Strain Name:

WB.D2-KitlSl-d/J

Stock Number:

000161

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names MgfSl-di    (Changed: 15-DEC-04 )
Type Congenic; Mutant Strain;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain WB/Re
Donor Strain DBA/2J
GenerationN42
Generation Definitions

Description
The multiple steel mutations (KitlSl) behave in a semidominant fashion and cause deficiencies in pigment cells, germ cells, and blood cells paralleling those caused by the Kit locus mutations (dominant spotting alleles). Most of the alleles at steel locus cause severe anemia in utero and death by 15 to 16 days of gestation in homozygous mutant mice. However, steel-Dickie homozygotes (KitlSl-d/KitlSl-d) and compounds of steel and steel Dickie (KitlSl/KitlSl-d) are viable, black-eyed white, are usually sterile in one or both sexes, and have severe macrocytic anemia. Heterozygous steel mice have a diluted coat color with a small amount of white spotting, are viable and fertile, and may have a slight macrocytic anemia. Primordial germ cells are absent in the nonviable steel homozygotes and severely reduced in steel Dickie and steel heterozygotes. Mast cells are virtually absent in skin and other tissues of steel mutant mice. Tumors tend to develop in germ-cell-deficient ovaries with advancing age.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that thephenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   KitlSl-d allele
000160   B6.D2-KitlSl-d/J
View Strains carrying   KitlSl-d     (1 strain)

View Strains carrying other alleles of Kitl     (14 strains)

Additional Web Information

JAX® NOTES, February 2001; 481. Mgf Gene Name Changes to Kitl.

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Hyperpigmentation, Familial Progressive, 2; FPH2   (KITLG)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

KitlSl-d/Kitl+

        either: DBA/2J or (C57BL/6 x DBA/2)F1
  • pigmentation phenotype
  • diluted coat color
    • heterozygotes have a slightly diluted coat color   (MGI Ref ID J:13392)
  • integument phenotype
  • diluted coat color
    • heterozygotes have a slightly diluted coat color   (MGI Ref ID J:13392)

KitlSl-d/Kitl+

        either: (involves: C57BL/6 * DBA/2J) or (involves: C3H * C57BL/6 * DBA/2J * WC)
  • hematopoietic system phenotype
  • anemia
    • mice are slightly anemic   (MGI Ref ID J:6084)
  • decreased mast cell number
    • heterozygotes have decreased mast cell numbers in dorsal skin compared to wild-type   (MGI Ref ID J:6084)
  • immune system phenotype
  • decreased mast cell number
    • heterozygotes have decreased mast cell numbers in dorsal skin compared to wild-type   (MGI Ref ID J:6084)

KitlSl-d/Kitl+

        C3.D2-KitlSl-d
  • hematopoietic system phenotype
  • increased mean corpuscular hemoglobin
    • significantly increased compared to wild-type at P24-25   (MGI Ref ID J:79293)
  • increased mean corpuscular volume
    • significantly increased compared to wild-type at P24-25   (MGI Ref ID J:79293)
  • low mean erythrocyte cell number
    • at P1, mean red blood cell (RBC) counts are not different from KitlSl-d / KitlSl-gb compound heterozygotes (2.9 x 109 cells/ml; 4.1 x 109 cells/ml in wild-type mice)   (MGI Ref ID J:79293)
  • macrocytic anemia
    • mild at birth   (MGI Ref ID J:79293)
  • pigmentation phenotype
  • abnormal ventral coat pigmentation
    • diluted ventrum   (MGI Ref ID J:79293)
  • head spot   (MGI Ref ID J:79293)
  • integument phenotype
  • abnormal ventral coat pigmentation
    • diluted ventrum   (MGI Ref ID J:79293)
  • head spot   (MGI Ref ID J:79293)

KitlSl-d/Kitl+

        involves: DBA/2J
  • hematopoietic system phenotype
  • macrocytic anemia
    • mild macrocytic anemia   (MGI Ref ID J:125080)
  • pigmentation phenotype
  • diluted coat color
    • slightly diluted coat color is more noticeable on the belly   (MGI Ref ID J:125080)
  • integument phenotype
  • diluted coat color
    • slightly diluted coat color is more noticeable on the belly   (MGI Ref ID J:125080)

KitlSl-d/KitlSl-d

        Background Not Specified
  • embryogenesis phenotype
  • abnormal melanoblast morphology
    • at embryonic day 11 there are far fewer than normal melanoblasts and these are mostly restricted to a site just caudal to the otic visicle, from embryonic day 12 onward very few melanoblasts are found, and none are found postnatally   (MGI Ref ID J:31646)
  • nervous system phenotype
  • abnormal melanoblast morphology
    • at embryonic day 11 there are far fewer than normal melanoblasts and these are mostly restricted to a site just caudal to the otic visicle, from embryonic day 12 onward very few melanoblasts are found, and none are found postnatally   (MGI Ref ID J:31646)

KitlSl-d/KitlSl-d

        either: DBA/2J or (C57BL/6 x DBA/2)F1
  • hematopoietic system phenotype
  • anemia   (MGI Ref ID J:20286)
    • mice display anemia   (MGI Ref ID J:13392)
  • pigmentation phenotype
  • abnormal coat/hair pigmentation   (MGI Ref ID J:20286)
    • mice are white (with black eyes)   (MGI Ref ID J:13392)
  • reproductive system phenotype
  • infertility   (MGI Ref ID J:20286)
    • mice are sterile   (MGI Ref ID J:13392)
  • integument phenotype
  • abnormal coat/hair pigmentation   (MGI Ref ID J:20286)
    • mice are white (with black eyes)   (MGI Ref ID J:13392)

KitlSl-d/KitlSl-d

        C3.D2-KitlSl-d
  • mortality/aging
  • *normal* mortality/aging
    • homozygotes are viable with expected number of homozygotes observed at P1; 83% of mice survive to P18, similar to wild-type   (MGI Ref ID J:79293)
  • hematopoietic system phenotype
  • decreased hematocrit
    • significantly lower than wild-type at P24-25   (MGI Ref ID J:79293)
  • increased mean corpuscular hemoglobin concentration
    • significantly increased compared to wild-type at P24-25   (MGI Ref ID J:79293)
  • increased mean corpuscular volume
    • significantly increased compared to wild-type at P24-25   (MGI Ref ID J:79293)
  • low mean erythrocyte cell number
    • significantly lower than wild-type at birth (32% of wild-type value)   (MGI Ref ID J:79293)
  • macrocytic anemia
    • severe at birth   (MGI Ref ID J:79293)
  • reproductive system phenotype
  • abnormal primordial germ cell migration
    • at E10.5, only 45% of total PGCs have migrated from hindgut, compared to 93% in wild-type   (MGI Ref ID J:115437)
  • abnormal primordial germ cell morphology
    • between E9.5 and 10.5, most PGCs are found with in the hindgut and these have abnormal morphology, while in wild-type embryos most PGCs are found in dorsal portions of mesentery   (MGI Ref ID J:115437)
    • decreased primordial germ cell number
      • moderate numbers of primordial germ cells (PGCs) are seen in genital ridges relative to wild-type and KitlSl-gb homozygotes at E11.5   (MGI Ref ID J:115437)
      • at E9.5, PGCs are located primarily in the ventral axis of the hindgut while in wild-type PGCs are found primarily associated with the hindgut epithelium or in the dorsal axis of the hindgut; total PGC number in mutant embryos is 22% of wild-type   (MGI Ref ID J:115437)
  • abnormal primordial germ cell proliferation
    • proliferation indices of migratory (in mesentery and genital ridges) and postmigratory PGCs (in genital ridges) at 10.5 and 11.5 are significantly reduced compared to wild-type values (54-66% of wild-type values)   (MGI Ref ID J:115437)
  • cellular phenotype
  • abnormal primordial germ cell migration
    • at E10.5, only 45% of total PGCs have migrated from hindgut, compared to 93% in wild-type   (MGI Ref ID J:115437)
  • abnormal primordial germ cell proliferation
    • proliferation indices of migratory (in mesentery and genital ridges) and postmigratory PGCs (in genital ridges) at 10.5 and 11.5 are significantly reduced compared to wild-type values (54-66% of wild-type values)   (MGI Ref ID J:115437)
  • increased apoptosis
    • at E10.5, many PGCs in hindgut appear to be disintegrating; abnormal PGCs in hindgut tend to be nonmotile and apoptotic   (MGI Ref ID J:115437)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

KitlSl-d related

Cancer Research
Growth Factors/Receptors/Cytokines
Increased Tumor Incidence
      Gonadal Tumors
      Gonadal Tumors: ovarian and testicular

Dermatology Research
Color and White Spotting Defects

Developmental Biology Research
Neural Tube Defects

Endocrine Deficiency Research
Bone/Bone Marrow Defects
Gonad Defects
Hypothalamus/Pituitary Defects
Skin Defects

Immunology, Inflammation and Autoimmunity Research
Growth Factors/Receptors/Cytokines
Immunodeficiency
      Mast Cell Deficiency

Neurobiology Research
Hearing Defects

Reproductive Biology Research
Developmental Defects Affecting Gonads
      germ cell deficient
Fertility Defects
Gonadal Tumors
      ovarian and testicular

Research Tools
Immunology, Inflammation and Autoimmunity Research
      Mast Cell Deficiency

Sensorineural Research
Hearing Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol KitlSl-d
Allele Name steel Dickie
Allele Type Spontaneous
Common Name(s) KL; MGF; MgfSl-d; Sld; Sld; W/Wv;
Strain of OriginDBA/2J
Gene Symbol and Name Kitl, kit ligand
Chromosome 10
Gene Common Name(s) Clo; Con; FPH2; Gb; KL-1; MGF; Mgf; SCF; SF; SHEP7; SLF; Sl; Steel; Steel factor; blaze; blz; cloud gray; contrasted; grizzle-belly; mast cell growth factor; steel; stem cell factor;
General Note

Genbank ID for this allele: M64262

Molecular Note A 4kb deletion in genomic DNA results in the absence of 241bp in the mRNA and the addition of 67bp of novel sequence, a 174bp net loss. The region that is deleted corresponds to 5 amino acids N-terminal to the transmembrane domain of the encoded protein, and results in termination of the open reading frame after an additional 3 amino acids. The resulting protein is a soluble truncated one, lacking both transmembrane and cytoplasmic domains. Northern analysis indicates that mRNA is transcribed at nearly wild-type levels in adult tissues. [MGI Ref ID J:10750] [MGI Ref ID J:20286] [MGI Ref ID J:40339]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Arguello F; Furlanetto RW; Baggs RB; Graves BT; Harwell SE; Cohen HJ; Frantz CN. 1992. Incidence and distribution of experimental metastases in mutant mice with defective organ microenvironments (genotypes Sl/Sld and W/Wv). Cancer Res 52(8):2304-9. [PubMed: 1559233]  [MGI Ref ID J:468]

Hayashi C; Sonoda T; Nakano T; Nakayama H; Kitamura Y. 1985. Mast-cell precursors in the skin of mouse embryos and their deficiency in embryos of Sl/Sld genotype. Dev Biol 109(1):234-41. [PubMed: 3987963]  [MGI Ref ID J:7810]

Huang E; Nocka K; Beier DR; Chu TY; Buck J; Lahm HW; Wellner D; Leder P; Besmer P. 1990. The hematopoietic growth factor KL is encoded by the Sl locus and is the ligand of the c-kit receptor, the gene product of the W locus. Cell 63(1):225-33. [PubMed: 1698557]  [MGI Ref ID J:10751]

Murphy ED. 1977. Effects of mutant steel alleles on leukemogenesis and life-span in the mouse. J Natl Cancer Inst 58(1):107-10. [PubMed: 319242]  [MGI Ref ID J:5758]

Schrott A; Egg G; Spoendlin H. 1988. Intermediate filaments in the cochleas of normal and mutant (w/wv, sl/sld) mice. Arch Otorhinolaryngol 245(4):250-4. [PubMed: 2460075]  [MGI Ref ID J:9423]

Schrott A; Spoendlin H. 1987. Pigment anomaly-associated inner ear deafness. Acta Otolaryngol (Stockh) 103(5-6):451-7. [PubMed: 3618172]  [MGI Ref ID J:8813]

Wolf NS. 1978. Dissecting the hematopoietic microenvironment. II. The kinetics of the erythron of the S1/S1d mouse and the dual nature of its anemia. Cell Tissue Kinet 11(4):325-34. [PubMed: 688326]  [MGI Ref ID J:6031]

Zsebo KM; Williams DA; Geissler EN; Broudy VC; Martin FH; Atkins HL; Hsu RY; Birkett NC; Okino KH; Murdock DC; Jacobsen FW; Langley KE; Smith KA; Takeishi T; Cattanach BM; Galli SJ; Suggs SV. 1990. Stem cell factor is encoded at the Sl locus of the mouse and is the ligand for the c-kit tyrosine kinase receptor. Cell 63(1):213-24. [PubMed: 1698556]  [MGI Ref ID J:10750]

KitlSl-d related

Barker JE. 1997. Early transplantation to a normal microenvironment prevents the development of Steel hematopoietic stem cell defects. Exp Hematol 25(6):542-7. [PubMed: 9197334]  [MGI Ref ID J:41120]

Beckett EA; Horiguchi K; Khoyi M; Sanders KM; Ward SM. 2002. Loss of enteric motor neurotransmission in the gastric fundus of Sl/Sl(d) mice. J Physiol 543(Pt 3):871-87. [PubMed: 12231645]  [MGI Ref ID J:105989]

Bernstein S. 1960. Steel Dickie Mouse News Lett 23:33-4.  [MGI Ref ID J:13392]

Brannan CI; Lyman SD; Williams DE; Eisenman J; Anderson DM; Cosman D; Bedell MA; Jenkins NA; Copeland NG. 1991. Steel-Dickie mutation encodes a c-kit ligand lacking transmembrane and cytoplasmic domains. Proc Natl Acad Sci U S A 88(11):4671-4. [PubMed: 1711207]  [MGI Ref ID J:20286]

Chen R; Fairley JA; Zhao ML; Giudice GJ; Zillikens D; Diaz LA; Liu Z. 2002. Macrophages, but not T and B lymphocytes, are critical for subepidermal blister formation in experimental bullous pemphigoid: macrophage-mediated neutrophil infiltration depends on mast cell activation. J Immunol 169(7):3987-92. [PubMed: 12244200]  [MGI Ref ID J:120403]

Chen R; Ning G; Zhao ML; Fleming MG; Diaz LA; Werb Z; Liu Z. 2001. Mast cells play a key role in neutrophil recruitment in experimental bullous pemphigoid. J Clin Invest 108(8):1151-8. [PubMed: 11602622]  [MGI Ref ID J:72195]

Choi Y; Rajkovic A. 2006. Genetics of early mammalian folliculogenesis. Cell Mol Life Sci 63(5):579-90. [PubMed: 16416028]  [MGI Ref ID J:108336]

Deol MS. 1970. The relationship between abnormalities of pigmentation and of the inner ear. Proc R Soc Lond B Biol Sci 175(39):201-17. [PubMed: 4392283]  [MGI Ref ID J:125080]

Ding H; Nedrud JG; Wershil B; Redline RW; Blanchard TG; Czinn SJ. 2009. Partial protection against Helicobacter pylori in the absence of mast cells in mice. Infect Immun 77(12):5543-50. [PubMed: 19822650]  [MGI Ref ID J:155471]

Flanagan JG; Chan DC; Leder P. 1991. Transmembrane form of the kit ligand growth factor is determined by alternative splicing and is missing in the Sld mutant. Cell 64(5):1025-35. [PubMed: 1705866]  [MGI Ref ID J:40339]

Galli SJ; Hammel I. 1984. Unequivocal delayed hypersensitivity in mast cell-deficient and beige mice. Science 226(4675):710-3. [PubMed: 6494907]  [MGI Ref ID J:127346]

Gore BB; Wong KG; Tessier-Lavigne M. 2008. Stem cell factor functions as an outgrowth-promoting factor to enable axon exit from the midline intermediate target. Neuron 57(4):501-10. [PubMed: 18304480]  [MGI Ref ID J:132880]

Gu Y; Runyan C; Shoemaker A; Surani MA; Wylie C. 2011. Membrane-bound steel factor maintains a high local concentration for mouse primordial germ cell motility, and defines the region of their migration. PLoS One 6(10):e25984. [PubMed: 21998739]  [MGI Ref ID J:179622]

Gurish MF; Tao H; Abonia JP; Arya A; Friend DS; Parker CM; Austen KF. 2001. Intestinal mast cell progenitors require CD49dbeta7 (alpha4beta7 integrin) for tissue-specific homing. J Exp Med 194(9):1243-52. [PubMed: 11696590]  [MGI Ref ID J:119138]

Heissig B; Rafii S; Akiyama H; Ohki Y; Sato Y; Rafael T; Zhu Z; Hicklin DJ; Okumura K; Ogawa H; Werb Z; Hattori K. 2005. Low-dose irradiation promotes tissue revascularization through VEGF release from mast cells and MMP-9-mediated progenitor cell mobilization. J Exp Med 202(6):739-50. [PubMed: 16157686]  [MGI Ref ID J:107445]

Higuchi H; Hara M; Yamamoto K; Miyamoto T; Kinoshita M; Yamada T; Uchiyama K; Matsumori A. 2008. Mast cells play a critical role in the pathogenesis of viral myocarditis. Circulation 118(4):363-72. [PubMed: 18606918]  [MGI Ref ID J:155664]

Hu B; Colletti LM. 2008. Stem cell factor and c-kit are involved in hepatic recovery after acetaminophen-induced liver injury in mice. Am J Physiol Gastrointest Liver Physiol 295(1):G45-G53. [PubMed: 18467506]  [MGI Ref ID J:137545]

Huang EJ; Nocka KH; Buck J; Besmer P. 1992. Differential expression and processing of two cell associated forms of the kit-ligand: KL-1 and KL-2. Mol Biol Cell 3(3):349-62. [PubMed: 1378327]  [MGI Ref ID J:1527]

Ishii M; Tachiwana T; Hoshino A; Tsunekawa N; Hiramatsu R; Matoba S; Kanai-Azuma M; Kawakami H; Kurohmaru M; Kanai Y. 2007. Potency of testicular somatic environment to support spermatogenesis in XX/Sry transgenic male mice. Development 134(3):449-54. [PubMed: 17185318]  [MGI Ref ID J:135064]

Jones TG; Hallgren J; Humbles A; Burwell T; Finkelman FD; Alcaide P; Austen KF; Gurish MF. 2009. Antigen-induced increases in pulmonary mast cell progenitor numbers depend on IL-9 and CD1d-restricted NKT cells. J Immunol 183(8):5251-60. [PubMed: 19783672]  [MGI Ref ID J:153832]

Kato M; Takeda K; Kawamoto Y; Tsuzuki T; Hossain K; Tamakoshi A; Kunisada T; Kambayashi Y; Ogino K; Suzuki H; Takahashi M; Nakashima I. 2004. c-Kit-Targeting Immunotherapy for Hereditary Melanoma in a Mouse Model. Cancer Res 64(3):801-6. [PubMed: 14871802]  [MGI Ref ID J:88074]

Kitamura Y; Go S. 1979. Decreased production of mast cells in S1/S1d anemic mice. Blood 53(3):492-7. [PubMed: 367470]  [MGI Ref ID J:6084]

Kitamura Y; Yokoyama M; Matsuda H; Shimada M. 1980. Coincidental development of forestomach papilloma and prepyloric ulcer in nontreated mutant mice of W/Wv and SI/SId genotypes. Cancer Res 40(9):3392-7. [PubMed: 7000343]  [MGI Ref ID J:6393]

Kondoh G; Hayasaka N; Li Q; Nishimune Y; Hakura A. 1995. An in vivo model for receptor tyrosine kinase autocrine/paracrine activation: auto-stimulated KIT receptor acts as a tumor promoting factor in papillomavirus-induced tumorigenesis. Oncogene 10(2):341-7. [PubMed: 7530826]  [MGI Ref ID J:90316]

Kraneveld AD; van der Kleij HP; Kool M; van Houwelingen AH; Weitenberg AC; Redegeld FA; Nijkamp FP. 2002. Key role for mast cells in nonatopic asthma. J Immunol 169(4):2044-53. [PubMed: 12165531]  [MGI Ref ID J:120703]

Krishnamoorthy N; Oriss TB; Paglia M; Fei M; Yarlagadda M; Vanhaesebroeck B; Ray A; Ray P. 2008. Activation of c-Kit in dendritic cells regulates T helper cell differentiation and allergic asthma. Nat Med 14(5):565-73. [PubMed: 18454155]  [MGI Ref ID J:136704]

Lee DM; Friend DS; Gurish MF; Benoist C; Mathis D; Brenner MB. 2002. Mast cells: a cellular link between autoantibodies and inflammatory arthritis. Science 297(5587):1689-92. [PubMed: 12215644]  [MGI Ref ID J:78906]

Lotinun S; Evans GL; Turner RT; Oursler MJ. 2005. Deletion of membrane-bound steel factor results in osteopenia in mice. J Bone Miner Res 20(4):644-52. [PubMed: 15765184]  [MGI Ref ID J:111273]

Lourenssen S; Motro B; Bernstein A; Diamond J. 2000. Defects in sensory nerve numbers and growth in mutant Kit and Steel mice. Neuroreport 11(6):1159-65. [PubMed: 10817584]  [MGI Ref ID J:103680]

Mahakali Zama A; Hudson FP 3rd; Bedell MA. 2005. Analysis of hypomorphic KitlSl mutants suggests different requirements for KITL in proliferation and migration of mouse primordial germ cells. Biol Reprod 73(4):639-47. [PubMed: 15917341]  [MGI Ref ID J:115437]

McCoshen JA; McCallion DJ. 1975. A study of the primordial germ cells during their migratory phase in Steel mutant mice. Experientia 31(5):589-90. [PubMed: 1170085]  [MGI Ref ID J:5547]

Mikkelsen HB; Malysz J; Huizinga JD; Thuneberg L. 1998. Action potential generation, Kit receptor immunohistochemistry and morphology of steel-Dickie (Sl/Sld) mutant mouse small intestine. Neurogastroenterol Motil 10(1):11-26. [PubMed: 9507248]  [MGI Ref ID J:113054]

Miyamoto K; Kobayashi T; Hayashi Y; Zhang Y; Hara Y; Higashine M; Shiraishi A; Ohashi Y. 2012. Involvement of stem cell factor and c-kit in corneal wound healing in mice. Mol Vis 18:1505-15. [PubMed: 22736941]  [MGI Ref ID J:191601]

Moro K; Yamada T; Tanabe M; Takeuchi T; Ikawa T; Kawamoto H; Furusawa J; Ohtani M; Fujii H; Koyasu S. 2010. Innate production of T(H)2 cytokines by adipose tissue-associated c-Kit(+)Sca-1(+) lymphoid cells. Nature 463(7280):540-4. [PubMed: 20023630]  [MGI Ref ID J:156764]

Motro B; Wojtowicz JM; Bernstein A; van der Kooy D. 1996. Steel mutant mice are deficient in hippocampal learning but not long-term potentiation. Proc Natl Acad Sci U S A 93(5):1808-13. [PubMed: 8700840]  [MGI Ref ID J:32130]

Murphy ED. 1977. Effects of mutant steel alleles on leukemogenesis and life-span in the mouse. J Natl Cancer Inst 58(1):107-10. [PubMed: 319242]  [MGI Ref ID J:5758]

Ogawa T; Dobrinski I; Avarbock MR; Brinster RL. 2000. Transplantation of male germ line stem cells restores fertility in infertile mice [see comments] Nat Med 6(1):29-34. [PubMed: 10613820]  [MGI Ref ID J:59322]

Ohta H; Aizawa S; Nishimune Y. 2003. Functional Analysis of the p53 Gene in Apoptosis Induced by Heat Stress or Loss of Stem Cell Factor Signaling in Mouse Male Germ Cells. Biol Reprod 68(6):2249-54. [PubMed: 12606380]  [MGI Ref ID J:83572]

Ohta H; Tohda A; Nishimune Y. 2003. Proliferation and differentiation of spermatogonial stem cells in the w/wv mutant mouse testis. Biol Reprod 69(6):1815-21. [PubMed: 12890724]  [MGI Ref ID J:108583]

Ohta H; Yomogida K; Dohmae K; Nishimune Y. 2000. Regulation of proliferation and differentiation in spermatogonial stem cells: the role of c-kit and its ligand SCF Development 127(10):2125-31. [PubMed: 10769236]  [MGI Ref ID J:61520]

Perez-Losada J; Sanchez-Martin M; Rodriguez-Garcia A; Sanchez ML; Orfao A; Flores T; Sanchez-Garcia I. 2002. Zinc-finger transcription factor Slug contributes to the function of the stem cell factor c-kit signaling pathway. Blood 100(4):1274-86. [PubMed: 12149208]  [MGI Ref ID J:78323]

Puddington L; Olson S; Lefrancois L. 1994. Interactions between stem cell factor and c-Kit are required for intestinal immune system homeostasis. Immunity 1(9):733-9. [PubMed: 7534619]  [MGI Ref ID J:189422]

Rajaraman S; Davis WS; Mahakali-Zama A; Evans HK; Russell LB; Bedell MA. 2002. An Allelic Series of Mutations in the Kit ligand Gene of Mice. II. Effects of Ethylnitrosourea-Induced Kitl Point Mutations on Survival and Peripheral Blood Cells of Kitl(Steel) Mice. Genetics 162(1):341-53. [PubMed: 12242245]  [MGI Ref ID J:79293]

Ren X; Hogaboam C; Carpenter A; Colletti L. 2003. Stem cell factor restores hepatocyte proliferation in IL-6 knockout mice following 70% hepatectomy. J Clin Invest 112(9):1407-18. [PubMed: 14597766]  [MGI Ref ID J:118475]

Russell ES. 1970. Abnormalities of erythropoiesis associated with mutant genes in mice. In: Regulation of Hematopoiesis. Appleton-Century-Crofts, New York.  [MGI Ref ID J:27511]

Russell ES; Bernstein SE. 1966. Blood and Blood Formation. In: Biology of the Laboratory Mouse. McGraw Hill, New York.  [MGI Ref ID J:24829]

Sarna SK. 2008. Are interstitial cells of Cajal plurifunction cells in the gut? Am J Physiol Gastrointest Liver Physiol 294(2):G372-90. [PubMed: 17932226]  [MGI Ref ID J:132097]

Sassa S; Bernstein SE. 1978. Studies of erythrocyte protoporphyrin in anemic mutant mice: use of a modified hematofluorometer for the detection of heterozygotes for hemolytic disease. Exp Hematol 6(5):479-87. [PubMed: 658175]  [MGI Ref ID J:5985]

Sato T; Yokonishi T; Komeya M; Katagiri K; Kubota Y; Matoba S; Ogonuki N; Ogura A; Yoshida S; Ogawa T. 2012. Testis tissue explantation cures spermatogenic failure in c-Kit ligand mutant mice. Proc Natl Acad Sci U S A 109(42):16934-8. [PubMed: 22984182]  [MGI Ref ID J:190116]

Schwarzenberger P; Huang W; Ye P; Oliver P; Manuel M; Zhang Z; Bagby G; Nelson S; Kolls JK. 2000. Requirement of endogenous stem cell factor and granulocyte-colony-stimulating factor for IL-17-mediated granulopoiesis. J Immunol 164(9):4783-9. [PubMed: 10779785]  [MGI Ref ID J:112156]

Shinohara T; Avarbock MR; Brinster RL. 2000. Functional analysis of spermatogonial stem cells in Steel and cryptorchid infertile mouse models. Dev Biol 220(2):401-11. [PubMed: 10753526]  [MGI Ref ID J:61712]

Silver DL; Hou L; Somerville R; Young ME; Apte SS; Pavan WJ. 2008. The secreted metalloprotease AMAMTS20 is required for melanoblast survival PLoS Genet 4(2):e1000003. [PubMed: 18454205]  [MGI Ref ID J:133403]

Steel KP; Davidson DR; Jackson IJ. 1992. TRP-2/DT, a new early melanoblast marker, shows that steel growth factor (c-kit ligand) is a survival factor. Development 115(4):1111-9. [PubMed: 1280558]  [MGI Ref ID J:31646]

Stokol T; O'Donnell P; Xiao L; Knight S; Stavrakis G; Botto M; von Andrian UH; Mayadas TN. 2004. C1q governs deposition of circulating immune complexes and leukocyte Fcgamma receptors mediate subsequent neutrophil recruitment. J Exp Med 200(7):835-46. [PubMed: 15466618]  [MGI Ref ID J:93949]

Sundberg JP; Kenty GA; Beamer WG; Adkison DL. 1992. Forestomach papillomas in flaky skin and steel-Dickie mutant mice. J Vet Diagn Invest 4(3):312-7. [PubMed: 1325193]  [MGI Ref ID J:2777]

Tadokoro Y; Yomogida K; Ohta H; Tohda A; Nishimune Y. 2002. Homeostatic regulation of germinal stem cell proliferation by the GDNF/FSH pathway. Mech Dev 113(1):29-39. [PubMed: 11900972]  [MGI Ref ID J:184664]

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Tokuda M; Kadokawa Y; Kurahashi H; Marunouchi T. 2007. CDH1 is a specific marker for undifferentiated spermatogonia in mouse testes. Biol Reprod 76(1):130-41. [PubMed: 17035642]  [MGI Ref ID J:117360]

Turner RT; Wong CP; Iwaniec UT. 2011. Effect of reduced c-Kit signaling on bone marrow adiposity. Anat Rec (Hoboken) 294(7):1126-34. [PubMed: 21634019]  [MGI Ref ID J:175574]

Turnquist HR; Zhao Z; Rosborough BR; Liu Q; Castellaneta A; Isse K; Wang Z; Lang M; Stolz DB; Zheng XX; Demetris AJ; Liew FY; Wood KJ; Thomson AW. 2011. IL-33 expands suppressive CD11b+ Gr-1 int and regulatory T cells, including ST2L+ Foxp3+ cells, and mediates regulatory T cell-dependent promotion of cardiac allograft survival. J Immunol 187(9):4598-610. [PubMed: 21949025]  [MGI Ref ID J:179437]

Wang CH; Anderson N; Li SH; Szmitko PE; Cherng WJ; Fedak PW; Fazel S; Li RK; Yau TM; Weisel RD; Stanford WL; Verma S. 2006. Stem cell factor deficiency is vasculoprotective: unraveling a new therapeutic potential of imatinib mesylate. Circ Res 99(6):617-25. [PubMed: 16931795]  [MGI Ref ID J:125065]

Watanabe M; Satoh T; Yamamoto Y; Kanai Y; Karasuyama H; Yokozeki H. 2008. Overproduction of IgE induces macrophage-derived chemokine (CCL22) secretion from basophils. J Immunol 181(8):5653-9. [PubMed: 18832724]  [MGI Ref ID J:140755]

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Wershil BK; Castagliuolo I; Pothoulakis C. 1998. Direct evidence of mast cell involvement in Clostridium difficile toxin A-induced enteritis in mice. Gastroenterology 114(5):956-64. [PubMed: 9558284]  [MGI Ref ID J:107762]

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Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3300.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $4290.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
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Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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