Strain Name:

B6.C3-Zbtb16Lu/J

Stock Number:

000100

Availability:

Repository-Cryopreserved

Description

Strain Information

Former Names B6.C3-Zfp145Lu/J    (Changed: 15-DEC-04 )
B6.C3-lu/J    (Changed: 15-DEC-04 )
Type Congenic; Mutant Strain; Spontaneous Mutation;
Additional information on Genetically Engineered Mutant Mice.
Specieslaboratory mouse
Background Strain C57BL/6J
Donor Strain C3H/He
GenerationN37p

Description
Mice homozygous for the luxoid mutation exhibit preaxial polydactly or oligodactly of the hindfeet, preaxial polydactly of the forefeet, tail kinks, tibial hemimelia and occasionally radial hemimelia. Heterozygotes exhibit preaxial polydactly or hyperphalangy of the hindfeet. An increased number of vertebra, ribs and sternebrae are found in homozygotes and to a lesser extent in heterozygotes (Forsthoefel, 1958). Male mice are infertile with a complete lack of spermatogonia after six weeks (Johnson et al., 1971). By eight months, many seminiferous tubules are agametic, lacking one or more germ cell generations (Buaas et al., 2004). Histological and FACS analysis demonstrate that luxoid homozygotes have progressive germ-cell loss starting as early as eight days (Buaas et al., 2004). This mutant mouse strain may be useful in studies related to male infertility or contraception, limb or skeletal abnormalities, and stem cell research.

Development
The luxoid mutation arose spontaneously in the C3H/He inbred strain at The Jackson Laboratory (Green 1955). The mutation was backcrossed to C57BL/10Gn for 69 generations and then backcrossed for 35+ generations to C57BL/6J before embryos were cryopreserved.

Control Information

  Control
   Untyped from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

Zbtb16Lu/Zbtb16Lu

        B6.Cg-Zbtb16Lu
  • endocrine/exocrine gland phenotype
  • small testis (MGI Ref ID J:89934)
    • observed in 8 month old mice; suggested to be due to loss of germ cells
  • reproductive system phenotype
  • decreased male germ cell number (MGI Ref ID J:89934)
    • many seminiferous tubules of older mice were agametic, containing only Sertoli cells; other tubules lacked one or more germ cell generations
    • azoospermia (MGI Ref ID J:89934)
      • observed in 8 month old mice
  • small testis (MGI Ref ID J:89934)
    • observed in 8 month old mice; suggested to be due to loss of germ cells

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Zbtb16Lu/Zbtb16+

        C3H/He
  • limbs/digits/tail phenotype
  • polyphalangy (MGI Ref ID J:13069)
    • incomplete penetrance on hind limb only; the first toe is the usually the only one affected, with either an elongated second phalanx or having three phalanges
  • skeleton phenotype
  • abnormal sternebra morphology (MGI Ref ID J:13069)
    • increased number of sternebrae
  • increased presacral vertebrae number (MGI Ref ID J:13069)
  • increased rib number (MGI Ref ID J:13069)
    • higher frequency of mice with 14 ribs instead of 13
  • polyphalangy (MGI Ref ID J:13069)
    • incomplete penetrance on hind limb only; the first toe is the usually the only one affected, with either an elongated second phalanx or having three phalanges

Zbtb16Lu/Zbtb16+

        B10.C3-Zbtb16Lu
  • limbs/digits/tail phenotype
  • polydactyly (MGI Ref ID J:13069)
    • preaxial polydactyly; incomplete penetrance on hind limb only; up to two extra toes are seen with variable degrees of development
  • polyphalangy (MGI Ref ID J:13069)
    • incomplete penetrance on hind limb only; the first toe is the usually the only one affected, with either an elongated second phalanx or having three phalanges
  • skeleton phenotype
  • abnormal sternebra morphology (MGI Ref ID J:13069)
    • increased number of sternebrae
  • increased presacral vertebrae number (MGI Ref ID J:13069)
  • increased rib number (MGI Ref ID J:13069)
    • higher frequency of mice with 14 ribs instead of 13
  • polyphalangy (MGI Ref ID J:13069)
    • incomplete penetrance on hind limb only; the first toe is the usually the only one affected, with either an elongated second phalanx or having three phalanges

Zbtb16Lu/Zbtb16Lu

        C3H/He
  • limbs/digits/tail phenotype
  • abnormal tarsus morphology (MGI Ref ID J:13069)
  • bowed fibula (MGI Ref ID J:13069)
    • curved
  • brachyphalangia (MGI Ref ID J:13069)
    • occasional preaxial reduction; hallux is reduced or absent
  • kinked tail (MGI Ref ID J:13069)
  • long fibula (MGI Ref ID J:13069)
  • polydactyly (MGI Ref ID J:13069)
    • preaxial polydactyly; incomplete penetrance; less frequent than on the C57BL/10 background; often an extra preaxial digit is present; up to two extra toes are seen with variable degrees of development; the second or third digit may be split
  • polyphalangy (MGI Ref ID J:13069)
    • incomplete penetrance; the first toe usually has three phalanges
  • short tibia (MGI Ref ID J:13069)
    • results in the curvature of the fibula and the knee and heel are brought close together
  • skeleton phenotype
  • abnormal sternebra morphology (MGI Ref ID J:13069)
    • increased number of sternebrae
  • abnormal tarsus morphology (MGI Ref ID J:13069)
  • bowed fibula (MGI Ref ID J:13069)
    • curved
  • brachyphalangia (MGI Ref ID J:13069)
    • occasional preaxial reduction; hallux is reduced or absent
  • increased presacral vertebrae number (MGI Ref ID J:13069)
  • increased rib number (MGI Ref ID J:13069)
    • higher frequency of mice with 14 ribs instead of 13
  • long fibula (MGI Ref ID J:13069)
  • polyphalangy (MGI Ref ID J:13069)
    • incomplete penetrance; the first toe usually has three phalanges
  • short tibia (MGI Ref ID J:13069)
    • results in the curvature of the fibula and the knee and heel are brought close together

Zbtb16Lu/Zbtb16Lu

        B10.C3-Zbtb16Lu
  • limbs/digits/tail phenotype
  • abnormal tarsus morphology (MGI Ref ID J:13069)
  • kinked tail (MGI Ref ID J:13069)
  • long fibula (MGI Ref ID J:13069)
  • polydactyly (MGI Ref ID J:13069)
    • preaxial polydactyly; incomplete penetrance; often an extra preaxial digit is present; up to two extra toes are seen with variable degrees of development; the second or third digit may be split
  • polyphalangy (MGI Ref ID J:13069)
    • incomplete penetrance; the first toe usually has three phalanges
  • short tibia (MGI Ref ID J:13069)
    • less severe than C3H background; results in increased length of the fibula and the shank and foot are not held in the normal position
  • skeleton phenotype
  • abnormal sternebra morphology (MGI Ref ID J:13069)
    • increased number of sternebrae
  • abnormal tarsus morphology (MGI Ref ID J:13069)
  • increased presacral vertebrae number (MGI Ref ID J:13069)
  • increased rib number (MGI Ref ID J:13069)
    • higher frequency of mice with 14 ribs instead of 13
  • long fibula (MGI Ref ID J:13069)
  • polyphalangy (MGI Ref ID J:13069)
    • incomplete penetrance; the first toe usually has three phalanges
  • short tibia (MGI Ref ID J:13069)
    • less severe than C3H background; results in increased length of the fibula and the shank and foot are not held in the normal position

Zbtb16Lu/Zbtb16Lu

        Background Not Specified
  • reproductive system phenotype
  • azoospermia (MGI Ref ID J:5206)
    • lack of spermatogonia after 6 weeks of age; production of spermatogonia is normal at birth, then a rapid loss of germline cells occurs
  • male infertility (MGI Ref ID J:5206)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Zbtb16Lu related

Developmental Biology Research
Skeletal Defects

Reproductive Biology Research
Developmental Defects Affecting Gonads (males only)
Fertility Defects (males only)

Genes & Alleles

Gene & Allele Information

Allele Symbol Zbtb16Lu
Allele Name luxoid
Allele Type Spontaneous
Common Name(s) Green's luxoid; lu;
Strain of OriginC3H/He
Gene Symbol and Name Zbtb16, zinc finger and BTB domain containing 16
Chromosome 9
Gene Common Name(s) AI467657; Green's luxoid; Lx; PLZF; ZNF145; Zfp145; expressed sequence AI467657; lu; luxoid; zinc finger protein 145;
Molecular Note This spontaneous mutation arose in 1950 at the Ohio State University. The molecular mutation responsible for the phenotype is a C-to-T transition mutation in codon 234 that is predicted to convert an arginine residue to a nonsense codon. [MGI Ref ID J:89934]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Buaas FW; Kirsh AL; Sharma M; McLean DJ; Morris JL; Griswold MD; de Rooij DG; Braun RE. 2004. Plzf is required in adult male germ cells for stem cell self-renewal. Nat Genet 36(6):647-52. [PubMed: 15156142]  [MGI Ref ID J:89934]

Burda DJ; Center EM. 1969. Development of luxoid (lu) skeletal defects in vitro. J Embryol Exp Morphol 21(2):347-60. [PubMed: 5822891]  [MGI Ref ID J:5126]

Center EM; Hunter RL; Dodge AH. 1967. Effects of the luxoid gene (lu) on liver esterase isozymes of the mouse. Genetics 55(2):349-58. [PubMed: 6067640]  [MGI Ref ID J:109970]

Johnson DR; Hunt DM. 1971. Hop-sterile, a mutant gene affecting sperm tail development in the mouse. J Embryol Exp Morphol 25(2):223-36. [PubMed: 5088020]  [MGI Ref ID J:5206]

Additional References

Zbtb16Lu related

Dagg CP. 1967. Combined action of fluorouracil and two mutant genes on limb development in the mouse. J Exp Zool 164(3):479-89. [PubMed: 6068186]  [MGI Ref ID J:5039]

Forsthoefel PF. 1959. The embryological development of the skeletal effects of the luxoid gene in the mouse, including its interactions with the luxate gene. J Morphol 104:89-141.  [MGI Ref ID J:13095]

Forsthoefel PF. 1958. The skeletal effects of the luxoid gene in the mouse, including its interactions with the luxate gene. J Morphol 102:247-287.  [MGI Ref ID J:13093]

Green MC. 1955. Luxoid, a new hereditary leg and foot abnormality in the house mouse. J Hered 46:91-99.  [MGI Ref ID J:13069]

Kobozieff N; Pomriaskinsky-Kobozieff NA; Gemahling E. 1962. Hemimelie chez la souris. II. Etude morphologique des homozygotes atteints de differentes anomalies du squelette. D. Squelette axial Rec Med Vet 138:671-86.  [MGI Ref ID J:14899]

Savage AK; Constantinides MG; Han J; Picard D; Martin E; Li B; Lantz O; Bendelac A. 2008. The transcription factor PLZF directs the effector program of the NKT cell lineage. Immunity 29(3):391-403. [PubMed: 18703361]  [MGI Ref ID J:139690]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Price*
Cryorecovery Fee $1900.00
*Price(s) in US dollars ($)

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Price*
Cryorecovery Fee $2470.00
*Price(s) in US dollars ($)

Additional Supply Details

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery of Strains Needing Progeny Testing.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two untested males and two untested females (two pairs) will be recovered, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the overall recovery time to approximately 25 weeks. However, all pups recovered will be sent.

    Progeny testing is required to identify the genotype of mice of this strain, as a genotyping assay is not available. This type of testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. We can perform the progeny testing for you as a service or we can ship all recovered animals (at least two untested pairs) to you for progeny testing at your facility. If you perform the progeny testing, there is NO guarantee that a carrier will be identified. If we perform progeny testing as a service, additional breeding time will be required. In this case, when a male and female (one pair) are identified that carry the mutation, they and their offspring will be shipped. Delivery time for strains requiring progeny testing often exceeds 25 weeks and may take 12 months or more due to the difficulties in breeding some strains. The progeny testing cost is in addition to the recovery cost and is based on the number of boxes used and the time taken to produce the mice identified as carrying the mutation. Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Please contact Customer Service for more information on the cost of progeny testing for a strain: Tel: 1-800-422-6423 or 1-207-288-5845.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

  Control
   Untyped from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Fax: 207.288.6150
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fax:207-288-6655

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