Strain Name:

STOCK Myo15sh2/J

Stock Number:

000109

Order this mouse

Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Radiation Induced Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
GenerationF28p+F1p
Generation Definitions

Appearance
agouti, spins
Related Genotype: A/A Myo15sh2/Myo15sh2

agouti, unaffected
Related Genotype: A/A Myo15sh2/+

Description
Mice homozygous for the shaker 2 spontaneous mutation (Myo15sh2) are viable and fertile, showing only a slight reduction in both compared to wildtype mice. Homozygous mutant mice display a phenotype very similar to the behavior and pathology to shaker-1 (Myo7ash1) with the exception that the abnormalities are observed a little earlier in shaker 2 mice. Homozygotes appear to be deaf from the beginning, and the saccular macula is abnormal at birth. The stria vascularis appears normal by light microscopy at 2 weeks, but begins to show degenerative changes shortly thereafter. Many of the cells contain electron-dense inclusions filled with a fine granular material. At 2.5 months, the type I hair cells of the cristae ampullares and maculae utriculi contain rod-shaped inclusion bodies composed of actin filaments. The visible phenotype of the shaker-2 Jackson (Myo15sh2-J, Stock No. 002048) does not differ from that of the original shaker 2.

Control Information

  Control
   Heterozygote from the colony
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Myo15
002048   B6 x C57BLKS-Dock7m Leprdb Myo15sh2-J/J
003304   STOCK Rb(16.17)7Bnr-Myo15sh2-2J/J
View Strains carrying other alleles of Myo15     (2 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Deafness, Autosomal Recessive 3; DFNB3   (MYO15A)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Myo15sh2/Myo15sh2

        Mixed stock
  • hearing/vestibular/ear phenotype
  • abnormal cochlear outer hair cell morphology
    • most outer hair cells exhibit a non-convex region on their apical circumference unlike wild-type cells   (MGI Ref ID J:158897)
    • outer hair cells exhibit a slight flattening of their neural side compared to in wild-type mice   (MGI Ref ID J:158897)
    • short outer hair cell stereocilia   (MGI Ref ID J:158897)
      • hair cell stereocilia are shorter than in wild-type mice   (MGI Ref ID J:132633)
  • nervous system phenotype
  • abnormal cochlear outer hair cell morphology
    • most outer hair cells exhibit a non-convex region on their apical circumference unlike wild-type cells   (MGI Ref ID J:158897)
    • outer hair cells exhibit a slight flattening of their neural side compared to in wild-type mice   (MGI Ref ID J:158897)
    • short outer hair cell stereocilia   (MGI Ref ID J:158897)
      • hair cell stereocilia are shorter than in wild-type mice   (MGI Ref ID J:132633)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Myo15sh2 related

Neurobiology Research
Hearing Defects
Tremor Defects
Vestibular Defects

Sensorineural Research
Hearing Defects
Vestibular Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Myo15sh2
Allele Name shaker 2
Allele Type Radiation induced
Common Name(s) sh2;
Strain of OriginMixed stock
Gene Symbol and Name Myo15, myosin XV
Chromosome 11
Gene Common Name(s) DFNB3; RGD1561873; sh-2; sh2; shaker 2;
General Note The Myo15sh2 mutation was discovered by Dobrovolskaia-Zavadskaia (J:15021) in descendants of an irradiated male. Viability and fertility are nearly normal. This mutant is very similar in behavior and pathology to Myo7ash1, with the exception that the abnormalities are observed a little earlier in Myo15sh2. Homozygotes appear to be deaf from the beginning, and the saccular macula is abnormal at birth (J:13046). The stria vascularis appears normal by light microscopy at 2 weeks of age, but begins to show degenerative changes shortly thereafter. Many of the cells contain electron-dense inclusions filled with a fine granular material (J:6781). At 2.5 months old, the type I hair cells of the cristae ampullares and maculae utriculi contain rod-shaped inclusion bodies composed of actin filaments (J:6873).Myo15sh2 was shown by complementation tests to be at a different locus from Myo7ash1 (J:28674), and linkage with Egfr on Chr 11 was established (J:303). Detailed linkage and physical maps of the Myo15sh2 region of Chr 11 have been constructed (J:40351).
Molecular Note The sequences of all 49 exons of this gene were analyzed. A G to A transition was found in codon 674 within exon 18 that produces a Cys to Tyr substitution that likely removes an actin binding site. [MGI Ref ID J:48001]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Anderson DW; Probst FJ; Belyantseva IA; Fridell RA; Beyer L; Martin DM; Wu D; Kachar B; Friedman TB; Raphael Y; Camper SA. 2000. The motor and tail regions of myosin XV are critical for normal structure and function of auditory and vestibular hair cells Hum Mol Genet 9(12):1729-38. [PubMed: 10915760]  [MGI Ref ID J:63740]

Anniko M. 1982. Specific pathology of the stria vascularis in postnatal progressive genetic inner ear disorder. Hear Res 6(3):247-58. [PubMed: 6979536]  [MGI Ref ID J:6781]

Belyantseva IA; Boger ET; Friedman TB. 2003. Myosin XVa localizes to the tips of inner ear sensory cell stereocilia and is essential for staircase formation of the hair bundle. Proc Natl Acad Sci U S A 100(24):13958-63. [PubMed: 14610277]  [MGI Ref ID J:92621]

Erichsen S; Mikkola M; Sahlin L; Hultcrantz M. 2001. Cochlear distribution of Na,K-ATPase and corticosteroid receptors in two mouse strains with congenital hearing disorders. Acta Otolaryngol 121(7):794-802. [PubMed: 11718241]  [MGI Ref ID J:92589]

Lee DJ; Cahill HB; Ryugo DK. 2003. Effects of congenital deafness in the cochlear nuclei of Shaker-2 mice: an ultrastructural analysis of synapse morphology in the endbulbs of Held. J Neurocytol 32(3):229-43. [PubMed: 14724386]  [MGI Ref ID J:89801]

Probst FJ; Fridell RA; Raphael Y; Saunders TL; Wang A; Liang Y ; Morell RJ ; Touchman JW ; Lyons RH ; Noben-Trauth K ; Friedman TB ; Camper SA. 1998. Correction of deafness in shaker-2 mice by an unconventional myosin in a BAC transgene [see comments] Science 280(5368):1444-7. [PubMed: 9603735]  [MGI Ref ID J:48001]

Myo15sh2 related

Anderson DW; Probst FJ; Belyantseva IA; Fridell RA; Beyer L; Martin DM; Wu D; Kachar B; Friedman TB; Raphael Y; Camper SA. 2000. The motor and tail regions of myosin XV are critical for normal structure and function of auditory and vestibular hair cells Hum Mol Genet 9(12):1729-38. [PubMed: 10915760]  [MGI Ref ID J:63740]

Anniko M. 1982. Specific pathology of the stria vascularis in postnatal progressive genetic inner ear disorder. Hear Res 6(3):247-58. [PubMed: 6979536]  [MGI Ref ID J:6781]

Belyantseva IA; Boger ET; Friedman TB. 2003. Myosin XVa localizes to the tips of inner ear sensory cell stereocilia and is essential for staircase formation of the hair bundle. Proc Natl Acad Sci U S A 100(24):13958-63. [PubMed: 14610277]  [MGI Ref ID J:92621]

Belyantseva IA; Boger ET; Naz S; Frolenkov GI; Sellers JR; Ahmed ZM; Griffith AJ; Friedman TB. 2005. Myosin-XVa is required for tip localization of whirlin and differential elongation of hair-cell stereocilia. Nat Cell Biol 7(2):148-56. [PubMed: 15654330]  [MGI Ref ID J:102099]

Beyer LA; Odeh H; Probst FJ; Lambert EH; Dolan DF; Camper SA; Kohrman DC; Raphael Y. 2000. Hair cells in the inner ear of the pirouette and shaker 2 mutant mice. J Neurocytol 29(4):227-40. [PubMed: 11276175]  [MGI Ref ID J:94385]

Bossi G; Booth S; Clark R; Davis EG; Liesner R; Richards K; Starcevic M; Stinchcombe J; Trambas C; Dell'Angelica EC; Griffiths GM. 2005. Normal lytic granule secretion by cytotoxic T lymphocytes deficient in BLOC-1, -2 and -3 and myosins Va, VIIa and XV. Traffic 6(3):243-51. [PubMed: 15702992]  [MGI Ref ID J:105404]

CARTER TC; PHILLIPS RJ. 1953. The sex distribution of waved-2, shaker-2 and Rex in the house mouse. Z Indukt Abstamm Vererbungsl 85(4):564-78. [PubMed: 13170359]  [MGI Ref ID J:225]

Calderon A; Derr A; Stagner BB; Johnson KR; Martin G; Noben-Trauth K. 2006. Cochlear developmental defect and background-dependent hearing thresholds in the Jackson circler (jc) mutant mouse. Hear Res 221(1-2):44-58. [PubMed: 16962269]  [MGI Ref ID J:113021]

Clark FH. 1935. Linkage relations of Zavadskaia Shaker in the house mouse (Mus musculus) Proc Natl Acad Sci U S A 21(5):247-51. [PubMed: 16577664]  [MGI Ref ID J:28674]

Deol MS. 1954. The anomalies of the labyrinth of the mutants varitint-waddler, shaker-2 and jerker in the mouse. J Genet 52:562-588.  [MGI Ref ID J:13046]

Dobrovolskaia-Zavasdkaia N. 1928. L'irradiation des testicules et l'heredite chez la souris Arch Biol (Liege) 38:457-501.  [MGI Ref ID J:15021]

Erichsen S; Mikkola M; Sahlin L; Hultcrantz M. 2001. Cochlear distribution of Na,K-ATPase and corticosteroid receptors in two mouse strains with congenital hearing disorders. Acta Otolaryngol 121(7):794-802. [PubMed: 11718241]  [MGI Ref ID J:92589]

Etournay R; Lepelletier L; Boutet de Monvel J; Michel V; Cayet N; Leibovici M; Weil D; Foucher I; Hardelin JP; Petit C. 2010. Cochlear outer hair cells undergo an apical circumference remodeling constrained by the hair bundle shape. Development 137(8):1373-83. [PubMed: 20332152]  [MGI Ref ID J:158897]

KOCHER W. 1960. [Studies on the genetics and pathology of the development of 8 labyrinth mutants (deaf-waltzer-shaker mutants) in the mouse (Mus musculus).] Z Vererbungsl 91:114-40. [PubMed: 13853422]  [MGI Ref ID J:15164]

Kanzaki S; Beyer L; Karolyi IJ; Dolan DF; Fang Q; Probst FJ; Camper SA; Raphael Y. 2006. Transgene correction maintains normal cochlear structure and function in 6-month-old Myo15a mutant mice. Hear Res 214(1-2):37-44. [PubMed: 16580798]  [MGI Ref ID J:108202]

Karolyi IJ; Probst FJ; Beyer L; Odeh H; Dootz G; Cha KB; Martin DM; Avraham KB; Kohrman D; Dolan DF; Raphael Y; Camper SA. 2003. Myo15 function is distinct from Myo6, Myo7a and pirouette genes in development of cochlear stereocilia. Hum Mol Genet 12(21):2797-805. [PubMed: 12966030]  [MGI Ref ID J:86379]

Kikkawa Y; Mburu P; Morse S; Kominami R; Townsend S; Brown SD. 2005. Mutant analysis reveals whirlin as a dynamic organizer in the growing hair cell stereocilium. Hum Mol Genet 14(3):391-400. [PubMed: 15590699]  [MGI Ref ID J:95916]

Lee DJ; Cahill HB; Ryugo DK. 2003. Effects of congenital deafness in the cochlear nuclei of Shaker-2 mice: an ultrastructural analysis of synapse morphology in the endbulbs of Held. J Neurocytol 32(3):229-43. [PubMed: 14724386]  [MGI Ref ID J:89801]

Mburu P; Kikkawa Y; Townsend S; Romero R; Yonekawa H; Brown SD. 2006. Whirlin complexes with p55 at the stereocilia tip during hair cell development. Proc Natl Acad Sci U S A 103(29):10973-8. [PubMed: 16829577]  [MGI Ref ID J:111803]

Probst FJ; Fridell RA; Raphael Y; Saunders TL; Wang A; Liang Y ; Morell RJ ; Touchman JW ; Lyons RH ; Noben-Trauth K ; Friedman TB ; Camper SA. 1998. Correction of deafness in shaker-2 mice by an unconventional myosin in a BAC transgene [see comments] Science 280(5368):1444-7. [PubMed: 9603735]  [MGI Ref ID J:48001]

Prosser HM; Rzadzinska AK; Steel KP; Bradley A. 2008. Mosaic complementation demonstrates a regulatory role for myosin VIIa in actin dynamics of stereocilia. Mol Cell Biol 28(5):1702-12. [PubMed: 18160714]  [MGI Ref ID J:132633]

Snell GD; Law LW. 1939. A linkage between shaker-2 and wavy-2 in the house mouse J Hered 30:447.  [MGI Ref ID J:303]

Sobin A; Anniko M; Flock A. 1982. Rods of actin filaments in type I hair cells of the Shaker-2 mouse. Arch Otorhinolaryngol 236(1):1-6. [PubMed: 6889851]  [MGI Ref ID J:6873]

Stepanyan R; Frolenkov GI. 2009. Fast adaptation and Ca2+ sensitivity of the mechanotransducer require myosin-XVa in inner but not outer cochlear hair cells. J Neurosci 29(13):4023-34. [PubMed: 19339598]  [MGI Ref ID J:147447]

Wakabayashi Y; Kikkawa Y; Matsumoto Y; Shinbo T; Kosugi S; Chou D; Furuya M; Jishage K; Noda T; Yonekawa H; Kominami R. 1997. Genetic and physical delineation of the region of the mouse deafness mutation shaker-2. Biochem Biophys Res Commun 234(1):107-10. [PubMed: 9168970]  [MGI Ref ID J:40351]

Wakabayashi Y; Takahashi Y; Kikkawa Y; Okano H; Mishima Y; Ushiki T ; Yonekawa H ; Kominami R. 1998. A novel type of myosin encoded by the mouse deafness gene shaker-2. Biochem Biophys Res Commun 248(3):655-9. [PubMed: 9703981]  [MGI Ref ID J:49063]

Webster DB; Sobin A; Anniko M. 1986. Incomplete maturation of brainstem auditory nuclei in genetically induced early postnatal cochlear degeneration. Acta Otolaryngol 101(5-6):429-38. [PubMed: 3727977]  [MGI Ref ID J:94384]

Wright ME. 1947. Two sex-linkages in the house mouse, with unusual recombination values Heredity 1:349-54.  [MGI Ref ID J:313]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryMyo15sh2/Myo15sh2 males may trample pups due to circling behavior; remove the male from the cage when the female is pregnant.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

General Supply Notes

  • View the complete collection of spontaneous mutants in the Mouse Mutant Resource.

Control Information

  Control
   Heterozygote from the colony
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.8)