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Strain Name:

B6.D2-KitlSl-d/J

Stock Number:

000160

Availability:

Repository- Live

Price and Supply Information

General Terms and Conditions

Genes & Alleles   Kitl;   KitlSl-d;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Mating SystemOther - see Strain Mating Scheme Text         (Female x Male)
Heterozygote x C57BL/6J or C57BL/6J x heterozygote
Specieslaboratory mouse
Background Strain C57BL/6J
Donor Strain DBA/2J
GenerationN76 (09-AUG-07)

Appearance
grey with light belly, infrequent belly spot, affected
Related Genotype: a/a KitlSl-d/+

black, unaffected
Related Genotype: a/a +/+

Strain Description
The multiple steel mutations (KitlSl) behave in a semidominant fashion and cause deficiencies in pigment cells, germ cells, and blood cells paralleling those caused by the Kit locus mutations (dominant spotting alleles). Most of the alleles at steel locus cause severe anemia in utero and death by 15 to 16 days of gestation in homozygous mutant mice. However, steel-Dickie homozygotes (KitlSl-d/KitlSl-d) and compounds of steel and steel Dickie (KitlSl/KitlSl-d) are viable, black-eyed white, are usually sterile in one or both sexes, and have severe macrocytic anemia. Heterozygous steel mice have a diluted coat color with a small amount of white spotting, are viable and fertile, and may have a slight macrocytic anemia. Primordial germ cells are absent in the nonviable steel homozygotes and severely reduced in steel Dickie and steel heterozygotes. Mast cells are virtually absent in skin and other tissues of steel mutant mice. Tumors tend to develop in germ-cell-deficient ovaries with advancing age.

Although homozygous KitlSl-d/KitlSL-d have been described as being viable, Dr. Jane Barker of The Jackson Laboratory has found that in her colony B6.D2-KitlSl-d homozygotes die at birth or soon thereafter.

Mammalian Phenotype Terms assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

KitlSl-d/Kitl+

        either: DBA/2J or (C57BL/6 x DBA/2)F1
  • pigmentation phenotype
  • diluted coat color (MGI Ref ID J:13392)
    • heterozygotes have a slightly diluted coat color
  • skin/coat/nails phenotype
  • diluted coat color (MGI Ref ID J:13392)
    • heterozygotes have a slightly diluted coat color

KitlSl-d/Kitl+

        either: (involves: C57BL/6 * DBA/2J) or (involves: C3H * C57BL/6 * DBA/2J * WC)
  • hematopoietic system phenotype
  • anemia (MGI Ref ID J:6084)
    • mice are slightly anemic
  • decreased mast cell number (MGI Ref ID J:6084)
    • heterozygotes have decreased mast cell numbers in dorsal skin compared to wild type
  • immune system phenotype
  • decreased mast cell number (MGI Ref ID J:6084)
    • heterozygotes have decreased mast cell numbers in dorsal skin compared to wild type

KitlSl-d/Kitl+

        C3.D2-KitlSl-d
  • hematopoietic system phenotype
  • increased mean corpuscular hemoglobin (MGI Ref ID J:79293)
    • significantly increased compared to wild-type at P24-25
  • increased mean corpuscular volume (MGI Ref ID J:79293)
    • significantly increased compared to wild-type at P24-25
  • low mean erythrocyte cell number (MGI Ref ID J:79293)
    • at P1, mean red blood cell (RBC) counts are not different from KitlSl-d / KitlSl-gb compound heterozygotes (2.9 x 109 cells/ml; 4.1 x 109 cells/ml in wild-type mice)
  • macrocytic anemia (MGI Ref ID J:79293)
    • mild at birth
  • pigmentation phenotype
  • abnormal ventral coat pigmentation (MGI Ref ID J:79293)
    • diluted ventrum
  • head spot (MGI Ref ID J:79293)
  • skin/coat/nails phenotype
  • abnormal ventral coat pigmentation (MGI Ref ID J:79293)
    • diluted ventrum
  • head spot (MGI Ref ID J:79293)

KitlSl-d/KitlSl-d

        either: DBA/2J or (C57BL/6 x DBA/2)F1
  • hematopoietic system phenotype
  • anemia (MGI Ref ID J:20286)
    • mice display anemia
  • pigmentation phenotype
  • abnormal coat color (MGI Ref ID J:20286)
    • mice are white (with black eyes)
  • reproductive system phenotype
  • infertility (MGI Ref ID J:20286)
    • mice are sterile
  • skin/coat/nails phenotype
  • abnormal coat color (MGI Ref ID J:20286)
    • mice are white (with black eyes)

KitlSl-d/KitlSl-d

        C3.D2-KitlSl-d
  • life span-post-weaning/aging
  • *normal* life span-post-weaning/aging (MGI Ref ID J:79293)
    • homozygotes are viable with expected number of homozygotes observed at P1; 83% of mice survive to P18, similar to wild-type
  • hematopoietic system phenotype
  • decreased hematocrit (MGI Ref ID J:79293)
    • significantly lower than wild-type at P24-25
  • increased mean corpuscular hemoglobin concentration (MGI Ref ID J:79293)
    • significantly increased compared to wild-type at P24-25
  • increased mean corpuscular volume (MGI Ref ID J:79293)
    • significantly increased compared to wild-type at P24-25
  • low mean erythrocyte cell number (MGI Ref ID J:79293)
    • significantly lower than wild-type at birth (32% of wild-type value)
  • macrocytic anemia (MGI Ref ID J:79293)
    • severe at birth
  • reproductive system phenotype
  • abnormal primordial germ cell morphology (MGI Ref ID J:115437)
    • between E9.5 and 10.5, most PGCs are found with in the hindgut and these have abnormal morphology, while in wild-type embryos most PGCs are found in dorsal portions of mesentery
    • decreased primordial germ cell number (MGI Ref ID J:115437)
      • moderate numbers of primordial germ cells (PGCs) are seen in genital ridges relative to wild-type and KitlSl-gb homozygotes at E11.5
      • at E9.5, PGCs are located primarily in the ventral axis of the hindgut while in wild-type PGCs are found primarily associated with the hindgut epithelium or in the dorsal axis of the hindgut; total PGC number in mutant embryos is 22% of wild-type
  • cellular phenotype
  • abnormal cell migration (MGI Ref ID J:115437)
    • at E10.5, only 45% of total PGCs have migrated from hindgut, compared to 93% in wild-type
  • abnormal cell proliferation (MGI Ref ID J:115437)
    • proliferation indices of migratory (in mesentery and genital ridges) and postmigratory PGCs (in genital ridges) at 10.5 and 11.5 are significantly reduced compared to wild-type values (54-66% of wild-type values)
  • increased apoptosis (MGI Ref ID J:115437)
    • at E10.5, many PGCs in hindgut appear to be disintegrating; abnormal PGCs in hindgut tend to be nonmotile and apoptotic

Gene & Allele Details

Allele Symbol KitlSl-d
Allele Name steel Dickie
Common Name(s) KL; MGF; MgfSl-d; Sld; Sld;
Strain of OriginDBA/2J
Gene Symbol and Name Kitl, kit ligand
Chromosome 10
Gene Common Name(s) Clo; Con; DKFZp686F2250; Gb; KL-1; Kl1; Kl2; MGF; Mgf; SCF; SF; SHEP7; SLF; Sl; Steel; Steel factor; cloud gray; contrasted; grizzle-belly; mast cell growth factor; steel; stem cell factor;
General Note

Genbank ID for this allele: M64262

Molecular Note A 4kb deletion in genomic DNA results in the absence of 241bp of wild type cDNA and the addition of 67bp of novel sequence, a 174bp net loss. The region that is deleted begins 5 amino acids N-terminal to the transmembrane domain and results in termination of the open reading frame after an additional 3 amino acids. The resulting protein is a soluble truncated one, lacking both transmembrane and cytoplasmic domains. Northern analysis indicates that mRNA is transcribed at nearly wild-type levels in adult tiss [MGI Ref ID J:10750] [MGI Ref ID J:20286] [MGI Ref ID J:40339]

Control Information

  Allele   Control
 KitlSl-d  Wild-type from the colony
 KitlSl-d  000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for JAX® GEMM® Strains

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

Related Strains

Strains carrying   KitlSl-d allele
000161   WB.D2-KitlSl-d/J
100401   WCB6F1/J KitlSl KitlSl-d
View Strains carrying   KitlSl-d     (2 strains)

View Strains carrying other alleles of Kitl     (10 strains)

Additional Web Information

Congenic Nomenclature
JAX® NOTES, February 2001; 481. Mgf Gene Name Changes to Kitl.

Animal Health Reports

Room Number           FGB27

Research Applications

This mouse can be used to support research in many areas including:

Mouse/Human Gene Homologs
synpolydactyly

KitlSl-d related

Cancer Research
Growth Factors/Receptors/Cytokines
Increased Tumor Incidence (Gonadal Tumors: ovarian and testicular)

Dermatology Research
Color and White Spotting Defects

Developmental Biology Research
Neural Tube Defects

Endocrine Deficiency Research
Bone/Bone Marrow Defects
Gonad Defects
Hypothalamus/Pituitary Defects
Skin Defects

Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Immunodeficiency (Mast Cell Deficiency)

Neurobiology Research
Vestibular and Hearing Defects

Reproductive Biology Research
Developmental Defects Affecting Gonads (germ cell deficient)
Fertility Defects
Gonadal Tumors (ovarian and testicular)

Research Tools
Immunology and Inflammation Research (Mast Cell Deficiency)

Sensorineural Research
Vestibular and Hearing Defects

References

Selected Reference(s)

Arguello F; Furlanetto RW; Baggs RB; Graves BT; Harwell SE; Cohen HJ; Frantz CN. 1992. Incidence and distribution of experimental metastases in mutant mice with defective organ microenvironments (genotypes Sl/Sld and W/Wv). Cancer Res 52(8):2304-9. [PubMed: 1559233]  [MGI Ref ID J:468]

Hayashi C; Sonoda T; Nakano T; Nakayama H; Kitamura Y. 1985. Mast-cell precursors in the skin of mouse embryos and their deficiency in embryos of Sl/Sld genotype. Dev Biol 109(1):234-41. [PubMed: 3987963]  [MGI Ref ID J:7810]

Huang E; Nocka K; Beier DR; Chu TY; Buck J; Lahm HW; Wellner D; Leder P; Besmer P. 1990. The hematopoietic growth factor KL is encoded by the Sl locus and is the ligand of the c-kit receptor, the gene product of the W locus. Cell 63(1):225-33. [PubMed: 1698557]  [MGI Ref ID J:10751]

Murphy ED. 1977. Effects of mutant steel alleles on leukemogenesis and life-span in the mouse. J Natl Cancer Inst 58(1):107-10. [PubMed: 319242]  [MGI Ref ID J:5758]

Shinohara T; Avarbock MR; Brinster RL. 2000. Functional analysis of spermatogonial stem cells in Steel and cryptorchid infertile mouse models. Dev Biol 220(2):401-11. [PubMed: 10753526]  [MGI Ref ID J:61712]

Zsebo KM; Williams DA; Geissler EN; Broudy VC; Martin FH; Atkins HL; Hsu RY; Birkett NC; Okino KH; Murdock DC; Jacobsen FW; Langley KE; Smith KA; Takeishi T; Cattanach BM; Galli SJ; Suggs SV. 1990. Stem cell factor is encoded at the Sl locus of the mouse and is the ligand for the c-kit tyrosine kinase receptor. Cell 63(1):213-24. [PubMed: 1698556]  [MGI Ref ID J:10750]

Additional References

Price and Supply Information

Strain Name: B6.D2-KitlSl-d/J
Stock Number: 000160

Price Details

IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:

*Pricing for Shipping Destination selected:

        USA, Canada and Mexico

Price(s) in US dollars ($)Genotype(s) Provided
Individual Mouse Price $118.30Heterozygous for KitlSl-d
Pair $170.55Heterozygous for KitlSl-d x Wild-type for KitlSl-d
Pair $170.55Wild-type for KitlSl-d x Heterozygous for KitlSl-d

Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-800-422-6423.
Usually shipped between four and eight weeks of age.
This strain is included in the Mouse Mutant Resource collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.
LicensingSee General Terms and Conditions below  
Control InformationView Control Information in Strain Details.
View Control Pricing Information for JAX® Strains.

General Terms and Conditions

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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