Strain Name: |
B6.C3-KitW-44J/J |
|---|---|
Stock Number: |
000122 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
C57BL/6J-KitW-44J (Changed: 15-DEC-04
) |
| Genes & Alleles | Kit; KitW-44J; |
Type JAX® GEMM® Strain - Coisogenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Spontaneous Mutation Species laboratory mouse Background Strain C57BL/6J Generation N34 Strain Description
KitW-44J heterozygotes have white-tipped feet and a white tail tip although the belly spot standardly found in KitW* mutations is very small in this mutant, sometimes restricted to only a few hairs. Homozygotes have a flecked pelt that is predominantly white, especially ventrally, with pigmented patches particularly at the lateral borders. The pigmentation fades with age yielding black-eyed white mice by approximately 9 months of age. While many KitW-44J homozygotes are viable, fertility is diminished. Homozygous females have greatly reduced fertility and gonads that are smaller in size with reduced activity. Homozygous males are sterile although spermatogenesis occurs. The KitW-44J allele does not produce anemia in either its heterozygous or homozygous state. The red blood cell count, white blood cell count, hematocrit, and mean cell volume are normal. However, bone marrow transplantation experiments reveal that the colony forming units in the bone marrow of KitW-44J homozygotes are much lower than normal despite the fact that the bone marrow has a normal cell count. While bone marrow transplantation from KitW-44J homozygotes can cure anemia in KitW/KitW-v hosts, it repopulates more slowly than does wild type bone marrow. Bone marrow from KitW-44J homozygotes also fails to repopulate the mast cells in the skin of KitW/KitW-v hosts. Wild type bone marrow transplanted into non-irradiated hosts out-competes the endogenous erythropoeisis in KitW-44J homozygotes but not in wild type hosts, further confirming a deficiency in erythropoeisis in these non-anemic mice. Mice doubly heterozygous for KitW-44J and KitW-39J are more mildly anemic than mice doubly heterozygous for KitW-39J and other second KitW* alleles. (Geissler et al., 1981; Geissler and Russell, 1983; Sawada et al., 1991.)Strain Development
The KitW-44J mutation arose spontaneously at the Jackson Laboratory on the C3H/HeJ background in the mid-1970s. It was backcrossed to C57BL/6J and in 1980 heterozygous males at N33 were bred with C57BL/6J females to produce embryos for cryopreservation. (Geissler et al., 1981 and 1988.)
Mammalian Phenotype Terms assigned by genotype |
| Allele Symbol | KitW-44J | ||
|---|---|---|---|
| Allele Name | dominant spotting 44 Jackson | ||
| Strain of Origin | C3H/HeJ | ||
| Gene Symbol and Name | Kit, kit oncogene | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | Bs; C-Kit; CD117; Dominant white spotting; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; PBT; SCFR; SCO1; SCO5; SOW3; Ssm; Steel Factor Receptor; Tr-kit; W; belly-spot; dominant spotting; gsf spotted coat 1; gsf spotted coat 5; phenotype like Sl or W 3; spotted sterile male; | ||
| Molecular Note | Southern hybridization analyses of genomic DNA using probes corresponding to two specific lengths of amino acids from c-kit suggests that this allele comprises a 4-5 kb insertion that disrupts c-kit. The level of c-kit mRNA in homozygotes is markedly reduced. [MGI Ref ID J:9374] | ||
Strains carrying other alleles of Kit
000599 B6 x B6CBCa Aw-J/A-T(5;13)264Ca KitW-v/J 006564 B6(C)-KitW-41J Gusbmps/BrkJ 000495 B6.C-H38c/By-KitW-56J/J 000560 B6.C-H7b/By KitW-50J/J 000991 B6.C58-KitW-57J/J 002283 B6.Cg-KitW-19H/EiJ 000133 B6.Cg-KitW-24J/J 000139 B6.Cg-KitW-25J/J 005051 B6.Cg-KitW-sh/HNihrJaeBsmJ 000164 B6.Cg-KitW/J 000194 B6.Cg-Lx KitW-v/J 000171 B6.D2-KitW-45J/J 001563 B6.D2-KitW-73J/J 001177 B6.LP-KitW-49J/J 000350 B6By.Cg-KitW-v MitfMi-wh T/J 000627 C3H/HeJ-KitW-x/J 000847 C3Sn.B6-KitW-39J/J 000166 C57BL/6J-KitW-17J/J 000167 C57BL/6J-KitW-18J/J 000169 C57BL/6J-KitW-20J/J 000117 C57BL/6J-KitW-34J/J 000128 C57BL/6J-KitW-35J/J 000134 C57BL/6J-KitW-37J/J 000062 C57BL/6J-KitW-39J/J 000121 C57BL/6J-KitW-40J/J 000119 C57BL/6J-KitW-41J/J 000127 C57BL/6J-KitW-42J/J 000129 C57BL/6J-KitW-43J/J 000990 C57BL/6J-KitW-55J/J 001179 C57BL/6J-KitW-62J/J 000049 C57BL/6J-KitW-v/J 000965 CBACa.C3-KitW-x/J 000092 FL/1Re-KitW/J 000993 NZB/BlNJ-KitW-59J/J 000692 WB/ReJ KitW/J 100410 WBB6F1/J-KitW/KitW-v/J View Strains carrying other alleles of Kit (36 strains)
Congenic Nomenclature
KitW-44J related
Cancer Research
Growth Factors/Receptors/Cytokines
Increased Tumor Incidence (Gonadal Tumors: ovarian)
Oncogenes
Dermatology Research
Color and White Spotting Defects
Developmental Biology Research
Neural Crest Defects
Endocrine Deficiency Research
Bone/Bone Marrow Defects
Gonad Defects
Skin Defects
Immunology and Inflammation Research
Immunodeficiency (Mast Cell Deficiency)
Mouse/Human Gene Homologs
piebaldism
Neurobiology Research
Receptor Defects
Vestibular and Hearing Defects
Reproductive Biology Research
Developmental Defects Affecting Gonads (germ cell deficient)
Fertility Defects
Gonadal Tumors (ovarian)
Research Tools
Immunology and Inflammation Research (Mast Cell Deficiency)
Sensorineural Research
Vestibular and Hearing Defects
| Strain Name: | B6.C3-KitW-44J/J |
| Stock Number: | 000122 |
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
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