Strain Name: |
MY/HuLeJ |
|---|---|
Stock Number: |
000265 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Frem2; Frem2my; Tyrp1; Tyrp1b; a; |
Product Information
Strain Details
Type Inbred Strain Additional information on Inbred Strains. Type JAX® GEMM® Strain - Spontaneous Mutation Additional information on JAX® GEMM® Strains. Species laboratory mouse H2 Haplotype k Generation F108 Important Note
This strain is homozygous for nonagouti, Tyrp1b and my.Strain Development
The Frem2my mutation was identified in the early 1920s in offspring of irradiated grandparents. What is known of the mice that were irradiated is that they were of "a stock of colored animals that had been inbred for several years and hence was in a fairly homogeneous condition" that they "were obtained from Mrs. Gray, successor to A.E.C. Lathrop" (Bagg and Little,1924), and that the two irradiated founders of this line were both brown (Little and Bagg, 1923). Irradiated female #85 was bred to irradiated male #49, their offspring were sibling mated, and their inbred descendants are line 85. 17 mice in the F3 generation from these irradiated parents showed the most consistent deviant phenotype of Frem2my, eye abnormalities. In 1950 K.P. Hummel at The Jackson Laboratory reported breeding a C57BL/cdJ female to an F71 male from "line 85" which she had received from E.C. MacDowell in 1948 (Hummel, 1950). In 1951 she reported outcrossing an F8 male to a female C3H/Hu (later updated to C3HeB/HuJ by J. Staats, 1968). The F2 from this outcross were then bred to produce a line that was homozygous for a, Tyrpb, and Frem2my (Humel, 1951). In 1968 this homozygous inbred strain, called MY/Hu, was at F31 and has been maintained by sibling breeding since that time. In 1973 it was passed to Priscilla Lane at The Jackson Laboratory where it continued to be maintained by sibling breeding. Isoenzyme profiles in 1983 showed Pep3c and Car2a alleles in MY/HuLe which are distinct from both C3H and C57BR/cdJ profiles, while the other isoenzymes assayed are held in common with either C3H, C57BR/cdJ, or both. MY/HuLe embryos were frozen in 1988 and 1989 by sibling mating at F107 and beyond.
Gene & Allele Details
| Allele Symbol | Frem2my | ||
|---|---|---|---|
| Allele Name | myelencephalic blebs | ||
| Gene Symbol and Name | Frem2, Fras1 related extracellular matrix protein 2 | ||
| Chromosome | 3 | ||
| Gene Common Name(s) | 6030440P17Rik; 8430406N05Rik; DKFZp686J0811; DKFZp781I048; KIAA1074; RGD1566012; RIKEN cDNA 6030440P17 gene; RIKEN cDNA 8430406N05 gene; my; myelencephalic blebs; | ||
| General Note | Found among the descendants of irradiated mice (J:14847). Effects are variable, and penetrance is incomplete. This mutant has been the subject of a large number of investigations reviewed by Gr"uneberg (J:30758). The genetics and development were reinvestigated by Carter (J:221, J:13050) who concluded that the effects of this mutation include: embryonic subepidermal blebs leading to abnormalities of the eyes, skin, and hair, and clubbing of the feet; split sternum, pseudencephaly, acrania, renal agenesis, preaxial polydactyly, and syndactyly of the middle digits; and probably also hydronephrosis, ectopia viscerum, and midcerebral lesions. | ||
| Allele Symbol | Tyrp1b | ||
| Allele Name | brown | ||
| Strain of Origin | C57BL | ||
| Gene Symbol and Name | Tyrp1, tyrosinase-related protein 1 | ||
| Chromosome | 4 | ||
| Gene Common Name(s) | B; CAS2; CATB; GP75; TRP; TRP-1; TRP1; TYRP; Tyrp; b; b-PROTEIN; brown; iris stromal atrophy; isa; tyrosinase-related protein; | ||
| General Note | Tyrp1b, brown, recessive. This type mutant of the brown locus is an old mutation of the mouse fancy. The eumelanin of the hair and eyes is brown rather than black. The pigment granules also appear brown rather than black and are spheroid rather than ovoid in shape (J:12970). The fine structure of the developing pigment granules is fibrillar, like that of wild type mice, but the appearance of the mature granule may be more coarsely granular (J:5346, J:5001, J:5068). The granules incorporate twice as much 14C-tyrosine as normal (J:12173). | ||
| Molecular Note | A G-to-A transition point mutation at position 329 was shown by revertant analysis to be responsible for the mutant phenotype seen in the brown mutant. This mutation is predicted to change a cysteine residue to a tyrosine in the encoded protein. Three other point mutations in the brown sequence were identified, but do not contribute to the mutant phenotype. [J:44435] | ||
| Allele Symbol | a | ||
| Allele Name | nonagouti | ||
Control Information
| Control | ||
|---|---|---|
| Untyped from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Tyrp1b allele
000004 ABP/LeJ 000571 B6.Cg-Whrnwi Tyrp1b/+ +/J 000027 B6.D-Tyrp1b m/J 001045 SI/Col Tyrp1b Dnahc11iv/J 000064 STOCK a Tyrp1b Sisi/J 002238 STOCK a Tyrp1b shmy/J 001432 STOCK a/a Tyrp1b sks/Tyrp1b +/J 000594 STOCK T(2;8)26H a/T(2;8)26H a Tyrp1+/Tyrp1b/J 001101 STOCK T(3;4)5Rk Tyrp1b/J 000274 TSJ/LeJ View Strains carrying Tyrp1b (10 strains)
000068 C57BL/6J-Tyrp1b-J/J 000093 C57BL/6J-Tyrp1b-cJ/J 000671 DBA/2J 003588 LT/SvEi 006252 LT/SvEiJ 002142 STOCK 11R30m/J 000594 STOCK T(2;8)26H a/T(2;8)26H a Tyrp1+/Tyrp1b/J
Research Applications
This mouse can be used to support research in many areas including:Frem2my related
Tyrp1b relatedDevelopmental Biology Research
Craniofacial and Palate Defects
Embryonic Lethality (Homozygous)
Eye Defects
Internal/Organ Defects (kidney)
Skeletal Defects
Internal/Organ Research
Kidney Defects
Sensorineural Research
Cataracts
Eye Defects
Dermatology Research
Color and White Spotting Defects
Mouse/Human Gene Homologs
oculocutaneous albinism type III
References
Additional ReferencesPrice and Supply Information
| Strain Name: | MY/HuLeJ |
| Stock Number: | 000265 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to the Supply Notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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