst 58(1):107-10. [PubMed: 319242]  [MGI Ref ID J:5758]

Schrott A; Egg G; Spoendlin H. 1988. Intermediate filaments in the cochleas of normal and mutant (w/wv, sl/sld) mice. Arch Otorhinolaryngol 245(4):250-4. [PubMed: 2460075]  [MGI Ref ID J:9423]

Schrott A; Spoendlin H. 1987. Pigment anomaly-associated inner ear deafness. Acta Otolaryngol (Stockh) 103(5-6):451-7. [PubMed: 3618172]  [MGI Ref ID J:8813]

Tang SH; Silva FJ; Tsark WM; Mann JR. 2002. A cre/loxP-deleter transgenic line in mouse strain 129S1/SvImJ. Genesis 32(3):199-202. [PubMed: 11892008]  [MGI Ref ID J:75826]

Wolf NS. 1978. Dissecting the hematopoietic microenvironment. II. The kinetics of the erythron of the S1/S1d mouse and the dual nature of its anemia. Cell Tissue Kinet 11(4):325-34. [PubMed: 688326]  [MGI Ref ID J:6031]

Zsebo KM; Williams DA; Geissler EN; Broudy VC; Martin FH; Atkins HL; Hsu RY; Birkett NC; Okino KH; Murdock DC; Jacobsen FW; Langley KE; Smith KA; Takeishi T; Cattanach BM; Galli SJ; Suggs SV. 1990. Stem cell factor is encoded at the Sl locus of the mouse and is the ligand for the c-kit tyrosine kinase receptor. Cell 63(1):213-24. [PubMed: 1698556]  [MGI Ref ID J:10750]

KitlSl-J related

Bedell MA; Cleveland LS; O'Sullivan TN; Copeland NG; Jenkins NA. 1996. Deletion and interallelic complementation analysis of Steel mutant mice. Genetics 142(3):935-44. [PubMed: 8849899]  [MGI Ref ID J:31566]

Hummel KP. 1955. Dominant Sl-type mutation (to be Sl-J) Mouse News Lett 12:29-30.  [MGI Ref ID J:145144]

Hummel KP. 1955. Dominant Sl-type mutation is Sl-J Mouse News Lett 13:38.  [MGI Ref ID J:145145]

Lam MY; Nadeau JH. 2003. Genetic control of susceptibility to spontaneous testicular germ cell tumors in mice. APMIS 111(1):184-90; discussion 191. [PubMed: 12752260]  [MGI Ref ID J:82965]

Lam MY; Youngren KK; Nadeau JH. 2004. Enhancers and suppressors of testicular cancer susceptibility in single- and double-mutant mice. Genetics 166(2):925-33. [PubMed: 15020477]  [MGI Ref ID J:89604]

Matin A; Collin GB; Varnum DS; Nadeau JH. 1998. Testicular teratocarcinogenesis in mice--a review. APMIS 106(1):174-82. [PubMed: 9524576]  [MGI Ref ID J:46683]

Stevens LC. 1973. A new inbred subline of mice (129-terSv) with a high incidence of spontaneous congenital testicular teratomas. J Natl Cancer Inst 50(1):235-42. [PubMed: 4692863]  [MGI Ref ID J:29502]

Stevens LC; Mackensen JA. 1961. Genetic and environmental influences on teratocarcinogenesis in mice J Natl Cancer Inst 27:443-453.  [MGI Ref ID J:50508]

Oca2+ related

Mouse Genome Informatics (MGI). 2006. Information obtained from The RIKEN BioResource Center :.  [MGI Ref ID J:104881]

Tyr+ related

Lossie AC; Nakamura H; Thomas SE; Justice MJ. 2005. Mutation of l7Rn3 shows that Odz4 is required for mouse gastrulation. Genetics 169(1):285-99. [PubMed: 15489520]  [MGI Ref ID J:96673]

Miller DA; Dev VG; Tantravahi R; Miller OJ; Schiffman MB; Yates RA; Gluecksohn-Waelsch S. 1974. Cytological detection of the c-25H deletion involving the albino (c) locus on chromosome 7 in the mouse. Genetics 78(3):905-10. [PubMed: 4141683]  [MGI Ref ID J:5520]

Sweet HO. 1987. Acromelanic (c<a>) Mouse News Lett 78:56.  [MGI Ref ID J:14994]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Price (US dollars $)
Cryorecovery Fee $1900.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Price (US dollars $)
Cryorecovery Fee $2470.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Additional Supply Details

Supply Details

Standard SupplyCryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

  • Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

  Control
   Wild-type from the colony
   002448 129S1/SvImJ
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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