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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Background Strain AEJ/GnLe Donor Strain PCT multiple recessive testing stock Generation N5 F55 Appearance
nonagouti black with normal sized feet and limbs
Related Genotype: a Gdf5bp-H/ae + or a ?/? +
nonagouti black with shortened toes and limbs
Related Genotype: a Gdf5bp-H/a ?Gdf5bp-H
extreme nonagouti black (no lightened hair around the ears, perineum or nipples) with normal feet and limbs
Related Genotype: ae +/ae ?Development
The ae mutation was discovered by W.F. Hollander and J.W. Gowen among descendants of an irradiated male albino mouse of the S strain then maintained at Iowa State College and a female of the multiple-recessive P testing stock. A single black, typical non-agouti male produced by these matings was bred to three AY/A+ females (of unidentified background), yielding five male offspring that survived to maturity, three of which proved fertile. Each of these was bred to a female homozygous for the black-and-tan (a t) allele, which is recessive to both AY and A+. Black-and-tan offspring of this cross were bred among themselves, as were progeny of the latter crosses. In one of the resultant lines, the black mice had an extreme non-agouti phenotype, having no yellow hairs. This line contributed to the background of the inbred strain AEJ, which carries ae. Gdf5bp-H occurred spontaneously in the PCT multiple recessive stock at Harwell, U.K. It was backcrossed onto AEJ/GnLe-ae for 5 generations, and the stock has since been maintained by brother-sister inbreeding.
Control Information
| Control | ||
|---|---|---|
| Untyped from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Gdf5bp-H allele
000596 STOCK T(2;11)30H/+ x AEJ-a Gdf5bp-H/J or A/J-a Gdf5bp-J/J View Strains carrying Gdf5bp-H (1 strain)
000202 AEJ/Gn-bd/J 000199 AEJ/GnLeJ
002239 B6 x BALB/cJ-Gdf5bp-3J/J 000596 STOCK T(2;11)30H/+ x AEJ-a Gdf5bp-H/J or A/J-a Gdf5bp-J/J
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
ae/ae
(P x S)F1
- pigmentation phenotype
- abnormal coat/hair pigmentation (MGI Ref ID J:13070)
- darkened coat color (MGI Ref ID J:13070)
- black is more intense than homozygous non-agouti
- no yellow hairs in the ears or around nipples and perineum as found in homozygous non-agouti mice
- skin/coat/nails phenotype
- abnormal coat/hair pigmentation (MGI Ref ID J:13070)
- darkened coat color (MGI Ref ID J:13070)
- black is more intense than homozygous non-agouti
- no yellow hairs in the ears or around nipples and perineum as found in homozygous non-agouti mice
This mouse can be used to support research in many areas including:Gdf5bp-H related
ae relatedDevelopmental Biology Research
Growth Defects
Mouse/Human Gene Homologs
acromesomelic dysplasia, Hunter-Thompson Type
Dermatology Research
Color and White Spotting Defects
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Gdf5bp-H | ||
|---|---|---|---|
| Allele Name | brachypodism Harwell | ||
| Allele Type | Spontaneous | ||
| Strain of Origin | PCT | ||
| Gene Symbol and Name | Gdf5, growth differentiation factor 5 | ||
| Chromosome | 2 | ||
| Gene Common Name(s) | BMP14; CDMP-1; CDMP1; LAP4; OS5; SYNS2; bp; brachypod Japan; brachypodism; brp; cartilage-derived morphogenetic protein-1; | ||
| General Note | This remutation arose spontaneously at Harwell in the PCT multiple recessive stock (J:13391). It is essentially identical in effect with Gdf5bp, and has been used extensively in studies of brachypodism. Dissociated mesoderm cells from 12-day hindlimb buds of Gdfbp-H homozygous mutant embryos make fewer and smaller condensations than those of control embryos and chondrogenesis is delayed (J:12041). Cells of the limb mesenchyme are more adhesive than in normal embryos (J:6104). Cell aggregation and cartilaginous nodule formation were determined in cultures of various combinations of cells from different stages and parts of limb buds of 10- to 13-day embryos. In limb buds from mice homozygous for Gdf5bp-H, there is reduced ability of a specific mesenchyme cell population to provide inductive stimulus for chondrogenesis. This cell-cell interaction results in a lower proportion of chondrogenic cells and a higher proportion of more mature non-chondrogenic cells. A factor produced by the femur and fibula or by the postaxial part of the hindlimb bud of 13-day Gdf5bp-H homozygous mutant mice inhibits the growth in vitro of tibias of 13-day wild-typeembryos (J:5444). Although tibias from 13-day mutant embryos grow normally in standard cultures, addition of a factor from the postaxial part of limb buds from 11- to 12-day wild-type mice causes reduces growth of mutant but not of wild-type 13-day tibias (J:11997). In an in vitro system which supports autonomous development of individual limb elements, the proximal-to-distal increase of severity of cartilage reduction is intensified in explants from homozygous mutant mice. (J:942). | ||
| Allele Symbol | ae | ||
| Allele Name | extreme nonagouti | ||
| Allele Type | Radiation induced | ||
| Strain of Origin | (P x S)F1 | ||
| Gene Symbol and Name | a, nonagouti | ||
| Chromosome | 2 | ||
| Gene Common Name(s) | AGSW; AGTI; AGTIL; ASP; As; MGC126092; MGC126093; SHEP9; agouti; agouti signal protein; agouti suppressor; | ||
| General Note | The ae mutation was found among descendants of an irradiated mouse. ae is recessive to all other alleles except al. | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Genotyping resources and troubleshooting
References
References
Additional References
Bultman SJ; Michaud EJ; Woychik RP. 1992. Molecular characterization of the mouse agouti locus. Cell 71(7):1195-204. [PubMed: 1473152] [MGI Ref ID J:3523]
Hustad CM; Perry WL; Siracusa LD; Rasberry C; Cobb L; Cattanach BM; Kovatch R; Copeland NG; Jenkins NA. 1995. Molecular genetic characterization of six recessive viable alleles of the mouse agouti locus. Genetics 140(1):255-65. [PubMed: 7635290] [MGI Ref ID J:24934]
Miller MW; Duhl DM; Vrieling H; Cordes SP; Ollmann MM; Winkes BM; Barsh GS. 1993. Cloning of the mouse agouti gene predicts a secreted protein ubiquitously expressed in mice carrying the lethal yellow mutation. Genes Dev 7(3):454-67. [PubMed: 8449404] [MGI Ref ID J:4186]
Vrieling H; Duhl DM; Millar SE; Miller KA; Barsh GS. 1994. Differences in dorsal and ventral pigmentation result from regional expression of the mouse agouti gene. Proc Natl Acad Sci U S A 91(12):5667-71. [PubMed: 8202545] [MGI Ref ID J:18750]
Gdf5bp-H relatedae relatedBugrilova RS; Konyukhov BV. 1978. Regulator of the expression of the gene brachypodism-H in the mouse. Sov Genet 14:1272-1278. [MGI Ref ID J:11997]
Duke J; Elmer WA. 1978. Cell adhesion and chondrogenesis in brachypod mouse limb mesenchyme: fragment fusion studies. J Embryol Exp Morphol 48:161-8. [PubMed: 744946] [MGI Ref ID J:6104]
Duke J; Elmer WA. 1977. Effect of the brachypod mutation on cell adhesion and chondrogenesis in aggregates of mouse limb mesenchyme. J Embryol Exp Morphol 42:209-217. [MGI Ref ID J:12041]
Kwasigroch TE; Curtis SK; Knudsen TB; Barrach HJ; Elmer WA. 1992. Morphological analysis of abnormal digital chondrogenesis in the brachypod (bpH) mouse limb in organ culture. Anat Embryol (Berl) 185(4):307-15. [PubMed: 1609959] [MGI Ref ID J:942]
Lyon MF. 1958. brachypody, bp Mouse News Lett 19:22. [MGI Ref ID J:13391]
Owens EM; Solursh M. 1983. Accelerated maturation of limb mesenchyme by the BrachypodH mouse mutation. Differentiation 24(2):145-8. [PubMed: 6309591] [MGI Ref ID J:7163]
Owens EM; Solursh M. 1982. Cell-cell interaction by mouse limb cells during in vitro chondrogenesis: analysis of the brachypod mutation. Dev Biol 91(2):376-88. [PubMed: 7095270] [MGI Ref ID J:6808]
Pleskova MV; Rodionov VM; Bugrilova RS; Konyukhov BV. 1974. The partial purification of growth-inhibiting factor of the brachypodism-H mouse embryos. Dev Biol 37(2):417-21. [PubMed: 4826284] [MGI Ref ID J:5444]
Barsh GS; Epstein CJ. 1989. Physical and genetic characterization of a 75-kilobase deletion associated with al, a recessive lethal allele at the mouse agouti locus. Genetics 121(4):811-8. [PubMed: 2566558] [MGI Ref ID J:9799]
Candille SI; Raamsdonk CD; Chen C; Kuijper S; Chen-Tsai Y; Russ A; Meijlink F; Barsh GS. 2004. Dorsoventral patterning of the mouse coat by tbx15. PLoS Biol 2(1):E3. [PubMed: 14737183] [MGI Ref ID J:87455]
Hollander WF; Gowen JW. 1956. An extreme non-agouti mutant in the mouse. J Hered 47:221-224. [MGI Ref ID J:13070]
Hustad CM; Perry WL; Siracusa LD; Rasberry C; Cobb L; Cattanach BM; Kovatch R; Copeland NG; Jenkins NA. 1995. Molecular genetic characterization of six recessive viable alleles of the mouse agouti locus. Genetics 140(1):255-65. [PubMed: 7635290] [MGI Ref ID J:24934]
Lyon MF; Fisher G; Glenister PH. 1985. A recessive allele of the mouse agouti locus showing lethality with yellow, Ay. Genet Res 46(1):95-9. [PubMed: 4065569] [MGI Ref ID J:8093]
Poole TW. 1975. Dermal-epidermal interactions and the action of alleles at the agouti locus in the mouse. Dev Biol 42(2):203-10. [PubMed: 1090472] [MGI Ref ID J:5519]
Silvers WK. 1979. The Coat Colors of Mice; A Model for Mammalian Gene Action and Interaction. In: The Coat Colors of Mice. Springer-Verlag, New York. [MGI Ref ID J:78801]
Health & husbandry
Health & Colony Maintenance Information
Currently there no information available for this strain. This may be due to the supply level of this strain.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Untyped from the colony | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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JAX® Mice, Products & Services Conditions of Use
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