Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names C57BL/6J-KitW-44J    (Changed: 15-DEC-04 ) Type Coisogenic; Mutant Strain; Spontaneous Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Background Strain C57BL/6J Generation N34 Generation Definitions

Description
Kit^W-44J heterozygotes have white-tipped feet and a white tail tip although the belly spot standardly found in Kit^W* mutations is very small in this mutant, sometimes restricted to only a few hairs. Homozygotes have a flecked pelt that is predominantly white, especially ventrally, with pigmented patches particularly at the lateral borders. The pigmentation fades with age yielding black-eyed white mice by approximately 9 months of age. While many Kit^W-44J homozygotes are viable, fertility is diminished. Homozygous females have greatly reduced fertility and gonads that are smaller in size with reduced activity. Homozygous males are sterile although spermatogenesis occurs. The Kit^W-44J allele does not produce anemia in either its heterozygous or homozygous state. The red blood cell count, white blood cell count, hematocrit, and mean cell volume are normal. However, bone marrow transplantation experiments reveal that the colony forming units in the bone marrow of Kit^W-44J homozygotes are much lower than normal despite the fact that the bone marrow has a normal cell count. While bone marrow transplantation from Kit^W-44J homozygotes can cure anemia in Kit^W/Kit^W-v hosts, it repopulates more slowly than does wild type bone marrow. Bone marrow from Kit^W-44J homozygotes also fails to repopulate the mast cells in the skin of Kit^W/Kit^W-v hosts. Wild type bone marrow transplanted into non-irradiated hosts out-competes the endogenous erythropoeisis in Kit^W-44J homozygotes but not in wild type hosts, further confirming a deficiency in erythropoeisis in these non-anemic mice. Mice doubly heterozygous for Kit^W-44J and Kit^W-39J are more mildly anemic than mice doubly heterozygous for Kit^W-39J and other second Kit^W* alleles. (Geissler et al., 1981; Geissler and Russell, 1983; Sawada et al., 1991.)

Development
The Kit^W-44J mutation arose spontaneously at the Jackson Laboratory on the C3H/HeJ background in the mid-1970s. It was backcrossed to C57BL/6J and in 1980 heterozygous males at N33 were bred with C57BL/6J females to produce embryos for cryopreservation. (Geissler et al., 1981 and 1988.)

Related Strains

Strains carrying other alleles of Kit

000599	B6 x B6CBCa Aw-J/A-T(5;13)264Ca KitW-v/J
006564	B6(C)-KitW-41J Gusbmps/BrkJ
015813	B6.129S7-Kittm1Rosay/J
000495	B6.C-H38c/By-KitW-56J/J
000560	B6.C-H7b/By KitW-50J/J
000991	B6.C58-KitW-57J/J
002283	B6.Cg-KitW-19H/EiJ
000133	B6.Cg-KitW-24J/J
000139	B6.Cg-KitW-25J/J
012861	B6.Cg-KitW-sh/HNihrJaeBsmGlliJ
000164	B6.Cg-KitW/J
000194	B6.Cg-Lx KitW-v/J
000171	B6.D2-KitW-45J/J
001563	B6.D2-KitW-73J/J
001177	B6.LP-KitW-49J/J
000350	B6By.Cg-KitW-v MitfMi-wh T/J
000627	C3H/HeJ-KitW-x/J
000847	C3Sn.B6-KitW-39J/J
000166	C57BL/6J-KitW-17J/J
000167	C57BL/6J-KitW-18J/J
000169	C57BL/6J-KitW-20J/J
000117	C57BL/6J-KitW-34J/J
000128	C57BL/6J-KitW-35J/J
000134	C57BL/6J-KitW-37J/J
000062	C57BL/6J-KitW-39J/J
000121	C57BL/6J-KitW-40J/J
000119	C57BL/6J-KitW-41J/J
000127	C57BL/6J-KitW-42J/J
000129	C57BL/6J-KitW-43J/J
000990	C57BL/6J-KitW-55J/J
001179	C57BL/6J-KitW-62J/J
000049	C57BL/6J-KitW-v/J
000965	CBACa.C3-KitW-x/J
000092	FL/1Re-KitW/J
000993	NZB/BlNJ-KitW-59J/J
005051	STOCK KitW-sh/HNihrJaeBsmJ
000692	WB/ReJ KitW/J
100410	WBB6F1/J-KitW/KitW-v/J

View Strains carrying other alleles of Kit     (38 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI

- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Gastrointestinal Stromal Tumor; GIST   (KIT) Leukemia, Acute Myeloid; AML   (KIT) Mast Cell Disease   (KIT) Piebald Trait; PBT   (KIT) Testicular Germ Cell Tumor; TGCT   (KIT)

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Kit^W-44J/Kit⁺

        B6.C3-Kit^W-44J

• pigmentation phenotype
• white spotting
  • white feet and tail tip   (MGI Ref ID J:6571)
• • belly spot
    • very small spot to only a few hairs   (MGI Ref ID J:6571)
• hematopoietic system phenotype
• *normal* hematopoietic system phenotype
  • no hematopoietic system abnormalities detected   (MGI Ref ID J:6571)
• integument phenotype
• white spotting
  • white feet and tail tip   (MGI Ref ID J:6571)
• • belly spot
    • very small spot to only a few hairs   (MGI Ref ID J:6571)

Kit^W-44J/Kit^W-44J

        B6.C3-Kit^W-44J

• reproductive system phenotype
• abnormal ovary morphology
  • pathological invagination of the germinal epithelium by 2 months of age   (MGI Ref ID J:6571)
• • abnormal ovarian follicle number
    • ovaries contain fewer developing follicles than in wild-type mice   (MGI Ref ID J:6571)
  • small ovary   (MGI Ref ID J:6571)
• decreased litter size
  • some female mice produce small litters   (MGI Ref ID J:6571)
• male infertility
  • testes are less than a third normal size and devoid of spermatogenic activity   (MGI Ref ID J:6571)
• reduced female fertility
  • 50% are fertile and these produce one or two small litters   (MGI Ref ID J:6571)
• hematopoietic system phenotype
• *normal* hematopoietic system phenotype
  • no hematopoietic system abnormalities detected   (MGI Ref ID J:6571)
• pigmentation phenotype
• irregular coat pigmentation
  • mice exhibit a black-flecked white coat in which the dorsal coat has concentrated areas of black about the head and hind quarters and the ventral coat is white with black hair along lateral borders   (MGI Ref ID J:6571)
• endocrine/exocrine gland phenotype
• abnormal ovary morphology
  • pathological invagination of the germinal epithelium by 2 months of age   (MGI Ref ID J:6571)
• • abnormal ovarian follicle number
    • ovaries contain fewer developing follicles than in wild-type mice   (MGI Ref ID J:6571)
  • small ovary   (MGI Ref ID J:6571)
• integument phenotype
• irregular coat pigmentation
  • mice exhibit a black-flecked white coat in which the dorsal coat has concentrated areas of black about the head and hind quarters and the ventral coat is white with black hair along lateral borders   (MGI Ref ID J:6571)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Kit^W-44J related

Cancer Research
Growth Factors/Receptors/Cytokines
Increased Tumor Incidence
      Gonadal Tumors
      Gonadal Tumors: ovarian
Oncogenes

Dermatology Research
Color and White Spotting Defects

Developmental Biology Research
Neural Crest Defects

Endocrine Deficiency Research
Bone/Bone Marrow Defects
Gonad Defects
Skin Defects

Immunology, Inflammation and Autoimmunity Research
Immunodeficiency
      Mast Cell Deficiency

Neurobiology Research
Hearing Defects
Receptor Defects

Reproductive Biology Research
Developmental Defects Affecting Gonads
      germ cell deficient
Fertility Defects
Gonadal Tumors
      ovarian

Research Tools
Immunology and Inflammation Research
      Mast Cell Deficiency

Sensorineural Research
Hearing Defects

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		KitW-44J
Allele Name		dominant spotting 44 Jackson
Allele Type		Spontaneous
Strain of Origin		C3H/HeJ
Gene Symbol and Name		Kit, kit oncogene
Chromosome		5
Gene Common Name(s)		Bs; C-Kit; CD117; Dominant white spotting; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; PBT; SCFR; SCO1; SCO5; SOW3; Ssm; Steel Factor Receptor; Tr-kit; W; belly-spot; dominant spotting; gsf spotted coat 1; gsf spotted coat 5; phenotype like Sl or W 3; spotted sterile male;
Molecular Note		Southern hybridization analyses of genomic DNA using probes corresponding to two specific lengths of amino acids from c-kit suggests that this allele comprises a 4-5 kb insertion that disrupts c-kit. The level of c-kit mRNA in homozygotes is markedly reduced. [MGI Ref ID J:9374]

Genotyping

Genotyping Information

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Arguello F; Furlanetto RW; Baggs RB; Graves BT; Harwell SE; Cohen HJ; Frantz CN. 1992. Incidence and distribution of experimental metastases in mutant mice with defective organ microenvironments (genotypes Sl/Sld and W/Wv). Cancer Res 52(8):2304-9. [PubMed: 1559233]  [MGI Ref ID J:468]

Geissler EN; McFarland EC; Russell ES. 1981. Analysis of pleiotropism at the dominant white-spotting (W) locus of the house mouse: a description of ten new W alleles. Genetics 97(2):337-61. [PubMed: 7274658]  [MGI Ref ID J:6571]

Geissler EN; Russell ES. 1983. Analysis of the hematopoietic effects of new dominant spotting (W) mutations of the mouse. I. Influence upon hematopoietic stem cells. Exp Hematol 11(6):452-60. [PubMed: 6352297]  [MGI Ref ID J:7186]

Geissler EN; Russell ES. 1983. Analysis of the hematopoietic effects of new dominant spotting (W) mutations of the mouse. II. Effects on mast cell development. Exp Hematol 11(6):461-6. [PubMed: 6352298]  [MGI Ref ID J:7187]

Geissler EN; Ryan MA; Housman DE. 1988. The dominant-white spotting (W) locus of the mouse encodes the c-kit proto-oncogene. Cell 55(1):185-92. [PubMed: 2458842]  [MGI Ref ID J:9374]

Kitamura Y; Kasugai T; Ebi Y; Nomura S. 1991. Fibroblast-dependent differentiation/proliferation of mast cells. Skin Pharmacol 4 Suppl 1:2-7. [PubMed: 1764248]  [MGI Ref ID J:619]

Murphy ED. 1972. Hyperplastic and early neoplastic changes in the ovaries of mice after genic deletion of germ cells. J Natl Cancer Inst 48(5):1283-95. [PubMed: 4337905]  [MGI Ref ID J:5274]

Nocka K; Tan JC; Chiu E; Chu TY; Ray P; Traktman P; Besmer P. 1990. Molecular bases of dominant negative and loss of function mutations at the murine c-kit/white spotting locus: W37, Wv, W41 and W. EMBO J 9(6):1805-13. [PubMed: 1693331]  [MGI Ref ID J:10528]

Sawada K; Sakamaki K; Nishimune Y. 1991. Effect of the W mutation, for white belly spot, on testicular germ cell differentiation in mice. J Reprod Fertil 93(2):287-94. [PubMed: 1686280]  [MGI Ref ID J:1856]

Kit^W-44J related

Geissler EN; McFarland EC; Russell ES. 1981. Analysis of pleiotropism at the dominant white-spotting (W) locus of the house mouse: a description of ten new W alleles. Genetics 97(2):337-61. [PubMed: 7274658]  [MGI Ref ID J:6571]

Geissler EN; Ryan MA; Housman DE. 1988. The dominant-white spotting (W) locus of the mouse encodes the c-kit proto-oncogene. Cell 55(1):185-92. [PubMed: 2458842]  [MGI Ref ID J:9374]

Reith AD; Rottapel R; Giddens E; Brady C; Forrester L; Bernstein A. 1990. W mutant mice with mild or severe developmental defects contain distinct point mutations in the kinase domain of the c-kit receptor. Genes Dev 4(3):390-400. [PubMed: 1692559]  [MGI Ref ID J:28221]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

General Supply Notes

• Genomic DNA is available for this strain from the Mouse DNA Resource.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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