Strain Name:

C57BL/6J-KitW-42J/J

Stock Number:

000127

Availability:

Repository-Cryopreserved

Description

Strain Information

Type Coisogenic; Mutant Strain; Spontaneous Mutation;
Additional information on Genetically Engineered Mutant Mice.
Specieslaboratory mouse
GenerationN33p

Appearance
mostly white with black eyes, gray ears, and sometimes a black body spot
Related Genotype: a/a KitW-42/+

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Kit     (36 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

KitW-42J/Kit+

        involves: C57BL/6J
  • reproductive system phenotype
  • abnormal ovarian follicle number (MGI Ref ID J:6571)
  • small testis (MGI Ref ID J:6571)
  • pigmentation phenotype
  • absent coat pigmentation (MGI Ref ID J:6571)
    • white coat with some dark pigmentation on the ears
    • sporadic incidence of patches of pigmented hair around ears and eyes or rump
  • irregular coat pigmentation (MGI Ref ID J:6571)
    • mosaicism for large, sharply defined pigmented area
    • attributed to heterogeneity for wild-type allele
  • hematopoietic system phenotype
  • macrocytic anemia (MGI Ref ID J:6571)
    • mild, normochromic, macrocytic anemia
  • skin/coat/nails phenotype
  • absent coat pigmentation (MGI Ref ID J:6571)
    • white coat with some dark pigmentation on the ears
    • sporadic incidence of patches of pigmented hair around ears and eyes or rump
  • irregular coat pigmentation (MGI Ref ID J:6571)
    • mosaicism for large, sharply defined pigmented area
    • attributed to heterogeneity for wild-type allele
  • endocrine/exocrine gland phenotype
  • abnormal ovarian follicle number (MGI Ref ID J:6571)
  • small testis (MGI Ref ID J:6571)

KitW-42J/KitW-42J

        involves: C57BL/6J
  • lethality-prenatal/perinatal
  • perinatal lethality (MGI Ref ID J:6571)
    • die late in gestation or immediately after birth
  • hematopoietic system phenotype
  • macrocytic anemia (MGI Ref ID J:6571)

KitW-42J/KitW-42J

        C57BL/6J-KitW-42J
  • lethality-prenatal/perinatal
  • embryonic lethality during organogenesis (MGI Ref ID J:36829)
    • at E12.5, sub-Mendelian ratios are recovered
  • reproductive system phenotype
  • *normal* reproductive system phenotype (MGI Ref ID J:36829)
    • testes differentiation is not delayed

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

KitW-42J/Kit+

        involves: C57BL/6 * C57BL/6J * WB
  • skin/coat/nails phenotype
  • diluted coat color (MGI Ref ID J:27513)
  • white spotting (MGI Ref ID J:27513)
  • immune system phenotype
  • decreased mast cell number (MGI Ref ID J:27513)
    • mice exhibit fewer mast cells in the skin than wild-type mice
  • pigmentation phenotype
  • diluted coat color (MGI Ref ID J:27513)
  • white spotting (MGI Ref ID J:27513)
  • hematopoietic system phenotype
  • decreased erythrocyte cell number (MGI Ref ID J:27513)
  • decreased mast cell number (MGI Ref ID J:27513)
    • mice exhibit fewer mast cells in the skin than wild-type mice
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

KitW-42J related

Cancer Research
Growth Factors/Receptors/Cytokines
Increased Tumor Incidence (Gonadal Tumors: ovarian)
Oncogenes

Dermatology Research
Color and White Spotting Defects

Developmental Biology Research
Neural Crest Defects

Endocrine Deficiency Research
Bone/Bone Marrow Defects
Gonad Defects
Skin Defects

Immunology and Inflammation Research
Immunodeficiency (Mast Cell Deficiency)

Mouse/Human Gene Homologs
piebaldism

Neurobiology Research
Receptor Defects
Vestibular and Hearing Defects

Reproductive Biology Research
Developmental Defects Affecting Gonads (germ cell deficient)
Fertility Defects
Gonadal Tumors (ovarian)

Research Tools
Immunology and Inflammation Research (Mast Cell Deficiency)

Sensorineural Research
Vestibular and Hearing Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol KitW-42J
Allele Name dominant spotting 42 Jackson
Allele Type Spontaneous
Common Name(s) W-42; We;
Strain of OriginC57BL/6J
Gene Symbol and Name Kit, kit oncogene
Chromosome 5
Gene Common Name(s) Bs; C-Kit; CD117; Dominant white spotting; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; PBT; SCFR; SCO1; SCO5; SOW3; Ssm; Steel Factor Receptor; Tr-kit; W; belly-spot; dominant spotting; gsf spotted coat 1; gsf spotted coat 5; phenotype like Sl or W 3; spotted sterile male;
Molecular Note Nucleotide sequence analysis of mutant complementary DNAs revealed a G to A transition (position 2369) resulting in a missense mutation. Kit receptor tyrosine kinase activity is affected but not c-kit encoded proteins. [MGI Ref ID J:10187]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Additional References

Arguello F; Furlanetto RW; Baggs RB; Graves BT; Harwell SE; Cohen HJ; Frantz CN. 1992. Incidence and distribution of experimental metastases in mutant mice with defective organ microenvironments (genotypes Sl/Sld and W/Wv). Cancer Res 52(8):2304-9. [PubMed: 1559233]  [MGI Ref ID J:468]

Kitamura Y; Kasugai T; Ebi Y; Nomura S. 1991. Fibroblast-dependent differentiation/proliferation of mast cells. Skin Pharmacol 4 Suppl 1:2-7. [PubMed: 1764248]  [MGI Ref ID J:619]

Murphy ED. 1972. Hyperplastic and early neoplastic changes in the ovaries of mice after genic deletion of germ cells. J Natl Cancer Inst 48(5):1283-95. [PubMed: 4337905]  [MGI Ref ID J:5274]

Nocka K; Tan JC; Chiu E; Chu TY; Ray P; Traktman P; Besmer P. 1990. Molecular bases of dominant negative and loss of function mutations at the murine c-kit/white spotting locus: W37, Wv, W41 and W. EMBO J 9(6):1805-13. [PubMed: 1693331]  [MGI Ref ID J:10528]

KitW-42J related

Geissler EN; McFarland EC; Russell ES. 1981. Analysis of pleiotropism at the dominant white-spotting (W) locus of the house mouse: a description of ten new W alleles. Genetics 97(2):337-61. [PubMed: 7274658]  [MGI Ref ID J:6571]

Nagamine CM; Capehart J; Carlisle C; Chang D. 1998. Ovotestes in B6-XXSxr sex-reversed mice. Dev Biol 196(1):24-32. [PubMed: 9527878]  [MGI Ref ID J:47046]

Nagamine CM; Carlisle C. 1996. The dominant white spotting oncogene allele Kit(W-42J) exacerbates XY(DOM) sex reversal. Development 122(11):3597-605. [PubMed: 8951075]  [MGI Ref ID J:36829]

Sharma Y; Astle CM; Harrison DE. 2007. Heterozygous kit mutants with little or no apparent anemia exhibit large defects in overall hematopoietic stem cell function. Exp Hematol 35(2):214-220. [PubMed: 17258070]  [MGI Ref ID J:123190]

Tan JC; Nocka K; Ray P; Traktman P; Besmer P. 1990. The dominant W42 spotting phenotype results from a missense mutation in the c-kit receptor kinase. Science 247(4939):209-12. [PubMed: 1688471]  [MGI Ref ID J:10187]

Tsujimura T; Koshimizu U; Katoh H; Isozaki K; Kanakura Y; Tono T; Adachi S; Kasugai T; Tei H; Nishimune Y; Nomura S; Kitamura Y. 1993. Mast cell number in the skin of heterozygotes reflects the molecular nature of c-kit mutation. Blood 81(10):2530-8. [PubMed: 7683920]  [MGI Ref ID J:27513]

Wilson MJ; Bowles J; Koopman P. 2006. The matricellular protein SPARC is internalized in Sertoli, Leydig, and germ cells during testis differentiation. Mol Reprod Dev 73(5):531-9. [PubMed: 16425238]  [MGI Ref ID J:109014]

Wilson MJ; Liaw L; Koopman P. 2005. Osteopontin and related SIBLING glycoprotein genes are expressed by Sertoli cells during mouse testis development. Dev Dyn 233(4):1488-95. [PubMed: 15937924]  [MGI Ref ID J:99574]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $1900.00
*Price(s) in US dollars ($)

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $2470.00
*Price(s) in US dollars ($)

Additional Supply Details

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions


See Terms of Use


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries

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phone:207-288-6470
fax:207-288-6655

JAX® Mice & Services Conditions of Use

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