JAX Mice Database - 000262 LS/LeJ

Strain Name:	LS/LeJ
Stock Number:	000262
Availability:	Repository-Cryopreserved
Common Names:	Lethal Spotting;

Description
Phenotype
Genes & alleles
Genotyping
Health & husbandry
References
Purchasing information
Terms of Use

Description

Strain Information

Type Spontaneous Mutation;
Additional information on Genetically Engineered Mutant Mice.

Type Inbred Strain;
Additional information on Inbred Strains.

Species laboratory mouse

Appearance
black and tan
Related Genotype: a^t Edn3^ls/a^t +

black with white spots, early lethal
Related Genotype: a^t Edn3^ls/a^t Edn3^ls
Important Note
This strain is homozygous for a^t and segregating for Edn3^ls.
Description
Mice homozygous for the lethal spotting mutation (Edn3^ls) resemble piebald mice (Ednrb^s/Ednrb^s) mice. Homozygous mutant mice exhibit considerable white spotting and like homozygotes for the piebald-lethal allele (Ednrb^s-l) usually develop megacolon. Lethal spotting mutant mice usually die in the third week of life from the megacolon abnormality, which is associated with deficiency of intrinsic ganglion cells in the lower colon. A few homozygotes may survive and breed. This strain is also homozygous for the black and tan coat color mutation (a^t).

Control Information

	Control
	Heterozygote from the colony

Considerations for Choosing Controls

Related Strains

Strains carrying a^t allele

000477 B10.PA-Pldn^pa H3^e a^t/SnJ

000419 B10.UW-H3^b we Pax1^un a^t/SnJ

000065 B6C3Fe a/a-we Pax1^un a^t/J

View Strains carrying a^t (3 strains)

Strains carrying other alleles of Edn3

002516 129-Edn3^tm1Ywa/J

View Strains carrying other alleles of Edn3 (1 strain)

Strains carrying other alleles of a

003301 (C57BL/6J x C3H-Eya1^bor)F1/J

000251 AEJ.Cg-a^e +/a Gdf5^bp-H/J

000202 AEJ/Gn-bd/J

000199 AEJ/GnLeJ

000427 B10.CE-H13^b A^w/(30NX)SnJ

000420 B10.LP-H13^b A^w/Sn

003879 B10;TFLe-a/a T tf/+ tf/J

001538 B6 x B6C3Sn a/A-T(1;9)27H/J

000916 B6 x B6C3Sn a/A-T(5;12)31H/J

000602 B6 x B6C3Sn a/A-T(8;16)17H/J

000593 B6 x B6CBCa A^w-J/A-Grid2^Lc T(2;6)7Ca Mitf^Mi-wh/J

000502 B6 x B6CBCa A^w-J/A-Myo5a^flr Gnb5^flr/J

000599 B6 x B6CBCa A^w-J/A-T(5;13)264Ca Kit^W-v/J

002083 B6 x B6EiC3 a/A-T(7;16)235Dn/J

000507 B6 x B6EiC3 a/A-Otc^spf/J

000618 B6 x FSB/GnEi a/a Ctsl^fs/J

000577 B6 x STOCK a Oca2^p Hps5^ru2 Ednrb^s/J

000601 B6 x STOCK a/a T(7;18)50H/J

000592 B6 x STOCK T(2;4)13H a/J

002016 B6(Cg)-A^w-J Eda^Ta-6J Chr Y^B6-Sxr/EiJ

000552 B6-A^w-J-Eda^Ta-6J.Cg-Sxr

001730 B6-A^w-J-Eda^Ta-6J.Cg-Sxr^b Hya^-/J

000841 B6-A^w-J.CBy-Eda^Ta-By/J

001809 B6-A^w-J.Cg-Eda^Ta-6J +/+ Ar^Tfm/J

000600 B6-Gpi1^b x B6CBCa A^w-J/A-T(7;15)9H Gpi1^a/J

000769 B6.C/(HZ18)By-a^t-44J/J

000001 B6.C3 A/a Mgrn1^md/J

000203 B6.C3-A^iy/a/J

001572 B6.C3-a^m-J/J

000017 B6.C3Fe-A^vy/J

000628 B6.CE-A Amy1^b Amy2^b/J

005505 B6.Cg-A^y Slc7a11^sut/LmLlp

000021 B6.Cg-A^y/J

100409 B6129PF1/J-A^w-J/A^w

004200 B6;CBACa A^w-J/A-Npr2^cn-2J/J

000785 B6;D2-a Es1^e/J

000505 B6C3 A^w-J/A-Muted^mu/J

000604 B6C3 a/A-T(10;13)199H +/+ Lyst^bg-J/J or Lyst^bg-2J/J

002807 B6C3Fe a/a-Meox2^fla/J

000224 B6C3Fe a/a-Scyl1^mdf/J

001037 B6C3Fe a/a-Agtpbp1^pcd/J

000221 B6C3Fe a/a-Alx4^lst-J/J

002062 B6C3Fe a/a-Atp7a^Mo-8J/J

001756 B6C3Fe a/a-Cacng2^stg/J

001815 B6C3Fe a/a-Col1a2^oim/J

000231 B6C3Fe a/a-Csf1^op/J

000209 B6C3Fe a/a-Dh/J

000211 B6C3Fe a/a-Dst^dt-J/J

000210 B6C3Fe a/a-Edar^dl-J/J

000207 B6C3Fe a/a-Edaradd^cr/J

000182 B6C3Fe a/a-Eef1a2^wst/J

001278 B6C3Fe a/a-Glra1^spd/J

000241 B6C3Fe a/a-Glrb^spa/J

002875 B6C3Fe a/a-Hoxd13^spdh/J

000304 B6C3Fe a/a-Krt71^Ca Scn8a^med-J/J

000226 B6C3Fe a/a-Large^myd/J

000636 B6C3Fe a/a-Lmx1a^dr-J/J

001280 B6C3Fe a/a-Lse/J

001573 B6C3Fe a/a-Mitf^Mi/J

001035 B6C3Fe a/a-Napa^hyh/J

000181 B6C3Fe a/a-Otog^twt/J

000278 B6C3Fe a/a-Papss2^bm Hps1^ep Hps6^ru/J

000205 B6C3Fe a/a-Papss2^bm/J

002078 B6C3Fe a/a-Pcdh15^av-2J/J

000246 B6C3Fe a/a-Pitpna^vb/J

001430 B6C3Fe a/a-Ptch1^mes/J

000506 B6C3Fe a/a-Qk^qk/J

000235 B6C3Fe a/a-Reln^rl/J

000237 B6C3Fe a/a-Rora^sg/J

000290 B6C3Fe a/a-Sox10^Dom/J

000230 B6C3Fe a/a-Tcirg1^oc/J

003612 B6C3Fe a/a-Trak1^hyrt/J

001512 B6C3Fe a/a-Ttn^mdm/J

001607 B6C3Fe a/a-Unc5c^rcm/J

000005 B6C3Fe a/a-Wc/J

000243 B6C3Fe a/a-Wnt1^sw/J

000248 B6C3Fe a/a-Xpl/J

001750 B6C3Fe a/a-Xs^J/J

000624 B6C3Fe a/a-anx/J

003020 B6C3Fe a/a-dep/J

002018 B6C3Fe a/a-din/J

002339 B6C3Fe a/a-nma/J

000240 B6C3Fe a/a-soc/J

000063 B6C3Fe a/a-sy/J

001055 B6C3Fe a/a-tip/J

000245 B6C3Fe a/a-tn/J

000296 B6C3Fe-a/a Hoxa13^Hd Mcoln3^Va-J/J

000019 B6C3Fe-a/a-Itpr1^opt/J

001022 B6C3FeF1/J a/a

000314 B6CBACa A^w-J/A-Eda^Ta/J-XO

000501 B6CBACa A^w-J/A-Aifm1^Hq/J

001046 B6CBACa A^w-J/A-Grid2^Lc/J

000500 B6CBACa A^w-J/A-Gs/J

002703 B6CBACa A^w-J/A-Hydin^hy3/J

000247 B6CBACa A^w-J/A-Kcnj6^wv/J

000287 B6CBACa A^w-J/A-Plp1^jp Eda^Ta/J

000515 B6CBACa A^w-J/A-Sfn^Er/J

000242 B6CBACa A^w-J/A-spc/J

000288 B6CBACa A^w-J/A-we a Mafb^kr/J

001201 B6CBACaF1/J-A^w-J/A

001752 B6CBCa A^w-J/A-T(7;15)9H/J

000971 B6EiC3 a/A-Och/J

000551 B6EiC3 a/A-Tbx15^de-H/J

006450 B6EiC3 a/A-Vss/J

000557 B6EiC3-+ a/Lnp^Ul A/J

000504 B6EiC3Sn a/A-Cacnb4^lh/J

000553 B6EiC3Sn a/A-Egfr^wa2 Wnt3a^vt/J

000503 B6EiC3Sn a/A-Gy/J

001811 B6EiC3Sn a/A-Otc^spf-ash/J

002343 B6EiC3Sn a/A-Otc^spf/J

000391 B6EiC3Sn a/A-Pax6^Sey-Dey/J

001924 B6EiC3Sn a/A-Ts(17¹⁶)65Dn

001923 B6EiC3Sn a/A-Ts(4¹⁷)2Lws Tim/J

000200 C3FeB6 A/A^w-J-Ank^ank/J

000638 C3FeB6 A/A^w-J-Spnb4^qv-J/J

001203 C3FeB6F1/J A/A^w-J

000225 C3FeLe.B6 a/a-Ptpn6^me/J

008425 C3FeLe.B6-a Trl/J

000198 C3FeLe.B6-a/J

000291 C3FeLe.Cg-a/a Hm Kitl^Sl Krt71^Ca-J/J

001272 C3H/HeSnJ-A^hvy/J

000099 C3HeB/FeJ-A^vy/J

001886 C3HeB/FeJLe a/a-gnd/J

000338 C57BL/6J A^w-J-Eda^Ta-6J/J

000584 C57BL/6J-+ T(1;2)5Ca/a +/J

000258 C57BL/6J-Aⁱ/a/J

000774 C57BL/6J-A^sy/a/J

000569 C57BL/6J-A^w-J-Eda^Ta +/+ Ar^Tfm/J

000051 C57BL/6J-A^w-J/J

000055 C57BL/6J-a^t-33J/J

000070 C57BL/6J-a^td/J

000284 CWD/LeJ

000670 DBA/1J

000671 DBA/2J

001057 HPT/LeJ

000260 JGBF/LeJ

002468 KK.Cg-A^y/J

000283 LT.CAST-A/J

000265 MY/HuLeJ

000308 SSL/LeJ

001759 STOCK A Tyr^c Sha/J

001427 STOCK A^w us/J

000994 STOCK a Myo5a^d Mreg^dsu/J

000064 STOCK a Tyrp1^b Si^si/J

002238 STOCK a Tyrp1^b shmy/J

001433 STOCK a skt/J

000579 STOCK a tp/J

000319 STOCK a us/J

002648 STOCK a/a Cln6^nclf/J

000317 STOCK a/a Egfr^wa2/J

000302 STOCK a/a Mitf^Mi-wh +/+ Itpr1^opt/J

000286 STOCK a/a Myo5a^d fd/+ +/J

000206 STOCK a/a Tyr^c-h/J

001432 STOCK a/a Tyrp1^b sks/Tyrp1^b +/J

000281 STOCK a/a ma ft/ma ft/J

000312 STOCK stb + a/+ Fign^fi a/J

000596 STOCK T(2;11)30H/+ x AEJ-a Gdf5^bp-H/J or A/J-a Gdf5^bp-J/J

000970 STOCK T(2;16)28H A/T(2;16)28H a/J

000590 STOCK T(2;4)1Sn a/J

000594 STOCK T(2;8)26H a/T(2;8)26H a Tyrp1⁺/Tyrp1^b/J

000623 TR/DiEiJ

View Strains carrying other alleles of a (161 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms

Hirschsprung Disease, Susceptibility to, 1; HSCR1 - Models with phenotypic similarity to human disease where etiologies involve orthologs.¹

¹ Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.
Edn3^ls/Edn3⁺
involves: C57BL
pigmentation phenotype

abnormal melanosome morphology (MGI Ref ID J:12724)

reduced number of melanosomes in leg musculature, the Harderian gland, ankle skin and choroid layer, however melanocyte content in hair follicles and membranous labyrinth of the ear is normal

Edn3^ls/Edn3^ls
involves: C57BL
lethality-postnatal

postnatal lethality (MGI Ref ID J:5008)

die between 2 and 3 weeks of age

pigmentation phenotype

abnormal melanocyte morphology (MGI Ref ID J:12724)

impaired expansion and differentiation of epidermal melanoblasts

abnormal melanosome morphology (MGI Ref ID J:12724)

melanosomes are absent in hair, Harderian gland, choroid, leg muscles, and ankle skin and are reduced in the membranous labyrinth of the ear

variable body spotting (MGI Ref ID J:5008)

variable white spotting on the back and the belly is usually all white

skin/coat/nails phenotype

variable body spotting (MGI Ref ID J:5008)

variable white spotting on the back and the belly is usually all white

digestive/alimentary phenotype

megacolon (MGI Ref ID J:12724)

distal four sections of the colon are aganglionic

nervous system phenotype

abnormal enteric ganglia morphology (MGI Ref ID J:5008)

deficiency of ganglion cells of the myenteric plexus of the lower colon

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:
Edn3^ls related

Dermatology Research
Color and White Spotting Defects

Developmental Biology Research
Neural Crest Defects
Neurodevelopmental Defects

Internal/Organ Research
Kidney Defects

Mouse/Human Gene Homologs
Waardenburg-Shah syndrome

Neurobiology Research
Neurodevelopmental Defects
Vestibular and Hearing Defects

Sensorineural Research
Vestibular and Hearing Defects

a^t related

Dermatology Research
Color and White Spotting Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Edn3^ls
Allele Name		lethal spotting
Allele Type		Spontaneous
Common Name(s)		ls;
Strain of Origin		C57BL-a
Gene Symbol and Name		Edn3, endothelin 3
Chromosome		2
Gene Common Name(s)		ET3; MGC15067; MGC61498; RGD1564825; lethal spotting; ls; preproET-3;
General Note		Lethal spotting mutant mice usually die in the third week of life from the megacolon abnormality, which is associated with deficiency of intrinsic ganglion cells in the lower colon. A few homozygotes may survive and breed. Other alleles of this geneserve as models for WAARDENBURG-SHAH SYNDROME. This allele was not studied in this context.
Molecular Note		RT-PCR analysis of this allele revealed a missense C to T change at nucleotide 409 of the coding region which results in a substitution of an Arg-137 residue with a Trp residue. [MGI Ref ID J:22207]

Allele Symbol		a^t
Allele Name		black and tan
Allele Type		Spontaneous
Strain of Origin		English fancy stock
Gene Symbol and Name		a, nonagouti
Chromosome		2
Gene Common Name(s)		AGSW; AGTI; AGTIL; ASP; As; MGC126092; MGC126093; SHEP9; agouti; agouti signal protein; agouti suppressor;
General Note		The black and tan mutation was found by Dunn (J:15011) in a strain obtained from an English fancier. The back is black and the belly cream or yellow. Effects are recessive to A on back but dominant on belly. A/a^t mice resemble A^w/-.Dermal--epidermal recombination experiments have shown that the regional differences in color are determined by gene action in the dermis (J:5672).This allele is recessive to A and A^w on the dorsum and dominant to all agouti alleles on the ventrum except for A^w from which it is indistinguishable (J:78801) .
Molecular Note		This allele comprises a 6 kb insertion containing a retrovirus-like transposable element VL30 and a single 526 bp repeat into the first intron of the agouti gene at the same position as for alleles a and A^w. [MGI Ref ID J:16984]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

Additional References

Baynash AG; Hosoda K; Giaid A; Richardson JA; Emoto N; Hammer RE; Yanagisawa M. 1994. Interaction of endothelin-3 with endothelin-B receptor is essential for development of epidermal melanocytes and enteric neurons. Cell 79(7):1277-85. [PubMed: 8001160] [MGI Ref ID J:22207]

Hosoda K; Hammer RE; Richardson JA; Baynash AG; Cheung JC; Giaid A; Yanagisawa M. 1994. Targeted and natural (piebald-lethal) mutations of endothelin-B receptor gene produce megacolon associated with spotted coat color in mice. Cell 79(7):1267-76. [PubMed: 8001159] [MGI Ref ID J:22206]

Lane PW. 1966. Association of megacolon with two recessive spotting genes in the mouse. J Hered 57(1):29-31. [PubMed: 5917257] [MGI Ref ID J:5008]
Edn3^ls related

Barlow A; de Graaff E; Pachnis V. 2003. Enteric nervous system progenitors are coordinately controlled by the G protein-coupled receptor EDNRB and the receptor tyrosine kinase RET. Neuron 40(5):905-16. [PubMed: 14659090] [MGI Ref ID J:130623]

Baynash AG; Hosoda K; Giaid A; Richardson JA; Emoto N; Hammer RE; Yanagisawa M. 1994. Interaction of endothelin-3 with endothelin-B receptor is essential for development of epidermal melanocytes and enteric neurons. Cell 79(7):1277-85. [PubMed: 8001160] [MGI Ref ID J:22207]

Brito FC; Kos L. 2008. Timeline and distribution of melanocyte precursors in the mouse heart. Pigment Cell Melanoma Res 21(4):464-70. [PubMed: 18444965] [MGI Ref ID J:138178]

Chakder S; McHugh KM; Rattan S. 1997. Inhibitory neurotransmission in lethal spotted mutant mice: a model for Hirschsprung's disease. Gastroenterology 112(5):1575-85. [PubMed: 9136836] [MGI Ref ID J:40527]

Coventry S; Yost C; Palmiter RD; Kapur RP. 1994. Migration of ganglion cell precursors in the ileoceca of normal and lethal spotted embryos, a murine model for Hirschsprung disease. Lab Invest 71(1):82-93. [PubMed: 8041122] [MGI Ref ID J:19496]

Garcia RJ; Ittah A; Mirabal S; Figueroa J; Lopez L; Glick AB; Kos L. 2008. Endothelin 3 induces skin pigmentation in a keratin-driven inducible mouse model. J Invest Dermatol 128(1):131-42. [PubMed: 17611578] [MGI Ref ID J:129906]

Howard MJ; Gershon MD. 1998. Development of LBP110 expression by neural crest-derived enteric precursors: migration and differentiation potential in ls/ls mutant mice. J Neurobiol 35(4):341-54. [PubMed: 9624616] [MGI Ref ID J:48411]

Kapur RP; Yost C; Palmiter RD. 1993. Aggregation chimeras demonstrate that the primary defect responsible for aganglionic megacolon in lethal spotted mice is not neuroblast autonomous. Development 117(3):993-9. [PubMed: 8325247] [MGI Ref ID J:4603]

Lane PW. 1966. Association of megacolon with two recessive spotting genes in the mouse. J Hered 57(1):29-31. [PubMed: 5917257] [MGI Ref ID J:5008]

Liddell RA; Mooers SU; Siegman MJ; McHugh KM. 1993. Altered isoactin gene expression in the affected bowel segments of the lethal spotted mouse. Gastroenterology 105(2):441-8. [PubMed: 8335200] [MGI Ref ID J:14502]

Matsushima Y; Shinkai Y; Kobayashi Y; Sakamoto M; Kunieda T; Tachibana M. 2002. A mouse model of Waardenburg syndrome type 4 with a new spontaneous mutation of the endothelin-B receptor gene. Mamm Genome 13(1):30-5. [PubMed: 11773966] [MGI Ref ID J:76584]

Mayer TC; Maltby EL. 1964. An experimental investigation of pattern development in lethal spotting and belted mouse embryos. Dev Biol 9:269-86. [PubMed: 14138974] [MGI Ref ID J:12724]

Phillips RJS. 1959. New mutants, provisional symbol ls (lethal spotting); my<1>; and agouti-umbrous a<u> Companion Issue to Mouse News Lett #21 :39. [MGI Ref ID J:24725]

Rice J; Doggett B; Sweetser DA; Yanagisawa H; Yanagisawa M; Kapur RP. 2000. Transgenic rescue of aganglionosis and piebaldism in lethal spotted mice. Dev Dyn 217(1):120-32. [PubMed: 10679935] [MGI Ref ID J:59241]

Rothman TP; Chen J; Howard MJ; Costantini F; Schuchardt A; Pachnis V; Gershon MD. 1996. Increased expression of laminin-1 and collagen (IV) subunits in the aganglionic bowel of ls/ls, but not c-ret -/- mice. Dev Biol 178(2):498-513. [PubMed: 8812145] [MGI Ref ID J:35512]

Rothman TP; Gershon MD. 1984. Regionally defective colonization of the terminal bowel by the precursors of enteric neurons in lethal spotted mutant mice. Neuroscience 12(4):1293-311. [PubMed: 6207461] [MGI Ref ID J:13357]

Sandgren K; Larsson LT; Ekelund M; Robinsson D; Ekblad E. 1998. Functional and morphological examination of ganglionic and aganglionic distal gut from the lethal spotted mouse. Eur J Pediatr Surg 8(4):234-9. [PubMed: 9783148] [MGI Ref ID J:113064]

Silvers WK. 1979. The Coat Colors of Mice; A Model for Mammalian Gene Action and Interaction. In: The Coat Colors of Mice. Springer-Verlag, New York. [MGI Ref ID J:78801]

Spencer NJ; Kerrin A; Singer CA; Hennig GW; Gerthoffer WT; McDonnell O. 2008. Identification of capsaicin-sensitive rectal mechanoreceptors activated by rectal distension in mice. Neuroscience 153(2):518-34. [PubMed: 18395992] [MGI Ref ID J:139378]

Ward SM; Gershon MD; Keef K; Bayguinov YR; Nelson C; Sanders KM. 2002. Interstitial cells of Cajal and electrical activity in ganglionic and aganglionic colons of mice. Am J Physiol Gastrointest Liver Physiol 283(2):G445-56. [PubMed: 12121893] [MGI Ref ID J:107855]

a^t related

Bultman SJ; Klebig ML; Michaud EJ; Sweet HO; Davisson MT; Woychik RP. 1994. Molecular analysis of reverse mutations from nonagouti (a) to black-and-tan (a(t)) and white-bellied agouti (Aw) reveals alternative forms of agouti transcripts. Genes Dev 8(4):481-90. [PubMed: 8125260] [MGI Ref ID J:16984]

Candille SI; Raamsdonk CD; Chen C; Kuijper S; Chen-Tsai Y; Russ A; Meijlink F; Barsh GS. 2004. Dorsoventral patterning of the mouse coat by tbx15. PLoS Biol 2(1):E3. [PubMed: 14737183] [MGI Ref ID J:87455]

Dickie MM. 1969. Mutations at the agouti locus in the mouse. J Hered 60(1):20-5. [PubMed: 5798139] [MGI Ref ID J:30922]

Dunn LC. 1928. A Fifth Allelomorph in the Agouti Series of the House Mouse. Proc Natl Acad Sci U S A 14(10):816-9. [PubMed: 16587414] [MGI Ref ID J:15011]

Dunn LC. 1936. Studies on multiple allelomorphic series in the house mouse. I. Description of agouti and albino series of allelomorphs J Genet 33:443-53. [MGI Ref ID J:22600]

Galbraith DB; Wolff GL; Brewer NL. 1980. Hair pigment patterns in different integumental environments of the mouse. Influence of the agouti suppressor (A<s>) mutation on expression of agouti locus alleles. J Hered 71:229-234. [MGI Ref ID J:12033]

Hollander WF. 1973. a<t>/a Mouse News Lett 48:33. [MGI Ref ID J:27528]

Leamy LJ; Hrubant HE. 1971. Effects of alleles at the agouti locus on odontometric traits in the C57BL-6 strain of house mice. Genetics 67(1):87-96. [PubMed: 5556294] [MGI Ref ID J:16571]

Miller MW; Duhl DM; Vrieling H; Cordes SP; Ollmann MM; Winkes BM; Barsh GS. 1993. Cloning of the mouse agouti gene predicts a secreted protein ubiquitously expressed in mice carrying the lethal yellow mutation. Genes Dev 7(3):454-67. [PubMed: 8449404] [MGI Ref ID J:4186]

Poole TW. 1982. The agouti suppressor (As) coat color mutation in mice: developmental effects on the expression of agouti locus alleles. J Exp Zool 220(1):57-64. [PubMed: 7077265] [MGI Ref ID J:6763]

Poole TW; Silvers WK. 1976. The development of regional pigmentation patterns in black and tan (at) mice. J Exp Zool 197(1):115-9. [PubMed: 781177] [MGI Ref ID J:5672]

SILVERS WK. 1958. An experimental approach to action of genes at the agouti locus in the mouse. III. Transplants of newborn Aw-, A-and at-skin to Ay-, Aw-, A-and aa hosts. J Exp Zool 137(1):189-96. [PubMed: 13563791] [MGI Ref ID J:13013]

Sandgren K; Larsson LT; Ekelund M; Robinsson D; Ekblad E. 1998. Functional and morphological examination of ganglionic and aganglionic distal gut from the lethal spotted mouse. Eur J Pediatr Surg 8(4):234-9. [PubMed: 9783148] [MGI Ref ID J:113064]

Silvers WK. 1979. The Coat Colors of Mice; A Model for Mammalian Gene Action and Interaction. In: The Coat Colors of Mice. Springer-Verlag, New York. [MGI Ref ID J:78801]

Summerhayes IC; Franks LM. 1979. Effects of donor age on neoplastic transformation of adult mouse bladder epithelium in vitro. J Natl Cancer Inst 62(4):1017-23. [PubMed: 107359] [MGI Ref ID J:56454]

Vrieling H; Duhl DM; Millar SE; Miller KA; Barsh GS. 1994. Differences in dorsal and ventral pigmentation result from regional expression of the mouse agouti gene. Proc Natl Acad Sci U S A 91(12):5667-71. [PubMed: 8202545] [MGI Ref ID J:18750]

Wolff GL. 1978. Influence of maternal phenotype on metabolic differentiation of agouti locus mutants in the mouse. Genetics 88(3):529-39. [PubMed: 640377] [MGI Ref ID J:5964]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations

Weeks of Age Price* Gender

Cryorecovery Fee $1900.00

*Price(s) in US dollars ($)

Weeks of Age	Price*	Gender
Cryorecovery Fee	$1900.00

Additional Supply Details

Pricing for International shipping destinations

Weeks of Age Price* Gender

Cryorecovery Fee $2470.00

*Price(s) in US dollars ($)

Weeks of Age	Price*	Gender
Cryorecovery Fee	$2470.00

Additional Supply Details

Supply Details

Standard Supply	Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes	Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. *Cryorecovery to establish a Dedicated Supply for greater quantities of mice.* One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX^® Services, Tel: 1-800-422-6423 or 1-207-288-5845. Genomic DNA is available for this strain from the Mouse DNA Resource.
Important Note	This strain is homozygous for a^t and segregating for Edn3^ls.

Control Information

	Control
	Heterozygote from the colony

Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Ordering and Purchasing Information

Purchasing Information
JAX^® Mice Orders
Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. THE LABORATORY EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.

000477	B10.PA-Pldn^pa H3^e a^t/SnJ
000419	B10.UW-H3^b we Pax1^un a^t/SnJ
000065	B6C3Fe a/a-we Pax1^un a^t/J

003301	(C57BL/6J x C3H-Eya1^bor)F1/J
000251	AEJ.Cg-a^e +/a Gdf5^bp-H/J
000202	AEJ/Gn-bd/J
000199	AEJ/GnLeJ
000427	B10.CE-H13^b A^w/(30NX)SnJ
000420	B10.LP-H13^b A^w/Sn
003879	B10;TFLe-a/a T tf/+ tf/J
001538	B6 x B6C3Sn a/A-T(1;9)27H/J
000916	B6 x B6C3Sn a/A-T(5;12)31H/J
000602	B6 x B6C3Sn a/A-T(8;16)17H/J
000593	B6 x B6CBCa A^w-J/A-Grid2^Lc T(2;6)7Ca Mitf^Mi-wh/J
000502	B6 x B6CBCa A^w-J/A-Myo5a^flr Gnb5^flr/J
000599	B6 x B6CBCa A^w-J/A-T(5;13)264Ca Kit^W-v/J
002083	B6 x B6EiC3 a/A-T(7;16)235Dn/J
000507	B6 x B6EiC3 a/A-Otc^spf/J
000618	B6 x FSB/GnEi a/a Ctsl^fs/J
000577	B6 x STOCK a Oca2^p Hps5^ru2 Ednrb^s/J
000601	B6 x STOCK a/a T(7;18)50H/J
000592	B6 x STOCK T(2;4)13H a/J
002016	B6(Cg)-A^w-J Eda^Ta-6J Chr Y^B6-Sxr/EiJ
000552	B6-A^w-J-Eda^Ta-6J.Cg-Sxr
001730	B6-A^w-J-Eda^Ta-6J.Cg-Sxr^b Hya^-/J
000841	B6-A^w-J.CBy-Eda^Ta-By/J
001809	B6-A^w-J.Cg-Eda^Ta-6J +/+ Ar^Tfm/J
000600	B6-Gpi1^b x B6CBCa A^w-J/A-T(7;15)9H Gpi1^a/J
000769	B6.C/(HZ18)By-a^t-44J/J
000001	B6.C3 A/a Mgrn1^md/J
000203	B6.C3-A^iy/a/J
001572	B6.C3-a^m-J/J
000017	B6.C3Fe-A^vy/J
000628	B6.CE-A Amy1^b Amy2^b/J
005505	B6.Cg-A^y Slc7a11^sut/LmLlp
000021	B6.Cg-A^y/J
100409	B6129PF1/J-A^w-J/A^w
004200	B6;CBACa A^w-J/A-Npr2^cn-2J/J
000785	B6;D2-a Es1^e/J
000505	B6C3 A^w-J/A-Muted^mu/J
000604	B6C3 a/A-T(10;13)199H +/+ Lyst^bg-J/J or Lyst^bg-2J/J
002807	B6C3Fe a/a-Meox2^fla/J
000224	B6C3Fe a/a-Scyl1^mdf/J
001037	B6C3Fe a/a-Agtpbp1^pcd/J
000221	B6C3Fe a/a-Alx4^lst-J/J
002062	B6C3Fe a/a-Atp7a^Mo-8J/J
001756	B6C3Fe a/a-Cacng2^stg/J
001815	B6C3Fe a/a-Col1a2^oim/J
000231	B6C3Fe a/a-Csf1^op/J
000209	B6C3Fe a/a-Dh/J
000211	B6C3Fe a/a-Dst^dt-J/J
000210	B6C3Fe a/a-Edar^dl-J/J
000207	B6C3Fe a/a-Edaradd^cr/J
000182	B6C3Fe a/a-Eef1a2^wst/J
001278	B6C3Fe a/a-Glra1^spd/J
000241	B6C3Fe a/a-Glrb^spa/J
002875	B6C3Fe a/a-Hoxd13^spdh/J
000304	B6C3Fe a/a-Krt71^Ca Scn8a^med-J/J
000226	B6C3Fe a/a-Large^myd/J
000636	B6C3Fe a/a-Lmx1a^dr-J/J
001280	B6C3Fe a/a-Lse/J
001573	B6C3Fe a/a-Mitf^Mi/J
001035	B6C3Fe a/a-Napa^hyh/J
000181	B6C3Fe a/a-Otog^twt/J
000278	B6C3Fe a/a-Papss2^bm Hps1^ep Hps6^ru/J
000205	B6C3Fe a/a-Papss2^bm/J
002078	B6C3Fe a/a-Pcdh15^av-2J/J
000246	B6C3Fe a/a-Pitpna^vb/J
001430	B6C3Fe a/a-Ptch1^mes/J
000506	B6C3Fe a/a-Qk^qk/J
000235	B6C3Fe a/a-Reln^rl/J
000237	B6C3Fe a/a-Rora^sg/J
000290	B6C3Fe a/a-Sox10^Dom/J
000230	B6C3Fe a/a-Tcirg1^oc/J
003612	B6C3Fe a/a-Trak1^hyrt/J
001512	B6C3Fe a/a-Ttn^mdm/J
001607	B6C3Fe a/a-Unc5c^rcm/J
000005	B6C3Fe a/a-Wc/J
000243	B6C3Fe a/a-Wnt1^sw/J
000248	B6C3Fe a/a-Xpl/J
001750	B6C3Fe a/a-Xs^J/J
000624	B6C3Fe a/a-anx/J
003020	B6C3Fe a/a-dep/J
002018	B6C3Fe a/a-din/J
002339	B6C3Fe a/a-nma/J
000240	B6C3Fe a/a-soc/J
000063	B6C3Fe a/a-sy/J
001055	B6C3Fe a/a-tip/J
000245	B6C3Fe a/a-tn/J
000296	B6C3Fe-a/a Hoxa13^Hd Mcoln3^Va-J/J
000019	B6C3Fe-a/a-Itpr1^opt/J
001022	B6C3FeF1/J a/a
000314	B6CBACa A^w-J/A-Eda^Ta/J-XO
000501	B6CBACa A^w-J/A-Aifm1^Hq/J
001046	B6CBACa A^w-J/A-Grid2^Lc/J
000500	B6CBACa A^w-J/A-Gs/J
002703	B6CBACa A^w-J/A-Hydin^hy3/J
000247	B6CBACa A^w-J/A-Kcnj6^wv/J
000287	B6CBACa A^w-J/A-Plp1^jp Eda^Ta/J
000515	B6CBACa A^w-J/A-Sfn^Er/J
000242	B6CBACa A^w-J/A-spc/J
000288	B6CBACa A^w-J/A-we a Mafb^kr/J
001201	B6CBACaF1/J-A^w-J/A
001752	B6CBCa A^w-J/A-T(7;15)9H/J
000971	B6EiC3 a/A-Och/J
000551	B6EiC3 a/A-Tbx15^de-H/J
006450	B6EiC3 a/A-Vss/J
000557	B6EiC3-+ a/Lnp^Ul A/J
000504	B6EiC3Sn a/A-Cacnb4^lh/J
000553	B6EiC3Sn a/A-Egfr^wa2 Wnt3a^vt/J
000503	B6EiC3Sn a/A-Gy/J
001811	B6EiC3Sn a/A-Otc^spf-ash/J
002343	B6EiC3Sn a/A-Otc^spf/J
000391	B6EiC3Sn a/A-Pax6^Sey-Dey/J
001924	B6EiC3Sn a/A-Ts(17¹⁶)65Dn
001923	B6EiC3Sn a/A-Ts(4¹⁷)2Lws Tim/J
000200	C3FeB6 A/A^w-J-Ank^ank/J
000638	C3FeB6 A/A^w-J-Spnb4^qv-J/J
001203	C3FeB6F1/J A/A^w-J
000225	C3FeLe.B6 a/a-Ptpn6^me/J
008425	C3FeLe.B6-a Trl/J
000198	C3FeLe.B6-a/J
000291	C3FeLe.Cg-a/a Hm Kitl^Sl Krt71^Ca-J/J
001272	C3H/HeSnJ-A^hvy/J
000099	C3HeB/FeJ-A^vy/J
001886	C3HeB/FeJLe a/a-gnd/J
000338	C57BL/6J A^w-J-Eda^Ta-6J/J
000584	C57BL/6J-+ T(1;2)5Ca/a +/J
000258	C57BL/6J-Aⁱ/a/J
000774	C57BL/6J-A^sy/a/J
000569	C57BL/6J-A^w-J-Eda^Ta +/+ Ar^Tfm/J
000051	C57BL/6J-A^w-J/J
000055	C57BL/6J-a^t-33J/J
000070	C57BL/6J-a^td/J
000284	CWD/LeJ
000670	DBA/1J
000671	DBA/2J
001057	HPT/LeJ
000260	JGBF/LeJ
002468	KK.Cg-A^y/J
000283	LT.CAST-A/J
000265	MY/HuLeJ
000308	SSL/LeJ
001759	STOCK A Tyr^c Sha/J
001427	STOCK A^w us/J
000994	STOCK a Myo5a^d Mreg^dsu/J
000064	STOCK a Tyrp1^b Si^si/J
002238	STOCK a Tyrp1^b shmy/J
001433	STOCK a skt/J
000579	STOCK a tp/J
000319	STOCK a us/J
002648	STOCK a/a Cln6^nclf/J
000317	STOCK a/a Egfr^wa2/J
000302	STOCK a/a Mitf^Mi-wh +/+ Itpr1^opt/J
000286	STOCK a/a Myo5a^d fd/+ +/J
000206	STOCK a/a Tyr^c-h/J
001432	STOCK a/a Tyrp1^b sks/Tyrp1^b +/J
000281	STOCK a/a ma ft/ma ft/J
000312	STOCK stb + a/+ Fign^fi a/J
000596	STOCK T(2;11)30H/+ x AEJ-a Gdf5^bp-H/J or A/J-a Gdf5^bp-J/J
000970	STOCK T(2;16)28H A/T(2;16)28H a/J
000590	STOCK T(2;4)1Sn a/J
000594	STOCK T(2;8)26H a/T(2;8)26H a Tyrp1⁺/Tyrp1^b/J
000623	TR/DiEiJ

Strain Name:

LS/LeJ

Stock Number:

000262

Availability:

Repository-Cryopreserved

Strain Information

Control Information

Related Strains

Phenotype Information

Edn3ls/Edn3+

Edn3ls/Edn3ls

Gene & Allele Information

Genotyping Information

Helpful Links

References

Additional References

Health & Colony Maintenance Information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Additional Supply Details

Additional Supply Details

Supply Details

Control Information

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

Terms of Use

Contact information

Contracts Administration

JAX® Mice & Services Conditions of Use

No Warranty

No Liability

Edn3^ls/Edn3⁺

Edn3^ls/Edn3^ls

JAX^® Mice & Services Conditions of Use