Strain Name:

B6.C3-KitW-44J/J

Stock Number:

000122

Availability:

Repository-Cryopreserved

Description

Strain Information

Former Names C57BL/6J-KitW-44J    (Changed: 15-DEC-04 )
Type Coisogenic; Mutant Strain; Spontaneous Mutation;
Additional information on Genetically Engineered Mutant Mice.
Specieslaboratory mouse
Background Strain C57BL/6J
GenerationN34

Description
KitW-44J heterozygotes have white-tipped feet and a white tail tip although the belly spot standardly found in KitW* mutations is very small in this mutant, sometimes restricted to only a few hairs. Homozygotes have a flecked pelt that is predominantly white, especially ventrally, with pigmented patches particularly at the lateral borders. The pigmentation fades with age yielding black-eyed white mice by approximately 9 months of age. While many KitW-44J homozygotes are viable, fertility is diminished. Homozygous females have greatly reduced fertility and gonads that are smaller in size with reduced activity. Homozygous males are sterile although spermatogenesis occurs. The KitW-44J allele does not produce anemia in either its heterozygous or homozygous state. The red blood cell count, white blood cell count, hematocrit, and mean cell volume are normal. However, bone marrow transplantation experiments reveal that the colony forming units in the bone marrow of KitW-44J homozygotes are much lower than normal despite the fact that the bone marrow has a normal cell count. While bone marrow transplantation from KitW-44J homozygotes can cure anemia in KitW/KitW-v hosts, it repopulates more slowly than does wild type bone marrow. Bone marrow from KitW-44J homozygotes also fails to repopulate the mast cells in the skin of KitW/KitW-v hosts. Wild type bone marrow transplanted into non-irradiated hosts out-competes the endogenous erythropoeisis in KitW-44J homozygotes but not in wild type hosts, further confirming a deficiency in erythropoeisis in these non-anemic mice. Mice doubly heterozygous for KitW-44J and KitW-39J are more mildly anemic than mice doubly heterozygous for KitW-39J and other second KitW* alleles. (Geissler et al., 1981; Geissler and Russell, 1983; Sawada et al., 1991.)

Development
The KitW-44J mutation arose spontaneously at the Jackson Laboratory on the C3H/HeJ background in the mid-1970s. It was backcrossed to C57BL/6J and in 1980 heterozygous males at N33 were bred with C57BL/6J females to produce embryos for cryopreservation. (Geissler et al., 1981 and 1988.)

Related Strains

View Strains carrying other alleles of Kit     (36 strains)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

KitW-44J/Kit+

        B6.C3-KitW-44J
  • pigmentation phenotype
  • white spotting (MGI Ref ID J:6571)
    • white feet and tail tip
    • belly spot (MGI Ref ID J:6571)
      • very small spot to only a few hairs
  • hematopoietic system phenotype
  • *normal* hematopoietic system phenotype (MGI Ref ID J:6571)
    • no hematopoietic system abnormalities detected
  • skin/coat/nails phenotype
  • white spotting (MGI Ref ID J:6571)
    • white feet and tail tip
    • belly spot (MGI Ref ID J:6571)
      • very small spot to only a few hairs

KitW-44J/KitW-44J

        B6.C3-KitW-44J
  • reproductive system phenotype
  • abnormal ovary morphology (MGI Ref ID J:6571)
    • pathological invagination of the germinal epithelium by 2 months of age
    • abnormal ovarian follicle number (MGI Ref ID J:6571)
      • ovaries contain fewer developing follicles than in wild-type mice
    • small ovary (MGI Ref ID J:6571)
  • decreased litter size (MGI Ref ID J:6571)
    • some female mice produce small litters
  • male infertility (MGI Ref ID J:6571)
    • testes are less than a third normal size and devoid of spermatogenic activity
  • reduced female fertility (MGI Ref ID J:6571)
    • 50% are fertile and these produce one or two small litters
  • skin/coat/nails phenotype
  • irregular coat pigmentation (MGI Ref ID J:6571)
    • mice exhibit a black-flecked white coat in which the dorsal coat has concentrated areas of black about the head and hind quarters and the ventral coat is white with black hair along lateral borders
  • hematopoietic system phenotype
  • *normal* hematopoietic system phenotype (MGI Ref ID J:6571)
    • no hematopoietic system abnormalities detected
  • pigmentation phenotype
  • irregular coat pigmentation (MGI Ref ID J:6571)
    • mice exhibit a black-flecked white coat in which the dorsal coat has concentrated areas of black about the head and hind quarters and the ventral coat is white with black hair along lateral borders
  • endocrine/exocrine gland phenotype
  • abnormal ovary morphology (MGI Ref ID J:6571)
    • pathological invagination of the germinal epithelium by 2 months of age
    • abnormal ovarian follicle number (MGI Ref ID J:6571)
      • ovaries contain fewer developing follicles than in wild-type mice
    • small ovary (MGI Ref ID J:6571)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

KitW-44J related

Cancer Research
Growth Factors/Receptors/Cytokines
Increased Tumor Incidence (Gonadal Tumors: ovarian)
Oncogenes

Dermatology Research
Color and White Spotting Defects

Developmental Biology Research
Neural Crest Defects

Endocrine Deficiency Research
Bone/Bone Marrow Defects
Gonad Defects
Skin Defects

Immunology and Inflammation Research
Immunodeficiency (Mast Cell Deficiency)

Mouse/Human Gene Homologs
piebaldism

Neurobiology Research
Receptor Defects
Vestibular and Hearing Defects

Reproductive Biology Research
Developmental Defects Affecting Gonads (germ cell deficient)
Fertility Defects
Gonadal Tumors (ovarian)

Research Tools
Immunology and Inflammation Research (Mast Cell Deficiency)

Sensorineural Research
Vestibular and Hearing Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol KitW-44J
Allele Name dominant spotting 44 Jackson
Allele Type Spontaneous
Strain of OriginC3H/HeJ
Gene Symbol and Name Kit, kit oncogene
Chromosome 5
Gene Common Name(s) Bs; C-Kit; CD117; Dominant white spotting; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; PBT; SCFR; SCO1; SCO5; SOW3; Ssm; Steel Factor Receptor; Tr-kit; W; belly-spot; dominant spotting; gsf spotted coat 1; gsf spotted coat 5; phenotype like Sl or W 3; spotted sterile male;
Molecular Note Southern hybridization analyses of genomic DNA using probes corresponding to two specific lengths of amino acids from c-kit suggests that this allele comprises a 4-5 kb insertion that disrupts c-kit. The level of c-kit mRNA in homozygotes is markedly reduced. [MGI Ref ID J:9374]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Additional References

Arguello F; Furlanetto RW; Baggs RB; Graves BT; Harwell SE; Cohen HJ; Frantz CN. 1992. Incidence and distribution of experimental metastases in mutant mice with defective organ microenvironments (genotypes Sl/Sld and W/Wv). Cancer Res 52(8):2304-9. [PubMed: 1559233]  [MGI Ref ID J:468]

Geissler EN; McFarland EC; Russell ES. 1981. Analysis of pleiotropism at the dominant white-spotting (W) locus of the house mouse: a description of ten new W alleles. Genetics 97(2):337-61. [PubMed: 7274658]  [MGI Ref ID J:6571]

Geissler EN; Russell ES. 1983. Analysis of the hematopoietic effects of new dominant spotting (W) mutations of the mouse. I. Influence upon hematopoietic stem cells. Exp Hematol 11(6):452-60. [PubMed: 6352297]  [MGI Ref ID J:7186]

Geissler EN; Russell ES. 1983. Analysis of the hematopoietic effects of new dominant spotting (W) mutations of the mouse. II. Effects on mast cell development. Exp Hematol 11(6):461-6. [PubMed: 6352298]  [MGI Ref ID J:7187]

Geissler EN; Ryan MA; Housman DE. 1988. The dominant-white spotting (W) locus of the mouse encodes the c-kit proto-oncogene. Cell 55(1):185-92. [PubMed: 2458842]  [MGI Ref ID J:9374]

Kitamura Y; Kasugai T; Ebi Y; Nomura S. 1991. Fibroblast-dependent differentiation/proliferation of mast cells. Skin Pharmacol 4 Suppl 1:2-7. [PubMed: 1764248]  [MGI Ref ID J:619]

Murphy ED. 1972. Hyperplastic and early neoplastic changes in the ovaries of mice after genic deletion of germ cells. J Natl Cancer Inst 48(5):1283-95. [PubMed: 4337905]  [MGI Ref ID J:5274]

Nocka K; Tan JC; Chiu E; Chu TY; Ray P; Traktman P; Besmer P. 1990. Molecular bases of dominant negative and loss of function mutations at the murine c-kit/white spotting locus: W37, Wv, W41 and W. EMBO J 9(6):1805-13. [PubMed: 1693331]  [MGI Ref ID J:10528]

Sawada K; Sakamaki K; Nishimune Y. 1991. Effect of the W mutation, for white belly spot, on testicular germ cell differentiation in mice. J Reprod Fertil 93(2):287-94. [PubMed: 1686280]  [MGI Ref ID J:1856]

KitW-44J related

Geissler EN; McFarland EC; Russell ES. 1981. Analysis of pleiotropism at the dominant white-spotting (W) locus of the house mouse: a description of ten new W alleles. Genetics 97(2):337-61. [PubMed: 7274658]  [MGI Ref ID J:6571]

Geissler EN; Ryan MA; Housman DE. 1988. The dominant-white spotting (W) locus of the mouse encodes the c-kit proto-oncogene. Cell 55(1):185-92. [PubMed: 2458842]  [MGI Ref ID J:9374]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $1900.00
*Price(s) in US dollars ($)

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $2470.00
*Price(s) in US dollars ($)

Additional Supply Details

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • Genomic DNA is available for this strain from the Mouse DNA Resource.

General Terms and Conditions


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