Strain Name:

B6.C3-Spta1sph/BrkJ

Stock Number:

000450

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.C3-Spna1sph/BrkJ    (Changed: 05-DEC-12 )
B6.C3-Spna1sph/J    (Changed: 18-AUG-10 )
Type Congenic; Mutant Strain;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationN42
Generation Definitions

Description
Homozygotes have spherocytic hemolytic anemia and neonatal jaundice. Erythrocytes have diminished biconcavity, a smaller than normal diameter, increased density, an appearance of exovesiculation, and a short lifespan. At birth homozygotes are very pale then become yellow during the first few hours of life and die generally within a day of being born. Although it is rare for a spherocytosis homozygote to survive beyond one day of birth on the C57BL/6J or WB/Re (Stock No. 000454) congenic background, homozygotes on the (C57BL/6J x WB/Re)F1 background are much more robust and the majority survive to adulthood. Those that make it to wean age are expected to have a reasonable lifespan, sometimes living as long as a year. Thus, most studies of this mutation in the adult have been done on the (C57BL/6J x WB/Re)F1 background. These homozygotes display diminished hematocrit, decreased red blood cell count, very elevated red blood cell protoporphyrin levels, increased erythropoiesis, reticulocytosis, hyperbilirubinaemia, cardiomegaly, hepatomegaly, and severe splenomegaly predominantly due to increased red pulp. Large areas of splenic necrosis and fibrosis develop. Thrombi can be found in the atrioventricular valves or within the atria of adults and infarcts have been observed in the myocardium and in the cerebrum, hippocampus, and cerebellum. Iron accumulates in the liver and kidneys, blood urea nitrogen levels become high, and hydronephrosis and membranoproliferative glomerulonephritis are found. By 2 to 3 months of age the urine becomes green due to hemosiderin and ferritin laden renal tubular cells and the urine later becomes red. Kaysser et al. posited that the likely cause of death in adult homozygotes is renal failure.

Development
The spherocytosis mutation arose spontaneously in 1959 at the University of British Columbia Central Animal Depot in the progeny of an unpedigreed stock from Rockland Farms, reported to be C3H. This mutation was imported into The Jackson Laboratory before 1970 and backcrossed onto the C57BL/6J and WB/Re backgrounds by Dr. Elizabeth Russell and Dr. Seldon Bernstein. In 1970 this congenic was at backcross generation N2, in 1976 it reached N9, in 1986 it reached N21 and was subsequently passed into the care of Dr. Jane Barker. In the year 2000 embryos were generated for cryopreservation from C57BL/6J females bred to heterozygous males at generation N42.

Related Strains

Strains carrying   Spta1sph allele
000454   WB.C3-Spta1sph/BrkJ
View Strains carrying   Spta1sph     (1 strain)

Strains carrying other alleles of Spta1
003815   B6.CcS3-Spta1sph-Dem/BrkJ
006210   B6.Cg-Spta1ihj/LlpJ
001185   B6.Cg-Spta1sph-2Bc/BrkJ
000446   B6.D1-Spta1sph-ha/BrkJ
007875   B6.NOD(Cg)-Spta1sph-3J/LlpJ
003816   WB.CcS3-Spta1sph-Dem/BrkJ
001184   WB.Cg-Spta1sph-2Bc/BrkJ
000451   WB.D1-Spta1sph-ha/BrkJ
View Strains carrying other alleles of Spta1     (8 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).
Spherocytosis, Type 3; SPH3
- Model with phenotypic similarity to human disease where etiologies are distinct. Human genes are associated with this disease. Orthologs of these genes do not appear in the mouse genotype(s).
Spherocytosis, Type 1; SPH1
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Elliptocytosis 2; EL2   (SPTA1)
Pyropoikilocytosis, Hereditary; HPP   (SPTA1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Spta1sph/Spta1sph

        B6.C3-Spta1sph/BrkJ
  • mortality/aging
  • partial neonatal lethality   (MGI Ref ID J:162758)
  • partial postnatal lethality
    • on the inbred WB/Re or C57BL/6J backgrounds homozygotes rarely live to weaning, although a few may, so this mutation is best studied on the F1 hybrid background on which the majority of homozygotes survive to adulthood and some can live to a year or more   (MGI Ref ID J:162758)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Spta1sph/Spta1+

        either: (B6.C3-Spta1sph x WB.C3-Spta1sph)F1 or (WB.C3-Spta1sph x B6.C3-Spta1sph)F1
  • hematopoietic system phenotype
  • increased erythrocyte protoporphyrin level
    • red blood cells show a higher concentration of protoporphyrin than wild-type   (MGI Ref ID J:5985)
  • homeostasis/metabolism phenotype
  • increased erythrocyte protoporphyrin level
    • red blood cells show a higher concentration of protoporphyrin than wild-type   (MGI Ref ID J:5985)

Spta1sph/Spta1sph

        involves: C3H
  • mortality/aging
  • complete neonatal lethality
    • none survive for more than 24 hours   (MGI Ref ID J:12276)
  • hematopoietic system phenotype
  • abnormal reticulocyte morphology
    • reticulocytes do not accumulate alpha spectrin in their membranes and do not synthesize detectable amounts of alpha spectrin   (MGI Ref ID J:7501)
  • decreased hematocrit   (MGI Ref ID J:12276)
  • decreased hemoglobin content   (MGI Ref ID J:12276)
  • hemolytic anemia   (MGI Ref ID J:12276)
  • poikilocytosis   (MGI Ref ID J:12276)
  • spherocytosis   (MGI Ref ID J:12276)
  • cardiovascular system phenotype
  • enlarged liver sinusoidal spaces   (MGI Ref ID J:12276)
  • liver vascular congestion
    • livers show considerable congestion   (MGI Ref ID J:12276)
  • homeostasis/metabolism phenotype
  • increased circulating bilirubin level   (MGI Ref ID J:12276)
  • liver/biliary system phenotype
  • abnormal liver morphology
    • livers are much darker than in control littermates   (MGI Ref ID J:12276)
    • enlarged liver sinusoidal spaces   (MGI Ref ID J:12276)
    • enlarged liver   (MGI Ref ID J:12276)
    • liver vascular congestion
      • livers show considerable congestion   (MGI Ref ID J:12276)
  • jaundice
    • gradually acquire a yellow color during the first few hours of life   (MGI Ref ID J:12276)
  • integument phenotype
  • pallor
    • extremely pale at birth   (MGI Ref ID J:12276)

Spta1sph/Spta1sph

        either: (B6.C3-Spta1sph x WB.C3-Spta1sph)F1 or (WB.C3-Spta1sph x B6.C3-Spta1sph)F1
  • hematopoietic system phenotype
  • abnormal bone marrow cell number
    • granuloid:erythroid ratio is 1:3 compared to 3:1 in controls or 1:1 in bled controls   (MGI Ref ID J:6695)
    • CFU-E concentrations in bone marrow are significantly increased over those in wild-type, while BFU-E concentrations are halved   (MGI Ref ID J:6695)
    • increased bone marrow cell number
      • bone marrow is hypercellular with very high erythrocyte numbers   (MGI Ref ID J:6695)
  • abnormal erythrocyte physiology
    • erythrocyte intracellular sodium levels are three times higher than normal, erythrocyte intracellular potassium levels are lower than normal, and ouabain induced cation flux rates in erythrocytes are approximately three times higher than normal   (MGI Ref ID J:30033)
  • abnormal erythropoiesis
    • accelerated erythropoiesis that is associated with distinctive, dark branched cells that make up large portions of the spleen and bone marrow stroma   (MGI Ref ID J:6695)
    • decreased hematocrit
      • hematocrit average of 15% compared to 45% in controls   (MGI Ref ID J:6695)
    • increased erythrocyte protoporphyrin level
      • red blood cell protoporphyrin levels are about 10 times higher than in controls   (MGI Ref ID J:5985)
      • scanning electron microscopy of erythrocytes shows that they have an abnormal shape with disappearance of biconcavity, smaller diameter, and the appearance of exovesiculation   (MGI Ref ID J:111131)
      • Percoll-stractan gradients show a considerable increase in erythrocyte density   (MGI Ref ID J:111131)
    • increased erythroid progenitor cell number
      • bone marrow contains higher numbers of erythroblasts, proerythroblasts and basophilic erythroblasts   (MGI Ref ID J:6695)
    • macrocytosis   (MGI Ref ID J:6695)
    • microcytosis   (MGI Ref ID J:6695)
    • reticulocytosis   (MGI Ref ID J:6695)
    • spherocytosis   (MGI Ref ID J:6695)
  • abnormal spleen morphology
    • the spleen is extremely erythropoietic and eryhtroclastic, with sheets of erythroblasts associated with dark branching cells alternating with areas filled with macrophages and red cell debris   (MGI Ref ID J:6695)
    • enlarged spleen   (MGI Ref ID J:6695)
  • hemolytic anemia   (MGI Ref ID J:6695)
  • immune system phenotype
  • abnormal spleen morphology
    • the spleen is extremely erythropoietic and eryhtroclastic, with sheets of erythroblasts associated with dark branching cells alternating with areas filled with macrophages and red cell debris   (MGI Ref ID J:6695)
    • enlarged spleen   (MGI Ref ID J:6695)
  • decreased susceptibility to parasitic infection
    • homozygotes are resistant to the malarial parasites, Plasmodium chabaudi adami and Plasmodium berghei   (MGI Ref ID J:111131)
  • homeostasis/metabolism phenotype
  • increased erythrocyte protoporphyrin level
    • red blood cell protoporphyrin levels are about 10 times higher than in controls   (MGI Ref ID J:5985)
    • scanning electron microscopy of erythrocytes shows that they have an abnormal shape with disappearance of biconcavity, smaller diameter, and the appearance of exovesiculation   (MGI Ref ID J:111131)
    • Percoll-stractan gradients show a considerable increase in erythrocyte density   (MGI Ref ID J:111131)

Spta1sph/Spta1sph

        (WB.C3-Spta1sph x B6.C3-Spta1sph)F1
  • mortality/aging
  • partial postnatal lethality
    • approximately 25% of homozygotes die postnatally   (MGI Ref ID J:44313)
    • 28% die before weaning   (MGI Ref ID J:162532)
  • premature death
    • while approximately 75% of homozygotes on this mixed background survive to adulthood, the average lifespan is 5.75 months while approximately 75% of homozygotes survive to adulthood, the average lifespan is 5.75 months   (MGI Ref ID J:44313)
  • hematopoietic system phenotype
  • abnormal erythrocyte morphology
    • cell surface expression of phosphatidylserine is higher than in wild-type controls, with 13.1% of erythrocytes straining positive for phosphatidylserine versus 1.6% in wild-type controls   (MGI Ref ID J:62355)
    • anisopoikilocytosis   (MGI Ref ID J:162532)
    • decreased cellular hemoglobin content
      • decreased to 16.9% compared to 29.8% in controls   (MGI Ref ID J:44313)
    • decreased erythrocyte cell number
      • adults have very low red blood cell count, 3,740,000/ul instead of 9,150,000/ul   (MGI Ref ID J:44313)
      • low mean erythrocyte cell number
        • less than half of normal numbers   (MGI Ref ID J:162532)
    • decreased hematocrit
      • hematocrit of adults is much lower than normal, 22.5% instead of 45% in controls   (MGI Ref ID J:44313)
      • mean hematocrit is reduced to 24.9% from 50.4% in wild-type controls   (MGI Ref ID J:162532)
    • decreased hemoglobin content
      • greatly reduced hemoglobin of 3.7 g/dL compared to 13.5 g/dL in controls   (MGI Ref ID J:44313)
      • mean hemoglobin content is reduced to 5.35 g/dl from 15.64 g/dl in wild-type controls   (MGI Ref ID J:162532)
    • increased mean corpuscular volume
      • mean MCV raised from 48.1 in wild-type controls to 59.7   (MGI Ref ID J:162532)
    • increased nucleated erythrocyte cell number
      • more than 4 times normal levels   (MGI Ref ID J:162532)
    • microcytosis
      • the percentage of erythroid cells that are microcytes is much larger than in wild-type controls   (MGI Ref ID J:62355)
    • poikilocytosis   (MGI Ref ID J:162532)
      • schistocytosis   (MGI Ref ID J:162532)
  • abnormal erythrocyte physiology
    • sodium content of erythrocytes is elevated to 64.2 mEq/l from 12.4 mEq/l in wild-type controls, and the membrane cholesterol and phospholipid content is reduced   (MGI Ref ID J:162532)
    • hemolysis   (MGI Ref ID J:162532)
      • abnormal erythrocyte osmotic lysis
        • the osmotic fragility curve for erythrocytes shows broader sensitivity to osmotic lysis, with a subpopulation more sensitive and a suppopulation less sensitive   (MGI Ref ID J:162532)
    • increased erythrocyte clearance
      • average erythrocyte lifespan of approximately 1 day   (MGI Ref ID J:162532)
  • enlarged spleen   (MGI Ref ID J:162532)
    • severe splenomegaly with areas of necrosis and fibrosis develops in adults with most of the splenic mass made of red pulp   (MGI Ref ID J:44313)
  • extramedullary hematopoiesis   (MGI Ref ID J:162532)
  • hemolytic anemia   (MGI Ref ID J:162532)
  • reticulocytosis
    • mean percent of reticulocytes is substantially increased from 3.16% in wild-type controls to 91.2%   (MGI Ref ID J:162532)
  • immune system phenotype
  • enlarged spleen   (MGI Ref ID J:162532)
    • severe splenomegaly with areas of necrosis and fibrosis develops in adults with most of the splenic mass made of red pulp   (MGI Ref ID J:44313)
  • glomerulonephritis   (MGI Ref ID J:44313)
  • tubular nephritis   (MGI Ref ID J:44313)
  • homeostasis/metabolism phenotype
  • increased circulating bilirubin level   (MGI Ref ID J:162532)
  • increased kidney iron level
    • extraordinary amounts of iron accumulate in the adult kidney, particularly within the proximal convoluted tubules, leading to green colored urine by 2 to 3 months of age and progressing to hydronephrosis and possible renal failure   (MGI Ref ID J:44313)
  • increased liver iron level
    • significant iron stores are found in hepatic cells of the adult, but not in the hematopoietic regions of the liver or in the Kupffer cells   (MGI Ref ID J:44313)
  • thrombosis   (MGI Ref ID J:44313)
    • 100% of homozygous adults display cardiac thrombi   (MGI Ref ID J:62355)
    • atrial thrombosis
      • in surviving adults large thrombi are found within the heart mainly in the mitral or left atrioventricular valve   (MGI Ref ID J:44313)
  • renal/urinary system phenotype
  • glomerulonephritis   (MGI Ref ID J:44313)
  • increased kidney iron level
    • extraordinary amounts of iron accumulate in the adult kidney, particularly within the proximal convoluted tubules, leading to green colored urine by 2 to 3 months of age and progressing to hydronephrosis and possible renal failure   (MGI Ref ID J:44313)
  • renal tubular necrosis   (MGI Ref ID J:44313)
  • tubular nephritis   (MGI Ref ID J:44313)
  • liver/biliary system phenotype
  • enlarged liver   (MGI Ref ID J:162532)
  • focal hepatic necrosis   (MGI Ref ID J:44313)
  • increased liver iron level
    • significant iron stores are found in hepatic cells of the adult, but not in the hematopoietic regions of the liver or in the Kupffer cells   (MGI Ref ID J:44313)
  • jaundice
    • although not jaundiced at birth, homozygotes develop severe jaundice within hours of being born   (MGI Ref ID J:162532)
  • cardiovascular system phenotype
  • enlarged heart
    • cardiac chambers of surviving adults are dilated   (MGI Ref ID J:44313)
    • cardiac hypertrophy   (MGI Ref ID J:162532)
  • myocardial necrosis
    • infarcts are found in the myocardium, ventricular or ventricular septal regions, and atrial walls of surviving adult homozygotes   (MGI Ref ID J:44313)
  • nervous system phenotype
  • abnormal brain morphology
    • infarctions are found in adult cerebrum, hippocampus, and cerebellum, are generally unilateral, and microthrombi are sometimes found close to the infarction   (MGI Ref ID J:44313)
  • growth/size/body phenotype
  • decreased body size   (MGI Ref ID J:162532)
  • reproductive system phenotype
  • infertility   (MGI Ref ID J:162532)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Spta1sph related

Cardiovascular Research
Vascular Defects
      Thrombosis

Hematological Research
Anemia, Iron Deficiency and Transport Defects
      hemolytic
Hematopoietic Defects
Jaundice

Internal/Organ Research
Kidney Defects
Spleen Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Spta1sph
Allele Name spherocytosis
Allele Type Spontaneous
Common Name(s) sph;
Strain of OriginC3H
Gene Symbol and Name Spta1, spectrin alpha, erythrocytic 1
Chromosome 1
Gene Common Name(s) AF093576; AI451697; EL2; HPP; HS3; SPH3; SPTA; Spna-1; Spna1; erythroid; expressed sequence AF093576; expressed sequence AI451697; ha; hemolytic anemia; iasi hereditary jaundice; ihj; spectrin alpha 1; sph; spherocytosis;
General Note This original spherocytosis mutation arose in an unpedigreed C3H stock.
Molecular Note The mutation in the sph mouse was identified as a single base pair deletion in exon 11 that causes a frame shift and premature stop codon. [MGI Ref ID J:80999]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Spta1sph related

Bernstein SE. 1969. Hereditary disorders of the rodent erythron. In: Genetics in Laboratory Animal Medicine. Natl Acad Sci Publ, Washington, DC.  [MGI Ref ID J:30699]

Bernstein SE. 1980. Inherited hemolytic disease in mice: a review and update. Lab Anim Sci 30(2 Pt 1):197-205. [PubMed: 6763106]  [MGI Ref ID J:162532]

Birkenmeier C. 2010. Genetic background effects on spherocytosis Personal Communication :.  [MGI Ref ID J:162758]

Birkenmeier CS; McFarland-Starr EC; Barker JE. 1988. Chromosomal location of three spectrin genes: relationship to the inherited hemolytic anemias of mouse and man. Proc Natl Acad Sci U S A 85(21):8121-5. [PubMed: 3186715]  [MGI Ref ID J:9457]

Bodine DM 4th; Birkenmeier CS; Barker JE. 1984. Spectrin deficient inherited hemolytic anemias in the mouse: characterization by spectrin synthesis and mRNA activity in reticulocytes. Cell 37(3):721-9. [PubMed: 6234993]  [MGI Ref ID J:7501]

Brookoff D; Maggio-Price L; Bernstein S; Weiss L. 1982. Erythropoiesis in ha/ha and sph/sph mice, mutants which produce spectrin-deficient erythrocytes. Blood 59(3):646-51. [PubMed: 7059672]  [MGI Ref ID J:6695]

Campanella ME; Chu H; Wandersee NJ; Peters LL; Mohandas N; Gilligan DM; Low PS. 2008. Characterization of glycolytic enzyme interactions with murine erythrocyte membranes in wild-type and membrane protein knockout mice. Blood 112(9):3900-6. [PubMed: 18698006]  [MGI Ref ID J:142120]

Dahl SC; Geib RW; Fox MT; Edidin M; Branton D. 1994. Rapid capping in alpha-spectrin-deficient MEL cells from mice afflicted with hereditary hemolytic anemia. J Cell Biol 125(5):1057-65. [PubMed: 8195289]  [MGI Ref ID J:19025]

Frei AC; Guo Y; Jones DW; Pritchard KA Jr; Fagan KA; Hogg N; Wandersee NJ. 2008. Vascular dysfunction in a murine model of severe hemolysis. Blood 112(2):398-405. [PubMed: 18477769]  [MGI Ref ID J:138597]

Hanson MS; Xu H; Flewelen TC; Holzhauer SL; Retherford D; Jones DW; Frei AC; Pritchard KA Jr; Hillery CA; Hogg N; Wandersee NJ. 2013. A novel hemoglobin-binding peptide reduces cell-free hemoglobin in murine hemolytic anemia. Am J Physiol Heart Circ Physiol 304(2):H328-36. [PubMed: 23125208]  [MGI Ref ID J:192837]

JOE M; TEASDALE JM; MILLER JR. 1962. A new mutation (sph) causing neonatal jaundice in the house mouse. Can J Genet Cytol 4:219-25. [PubMed: 14451913]  [MGI Ref ID J:12276]

Joiner CH; Franco RS; Jiang M; Franco MS; Barker JE; Lux SE. 1995. Increased cation permeability in mutant mouse red blood cells with defective membrane skeletons. Blood 86(11):4307-14. [PubMed: 7492791]  [MGI Ref ID J:30033]

Kaysser TM; Wandersee NJ; Bronson RT; Barker JE. 1997. Thrombosis and secondary hemochromatosis play major roles in the pathogenesis of jaundiced and spherocytic mice, murine models for hereditary spherocytosis. Blood 90(11):4610-9. [PubMed: 9373273]  [MGI Ref ID J:44313]

Peters LL; Barker JE. 2001. Spontaneous and targeted mutations in erthrocyte membrane skeleton genes: mouse models of heredity spherocytosis. In: Hematopoiesis A Developmental Approach. Oxford University Press.  [MGI Ref ID J:88022]

Robledo RF; Lambert AJ; Birkenmeier CS; Cirlan MV; Cirlan AF; Campagna DR; Lux SE; Peters LL. 2010. Analysis of novel sph (spherocytosis) alleles in mice reveals allele-specific loss of band 3 and adducin in {alpha}-spectrin-deficient red cells. Blood 115(9):1804-14. [PubMed: 20056793]  [MGI Ref ID J:157766]

Russell ES; Bernstein SE. 1966. Blood and Blood Formation. In: Biology of the Laboratory Mouse. McGraw Hill, New York.  [MGI Ref ID J:24829]

Sassa S; Bernstein SE. 1978. Studies of erythrocyte protoporphyrin in anemic mutant mice: use of a modified hematofluorometer for the detection of heterozygotes for hemolytic disease. Exp Hematol 6(5):479-87. [PubMed: 658175]  [MGI Ref ID J:5985]

Shear HL; Roth EF Jr; Ng C; Nagel RL. 1991. Resistance to malaria in ankyrin and spectrin deficient mice. Br J Haematol 78(4):555-60. [PubMed: 1832936]  [MGI Ref ID J:111131]

Unger AE; Harris MJ; Bernstein SE; Falcone JC; Lux SE. 1983. Hemolytic anemia in the mouse. Report of a new mutation and clarification of its genetics. J Hered 74(2):88-92. [PubMed: 6841965]  [MGI Ref ID J:7048]

Wandersee NJ; Birkenmeier CS; Gifford EJ; Mohandas N; Barker JE. 2000. Murine recessive hereditary spherocytosis, sph/sph, is caused by a mutation in the erythroid alpha-spectrin gene. Hematol J 1(4):235-42. [PubMed: 11920196]  [MGI Ref ID J:80999]

Wandersee NJ; Tait JF; Barker JE. 2000. Erythroid phosphatidyl serine exposure is not predictive of thrombotic risk in mice with hemolytic anemia Blood Cells Mol Dis 26(1):75-83. [PubMed: 10772878]  [MGI Ref ID J:62355]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3300.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery of Strains Needing Progeny Testing
    At least two untested males and two untested females (two pairs) will be recovered (eight or more mice is typical). The total number of animals provided, their gender and genotype will vary. Untested animals typically are available to ship between 10 and 14 weeks from the date of your order. If the first recovery attempt is unsuccessful, a second recovery will be done, extending the overall recovery time to approximately 25 weeks. Progeny testing is required to identify the genotype of mice of this strain, as a genotyping assay is not available. This type of testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. We can perform the progeny testing for you as a service or we can ship all recovered animals to you for progeny testing at your facility. If you perform the progeny testing, there is no guarantee that a carrier will be identified. If we perform progeny testing as a service, additional breeding time will be required. In this case, when a male and female (one pair) are identified that carry the mutation, they and their offspring will be shipped. Delivery time for strains requiring progeny testing often exceeds 25 weeks and may take 12 months or more due to the difficulties in breeding some strains. The progeny testing cost is in addition to the recovery cost and is based on the number of boxes used and the time taken to produce the mice identified as carrying the mutation.
    Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation.

    Please contact Customer Service for more information on the cost of progeny testing for a strain, tel: 1-800-422-6423 or 1-207-288-5845 (from any location). The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $4290.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery of Strains Needing Progeny Testing
    At least two untested males and two untested females (two pairs) will be recovered (eight or more mice is typical). The total number of animals provided, their gender and genotype will vary. Untested animals typically are available to ship between 10 and 14 weeks from the date of your order. If the first recovery attempt is unsuccessful, a second recovery will be done, extending the overall recovery time to approximately 25 weeks. Progeny testing is required to identify the genotype of mice of this strain, as a genotyping assay is not available. This type of testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. We can perform the progeny testing for you as a service or we can ship all recovered animals to you for progeny testing at your facility. If you perform the progeny testing, there is no guarantee that a carrier will be identified. If we perform progeny testing as a service, additional breeding time will be required. In this case, when a male and female (one pair) are identified that carry the mutation, they and their offspring will be shipped. Delivery time for strains requiring progeny testing often exceeds 25 weeks and may take 12 months or more due to the difficulties in breeding some strains. The progeny testing cost is in addition to the recovery cost and is based on the number of boxes used and the time taken to produce the mice identified as carrying the mutation.
    Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation.

    Please contact Customer Service for more information on the cost of progeny testing for a strain, tel: 1-800-422-6423 or 1-207-288-5845 (from any location). The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

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Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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