Strain Name:

C3FeLe.B6 a/a-Ptpn6me/J

Stock Number:

000225

Availability:

Repository-Cryopreserved

Description

Strain Information

Former Names C3FeLe.B6 a/a-Hcphme/J    (Changed: 16-JUN-05 )
C3FeLe.B6 a/a-Hcphme/+    (Changed: 15-DEC-04 )
Type Congenic; Mutant Strain;
Specieslaboratory mouse
Background Strain C3HeB/FeJLe
Donor Strain C57BL/6J
H2 Haplotypek

Appearance
black
Related Genotype: a/a

Description
Mice homozygous for the motheaten spontaneous mutation (Ptpn6me) develop severe autoimmune disease. Characteristics include by granulocytic skin lesions, pneumonitis, impaired humoral and cell-mediated immune responses, decreased responses to T cell and B cell mitogens and deficient cytotoxic T cell and NK cell activity. B cells are LY-1+. Homozygous mutant mice also exhibit hyperimmunoglobulinemia, and express multiple autoantibodies. Macrophages show increased proliferative capacity. In addition to defects in the immune system, motheaten mice show classic symptoms of osteoporosis due to an increased number and activity of osteoclasts in the bone marrow. The lifespan of homozygous motheaten mice is approximately 3 weeks with death attributed to an autoimmune pneumonitis.

Control Information

  Control
   Untyped from the colony
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Ptpn6me allele
000810   C57BL/6J-Ptpn6me/J
View Strains carrying   Ptpn6me     (1 strain)

Strains carrying   a allele
003879   B10;TFLe-a/a T tf/+ tf/J
001538   B6 x B6C3Sn a/A-T(1;9)27H/J
000916   B6 x B6C3Sn a/A-T(5;12)31H/J
000602   B6 x B6C3Sn a/A-T(8;16)17H/J
000618   B6 x FSB/GnEi a/a Ctslfs/J
000577   B6 x STOCK a Oca2p Hps5ru2 Ednrbs/J
000601   B6 x STOCK a/a T(7;18)50H/J
000592   B6 x STOCK T(2;4)13H a/J
000001   B6.C3 A/a Mgrn1md/J
000785   B6;D2-a Es1e/J
000604   B6C3 a/A-T(10;13)199H +/+ Lystbg-J/J or Lystbg-2J/J
002807   B6C3Fe a/a-Meox2fla/J
000224   B6C3Fe a/a-Scyl1mdf/J
001037   B6C3Fe a/a-Agtpbp1pcd/J
000221   B6C3Fe a/a-Alx4lst-J/J
002062   B6C3Fe a/a-Atp7aMo-8J/J
001756   B6C3Fe a/a-Cacng2stg/J
001815   B6C3Fe a/a-Col1a2oim/J
000231   B6C3Fe a/a-Csf1op/J
000209   B6C3Fe a/a-Dh/J
000211   B6C3Fe a/a-Dstdt-J/J
000210   B6C3Fe a/a-Edardl-J/J
000207   B6C3Fe a/a-Edaraddcr/J
000182   B6C3Fe a/a-Eef1a2wst/J
001278   B6C3Fe a/a-Glra1spd/J
000241   B6C3Fe a/a-Glrbspa/J
002875   B6C3Fe a/a-Hoxd13spdh/J
000304   B6C3Fe a/a-Krt71Ca Scn8amed-J/J
000226   B6C3Fe a/a-Largemyd/J
000636   B6C3Fe a/a-Lmx1adr-J/J
001280   B6C3Fe a/a-Lse/J
001573   B6C3Fe a/a-MitfMi/J
001035   B6C3Fe a/a-Napahyh/J
000181   B6C3Fe a/a-Otogtwt/J
000278   B6C3Fe a/a-Papss2bm Hps1ep Hps6ru/J
000205   B6C3Fe a/a-Papss2bm/J
002078   B6C3Fe a/a-Pcdh15av-2J/J
000246   B6C3Fe a/a-Pitpnavb/J
001430   B6C3Fe a/a-Ptch1mes/J
000506   B6C3Fe a/a-Qkqk/J
000235   B6C3Fe a/a-Relnrl/J
000237   B6C3Fe a/a-Rorasg/J
000290   B6C3Fe a/a-Sox10Dom/J
000230   B6C3Fe a/a-Tcirg1oc/J
003612   B6C3Fe a/a-Trak1hyrt/J
001512   B6C3Fe a/a-Ttnmdm/J
001607   B6C3Fe a/a-Unc5crcm/J
000005   B6C3Fe a/a-Wc/J
000243   B6C3Fe a/a-Wnt1sw/J
000248   B6C3Fe a/a-Xpl/J
001750   B6C3Fe a/a-XsJ/J
000624   B6C3Fe a/a-anx/J
003020   B6C3Fe a/a-dep/J
002018   B6C3Fe a/a-din/J
002339   B6C3Fe a/a-nma/J
000240   B6C3Fe a/a-soc/J
000063   B6C3Fe a/a-sy/J
001055   B6C3Fe a/a-tip/J
000245   B6C3Fe a/a-tn/J
000296   B6C3Fe-a/a Hoxa13Hd Mcoln3Va-J/J
000019   B6C3Fe-a/a-Itpr1opt/J
001022   B6C3FeF1/J a/a
000971   B6EiC3 a/A-Och/J
000551   B6EiC3 a/A-Tbx15de-H/J
006450   B6EiC3 a/A-Vss/J
000557   B6EiC3-+ a/LnpUl A/J
000503   B6EiC3Sn a/A-Gy/J
001811   B6EiC3Sn a/A-Otcspf-ash/J
002343   B6EiC3Sn a/A-Otcspf/J
000391   B6EiC3Sn a/A-Pax6Sey-Dey/J
001924   B6EiC3Sn a/A-Ts(1716)65Dn
001923   B6EiC3Sn a/A-Ts(417)2Lws Tim/J
000198   C3FeLe.B6-a/J
000291   C3FeLe.Cg-a/a Hm KitlSl Krt71Ca-J/J
001886   C3HeB/FeJLe a/a-gnd/J
000584   C57BL/6J-+ T(1;2)5Ca/a +/J
000284   CWD/LeJ
000670   DBA/1J
000671   DBA/2J
001057   HPT/LeJ
000260   JGBF/LeJ
000265   MY/HuLeJ
000308   SSL/LeJ
000994   STOCK a Myo5ad Mregdsu/J
000064   STOCK a Tyrp1b Sisi/J
002238   STOCK a Tyrp1b shmy/J
001433   STOCK a skt/J
000579   STOCK a tp/J
000319   STOCK a us/J
002648   STOCK a/a Cln6nclf/J
000317   STOCK a/a Egfrwa2/J
000302   STOCK a/a MitfMi-wh +/+ Itpr1opt/J
000286   STOCK a/a Myo5ad fd/+ +/J
000206   STOCK a/a Tyrc-h/J
001432   STOCK a/a Tyrp1b sks/Tyrp1b +/J
000281   STOCK a/a ma ft/ma ft/J
000312   STOCK stb + a/+ Fignfi a/J
000596   STOCK T(2;11)30H/+ x AEJ-a Gdf5bp-H/J or A/J-a Gdf5bp-J/J
000970   STOCK T(2;16)28H A/T(2;16)28H a/J
000590   STOCK T(2;4)1Sn a/J
000594   STOCK T(2;8)26H a/T(2;8)26H a Tyrp1+/Tyrp1b/J
000623   TR/DiEiJ
View Strains carrying   a     (102 strains)

Strains carrying other alleles of Ptpn6
000811   C57BL/6J-Ptpn6me-v/J
View Strains carrying other alleles of Ptpn6     (1 strain)

Strains carrying other alleles of a
003301   (C57BL/6J x C3H-Eya1bor)F1/J
000251   AEJ.Cg-ae +/a Gdf5bp-H/J
000202   AEJ/Gn-bd/J
000199   AEJ/GnLeJ
000427   B10.CE-H13b Aw/(30NX)SnJ
000420   B10.LP-H13b Aw/Sn
000477   B10.PA-Pldnpa H3e at/SnJ
000419   B10.UW-H3b we Pax1un at/SnJ
000593   B6 x B6CBCa Aw-J/A-Grid2Lc T(2;6)7Ca MitfMi-wh/J
000502   B6 x B6CBCa Aw-J/A-Myo5aflr Gnb5flr/J
000599   B6 x B6CBCa Aw-J/A-T(5;13)264Ca KitW-v/J
002083   B6 x B6EiC3 a/A-T(7;16)235Dn/J
000507   B6 x B6EiC3 a/A-Otcspf/J
002016   B6(Cg)-Aw-J EdaTa-6J Chr YB6-Sxr/EiJ
000552   B6-Aw-J-EdaTa-6J.Cg-Sxr
001730   B6-Aw-J-EdaTa-6J.Cg-Sxrb Hya-/J
000841   B6-Aw-J.CBy-EdaTa-By/J
001809   B6-Aw-J.Cg-EdaTa-6J +/+ ArTfm/J
000600   B6-Gpi1b x B6CBCa Aw-J/A-T(7;15)9H Gpi1a/J
000769   B6.C/(HZ18)By-at-44J/J
000203   B6.C3-Aiy/a/J
001572   B6.C3-am-J/J
000017   B6.C3Fe-Avy/J
000628   B6.CE-A Amy1b Amy2b/J
005505   B6.Cg-Ay Slc7a11sut/LmLlp
000021   B6.Cg-Ay/J
100409   B6129PF1/J-Aw-J/Aw
004200   B6;CBACa Aw-J/A-Npr2cn-2J/J
000505   B6C3 Aw-J/A-Mutedmu/J
000604   B6C3 a/A-T(10;13)199H +/+ Lystbg-J/J or Lystbg-2J/J
000065   B6C3Fe a/a-we Pax1un at/J
000314   B6CBACa Aw-J/A-EdaTa/J-XO
000501   B6CBACa Aw-J/A-Aifm1Hq/J
001046   B6CBACa Aw-J/A-Grid2Lc/J
000500   B6CBACa Aw-J/A-Gs/J
002703   B6CBACa Aw-J/A-Hydinhy3/J
000247   B6CBACa Aw-J/A-Kcnj6wv/J
000287   B6CBACa Aw-J/A-Plp1jp EdaTa/J
000515   B6CBACa Aw-J/A-SfnEr/J
000242   B6CBACa Aw-J/A-spc/J
000288   B6CBACa Aw-J/A-we a Mafbkr/J
001201   B6CBACaF1/J-Aw-J/A
001752   B6CBCa Aw-J/A-T(7;15)9H/J
006450   B6EiC3 a/A-Vss/J
000557   B6EiC3-+ a/LnpUl A/J
000504   B6EiC3Sn a/A-Cacnb4lh/J
000553   B6EiC3Sn a/A-Egfrwa2 Wnt3avt/J
001811   B6EiC3Sn a/A-Otcspf-ash/J
002343   B6EiC3Sn a/A-Otcspf/J
001923   B6EiC3Sn a/A-Ts(417)2Lws Tim/J
000200   C3FeB6 A/Aw-J-Ankank/J
000638   C3FeB6 A/Aw-J-Spnb4qv-J/J
001203   C3FeB6F1/J A/Aw-J
001272   C3H/HeSnJ-Ahvy/J
000099   C3HeB/FeJ-Avy/J
000338   C57BL/6J Aw-J-EdaTa-6J/J
000258   C57BL/6J-Ai/a/J
000774   C57BL/6J-Asy/a/J
000569   C57BL/6J-Aw-J-EdaTa +/+ ArTfm/J
000051   C57BL/6J-Aw-J/J
000055   C57BL/6J-at-33J/J
000070   C57BL/6J-atd/J
002468   KK.Cg-Ay/J
000262   LS/LeJ
000283   LT.CAST-A/J
001759   STOCK A Tyrc Sha/J
001427   STOCK Aw us/J
View Strains carrying other alleles of a     (67 strains)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

Mammalian Phenotype Terms assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Ptpn6me/Ptpn6me

        C57BL/6J-Ptpn6me/J
  • skin/coat/nails phenotype
  • abnormal coat appearance (MGI Ref ID J:5579)
    • patchy absence of hair as the coat appears gives mice a motheaten appearance
    • patchy hair (MGI Ref ID J:5579)
  • abnormal skin pigmentation (MGI Ref ID J:5579)
    • recognized at 3 to 4 days of age by patchy absence of skin pigment
  • skin lesions (MGI Ref ID J:5579)
    • rapidly progressing lesions develop on the feet by 3 weeks of age
    • as early as 2 days after birth abscesses appear on the skin, rupture and begin to heal in 24 hrs
  • pigmentation phenotype
  • abnormal skin pigmentation (MGI Ref ID J:5579)
    • recognized at 3 to 4 days of age by patchy absence of skin pigment
  • immune system phenotype
  • abnormal Peyer's patch morphology (MGI Ref ID J:5579)
    • rarely any differentiation of lymphoid tissue into nodules is found
    • lymphocytes are larger and sparsely distributed
  • abnormal humoral immune response (MGI Ref ID J:5579)
    • mice are deficient in capacity for immune response
    • increased immunoglobulin level (MGI Ref ID J:5579)
      • increased IgA level (MGI Ref ID J:5579)
        • serum levels are elevated
      • increased IgG level (MGI Ref ID J:5579)
        • serum levels are elevated
      • increased IgM level (MGI Ref ID J:5579)
        • serum levels are markedly elevated above normal
  • abnormal immune system cell morphology (MGI Ref ID J:5579)
    • mice have a population of unusual binucleate lymphocytes
    • abnormal granulocyte morphology (MGI Ref ID J:5579)
      • differential counts of blood from 1 to 3 day old mice showed an immature granuloctye population
    • abnormal leukocyte cell number (MGI Ref ID J:5579)
      • increased leukocyte cell number (MGI Ref ID J:5579)
        • increased granulocyte number (MGI Ref ID J:5579)
        • increased monocyte cell number (MGI Ref ID J:5579)
  • abnormal thymus morphology (MGI Ref ID J:5579)
    • small thymus (MGI Ref ID J:5579)
      • although smaller than normal, the thymus is histologically normal until 25 days of age
      • severe involution and necrosis is found in sick mice, with severity correlating with degree of illness
  • enlarged spleen (MGI Ref ID J:5579)
  • life span-post-weaning/aging
  • premature death (MGI Ref ID J:5579)
    • mortality is high from birth onward with none surviving longer than 8 weeks
  • hematopoietic system phenotype
  • abnormal granulocyte morphology (MGI Ref ID J:5579)
    • differential counts of blood from 1 to 3 day old mice showed an immature granuloctye population
  • abnormal leukocyte cell number (MGI Ref ID J:5579)
    • increased leukocyte cell number (MGI Ref ID J:5579)
      • increased granulocyte number (MGI Ref ID J:5579)
      • increased monocyte cell number (MGI Ref ID J:5579)
  • abnormal thymus morphology (MGI Ref ID J:5579)
    • small thymus (MGI Ref ID J:5579)
      • although smaller than normal, the thymus is histologically normal until 25 days of age
      • severe involution and necrosis is found in sick mice, with severity correlating with degree of illness
  • enlarged spleen (MGI Ref ID J:5579)
  • respiratory system phenotype
  • abnormal lung morphology (MGI Ref ID J:5579)
    • mice develop lesions in the lung by 3 days of age
  • cellular phenotype
  • decreased apoptosis (MGI Ref ID J:66843)
    • only 21% of spleen cells are apoptotic 6 hours after exposure to 5 Gy gamma irradiation, whereas 40% of wildtype splenocytes are apoptotic after treatment.
    • B and T cells show the greatest resistance to apoptosis in the splenocyte population, but all splenic cell types including macrophages and granulocytes have greater resistance to apoptosis than wildtype cells
  • decreased cellular sensitivity to gamma-irradiation (MGI Ref ID J:66843)
    • the LD50 for homozygous spleen cells is 24.5 Gy gamma radiation versus 6.5 Gy for wildtype splenocytes

Research Applications

This mouse can be used to support research in many areas including:

Ptpn6me related

Apoptosis Research

Dermatology Research
Skin and Hair Texture Defects

Endocrine Deficiency Research
Bone/Bone Marrow Defects
Thyroid Defects

Immunology and Inflammation Research
Autoimmunity
Immunodeficiency
Inflammation

Internal/Organ Research
Lymphoid Tissue Defects
Spleen Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol Ptpn6me
Allele Name motheaten
Common Name(s) me;
Strain of OriginC57BL/6J
Gene Symbol and Name Ptpn6, protein tyrosine phosphatase, non-receptor type 6
Chromosome 6
Gene Common Name(s) HCP; HCPH; HPTP1C; Hcph; MGC124580; PTP-1C; Ptp1C; Ptph6; SH-PTP1; SHP-1; SHP-1L; SHP1; hemopoietic cell phosphatase; me; motheaten;
General Note Homozygotes are characterized by early onset autoimmunity and severe immunodeficiency (J:5579). Mice develop an unusual pneumonia which progresses to accumulation of crystal-containing macrophages in the alveoli (J:5999). Spleen macrophages of homozygotes have an accelerated rate of proliferation which may contribute to the pulmonary disease (J:7274). The number of surface-Ig-bearing lymphocytes (B-cells) is greatly reduced, and the B-cells present are of immature rather than adult type (J:6065). The number of T-cells is normal, but several T-cell functions are defective. Natural killer cell activity is virtually absent (J:6485).

Genbank ID for this mutation: S63764

Molecular Note A single nucleotide (C) deletion at position 228 creates a cryptic splice site. This results in the deletion of a 101bp segment in the encoded transcript, and a frameshift in the encoded protein. [MGI Ref ID J:11892] [MGI Ref ID J:60297]
 
Allele Symbol a
Allele Name nonagouti

Genotyping

Genotyping Information

Genotyping Protocols

Ptpn6me, REST, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Additional References

Clark EA; Shultz LD; Pollack SB. 1981. Mutations in mice that influence natural killer (NK) cell activity. Immunogenetics 12(5-6):601-13. [PubMed: 6971254]  [MGI Ref ID J:6485]

Davidson WF; Morse HC 3d; Sharrow SO; Chused TM. 1979. Phenotypic and functional effects of the motheaten gene on murine B and T lymphocytes. J Immunol 122(3):884-91. [PubMed: 221571]  [MGI Ref ID J:6135]

Green MC; Shultz LD. 1975. Motheaten, an immunodeficient mutant of the mouse. I. Genetics and pathology. J Hered 66(5):250-8. [PubMed: 1184950]  [MGI Ref ID J:5579]

Kozlowski M; Mlinaric-Rascan I; Feng GS; Shen R; Pawson T; Siminovitch KA. 1993. Expression and catalytic activity of the tyrosine phosphatase PTP1C is severely impaired in motheaten and viable motheaten mice. J Exp Med 178(6):2157-63. [PubMed: 8245788]  [MGI Ref ID J:15725]

McCoy KL; Clagett J; Rosse C. 1985. Effects of the motheaten gene on murine B-cell production. Exp Hematol 13(6):554-9. [PubMed: 3873348]  [MGI Ref ID J:7856]

McCoy KL; Nielson K; Clagett J. 1984. Spontaneous production of colony-stimulating activity by splenic Mac-1 antigen-positive cells from autoimmune motheaten mice. J Immunol 132(1):272-6. [PubMed: 6361123]  [MGI Ref ID J:7274]

Mlinaric-Rascan I; Asa SL; Siminovitch KA. 1994. Increased expression of the stefin A cysteine proteinase inhibitor occurs in the myelomonocytic cell-infiltrated tissues of autoimmune motheaten mice. Am J Pathol 145(4):902-12. [PubMed: 7943179]  [MGI Ref ID J:20878]

Shultz LD; Green MC. 1976. Motheaten, an immunodeficient mutant of the mouse. II. Depressed immune competence and elevated serum immunoglobulins. J Immunol 116(4):936-43. [PubMed: 56406]  [MGI Ref ID J:5624]

Shultz LD; Schweitzer PA; Rajan TV; Yi T; Ihle JN; Matthews RJ; Thomas ML; Beier DR. 1993. Mutations at the murine motheaten locus are within the hematopoietic cell protein-tyrosine phosphatase (Hcph) gene. Cell 73(7):1445-54. [PubMed: 8324828]  [MGI Ref ID J:14935]

Sidman CL; Shultz LD; Unanue ER. 1978. The mouse mutant motheaten. II. Functional studies of the immune system. J Immunol 121(6):2399-404. [PubMed: 363947]  [MGI Ref ID J:6065]

Tsui HW; Siminovitch KA; de Souza L; Tsui FW. 1993. Motheaten and viable motheaten mice have mutations in the haematopoietic cell phosphatase gene. Nat Genet 4(2):124-9. [PubMed: 8348149]  [MGI Ref ID J:11892]

Wishcamper CA; Coffin JD; Lurie DI. 2001. Lack of the protein tyrosine phosphatase SHP-1 results in decreased numbers of glia within the motheaten (me/me) mouse brain. J Comp Neurol 441(2):118-33. [PubMed: 11745639]  [MGI Ref ID J:72655]

Yi T; Gilbert DJ; Jenkins NA; Copeland NG; Ihle JN. 1992. Assignment of a novel protein tyrosine phosphatase gene (Hcph) to mouse chromosome 6. Genomics 14(3):793-5. [PubMed: 1427910]  [MGI Ref ID J:3085]

Zhao J; Lurie DI. 2004. Cochlear ablation in mice lacking SHP-1 results in an extended period of cell death of anteroventral cochlear nucleus neurons. Hear Res 189(1-2):63-75. [PubMed: 14987753]  [MGI Ref ID J:88807]

Ptpn6me related

Berg KL; Siminovitch KA; Stanley ER. 1999. SHP-1 regulation of p62(DOK) tyrosine phosphorylation in macrophages. J Biol Chem 274(50):35855-65. [PubMed: 10585470]  [MGI Ref ID J:58900]

Bignon JS; Siminovitch KA. 1994. Identification of PTP1C mutation as the genetic defect in motheaten and viable motheaten mice: a step toward defining the roles of protein tyrosine phosphatases in the regulation of hemopoietic cell differentiation and function. Clin Immunol Immunopathol 73(2):168-79. [PubMed: 7923924]  [MGI Ref ID J:21151]

Carter JD; Calabrese GM; Naganuma M; Lorenz U. 2005. Deficiency of the Src homology region 2 domain-containing phosphatase 1 (SHP-1) causes enrichment of CD4+CD25+ regulatory T cells. J Immunol 174(11):6627-38. [PubMed: 15905501]  [MGI Ref ID J:99043]

Carter JD; Neel BG; Lorenz U. 1999. The tyrosine phosphatase SHP-1 influences thymocyte selection by setting TCR signaling thresholds. Int Immunol 11(12):1999-2014. [PubMed: 10590266]  [MGI Ref ID J:59134]

Clark EA; Shultz LD; Pollack SB. 1981. Mutations in mice that influence natural killer (NK) cell activity. Immunogenetics 12(5-6):601-13. [PubMed: 6971254]  [MGI Ref ID J:6485]

Davidson D; Bakinowski M; Thomas ML; Horejsi V; Veillette A. 2003. Phosphorylation-dependent regulation of T-cell activation by PAG/Cbp, a lipid raft-associated transmembrane adaptor. Mol Cell Biol 23(6):2017-28. [PubMed: 12612075]  [MGI Ref ID J:113969]

Green MC; Shultz LD. 1975. Motheaten, an immunodeficient mutant of the mouse. I. Genetics and pathology. J Hered 66(5):250-8. [PubMed: 1184950]  [MGI Ref ID J:5579]

Hsu HC; Shultz LD; Su X; Shi J; Yang PA; Relyea MJ; Zhang HG; Mountz JD. 2001. Mutation of the hematopoietic cell phosphatase (Hcph) gene is associated with resistance to gamma-irradiation-induced apoptosis in Src homology protein tyrosine phosphatase (SHP)-1-deficient 'motheaten' mutant mice. J Immunol 166(2):772-80. [PubMed: 11145649]  [MGI Ref ID J:66843]

Jiao H; Yang W; Berrada K; Tabrizi M; Shultz L; Yi T. 1997. Macrophages from motheaten and viable motheaten mutant mice show increased proliferative responses to GM-CSF: detection of potential HCP substrates in GM-CSF signal transduction. Exp Hematol 25(7):592-600. [PubMed: 9216734]  [MGI Ref ID J:41396]

Johnson KG; LeRoy FG; Borysiewicz LK; Matthews RJ. 1999. TCR signaling thresholds regulating T cell development and activation are dependent upon SHP-1. J Immunol 162(7):3802-13. [PubMed: 10201897]  [MGI Ref ID J:53576]

Kamata T; Yamashita M; Kimura M; Murata K; Inami M; Shimizu C; Sugaya K; Wang CR; Taniguchi M; Nakayama T. 2003. src homology 2 domain-containing tyrosine phosphatase SHP-1 controls the development of allergic airway inflammation. J Clin Invest 111(1):109-19. [PubMed: 12511594]  [MGI Ref ID J:81122]

Kon-Kozlowski M; Pani G; Pawson T; Siminovitch KA. 1996. The tyrosine phosphatase PTP1C associates with Vav, Grb2, and mSos1 in hematopoietic cells. J Biol Chem 271(7):3856-62. [PubMed: 8632004]  [MGI Ref ID J:31423]

Kozlowski M; Mlinaric-Rascan I; Feng GS; Shen R; Pawson T; Siminovitch KA. 1993. Expression and catalytic activity of the tyrosine phosphatase PTP1C is severely impaired in motheaten and viable motheaten mice. J Exp Med 178(6):2157-63. [PubMed: 8245788]  [MGI Ref ID J:15725]

Kruger J; Butler JR; Cherapanov V; Dong Q; Ginzberg H; Govindarajan A; Grinstein S; Siminovitch KA; Downey GP. 2000. Deficiency of Src homology 2-containing phosphatase 1 results in abnormalities in murine neutrophil function: studies in motheaten mice. J Immunol 165(10):5847-59. [PubMed: 11067945]  [MGI Ref ID J:118386]

Kuntz L; Jachez B; Roman D; Loor F. 1993. Modulation of adoptively transferred viable motheaten pathology in sublethally irradiated normal recipient mice by normal hematopoietic cells. Cell Immunol 146(1):215-21. [PubMed: 8093859]  [MGI Ref ID J:3800]

Lorenz U; Bergemann AD; Steinberg HN; Flanagan JG; Li X; Galli SJ; Neel BG. 1996. Genetic analysis reveals cell type-specific regulation of receptor tyrosine kinase c-Kit by the protein tyrosine phosphatase SHP1. J Exp Med 184(3):1111-26. [PubMed: 9064328]  [MGI Ref ID J:35276]

Lutzner MA; Hansen CT. 1976. Motheater: an immunodeficient mouse with markedly less ability to survive that the nude mouse in a germfree environment. J Immunol 116(5):1496-7. [PubMed: 1270804]  [MGI Ref ID J:5648]

Lyons BL; Smith RS; Hurd RE; Hawes NL; Burzenski LM; Nusinowitz S; Hasham MG; Chang B; Shultz LD. 2006. Deficiency of SHP-1 protein-tyrosine phosphatase in 'viable motheaten' mice results in retinal degeneration. Invest Ophthalmol Vis Sci 47(3):1201-9. [PubMed: 16505059]  [MGI Ref ID J:108375]

Martin A; Tsui HW; Tsui FW. 1999. SHP-1 variant proteins are absent in motheaten mice despite presence of splice variant transcripts with open reading frames. Mol Immunol 36(15-16):1029-41. [PubMed: 10698306]  [MGI Ref ID J:60297]

McCoy KL; Clagett J; Rosse C. 1985. Effects of the motheaten gene on murine B-cell production. Exp Hematol 13(6):554-9. [PubMed: 3873348]  [MGI Ref ID J:7856]

McCoy KL; Nielson K; Clagett J. 1984. Spontaneous production of colony-stimulating activity by splenic Mac-1 antigen-positive cells from autoimmune motheaten mice. J Immunol 132(1):272-6. [PubMed: 6361123]  [MGI Ref ID J:7274]

Mlinaric-Rascan I; Asa SL; Siminovitch KA. 1994. Increased expression of the stefin A cysteine proteinase inhibitor occurs in the myelomonocytic cell-infiltrated tissues of autoimmune motheaten mice. Am J Pathol 145(4):902-12. [PubMed: 7943179]  [MGI Ref ID J:20878]

Nakamura MC; Niemi EC; Fisher MJ; Shultz LD; Seaman WE; Ryan JC. 1997. Mouse Ly-49A interrupts early signaling events in natural killer cell cytotoxicity and functionally associates with the SHP-1 tyrosine phosphatase. J Exp Med 185(4):673-84. [PubMed: 9034146]  [MGI Ref ID J:38943]

Paulson RF; Vesely S; Siminovitch KA; Bernstein A. 1996. Signalling by the W/Kit receptor tyrosine kinase is negatively regulated in vivo by the protein tyrosine phosphatase Shp1. Nat Genet 13(3):309-15. [PubMed: 8673130]  [MGI Ref ID J:34290]

Sathish JG; Walters J; Luo JC; Johnson KG; Leroy FG; Brennan P; Kim KP; Gygi SP; Neel BG; Matthews RJ. 2004. CD22 is a functional ligand for SH2 domain-containing protein-tyrosine phosphatase-1 in primary T cells. J Biol Chem 279(46):47783-91. [PubMed: 15364920]  [MGI Ref ID J:118518]

Scribner CL; Hansen CT; Klinman DM; Steinberg AD. 1987. The interaction of the xid and me genes. J Immunol 138(11):3611-7. [PubMed: 2884254]  [MGI Ref ID J:30994]

Shultz LD; Bailey CL; Coman DR. 1983. Hematopoietic stem cell function in motheaten mice. Exp Hematol 11(7):667-80. [PubMed: 6350031]  [MGI Ref ID J:7162]

Shultz LD; Green MC. 1976. Motheaten, an immunodeficient mutant of the mouse. II. Depressed immune competence and elevated serum immunoglobulins. J Immunol 116(4):936-43. [PubMed: 56406]  [MGI Ref ID J:5624]

Shultz LD; Rajan TV; Greiner DL. 1997. Severe defects in immunity and hematopoiesis caused by SHP-1 protein-tyrosine-phosphatase deficiency. Trends Biotechnol 15(8):302-7. [PubMed: 9263478]  [MGI Ref ID J:42157]

Shultz LD; Schweitzer PA; Rajan TV; Yi T; Ihle JN; Matthews RJ; Thomas ML; Beier DR. 1993. Mutations at the murine motheaten locus are within the hematopoietic cell protein-tyrosine phosphatase (Hcph) gene. Cell 73(7):1445-54. [PubMed: 8324828]  [MGI Ref ID J:14935]

Sidman CL; Shultz LD; Unanue ER. 1978. The mouse mutant motheaten. II. Functional studies of the immune system. J Immunol 121(6):2399-404. [PubMed: 363947]  [MGI Ref ID J:6065]

Sundberg JP (ed.). 1994. . In: Handbook of Mouse Mutations with Skin and Hair Abnormalities: Animal Models and Biomedical Tools. CRC Press, Boca Raton.  [MGI Ref ID J:30359]

Takeoka Y; Chen SY; Boyd RL; Tsuneyama K; Taguchi N; Morita S; Yago H; Suehiro S; Ansari AA; Shultz LD; Gershwin ME. 1997. A comparative analysis of the murine thymic microenvironment in normal, autoimmune, and immunodeficiency states. Dev Immunol 5(2):79-89. [PubMed: 9587708]  [MGI Ref ID J:44504]

Tsui FW; Tsui HW. 1994. Molecular basis of the motheaten phenotype. Immunol Rev 138:185-206. [PubMed: 8070815]  [MGI Ref ID J:18549]

Tsui HW; Siminovitch KA; de Souza L; Tsui FW. 1993. Motheaten and viable motheaten mice have mutations in the haematopoietic cell phosphatase gene. Nat Genet 4(2):124-9. [PubMed: 8348149]  [MGI Ref ID J:11892]

Umeda S; Beamer WG; Takagi K; Naito M; Hayashi S; Yonemitsu H; Yi T; Shultz LD. 1999. Deficiency of SHP-1 protein-tyrosine phosphatase activity results in heightened osteoclast function and decreased bone density. Am J Pathol 155(1):223-33. [PubMed: 10393854]  [MGI Ref ID J:108187]

Ward JM. 1978. Pulmonary pathology of the motheaten mouse. Vet Pathol 15(2):170-8. [PubMed: 664185]  [MGI Ref ID J:5999]

Wishcamper CA; Coffin JD; Lurie DI. 2001. Lack of the protein tyrosine phosphatase SHP-1 results in decreased numbers of glia within the motheaten (me/me) mouse brain. J Comp Neurol 441(2):118-33. [PubMed: 11745639]  [MGI Ref ID J:72655]

Yang W; Mckenna SD; Jiao H; Tabrizi M; Lynes MA; Shultz LD; Yi T. 1998. SHP-1 deficiency in B-lineage cells is associated with heightened lyn protein expression and increased lyn kinase activity. Exp Hematol 26(12):1126-32. [PubMed: 9808051]  [MGI Ref ID J:51142]

Zhao J; Lurie DI. 2004. Cochlear ablation in mice lacking SHP-1 results in an extended period of cell death of anteroventral cochlear nucleus neurons. Hear Res 189(1-2):63-75. [PubMed: 14987753]  [MGI Ref ID J:88807]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations             View   International   Pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $1900.00
*Price(s) in US dollars ($)

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery of Strains Needing Progeny Testing.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two untested males and two untested females (two pairs) will be recovered, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the overall recovery time to approximately 25 weeks. However, all pups recovered will be sent.

    Progeny testing is required to identify the genotype of mice of this strain, as a genotyping assay is not available. This type of testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. We can perform the progeny testing for you as a service or we can ship all recovered animals (at least two untested pairs) to you for progeny testing at your facility. If you perform the progeny testing, there is NO guarantee that a carrier will be identified. If we perform progeny testing as a service, additional breeding time will be required. In this case, when a male and female (one pair) are identified that carry the mutation, they and their offspring will be shipped. Delivery time for strains requiring progeny testing often exceeds 25 weeks and may take 12 months or more due to the difficulties in breeding some strains. The progeny testing cost is in addition to the recovery cost and is based on the number of boxes used and the time taken to produce the mice identified as carrying the mutation. Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Please contact Customer Service for more information on the cost of progeny testing for a strain: Tel: 1-800-422-6423 or 1-207-288-5845.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • This strain is included in the Mouse Mutant Resource collection.
  • Genomic DNA is available for this strain from the Mouse DNA Resource.

Pricing for International shipping destinations             View   USA, Canada and Mexico   Pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $2470.00
*Price(s) in US dollars ($)

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery of Strains Needing Progeny Testing.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two untested males and two untested females (two pairs) will be recovered, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the overall recovery time to approximately 25 weeks. However, all pups recovered will be sent.

    Progeny testing is required to identify the genotype of mice of this strain, as a genotyping assay is not available. This type of testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. We can perform the progeny testing for you as a service or we can ship all recovered animals (at least two untested pairs) to you for progeny testing at your facility. If you perform the progeny testing, there is NO guarantee that a carrier will be identified. If we perform progeny testing as a service, additional breeding time will be required. In this case, when a male and female (one pair) are identified that carry the mutation, they and their offspring will be shipped. Delivery time for strains requiring progeny testing often exceeds 25 weeks and may take 12 months or more due to the difficulties in breeding some strains. The progeny testing cost is in addition to the recovery cost and is based on the number of boxes used and the time taken to produce the mice identified as carrying the mutation. Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Please contact Customer Service for more information on the cost of progeny testing for a strain: Tel: 1-800-422-6423 or 1-207-288-5845.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • This strain is included in the Mouse Mutant Resource collection.
  • Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

  Control
   Untyped from the colony
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering and Purchasing Information

      Purchasing Information
      JAX® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form


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