Description

Strain Information

Former Names C3H/HeJ-md/+    (Changed: 15-DEC-04 ) Type Coisogenic; Mutant Strain; Spontaneous Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse

Development
Mahoganoid (Mgrn^md) arose spontaneously in strain C3H/HeJ at the Jackson Laboratory in 1957. Homozygotes were sibling mated to F5 and then a homozygous female was crossed to an unpedigreed C3H/HeJ male. The stock was then sibling mated using forced heterozygosis. It was cryopreserved in 1998 at F158 by mating homozygous females to heterozygous males.

Control Information

	Control
	Heterozygote from the colony
Considerations for Choosing Controls

Related Strains

Strains carrying   Mgrn1^md allele

000001	B6.C3 A/a Mgrn1md/J
000309	B6.C3-Mgrn1md/J
001277	STOCK Mgrn1md Igl-1b Igl1-rlo/J

View Strains carrying   Mgrn1^md     (3 strains)

Strains carrying other alleles of Mgrn1

000809

C3H/HeJ-Mgrn1md-2J/J

View Strains carrying other alleles of Mgrn1     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Mgrn1^md/Mgrn1^md

        C3H/HeJ-Mgrn1^md/J

• pigmentation phenotype
• darkened coat color (MGI Ref ID J:81466)
  • reduced amount of yellow pigment causing a dark brown appearance
• skin/coat/nails phenotype
• darkened coat color (MGI Ref ID J:81466)
  • reduced amount of yellow pigment causing a dark brown appearance

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Mgrn1^md related

Dermatology Research
Color and White Spotting Defects
Skin and Hair Texture Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Mgrn1md
Allele Name		mahoganoid
Allele Type		Spontaneous
Common Name(s)		md;
Strain of Origin		C3H/HeJ
Gene Symbol and Name		Mgrn1, mahogunin, ring finger 1
Chromosome		16
Gene Common Name(s)		2610042J20Rik; KIAA0544; N28182; RGD1311862; RIKEN cDNA 2610042J20 gene; RNF156; expressed sequence N28182; mKIAA0544; mahoganoid; md; nc; non-agouti curly; non-agouti-curly;
General Note		The original md mutation arose in the C3H/HeJ strain. This gene is similar to mahogany (Atrnmg) in its effects on coat color, but causes less darkening of the back, ears, and tail of agouti mice (J:13379)(J:13791).
Molecular Note		The mutation in md has been attributed to a 5 kb retroviral insertion located in the intronic region 5' of exon 12. Northern analysis and RT-PCR indicate that the expression of transcript is greatly reduced in these mice. [MGI Ref ID J:80182]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Additional References

Mgrn1^md related

Bagher P; Jiao J; Owen Smith C; Cota CD; Gunn TM. 2006. Characterization of Mahogunin Ring Finger-1 expression in mice. Pigment Cell Res 19(6):635-43. [PubMed: 17083490]  [MGI Ref ID J:119125]

He L; Lu XY; Jolly AF; Eldridge AG; Watson SJ; Jackson PK; Barsh GS; Gunn TM. 2003. Spongiform degeneration in mahoganoid mutant mice. Science 299(5607):710-2. [PubMed: 12560552]  [MGI Ref ID J:81466]

Lane PW. 1960. mahoganoid, md Mouse News Lett 22:35.  [MGI Ref ID J:13379]

Miller KA; Gunn TM; Carrasquillo MM; Lamoreux ML; Galbraith DB ; Barsh GS. 1997. Genetic studies of the mouse mutations mahogany and mahoganoid. Genetics 146(4):1407-15. [PubMed: 9258683]  [MGI Ref ID J:41964]

Phan LK; Chung WK; Leibel RL. 2006. The mahoganoid mutation (Mgrn1md) improves insulin sensitivity in mice with mutations in the melanocortin signaling pathway independently of effects on adiposity. Am J Physiol Endocrinol Metab 291(3):E611-20. [PubMed: 16638826]  [MGI Ref ID J:112364]

Phan LK; Lin F; LeDuc CA; Chung WK; Leibel RL. 2002. The mouse mahoganoid coat color mutation disrupts a novel C3HC4 RING domain protein. J Clin Invest 110(10):1449-59. [PubMed: 12438443]  [MGI Ref ID J:80182]

Phillips RJ; Fisher G. 1979. Non-agouti curly: change of symbol. Mouse News Lett 60:46-47.  [MGI Ref ID J:13791]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Heterozygote from the colony
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

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