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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Background Strain AEJ/GnLe Donor Strain PCT multiple recessive testing stock Generation N5 F55
Generation DefinitionsAppearance
nonagouti black with normal sized feet and limbs
Related Genotype: a Gdf5bp-H/ae + or a ?/? +
nonagouti black with shortened toes and limbs
Related Genotype: a Gdf5bp-H/a ?Gdf5bp-H
extreme nonagouti black (no lightened hair around the ears, perineum or nipples) with normal feet and limbs
Related Genotype: ae +/ae ?Development
The ae mutation was discovered by W.F. Hollander and J.W. Gowen among descendants of an irradiated male albino mouse of the S strain then maintained at Iowa State College and a female of the multiple-recessive P testing stock. A single black, typical non-agouti male produced by these matings was bred to three AY/A+ females (of unidentified background), yielding five male offspring that survived to maturity, three of which proved fertile. Each of these was bred to a female homozygous for the black-and-tan (a t) allele, which is recessive to both AY and A+. Black-and-tan offspring of this cross were bred among themselves, as were progeny of the latter crosses. In one of the resultant lines, the black mice had an extreme non-agouti phenotype, having no yellow hairs. This line contributed to the background of the inbred strain AEJ, which carries ae. Gdf5bp-H occurred spontaneously in the PCT multiple recessive stock at Harwell, U.K. It was backcrossed onto AEJ/GnLe-ae for 5 generations, and the stock has since been maintained by brother-sister inbreeding.
Control Information
| Control | ||
|---|---|---|
| Untyped from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Gdf5bp-H allele
000596 STOCK T(2;11)30H/+ x AEJ-a Gdf5bp-H/J or A/J-a Gdf5bp-J/J View Strains carrying Gdf5bp-H (1 strain)
000202 AEJ/Gn-bd/J 000199 AEJ/GnLeJ
002239 B6 x BALB/cJ-Gdf5bp-3J/J 000596 STOCK T(2;11)30H/+ x AEJ-a Gdf5bp-H/J or A/J-a Gdf5bp-J/J
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Gdf5bp-H/Gdf5bp-H
involves: PCT stock * SWISS albino
- skeleton phenotype
- abnormal cartilage development
- fibular cartilage obtained within 6 hours of birth shows decreased chondrocyte protein synthesis, aberrant metabolism of collagen and chondroitin sulfate, and morphological abnormalities (MGI Ref ID J:166507)
- abnormal long bone epiphyseal plate morphology
- epiphyseal cartilage of the fibula obtained within 6 hours of birth is shorter than normal, unscuptured, and shows homogeneous distribution of chondrocytes throughout the rudiment, rather than the three distinct cellular zones of the normal control (MGI Ref ID J:166507)
- delayed endochondral bone ossification
- evident in fibulae assessed within 6 hours of birth (MGI Ref ID J:166507)
- limbs/digits/tail phenotype
- abnormal long bone epiphyseal plate morphology
- epiphyseal cartilage of the fibula obtained within 6 hours of birth is shorter than normal, unscuptured, and shows homogeneous distribution of chondrocytes throughout the rudiment, rather than the three distinct cellular zones of the normal control (MGI Ref ID J:166507)
Gdf5bp-H/Gdf5bp-H
involves: PCT
- limbs/digits/tail phenotype
- abnormal limb morphology
- skeleton phenotype
- abnormal cartilage development
- shortened long bones are the result of retardation of growth and differentiation of cartilage cells formed from postaxial mesenchyme (MGI Ref ID J:172114)
- abnormal endochondral bone ossification
- differentiation of the femur and fibula is retarded compared with normal development (MGI Ref ID J:172114)
- abnormal femur morphology
- short femur
- observed at birth (MGI Ref ID J:172114)
- abnormal fibula morphology
- short fibula
- observed at birth (MGI Ref ID J:172114)
- abnormal tibia morphology
- short tibia
- observed at birth (MGI Ref ID J:172114)
ae/ae
(P x S)F1This mouse can be used to support research in many areas including:Gdf5bp-H related
ae relatedCell Biology Research
Defects in Extracellular Matrix Molecules
Developmental Biology Research
Defects in Extracellular Matrix Molecules
Growth Defects
Growth Defects (homozygous)
Skeletal Defects
Mouse/Human Gene Homologs
acromesomelic dysplasia, Hunter-Thompson Type
Dermatology Research
Color and White Spotting Defects
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Gdf5bp-H | ||
|---|---|---|---|
| Allele Name | brachypodism Harwell | ||
| Allele Type | Spontaneous | ||
| Common Name(s) | bp-H; | ||
| Strain of Origin | PCT | ||
| Gene Symbol and Name | Gdf5, growth differentiation factor 5 | ||
| Chromosome | 2 | ||
| Gene Common Name(s) | BMP14; CDMP-1; CDMP1; LAP4; OS5; SYNS2; bp; brachypod Japan; brachypodism; brp; cartilage-derived morphogenetic protein-1; | ||
| General Note | This remutation arose spontaneously at Harwell in the PCT multiple recessive stock (J:13391). It is essentially identical in effect with Gdf5bp, and has been used extensively in studies of brachypodism. Dissociated mesoderm cells from 12-day hindlimb buds of Gdfbp-H homozygous mutant embryos make fewer and smaller condensations than those of control embryos and chondrogenesis is delayed (J:12041). Cells of the limb mesenchyme are more adhesive than in normal embryos (J:6104). Cell aggregation and cartilaginous nodule formation were determined in cultures of various combinations of cells from different stages and parts of limb buds of 10- to 13-day embryos. In limb buds from mice homozygous for Gdf5bp-H, there is reduced ability of a specific mesenchyme cell population to provide inductive stimulus for chondrogenesis. This cell-cell interaction results in a lower proportion of chondrogenic cells and a higher proportion of more mature non-chondrogenic cells. A factor produced by the femur and fibula or by the postaxial part of the hindlimb bud of 13-day Gdf5bp-H homozygous mutant mice inhibits the growth in vitro of tibias of 13-day wild-typeembryos (J:5444). Although tibias from 13-day mutant embryos grow normally in standard cultures, addition of a factor from the postaxial part of limb buds from 11- to 12-day wild-type mice causes reduces growth of mutant but not of wild-type 13-day tibias (J:11997). In an in vitro system which supports autonomous development of individual limb elements, the proximal-to-distal increase of severity of cartilage reduction is intensified in explants from homozygous mutant mice. (J:942). | ||
| Molecular Note | This allele was identified by a noncomplementation test with Gdf5bp. [MGI Ref ID J:13391] | ||
| Allele Symbol | ae | ||
| Allele Name | extreme nonagouti | ||
| Allele Type | Radiation induced | ||
| Strain of Origin | (P x S)F1 | ||
| Gene Symbol and Name | a, nonagouti | ||
| Chromosome | 2 | ||
| Gene Common Name(s) | AGSW; AGTI; AGTIL; ASP; As; SHEP9; agouti; agouti signal protein; agouti suppressor; | ||
| General Note | The ae mutation was found among descendants of an irradiated mouse. ae is recessive to all other alleles except al. | ||
References
References provided by MGI
Additional References
Bultman SJ; Michaud EJ; Woychik RP. 1992. Molecular characterization of the mouse agouti locus. Cell 71(7):1195-204. [PubMed: 1473152] [MGI Ref ID J:3523]
Hustad CM; Perry WL; Siracusa LD; Rasberry C; Cobb L; Cattanach BM; Kovatch R; Copeland NG; Jenkins NA. 1995. Molecular genetic characterization of six recessive viable alleles of the mouse agouti locus. Genetics 140(1):255-65. [PubMed: 7635290] [MGI Ref ID J:24934]
Miller MW; Duhl DM; Vrieling H; Cordes SP; Ollmann MM; Winkes BM; Barsh GS. 1993. Cloning of the mouse agouti gene predicts a secreted protein ubiquitously expressed in mice carrying the lethal yellow mutation. Genes Dev 7(3):454-67. [PubMed: 8449404] [MGI Ref ID J:4186]
Vrieling H; Duhl DM; Millar SE; Miller KA; Barsh GS. 1994. Differences in dorsal and ventral pigmentation result from regional expression of the mouse agouti gene. Proc Natl Acad Sci U S A 91(12):5667-71. [PubMed: 8202545] [MGI Ref ID J:18750]
Gdf5bp-H relatedae relatedBugrilova RS; Konyukhov BV. 1978. Regulator of the expression of the gene brachypodism-H in the mouse. Sov Genet 14:1272-1278. [MGI Ref ID J:11997]
Duke J; Elmer WA. 1978. Cell adhesion and chondrogenesis in brachypod mouse limb mesenchyme: fragment fusion studies. J Embryol Exp Morphol 48:161-8. [PubMed: 744946] [MGI Ref ID J:6104]
Duke J; Elmer WA. 1977. Effect of the brachypod mutation on cell adhesion and chondrogenesis in aggregates of mouse limb mesenchyme. J Embryol Exp Morphol 42:209-217. [MGI Ref ID J:12041]
Gruneberg H; Lee AJ. 1973. The anatomy and development of brachypodism in the mouse. J Embryol Exp Morphol 30(1):119-41. [PubMed: 4729943] [MGI Ref ID J:5372]
Konyukhov BV; Ginter EK. 1966. A study of the action of the brachypodism-H gene on development of the long bones of the hind limbs in the mouse. Folia Biol (Praha) 12(3):199-206. [PubMed: 5949834] [MGI Ref ID J:172114]
Kwasigroch TE; Curtis SK; Knudsen TB; Barrach HJ; Elmer WA. 1992. Morphological analysis of abnormal digital chondrogenesis in the brachypod (bpH) mouse limb in organ culture. Anat Embryol (Berl) 185(4):307-15. [PubMed: 1609959] [MGI Ref ID J:942]
Lyon MF. 1958. brachypody, bp Mouse News Lett 19:22. [MGI Ref ID J:13391]
Owens EM; Solursh M. 1983. Accelerated maturation of limb mesenchyme by the BrachypodH mouse mutation. Differentiation 24(2):145-8. [PubMed: 6309591] [MGI Ref ID J:7163]
Owens EM; Solursh M. 1982. Cell-cell interaction by mouse limb cells during in vitro chondrogenesis: analysis of the brachypod mutation. Dev Biol 91(2):376-88. [PubMed: 7095270] [MGI Ref ID J:6808]
Pleskova MV; Rodionov VM; Bugrilova RS; Konyukhov BV. 1974. The partial purification of growth-inhibiting factor of the brachypodism-H mouse embryos. Dev Biol 37(2):417-21. [PubMed: 4826284] [MGI Ref ID J:5444]
Rhodes RK; Elmer WA. 1975. Aberrant metabolism of matrix components in neonatal fibular cartilage of brachypod (bpH) mice. Dev Biol 46(1):14-27. [PubMed: 125666] [MGI Ref ID J:166507]
Barsh GS; Epstein CJ. 1989. Physical and genetic characterization of a 75-kilobase deletion associated with al, a recessive lethal allele at the mouse agouti locus. Genetics 121(4):811-8. [PubMed: 2566558] [MGI Ref ID J:9799]
Candille SI; Raamsdonk CD; Chen C; Kuijper S; Chen-Tsai Y; Russ A; Meijlink F; Barsh GS. 2004. Dorsoventral patterning of the mouse coat by tbx15. PLoS Biol 2(1):E3. [PubMed: 14737183] [MGI Ref ID J:87455]
Hollander WF; Gowen JW. 1956. An extreme non-agouti mutant in the mouse. J Hered 47:221-224. [MGI Ref ID J:13070]
Hustad CM; Perry WL; Siracusa LD; Rasberry C; Cobb L; Cattanach BM; Kovatch R; Copeland NG; Jenkins NA. 1995. Molecular genetic characterization of six recessive viable alleles of the mouse agouti locus. Genetics 140(1):255-65. [PubMed: 7635290] [MGI Ref ID J:24934]
Lyon MF; Fisher G; Glenister PH. 1985. A recessive allele of the mouse agouti locus showing lethality with yellow, Ay. Genet Res 46(1):95-9. [PubMed: 4065569] [MGI Ref ID J:8093]
Poole TW. 1975. Dermal-epidermal interactions and the action of alleles at the agouti locus in the mouse. Dev Biol 42(2):203-10. [PubMed: 1090472] [MGI Ref ID J:5519]
Silvers WK. 1979. The Coat Colors of Mice; A Model for Mammalian Gene Action and Interaction. In: The Coat Colors of Mice. Springer-Verlag, New York. [MGI Ref ID J:78801]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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 |
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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|
|
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Untyped from the colony | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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