Strain Name: |
C3FeLe.Cg-a/a Hm KitlSl Krt71Ca-J/J |
|---|---|
Stock Number: |
000291 |
Availability: | Repository-Cryopreserved |
Price and Supply Information | |
General Terms and Conditions |
| Former Name |
C3FeLe.Cg-a/a Hm KitlSl Krt2-6gCa-J/J (Changed: 21-JUL-06
) |
|
C3FeLe.Cg-a/a Hm KitlSl CaJ/J (Changed: 15-DEC-04
) | |
|
C3FeLe.Cg-a/a Hm KitlSl CaJS (Changed: 15-DEC-04
) | |
|
C3FeLe.Cg-a/a-CaJ KitlSl Hm (Changed: 15-DEC-04
) | |
| Genes & Alleles | Hm; Kitl; KitlSl; Krt71; Krt71Ca-J; a; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Species laboratory mouse Strain Description
The multiple steel mutations (KitlSl) behave in a semidominant fashion and cause deficiencies in pigment cells, germ cells, and blood cells paralleling those caused by the Kit locus mutations (dominant spotting alleles). Most of the alleles at steel locus cause severe anemia in utero and death by 15 to 16 days of gestation in homozygous mutant mice. However, compounds of two steel mutants (e.g. KitlSl/KitlSl-d) are viable, black-eyed white, are usually sterile in one or both sexes, and have severe macrocytic anemia. Heterozygous steel mice have a diluted coat color with a small amount of white spotting, are viable and fertile, and may have a slight macrocytic anemia. Primordial germ cells are absent in the nonviable steel homozygotes and severely reduced in steel heterozygotes. Mast cells are virtually absent in skin and other tissues of steel mutant mice. Tumors tend to develop in germ-cell-deficient ovaries with advancing age. This strain is also carrying the caracal-J (Krt71Ca-J) and hammer toe (Hm) mutations.Strain Development
The linkage testing stock Hm, Sl, CaJ was developed from various stocks. It started with a dancer (Dc/+) male mated to a steel (Sl/+) female in 1959. Dancer originated as a spontaneous mutation at the Jackson Laboratory in the C3H/He-Lepob stock at N4 in 1956. Steel (KitlSl) arose spontaneously at the Jackson Laboratory in a C3H inbred strain and was maintained in a non-inbred multiple mutation stock by Dr. M.C. Green. A dancer steel (Dc/+ KitlSl/+) mouse was crossed once to strain C57BL/6J. A dancer steel off spring was crossed to a homozygous caracul (Ca/Ca) mouse from an inbred caracul stock of Dr. G. D. Snell. Caracul was an early mutation that arose in a Swiss stock in the 1930s. The caracul steel cross was sibling mated for 4 generations without dancer and at F4 a caracul steel heterozygote was mated to a twirler (Tw/+) male. Twirler arose spontaneously in the PCS multiple recessive stock at Harwell and was imported to the Jackson Laboratory in 1961. The caracul steel twirler stock (Ca/+ Sl/+ Tw/+) was maintained by sibling and non-sibling matings until 1967 when twirler was replaced with hammer toe (Hm). Hammer toe arose spontaneously at the Jackson Laboratory in a linkage cross in which luxate (lu) was segregating. The three mutations Ca, Sl, and Hm were maintained together and backcrossed to C57BL/6J for 5 generations. In 1969 at N5 sib matings were used and in 1971 caracul (Ca) was replaced with caracul Jackson (CaJ) which had arisen spontaneously in strain C57BL/6J. After several sibling matings with the 3 mutations segregating the Hm/+ Sl/+ CaJ/+ stock was backcrossed to C57BL/6J to N19. In 1977 a B6.Cg-Hm/+ Sl/+ CaJ/+ mouse at N19 was outcrossed to strain C3FeLe.B6-a/J and the strain was then maintained via continued backcrossing to strain C3FeLe.B6-a/J. It reached N76 in 1995. Embryos were generated for cryopreservation in 1989 by mating Hm/+ Sl/+ CaJ/+ mice to a C3FeLe.B6-a/J.
Gene & Allele Details
| Allele Symbol | Hm | ||
|---|---|---|---|
| Allele Name | hammertoe | ||
| Allele Symbol | KitlSl | ||
| Allele Name | steel | ||
| Common Name(s) | MgfSl; Sl; | ||
| Strain of Origin | C3H | ||
| Gene Symbol and Name | Kitl, kit ligand | ||
| Chromosome | 10 | ||
| Gene Common Name(s) | Clo; Con; DKFZp686F2250; Gb; KL-1; Kl1; Kl2; MGF; Mgf; SCF; SF; SHEP7; SLF; Sl; Steel; Steel factor; cloud gray; contrasted; grizzle-belly; mast cell growth factor; steel; stem cell factor; | ||
| General Note | Numerous experiments have demonstrated that precursors of pigment cells and blood cells of homozygotes behave normally when transplanted to an environment of wild-type cells, and that the locus exerts its effect through action of cells in the microenvironment of the apparently affected cells. This steel allele is semidominant. The original steel mutation arose spontaneously in the C3H inbred strain the Oak Ridge National Laboratory. Primordial germ cells are absent in KitlSl/KitlSl and deficient in KitlSl/+. There is no pigment-forming ability in the skin of KitlSl/KitlSl embryos (J:28098). KitlSl derived-fibroblasts are incapable of binding the KIT receptor, as normal fibroblasts can (J:10749). KitlSl is a deletion of the locus (J:10748)(J:6031). | ||
| Molecular Note | By Southern blotting, it was concluded that this allele contains a deletion encompassing most, if not all, of the coding region of the gene. A probe corresponding to nucleotides 6 to 685 of the cDNA failed to hybridize to DNA obtained from embryos homozygous for this allele. PCR analysis with primers for sequences at various distances from the Kit gene narrowed the 5' and 3' deletion endpoints to a 350 and a 380 base-pair region, respectively. Sequencing of the product of PCR using primers designed to span the deletion revealed that it extends through 973,366 base pairs on Chromosome 10 between nucleotide positions 99,177,807 and 100,151,173 (NCBI Map Viewer, Build 36.1), with a 4-base pair insertion joining the deletion endpoints, and contains 6 predicted and 3 known genes. [MGI Ref ID J:10750] [MGI Ref ID J:115283] | ||
| Allele Symbol | Krt71Ca-J | ||
| Allele Name | caracul Jackson | ||
| Strain of Origin | C57BL/6J | ||
| Gene Symbol and Name | Krt71, keratin 71 | ||
| Chromosome | 15 | ||
| Gene Common Name(s) | AA589543; Ca; Cu; K6IRS1; KRT6IRS; KRT6IRS1; Krt2-6g; MGC119390; MGC119391; caracul; curly; expressed sequence AA589543; keratin complex 2, basic, gene 6g; mK6irs; mK6irs1; | ||
| Molecular Note | Sequence analysis of Krt2-6g identified the spontaneous deletion of codon 140, comprised of nucleotides 418, 419, and 420 (CAA). The deleted codon predicted an asparagine in the alpha-helical rod domain. This molecular lesion is the same that has been identified in Krt2-6gCa-Rin, Ca9J, and Ca10J. | ||
| Allele Symbol | a | ||
| Allele Name | nonagouti | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000658 C3HeB/FeJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Related Strains
Strains carrying Hm allele
002338 NFS.Cg-Hm/J View Strains carrying Hm (1 strain)
000124 B6.Cg-KitlSl Krt71Ca/J 000693 WC/ReJ KitlSl/J 100401 WCB6F1/J KitlSl KitlSl-d
000090 129S1/Sv-Oca2+ Tyr+ KitlSl-J/J 002993 B6.Cg-KitlSl-18H/EiJ 000160 B6.D2-KitlSl-d/J 001380 C3Sn.Cg-KitlSl-con/J 003252 C57BL/6J-KitlSl-20J/J 006839 C57BL/6J-KitlSl-22J/J 000979 STOCK KitlSl-16J/J 000161 WB.D2-KitlSl-d/J 100401 WCB6F1/J KitlSl KitlSl-d
000124 B6.Cg-KitlSl Krt71Ca/J 000304 B6C3Fe a/a-Krt71Ca Scn8amed-J/J 001274 BALB/c-Krt71Ca-9J/J 001755 BALB/cBy-Krt71Ca-10J/J
Additional Web Information
Congenic Nomenclature
JAX® NOTES, February 2001; 481. Mgf Gene Name Changes to Kitl.
Research Applications
This mouse can be used to support research in many areas including:Hm related
KitlSl relatedDevelopmental Biology Research
Skeletal Defects
Krt71Ca-J relatedCancer Research
Growth Factors/Receptors/Cytokines
Increased Tumor Incidence (Gonadal Tumors: ovarian and testicular)
Dermatology Research
Color and White Spotting Defects
Developmental Biology Research
Neural Crest Defects
Endocrine Deficiency Research
Bone/Bone Marrow Defects
Gonad Defects
Hypothalamus/Pituitary Defects
Skin Defects
Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Immunodeficiency (Mast Cell Deficiency)
Neurobiology Research
Vestibular and Hearing Defects
Reproductive Biology Research
Developmental Defects Affecting Gonads (germ cell deficient)
Fertility Defects
Gonadal Tumors (ovarian and testicular)
Research Tools
Immunology and Inflammation Research (Mast Cell Deficiency)
Sensorineural Research
Vestibular and Hearing Defects
Dermatology Research
Color and White Spotting Defects
References
Additional ReferencesPrice and Supply Information
| Strain Name: | C3FeLe.Cg-a/a Hm KitlSl Krt71Ca-J/J |
| Stock Number: | 000291 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
International
| Price(s) in US dollars ($) | |||||
|---|---|---|---|---|---|
| Cryorecovery Fee | $2470.00 | ||||
Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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