Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names C57BL/10ScSn-T/J    (Changed: 13-MAR-08 ) Type Congenic; Mutant Strain; Spontaneous Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N18p

Related Strains

Strains carrying   T allele

003879	B10;TFLe-a/a T tf/+ tf/J
001518	B6 x STOCK T tf/th45 tf/J
000350	B6By.Cg-KitW-v MitfMi-wh T/J
001961	C57BL/6JEi x STOCK T T(16;17)43H/+ T(16;17)43H/Ei

View Strains carrying   T     (4 strains)

Strains carrying other alleles of T

004591	B6 x B6Ei.Cg-TWis/EiJ
000953	B6 x BALB/cBy-T4J/J
000567	B6.Cg-T2J +/+ Qkqk/J
001015	B6.Cg-T4Or/J
001054	B6.Cg-TOrl/EiJ
002282	BTBR T+ tf/J
001053	C3Sn.AK-Thp/EiJ
000545	C57BL/6J-T2J/J
001199	C57BL/6J-T5J/J
001814	STOCK Tc/J

View Strains carrying other alleles of T     (10 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

T/T

        Background Not Specified

• lethality-prenatal/perinatal
• embryonic lethality during organogenesis (MGI Ref ID J:13018)
  • die around E11
• embryogenesis phenotype
• abnormal limb bud morphology (MGI Ref ID J:11933)
  • posterior limb buds are absent as the posterior region of the body does not develop
  • anterior limb buds are directed dorsad rather than ventrad
• abnormal placenta development (MGI Ref ID J:11933)
  • results in embryonic death at at E10.75
• abnormal primitive streak formation (MGI Ref ID J:11933)
  • backward growth does not occur preventing organization of the posterior axis and emergence of the allantoic placental stalk
• abnormal somite development (MGI Ref ID J:11933)
  • somites appear normal at early stages but at later stages, they are reduced and by E10, somite tissue is almost absent
• absent notochord (MGI Ref ID J:13018)
  • notochord is absent, except for fragments in early stages
• caudal body truncation (MGI Ref ID J:13018)
  • posterior region of the body is greatly reduced at E10
• decreased embryo size (MGI Ref ID J:13018)
  • structures posterior to the forelimb buds are not formed
• notochord degeneration (MGI Ref ID J:11933)
• growth/size phenotype
• decreased embryo size (MGI Ref ID J:13018)
  • structures posterior to the forelimb buds are not formed
• cardiovascular system phenotype
• abnormal aorta morphology (MGI Ref ID J:13018)
  • one of the two dorsal arotae is obliterated by a diverticulum from the neural tube
• abnormal heart development (MGI Ref ID J:13018)
  • heart development is sometimes retarded
• abnormal pericardium morphology (MGI Ref ID J:13018)
  • commonly, the pericardial cavity is enlarged and is often filled with blood at E10
• hemorrhage (MGI Ref ID J:13018)
  • E10 homozygotes contain numerous blood-filled sinuses in the posterior end
  • pericardial cavity is often filled with blood at E10
• craniofacial phenotype
• abnormal head morphology (MGI Ref ID J:13018)
  • in a few cases, the head region, particularly the mouth, is malformed
• limbs/digits/tail phenotype
• abnormal limb bud morphology (MGI Ref ID J:11933)
  • posterior limb buds are absent as the posterior region of the body does not develop
  • anterior limb buds are directed dorsad rather than ventrad
• short tail (MGI Ref ID J:13018)
• nervous system phenotype
• abnormal nervous system development (MGI Ref ID J:13018)
  • no regular segmental dorsal ganglia are formed in E10 embryos although ganglion tissue is found
• abnormal neural tube morphology/development (MGI Ref ID J:11933)
  • neural tube is slightly irregular at E8.5 and by E9, is markedly irregular in the posterior end; neural tube is more affected in the posterior than anterior end
  • exhibit asymmetry of the neural tube in the mid-region at around E9
  • neural tube sends out branches with secondary lumina and often the branch becomes larger than the primary neural tube
  • in some embryos, the neural tube lumen is ruptured to the outside and is continuous with the amniotic cavity
  • few cases show fusion between the neural tube and the gut in the posterior region
  • abnormal folding and kinking of the neural folds at E8 that culminates in ectopic tubular epithelial structures lying ventral to the developing endogenous neural folds
• kinked neural tube (MGI Ref ID J:59271)
• skin/coat/nails phenotype
• abnormal epidermal layer morphology (MGI Ref ID J:13018)
  • exhibit transient blistering of the dorsal epithelium, first seen at the time of the formation of the first few somites but is completely gone by the time the embryo has attained the second flexure
• bleb (MGI Ref ID J:13018)
  • embryos exhibit small paired or unpaired blebs or small vesicles on either side of the mid-line at E8.5-9

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

T related

Developmental Biology Research
Skeletal Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		T
Allele Name		brachyury
Allele Type		Spontaneous
Common Name(s)		T1;
Strain of Origin		Laboratory stock
Gene Symbol and Name		T, brachyury
Chromosome		17
Gene Common Name(s)		Bra; Low; Lr; MGC104817; T1; TFT; Tl2; Tl3; brachyury-like 2; brachyury-like 3; cou; coupe; low ratio; me75;
Molecular Note		Pulsed-field gel electrophoresis revealed an altered restriction fragment size consistent with a deletion of 160-200kb. [MGI Ref ID J:21263]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Genotyping resources and troubleshooting

References

References

Additional References

T related

Chesley P. 1935. Development of the short-tailed mutant in the house mouse. J Exp Zool 70:429-459.  [MGI Ref ID J:13018]

Dobrovolskaia-Zavadskaia N. 1927. Sur la mortification spontanee de la queue chez la souris nouveau-nee et sur l'existence d'un caractere (facteur) hereditaire "non-viable" C R Seances Soc Biol Fil 97:114-16.  [MGI Ref ID J:14909]

GRUNEBERG H. 1958. Genetical studies on the skeleton of the mouse. XXIII. The development of brachyury and anury. J Embryol Exp Morphol 6(3):424-43. [PubMed: 13575656]  [MGI Ref ID J:15081]

Harrison SM; Houzelstein D; Dunwoodie SL; Beddington RS. 2000. Sp5, a new member of the Sp1 family, is dynamically expressed during development and genetically interacts with brachyury Dev Biol 227(2):358-72. [PubMed: 11071760]  [MGI Ref ID J:65743]

Herrmann BG; Labeit S; Poustka A; King TR; Lehrach H. 1990. Cloning of the T gene required in mesoderm formation in the mouse [see comments] Nature 343(6259):617-22. [PubMed: 2154694]  [MGI Ref ID J:21263]

Kinder SJ; Tsang TE; Ang SL; Behringer RR; Tam PP. 2001. Defects of the body plan of mutant embryos lacking Lim1, Otx2 or Hnf3beta activity. Int J Dev Biol 45(1 Spec No):347-55. [PubMed: 11291865]  [MGI Ref ID J:74124]

King T; Beddington RS; Brown NA. 1998. The role of the brachyury gene in heart development and left-right specification in the mouse. Mech Dev 79(1-2):29-37. [PubMed: 10349618]  [MGI Ref ID J:52218]

Le Saux F; Besson MJ; Maurin Y. 2002. Abnormal postnatal ontogeny of the locus coeruleus in the epileptic mutant mouse quaking. Brain Res Dev Brain Res 136(2):197-205. [PubMed: 12101037]  [MGI Ref ID J:109168]

Nadeau JH. 2001. Modifier genes in mice and humans. Nat Rev Genet 2(3):165-74. [PubMed: 11256068]  [MGI Ref ID J:88013]

Rennebeck GM; Lader E; Chen Q; Bohm RA; Cai ZS; Faust C; Magnuson T; Pease LR; Artzt K. 1995. Is there a Brachyury the Second? Analysis of a transgenic mutation involved in notochord maintenance in mice. Dev Biol 172(1):206-17. [PubMed: 7589801]  [MGI Ref ID J:29666]

Searle AG. 1966. Curtailed, a new dominant T-allele in the house mouse. Genet Res 7(1):86-95. [PubMed: 5906493]  [MGI Ref ID J:5003]

Stott D; Kispert A; Herrmann BG. 1993. Rescue of the tail defect of Brachyury mice. Genes Dev 7(2):197-203. [PubMed: 8436292]  [MGI Ref ID J:3956]

Wittler L; Shin EH; Grote P; Kispert A; Beckers A; Gossler A; Werber M; Herrmann BG. 2007. Expression of Msgn1 in the presomitic mesoderm is controlled by synergism of WNT signalling and Tbx6. EMBO Rep 8(8):784-9. [PubMed: 17668009]  [MGI Ref ID J:129900]

Yamaguchi TP; Takada S; Yoshikawa Y; Wu N; McMahon AP. 1999. T (Brachyury) is a direct target of Wnt3a during paraxial mesoderm specification. Genes Dev 13(24):3185-90. [PubMed: 10617567]  [MGI Ref ID J:59271]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location). Genomic DNA is available for this strain from the Mouse DNA Resource.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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