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Strain Name:

B6.C3-KitW-44J/J

Stock Number:

000122

Availability:

Repository-Cryopreserved

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General Terms and Conditions

Former Name      C57BL/6J-KitW-44J    (Changed: 15-DEC-04 )
Genes & Alleles   Kit;   KitW-44J;

Product Information

Strain Details

Type JAX® GEMM® Strain - Coisogenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Spontaneous Mutation
Specieslaboratory mouse
Background Strain C57BL/6J
GenerationN34

Strain Description
KitW-44J heterozygotes have white-tipped feet and a white tail tip although the belly spot standardly found in KitW* mutations is very small in this mutant, sometimes restricted to only a few hairs. Homozygotes have a flecked pelt that is predominantly white, especially ventrally, with pigmented patches particularly at the lateral borders. The pigmentation fades with age yielding black-eyed white mice by approximately 9 months of age. While many KitW-44J homozygotes are viable, fertility is diminished. Homozygous females have greatly reduced fertility and gonads that are smaller in size with reduced activity. Homozygous males are sterile although spermatogenesis occurs. The KitW-44J allele does not produce anemia in either its heterozygous or homozygous state. The red blood cell count, white blood cell count, hematocrit, and mean cell volume are normal. However, bone marrow transplantation experiments reveal that the colony forming units in the bone marrow of KitW-44J homozygotes are much lower than normal despite the fact that the bone marrow has a normal cell count. While bone marrow transplantation from KitW-44J homozygotes can cure anemia in KitW/KitW-v hosts, it repopulates more slowly than does wild type bone marrow. Bone marrow from KitW-44J homozygotes also fails to repopulate the mast cells in the skin of KitW/KitW-v hosts. Wild type bone marrow transplanted into non-irradiated hosts out-competes the endogenous erythropoeisis in KitW-44J homozygotes but not in wild type hosts, further confirming a deficiency in erythropoeisis in these non-anemic mice. Mice doubly heterozygous for KitW-44J and KitW-39J are more mildly anemic than mice doubly heterozygous for KitW-39J and other second KitW* alleles. (Geissler et al., 1981; Geissler and Russell, 1983; Sawada et al., 1991.)

Strain Development
The KitW-44J mutation arose spontaneously at the Jackson Laboratory on the C3H/HeJ background in the mid-1970s. It was backcrossed to C57BL/6J and in 1980 heterozygous males at N33 were bred with C57BL/6J females to produce embryos for cryopreservation. (Geissler et al., 1981 and 1988.)

Mammalian Phenotype Terms assigned by genotype

KitW-44J/Kit+

        C57BL/6J
  • pigmentation phenotype
  • white spotting (MGI Ref ID J:6571)
    • white feet and tail tip
    • belly spot (MGI Ref ID J:6571)
      • very small spot to only a few hairs
  • hematopoietic system phenotype
  • *normal* hematopoietic system phenotype (MGI Ref ID J:6571)
    • no hematopoietic system abnormalities detected
  • skin/coat/nails phenotype
  • white spotting (MGI Ref ID J:6571)
    • white feet and tail tip
    • belly spot (MGI Ref ID J:6571)
      • very small spot to only a few hairs

KitW-44J/KitW-44J

        C57BL/6J
  • pigmentation phenotype
  • irregular coat pigmentation (MGI Ref ID J:6571)
    • black-flecked white coat
    • dorsal coat has concentrated areas of black about the head and hind quarters
    • ventral coat is white with black hair along lateral borders
  • endocrine/exocrine gland phenotype
  • abnormal ovary morphology (MGI Ref ID J:6571)
    • pathological invagination of the germinal epithelium by 2 months of age
  • hematopoietic system phenotype
  • *normal* hematopoietic system phenotype (MGI Ref ID J:6571)
    • no hematopoietic system abnormalities detected
  • reproductive system phenotype
  • abnormal ovary morphology (MGI Ref ID J:6571)
    • pathological invagination of the germinal epithelium by 2 months of age
  • male infertility (MGI Ref ID J:6571)
    • testes are less than a third normal size and devoid of spermatogenic activity
  • reduced female fertility (MGI Ref ID J:6571)
    • 50% are fertile and these produce one or two small litters
  • skin/coat/nails phenotype
  • irregular coat pigmentation (MGI Ref ID J:6571)
    • black-flecked white coat
    • dorsal coat has concentrated areas of black about the head and hind quarters
    • ventral coat is white with black hair along lateral borders

Gene & Allele Details

Allele Symbol KitW-44J
Allele Name dominant spotting 44 Jackson
Strain of OriginC3H/HeJ
Gene Symbol and Name Kit, kit oncogene
Chromosome 5
Gene Common Name(s) Bs; C-Kit; CD117; Dominant white spotting; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; PBT; SCFR; SCO1; SCO5; SOW3; Ssm; Steel Factor Receptor; Tr-kit; W; belly-spot; dominant spotting; gsf spotted coat 1; gsf spotted coat 5; phenotype like Sl or W 3; spotted sterile male;
Molecular Note Southern hybridization analyses of genomic DNA using probes corresponding to two specific lengths of amino acids from c-kit suggests that this allele comprises a 4-5 kb insertion that disrupts c-kit. The level of c-kit mRNA in homozygotes is markedly reduced. [MGI Ref ID J:9374]

Related Strains

View Strains carrying other alleles of Kit     (36 strains)

Additional Web Information

Congenic Nomenclature

Research Applications

This mouse can be used to support research in many areas including:

KitW-44J related

Cancer Research
Growth Factors/Receptors/Cytokines
Increased Tumor Incidence (Gonadal Tumors: ovarian)
Oncogenes

Dermatology Research
Color and White Spotting Defects

Developmental Biology Research
Neural Crest Defects

Endocrine Deficiency Research
Bone/Bone Marrow Defects
Gonad Defects
Skin Defects

Immunology and Inflammation Research
Immunodeficiency (Mast Cell Deficiency)

Mouse/Human Gene Homologs
piebaldism

Neurobiology Research
Receptor Defects
Vestibular and Hearing Defects

Reproductive Biology Research
Developmental Defects Affecting Gonads (germ cell deficient)
Fertility Defects
Gonadal Tumors (ovarian)

Research Tools
Immunology and Inflammation Research (Mast Cell Deficiency)

Sensorineural Research
Vestibular and Hearing Defects

References

Additional References

Price and Supply Information

Strain Name: B6.C3-KitW-44J/J
Stock Number: 000122

Price Details

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
Genomic DNA is available for this strain from the Mouse DNA Resource.

LicensingSee General Terms and Conditions below  

General Terms and Conditions

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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