Strain Name: |
B6C3Fe a/a-Wnt1sw/J |
|---|---|
Stock Number: |
000243 |
Availability: | Repository-Cryopreserved |
Price and Supply Information | |
General Terms and Conditions |
| Genes & Alleles | Wnt1; Wnt1sw; a; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Spontaneous Mutation Species laboratory mouse Generation F3NE16p+F2 Strain Description
Mice homozygous for the swaying spontaneous mutation (Wnt1sw) sway to one side or the other when attempting to move and may then pivot clockwise or counterclockwise around their rear legs. Homozygous mutant mice display marked ataxia and hypertonia that is probably attributable to malformations of the anterior vermis of the cerebellum and of the colliculi. The cerebellum is divided on the midline by a deep dorsal sagittal fissure extending from the leptomeninges down to the level of the fourth ventricle. The probable cause is failure of the midline fusion of the cerebellum.Strain Development
The mutation swaying (Wnt1sw) arose spontaneously in a cross between mahoganoid (Mgrn1md), which arose in C3H/HeJ, and downless Jackson (Edardl-J), which arose in a cross of two silver stocks at the Jackson Laboratory in 1966. It was sibling mated for 2 generations then a homozygous swaying female was crossed to a C57BL/6J male. The swaying mutation was backcrossed to C57BL/6J for 5 more generations either by mating a progeny tested heterozygous (sw) male or female to a C57BL/6J or by ovary transplant. In 1971 a C57BL/6J velvet coat (Ve) male at N13 was crossed to a host carrying a C57BL/6J sw/sw ovary. The pairs, Ve +/+ sw, were mated at N7 and the stock was maintained in this manner to N7F10. Because of poor breeding a cross to C3HeB/FeJ-a/a was made with a sw/sw ovary transplant. The stock was then maintained by the cross-intercross method using a homozyogus ovary transplant host crossed with a B6C3Fe-a/a F1 male. The stock was frozen in 1982 by mating heterozygous males to heterozygous females at N13. It was thawed and re-frozen in 1990 by intercrossing heterozygotes and again in 2003 by IVF using homozygous sperm and +/? females at [F3NE16p]+F2.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Wnt1sw | ||
|---|---|---|---|
| Allele Name | swaying | ||
| Common Name(s) | sw; | ||
| Strain of Origin | STOCK Atrnmg Edardl-J | ||
| Gene Symbol and Name | Wnt1, wingless-related MMTV integration site 1 | ||
| Chromosome | 15 | ||
| Gene Common Name(s) | INT1; Int-1; Wnt-1; mammary tumor integration site 1; sw; swaying; | ||
| General Note | A mutation that arose spontaneously in a linkage test cross was given the name swaying because homozygotes sway to one side or the other when attempting to move and may then pivot clockwise or counterclockwise around their rear legs. They swim poorly and often roll over (J:15063). Their marked ataxia and hypertonia are probably attributable to malformations of the anterior vermis of the cerebellum and of the colliculi. The anterior parts of the cerebellar cortex are badly scrambled, and the white matteris fused directly with the superior colliculus. Despite the severe cortical malformation, the individual cell types in the cortex bear normal anatomic relations to one another (J:30722). However, the cerebellum is divided on the midline by a deep dorsal sagittal fissure extending from the leptomeninges down to the level of the fourth ventricle. The probable cause is failure of midline fusion of the cerebellum. At 15-16 days of gestation, the cerebellar hemispheres have begun to fuse, but are still separated by sagittal grooves. These grooves disappear over the next few days of gestation in normal embryos, but not in Wnt1sw/Wnt1sw mice (J:1970). Normal development of the boundary between mesencephalon and metencephalon is never established in homozygotes for Wnt1sw (J:29060). | ||
| Molecular Note | The mutation is a deletion of 1 guanosine residue from a run of 4 consecutive guanosines beginning at genomic nucleotide 1888. This mutation is predicted to cause a frameshift mutation resulting in a stop codon 10 codons downstream from the deletion. The predicted protein would lack critical amino acids for biological activity. [MGI Ref ID J:2964] | ||
| Allele Symbol | a | ||
| Allele Name | nonagouti | ||
Related Strains
Strains carrying a allele
View Strains carrying a (102 strains)
002865 B6CBA-Tg(Wnt1-lacZ)206Amc/J 002870 B6SJL-Tg(Wnt1)1Hev/J 002934 FVB.Cg-Tg(Wnt1)1Hev/J 003829 STOCK Tg(Wnt1-cre)11Rth Tg(Wnt1-GAL4)11Rth/J
Research Applications
This mouse can be used to support research in many areas including:Wnt1sw related
Developmental Biology Research
Neural Tube Defects
Neurobiology Research
Ataxia (Movement) Defects
Cerebellar Defects
Neural Tube Defects
References
Additional ReferencesPrice and Supply Information
| Strain Name: | B6C3Fe a/a-Wnt1sw/J |
| Stock Number: | 000243 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
USA, Canada and Mexico
| Price(s) in US dollars ($) | |||||
|---|---|---|---|---|---|
| Cryorecovery Fee | $1900.00 | ||||
| Cryopreserved Embryos Fee | $1600.00 | ||||
Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery of Strains Needing Progeny Testing. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two untested males and two untested females (two pairs) will be recovered, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the overall recovery time to approximately 25 weeks. However, all pups recovered will be sent. Progeny testing is required to identify the genotype of mice of this strain, as a genotyping assay is not available. This type of testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. We can perform the progeny testing for you as a service or we can ship all recovered animals (at least two untested pairs) to you for progeny testing at your facility. If you perform the progeny testing, there is NO guarantee that a carrier will be identified. If we perform progeny testing as a service, additional breeding time will be required. In this case, when a male and female (one pair) are identified that carry the mutation, they and their offspring will be shipped. Delivery time for strains requiring progeny testing often exceeds 25 weeks and may take 12 months or more due to the difficulties in breeding some strains. The progeny testing cost is in addition to the recovery cost and is based on the number of boxes used and the time taken to produce the mice identified as carrying the mutation. Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Please contact Customer Service for more information on the cost of progeny testing for a strain: Tel: 1-800-422-6423 or 1-207-288-5845. Cryorecovery to establish a Dedicated Supply for greater quantities of mice |
| Licensing | See General Terms and Conditions below |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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