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Former Names C57BL/10ScSn-T/J (Changed: 13-MAR-08 ) Type Congenic; Mutant Strain; Spontaneous Mutation; Species laboratory mouse Generation N18p
Strains carrying T allele
003879 B10;TFLe-a/a T tf/+ tf/J 001518 B6 x STOCK T tf/th45 tf/J 000350 B6By.Cg-KitW-v MitfMi-wh T/J 001961 C57BL/6JEi x STOCK T T(16;17)43H/+ T(16;17)43H/Ei View Strains carrying T (4 strains)
Strains carrying other alleles of T
004591 B6 x B6Ei.Cg-TWis/EiJ 000953 B6 x BALB/cBy-T4J/J 000567 B6.Cg-T2J +/+ Qkqk/J 001015 B6.Cg-T4Or/J 001054 B6.Cg-TOrl/EiJ 002282 BTBR T+ tf/J 001053 C3Sn.AK-Thp/EiJ 000545 C57BL/6J-T2J/J 001199 C57BL/6J-T5J/J 001814 STOCK Tc/J View Strains carrying other alleles of T (10 strains)
Congenic Nomenclature
Mammalian Phenotype Terms assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
T/T
Background Not Specified
- lethality-prenatal/perinatal
- embryonic lethality during organogenesis (MGI Ref ID J:13018)
- die around E11
- embryogenesis phenotype
- abnormal limb bud morphology (MGI Ref ID J:11933)
- posterior limb buds are absent as the posterior region of the body does not develop
- anterior limb buds are directed dorsad rather than ventrad
- abnormal placenta development (MGI Ref ID J:11933)
- results in embryonic death at at E10.75
- abnormal primitive streak formation (MGI Ref ID J:11933)
- backward growth does not occur preventing organization of the posterior axis and emergence of the allantoic placental stalk
- abnormal somite development (MGI Ref ID J:11933)
- somites appear normal at early stages but at later stages, they are reduced and by E10, somite tissue is almost absent
- absent notochord (MGI Ref ID J:13018)
- notochord is absent, except for fragments in early stages
- caudal body truncation (MGI Ref ID J:13018)
- posterior region of the body is greatly reduced at E10
- notochord degeneration (MGI Ref ID J:11933)
- reduced embryo size (MGI Ref ID J:13018)
- structures posterior to the forelimb buds are not formed
- growth/size phenotype
- reduced embryo size (MGI Ref ID J:13018)
- structures posterior to the forelimb buds are not formed
- cardiovascular system phenotype
- abnormal aorta morphology (MGI Ref ID J:13018)
- one of the two dorsal arotae is obliterated by a diverticulum from the neural tube
- abnormal heart development (MGI Ref ID J:13018)
- heart development is sometimes retarded
- abnormal pericardium morphology (MGI Ref ID J:13018)
- commonly, the pericardial cavity is enlarged and is often filled with blood at E10
- hemorrhage (MGI Ref ID J:13018)
- E10 homozygotes contain numerous blood-filled sinuses in the posterior end
- pericardial cavity is often filled with blood at E10
- craniofacial phenotype
- abnormal head morphology (MGI Ref ID J:13018)
- in a few cases, the head region, particularly the mouth, is malformed
- limbs/digits/tail phenotype
- abnormal limb bud morphology (MGI Ref ID J:11933)
- posterior limb buds are absent as the posterior region of the body does not develop
- anterior limb buds are directed dorsad rather than ventrad
- short tail (MGI Ref ID J:13018)
- nervous system phenotype
- abnormal nervous system development (MGI Ref ID J:13018)
- no regular segmental dorsal ganglia are formed in E10 embryos although ganglion tissue is found
- abnormal neural tube morphology/development (MGI Ref ID J:11933)
- neural tube is slightly irregular at E8.5 and by E9, is markedly irregular in the posterior end; neural tube is more affected in the posterior than anterior end
- exhibit asymmetry of the neural tube in the mid-region at around E9
- neural tube sends out branches with secondary lumina and often the branch becomes larger than the primary neural tube
- in some embryos, the neural tube lumen is ruptured to the outside and is continuous with the amniotic cavity
- few cases show fusion between the neural tube and the gut in the posterior region
- abnormal folding and kinking of the neural folds at E8 that culminates in ectopic tubular epithelial structures lying ventral to the developing endogenous neural folds
- kinked neural tube (MGI Ref ID J:59271)
- skin/coat/nails phenotype
- abnormal epidermal layer morphology (MGI Ref ID J:13018)
- exhibit transient blistering of the dorsal epithelium, first seen at the time of the formation of the first few somites but is completely gone by the time the embryo has attained the second flexure
- bleb (MGI Ref ID J:13018)
- embryos exhibit small paired or unpaired blebs or small vesicles on either side of the mid-line at E8.5-9
Research Applications
This mouse can be used to support research in many areas including:T related
Developmental Biology Research
Skeletal Defects
| Allele Symbol | T | ||
|---|---|---|---|
| Allele Name | brachyury | ||
| Strain of Origin | Laboratory stock | ||
| Molecular Note | Pulsed-field gel electrophoresis revealed an altered restriction fragment size consistent with a deletion of 160-200kb. [MGI Ref ID J:21263] | ||
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Optimizing PCR Protocols
T related
Chesley P. 1935. Development of the short-tailed mutant in the house mouse. J Exp Zool 70:429-459. [MGI Ref ID J:13018]Dobrovolskaia-Zavadskaia N. 1927. Sur la mortification spontanee de la queue chez la souris nouveau-nee et sur l'existence d'un caractere (facteur) hereditaire "non-viable" C R Seances Soc Biol Fil 97:114-16. [MGI Ref ID J:14909]
GRUNEBERG H. 1958. Genetical studies on the skeleton of the mouse. XXIII. The development of brachyury and anury. J Embryol Exp Morphol 6(3):424-43. [PubMed: 13575656] [MGI Ref ID J:15081]
Harrison SM; Houzelstein D; Dunwoodie SL; Beddington RS. 2000. Sp5, a new member of the Sp1 family, is dynamically expressed during development and genetically interacts with brachyury Dev Biol 227(2):358-72. [PubMed: 11071760] [MGI Ref ID J:65743]
Herrmann BG; Labeit S; Poustka A; King TR; Lehrach H. 1990. Cloning of the T gene required in mesoderm formation in the mouse [see comments] Nature 343(6259):617-22. [PubMed: 2154694] [MGI Ref ID J:21263]
Kinder SJ; Tsang TE; Ang SL; Behringer RR; Tam PP. 2001. Defects of the body plan of mutant embryos lacking Lim1, Otx2 or Hnf3beta activity. Int J Dev Biol 45(1 Spec No):347-55. [PubMed: 11291865] [MGI Ref ID J:74124]
King T; Beddington RS; Brown NA. 1998. The role of the brachyury gene in heart development and left-right specification in the mouse. Mech Dev 79(1-2):29-37. [PubMed: 10349618] [MGI Ref ID J:52218]
Le Saux F; Besson MJ; Maurin Y. 2002. Abnormal postnatal ontogeny of the locus coeruleus in the epileptic mutant mouse quaking. Brain Res Dev Brain Res 136(2):197-205. [PubMed: 12101037] [MGI Ref ID J:109168]
Searle AG. 1966. Curtailed, a new dominant T-allele in the house mouse. Genet Res 7(1):86-95. [PubMed: 5906493] [MGI Ref ID J:5003]
Stott D; Kispert A; Herrmann BG. 1993. Rescue of the tail defect of Brachyury mice. Genes Dev 7(2):197-203. [PubMed: 8436292] [MGI Ref ID J:3956]
Wittler L; Shin EH; Grote P; Kispert A; Beckers A; Gossler A; Werber M; Herrmann BG. 2007. Expression of Msgn1 in the presomitic mesoderm is controlled by synergism of WNT signalling and Tbx6. EMBO Rep 8(8):784-9. [PubMed: 17668009] [MGI Ref ID J:129900]
Yamaguchi TP; Takada S; Yoshikawa Y; Wu N; McMahon AP. 1999. T (Brachyury) is a direct target of Wnt3a during paraxial mesoderm specification. Genes Dev 13(24):3185-90. [PubMed: 10617567] [MGI Ref ID J:59271]
Currently there no information available for this strain. This may be due to the supply level of this strain.
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
|
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
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