Strain Name: |
WB.D2-KitlSl-d/J |
|---|---|
Stock Number: |
000161 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
MgfSl-di (Changed: 15-DEC-04
) |
| Genes & Alleles | Kitl; KitlSl-d; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Species laboratory mouse Background Strain WB/Re Donor Strain DBA/2J Generation N42 Strain Description
The multiple steel mutations (KitlSl) behave in a semidominant fashion and cause deficiencies in pigment cells, germ cells, and blood cells paralleling those caused by the Kit locus mutations (dominant spotting alleles). Most of the alleles at steel locus cause severe anemia in utero and death by 15 to 16 days of gestation in homozygous mutant mice. However, steel-Dickie homozygotes (KitlSl-d/KitlSl-d) and compounds of steel and steel Dickie (KitlSl/KitlSl-d) are viable, black-eyed white, are usually sterile in one or both sexes, and have severe macrocytic anemia. Heterozygous steel mice have a diluted coat color with a small amount of white spotting, are viable and fertile, and may have a slight macrocytic anemia. Primordial germ cells are absent in the nonviable steel homozygotes and severely reduced in steel Dickie and steel heterozygotes. Mast cells are virtually absent in skin and other tissues of steel mutant mice. Tumors tend to develop in germ-cell-deficient ovaries with advancing age.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that thephenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | KitlSl-d | ||
|---|---|---|---|
| Allele Name | steel Dickie | ||
| Common Name(s) | KL; MGF; MgfSl-d; Sld; Sld; | ||
| Strain of Origin | DBA/2J | ||
| Gene Symbol and Name | Kitl, kit ligand | ||
| Chromosome | 10 | ||
| Gene Common Name(s) | Clo; Con; DKFZp686F2250; Gb; KL-1; Kl1; Kl2; MGF; Mgf; SCF; SF; SHEP7; SLF; Sl; Steel; Steel factor; cloud gray; contrasted; grizzle-belly; mast cell growth factor; steel; stem cell factor; | ||
| General Note |
Genbank ID for this allele: M64262 | ||
| Molecular Note | A 4kb deletion in genomic DNA results in the absence of 241bp of wild type cDNA and the addition of 67bp of novel sequence, a 174bp net loss. The region that is deleted begins 5 amino acids N-terminal to the transmembrane domain and results in termination of the open reading frame after an additional 3 amino acids. The resulting protein is a soluble truncated one, lacking both transmembrane and cytoplasmic domains. Northern analysis indicates that mRNA is transcribed at nearly wild-type levels in adult tiss [J:10750] [J:20286] [J:40339] | ||
Control Information
| Allele | Control | |
|---|---|---|
| KitlSl-d | Wild-type from the colony | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying KitlSl-d allele
000160 B6.D2-KitlSl-d/J 100401 WCB6F1/J KitlSl KitlSl-d View Strains carrying KitlSl-d (2 strains)
000090 129S1/Sv-Oca2+ Tyr+ KitlSl-J/J 002993 B6.Cg-KitlSl-18H/EiJ 000124 B6.Cg-KitlSl Krt71Ca/J 000291 C3FeLe.Cg-a/a Hm KitlSl Krt71Ca-J/J 001380 C3Sn.Cg-KitlSl-con/J 003252 C57BL/6J-KitlSl-20J/J 006839 C57BL/6J-KitlSl-22J/J 000979 STOCK KitlSl-16J/J 000693 WC/ReJ KitlSl/J 100401 WCB6F1/J KitlSl KitlSl-d
Additional Web Information
Congenic Nomenclature
JAX Notes, February 2001; 481. Mgf Gene Name Changes to Kitl.
Research Applications
This mouse can be used to support research in many areas including:KitlSl-d related
Cancer Research
Growth Factors/Receptors/Cytokines
Increased Tumor Incidence (Gonadal Tumors: ovarian and testicular)
Dermatology Research
Color and White Spotting Defects
Developmental Biology Research
Neural Tube Defects
Endocrine Deficiency Research
Bone/Bone Marrow Defects
Gonad Defects
Hypothalamus/Pituitary Defects
Skin Defects
Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Immunodeficiency (Mast Cell Deficiency)
Neurobiology Research
Vestibular and Hearing Defects
Reproductive Biology Research
Developmental Defects Affecting Gonads (germ cell deficient)
Fertility Defects
Gonadal Tumors (ovarian and testicular)
Research Tools
Immunology and Inflammation Research (Mast Cell Deficiency)
Sensorineural Research
Vestibular and Hearing Defects
References
Additional ReferencesPrice and Supply Information
| Strain Name: | WB.D2-KitlSl-d/J |
| Stock Number: | 000161 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
*NO Shipping Destination selected!
Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to the Supply Notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form