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General Terms and Conditions

Former Name	B6 x B6CBACa-Aw-J/A-Myo5aflr Gnb5flr    (Changed: 15-DEC-04 )
Genes & Alleles	Aw-J;   Gnb5;   Gnb5flr;   Myo5a;   Myo5aflr;   a;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Species laboratory mouse Generation N1 f3+N1

Strain Development
The flailer mutation arose spontaneously on the C57BL/10J background at The Jackson Laboratory in 1971. It was initially named tumbler 2 Jackson because it resembled the tumbler mutation. However, these mutations are not allelic, and tumbler maps to chromosome 1 while flailer occurred in Chromosome 9. The flailer mutation was maintained for many years by outcrossing a homozygote to B6CBACaF1/J A^w-J/A then intercrossing the obligate heterozygous offspring. In 1983 C57BL/6J females were bred with B6CBACa A^w-J/A-Myo5a^flr Gnb5^flr/J heterozygous males, ranging in generation from N17F1 to N17F3, to generate embryos for cryopreservation.

Mammalian Phenotype Terms assigned by genotype The following phenotype information may relate to a genetic background differing from this JAX® Mice strain. Gnb5flr/Gnb5flr Myo5aflr/Myo5aflr         involves: C57BL/6J * C57BL/10J * CBA/Ca behavior/neurological phenotype abnormal motor capabilities/coordination/movement (MGI Ref ID J:61324) by 14 days of age, mice exhibit frequent falling behavior frequent falls exhibited by 50% of mice ataxia (MGI Ref ID J:61324) adults display mild ataxia 50% of homozygotes are severely ataxic other 50% show transient ataxia at 3 weeks of age impaired balance (MGI Ref ID J:61324) adults cannot maintain balance on a narrow beam impaired coordination (MGI Ref ID J:61324) mice fall off rotating rod within 0.5-1 minute; coordination improves with age impaired righting response (MGI Ref ID J:61324) by 14 days of age, mice display convulsive limb movements during attempts to right themselves legs flail during attempts to right themselves in 50% of animals opisthotonus (MGI Ref ID J:61324) can be stimulated by handling between 2 and 4 weeks of age convulsive seizures (MGI Ref ID J:61324) increase in frequency between 2 and 4 weeks of age nervous system phenotype abnormal cerebellum morphology (MGI Ref ID J:61324) localization of smooth endoplasmic reticulum (SER) vesicles is abnormal in cerebellum of 20-day old mice abnormal Purkinje cell dendrite morphology (MGI Ref ID J:61324) Times New Roman convulsive seizures (MGI Ref ID J:61324) increase in frequency between 2 and 4 weeks of age muscle phenotype opisthotonus (MGI Ref ID J:61324) can be stimulated by handling between 2 and 4 weeks of age pigmentation phenotype *normal* pigmentation phenotype (MGI Ref ID J:61324) mutants have normal pigment indicating C-terminus exons of Myo5a are not involved in the dilute coat color change associated with other Myo5a mutations lethality-postnatal postnatal lethality (MGI Ref ID J:42513) 50% of animals survive only 3 weeks; lethality may be partially background-sensitive

Gene & Allele Details

Allele Symbol		Aw-J
Allele Name		white bellied agouti Jackson
Common Name(s)		AWJ;
Strain of Origin		C57BL/6J
Gene Symbol and Name		a, nonagouti
Chromosome		2
Gene Common Name(s)		AGSW; AGTI; AGTIL; ASP; As; MGC126092; MGC126093; SHEP9; agouti; agouti signal protein; agouti suppressor;
Allele Symbol		Gnb5flr
Allele Name		flailer
Common Name(s)		flail; flr; tb2J;
Strain of Origin		C57BL/10J x B6CBA/Ca
Gene Symbol and Name		Gnb5, guanine nucleotide binding protein (G protein), beta 5
Chromosome		9
Gene Common Name(s)		FLJ37457; FLJ43714; G beta 5; GB5; Gbeta5; MGC105255; flail; flailer; flr;
General Note		The flailer mutation, originally called tb2J, was a presumed remutation of tb because of a similar phenotype. This mutation, however, does not map to Chr 1, and the original unpublished report of linkage of tb to Chr 1 is in doubt. Thus, it isnot clear whether tb2J is a remutation at the tb locus which was not on Chr 1, or if tb2J is a new mutation at a different locus. Because of its linkage to a different chromosome, tb2J was renamed flr. flr mutants have a variable neurological phenotype. Half of all flailer homozygotes show severe ataxia and only live for 3 weeks; these mutants tend to fall over and flail their limbs in an effort to right themselves. The other half of flailer homozygotes, however, only develop a transient ataxia; they recover, are fertile, and have a normal life span.
Molecular Note		Hybrid. This mutation is caused by gene shuffling between Gnb5 and Myo5a. The shuffling combines the promoter of the first two exons of Gnb5 with the C-terminal exons of Myo5a. Flailer homozygotes express transcripts of three genes: wild-type Gnb5, wild-type Myo5a and the flailer hybrid gene. [MGI Ref ID J:61324]
Allele Symbol		Myo5aflr
Allele Name		flailer
Common Name(s)		flail; flr; tb2J;
Strain of Origin		C57BL/10J
Gene Symbol and Name		Myo5a, myosin Va
Chromosome		9
Gene Common Name(s)		9630007J19Rik; AI413174; AI661011; D; Dbv; Dop; GS1; MVa; MYH12; MYO5; MYR12; Myo5; MyoVA; RIKEN cDNA 9630007J19 gene; d; dilute; expressed sequence AI413174; expressed sequence AI661011; flail; flailer; flr; myosin V; nmf244;
Molecular Note		Hybrid. This mutation is caused by gene shuffling between Gnb5 and Myo5a. The shuffling combines the promoter of the first two exons of Gnb5 with the C-terminal exons of Myo5a. Flailer homozygotes express transcripts of three genes: wild-type Gnb5, wild-type Myo5a and the flailer hybrid gene. [MGI Ref ID J:61324]

Genotyping Protocols

A^w-J

Related Strains

Strains carrying   A^w-J allele

000202	AEJ/Gn-bd/J
000199	AEJ/GnLeJ
000593	B6 x B6CBCa Aw-J/A-Grid2Lc T(2;6)7Ca MitfMi-wh/J
000599	B6 x B6CBCa Aw-J/A-T(5;13)264Ca KitW-v/J
002016	B6(Cg)-Aw-J EdaTa-6J Chr YB6-Sxr/EiJ
000552	B6-Aw-J-EdaTa-6J.Cg-Sxr
001730	B6-Aw-J-EdaTa-6J.Cg-Sxrb Hya-/J
000841	B6-Aw-J.CBy-EdaTa-By/J
001809	B6-Aw-J.Cg-EdaTa-6J +/+ ArTfm/J
000600	B6-Gpi1b x B6CBCa Aw-J/A-T(7;15)9H Gpi1a/J
100409	B6129PF1/J-Aw-J/Aw
004200	B6;CBACa Aw-J/A-Npr2cn-2J/J
000505	B6C3 Aw-J/A-Mutedmu/J
000314	B6CBACa Aw-J/A-EdaTa/J-XO
000501	B6CBACa Aw-J/A-Aifm1Hq/J
001046	B6CBACa Aw-J/A-Grid2Lc/J
000500	B6CBACa Aw-J/A-Gs/J
002703	B6CBACa Aw-J/A-Hydinhy3/J
000247	B6CBACa Aw-J/A-Kcnj6wv/J
000287	B6CBACa Aw-J/A-Plp1jp EdaTa/J
000515	B6CBACa Aw-J/A-SfnEr/J
000242	B6CBACa Aw-J/A-spc/J
000288	B6CBACa Aw-J/A-we a Mafbkr/J
001201	B6CBACaF1/J-Aw-J/A
001752	B6CBCa Aw-J/A-T(7;15)9H/J
000200	C3FeB6 A/Aw-J-Ankank/J
000638	C3FeB6 A/Aw-J-Spnb4qv-J/J
001203	C3FeB6F1/J A/Aw-J
000338	C57BL/6J Aw-J-EdaTa-6J/J
000569	C57BL/6J-Aw-J-EdaTa +/+ ArTfm/J
000051	C57BL/6J-Aw-J/J

View Strains carrying   A^w-J     (31 strains)

Strains carrying other alleles of Myo5a

005012	A.B6 Tyr+-Myo5ad-l31J/J
001005	AKXD1/TyJ
001003	AKXD11/TyJ
000765	AKXD13/TyJ
000779	AKXD14/TyJ
000954	AKXD15/TyJ
001093	AKXD18/TyJ
000776	AKXD2/TyJ
001062	AKXD21/TyJ
000947	AKXD22/TyJ
000949	AKXD25/TyJ
000764	AKXD27/TyJ
000959	AKXD3/TyJ
001013	B10.D2/nSnJ-Myo5ad-n/J
000285	B6.Cg-Rorasg + +/+ Myo5ad Bmp5se/J
000652	BDP/J
000036	BXD1/TyJ
000013	BXD16/TyJ
000015	BXD18/TyJ
000010	BXD19/TyJ
000077	BXD21/TyJ
000043	BXD22/TyJ
000081	BXD25/TyJ
006255	BXD25/TyJRwwJ
000029	BXD29/TyJ
000037	BXD5/TyJ
000007	BXD6/TyJ
000084	BXD8/TyJ
000105	BXD9/TyJ
000284	CWD/LeJ
000670	DBA/1J
000671	DBA/2J
000963	DBA/2J-Myo5ad+17J/Myo5ad/J
000964	DBA/2J-Myo5ad+18J/Myo5ad/J
000067	DBA/2J-Myo5ad+2J/Myo5ad/J
000253	DLS/LeJ
000673	HRS/J
000674	I/LnJ
001850	MEV-Q/TyJ
001855	MEV-V/TyJ
003345	MEV/2Ty-Emv64/J
000679	P/J
000644	SEA/GnJ
000390	STOCK Myo5ad Ds/J
000994	STOCK a Myo5ad Mregdsu/J
000286	STOCK a/a Myo5ad fd/+ +/J

View Strains carrying other alleles of Myo5a     (46 strains)

Strains carrying other alleles of a

003301	(C57BL/6J x C3H-Eya1bor)F1/J
000251	AEJ.Cg-ae +/a Gdf5bp-H/J
000202	AEJ/Gn-bd/J
000199	AEJ/GnLeJ
000427	B10.CE-H13b Aw/(30NX)SnJ
000420	B10.LP-H13b Aw/Sn
000477	B10.PA-Pldnpa H3e at/SnJ
000419	B10.UW-H3b we Pax1un at/SnJ
003879	B10;TFLe-a/a T tf/+ tf/J
001538	B6 x B6C3Sn a/A-T(1;9)27H/J
000916	B6 x B6C3Sn a/A-T(5;12)31H/J
000602	B6 x B6C3Sn a/A-T(8;16)17H/J
002083	B6 x B6EiC3 a/A-T(7;16)235Dn/J
000507	B6 x B6EiC3 a/A-Otcspf/J
000618	B6 x FSB/GnEi a/a Ctslfs/J
000577	B6 x STOCK a Oca2p Hps5ru2 Ednrbs/J
000601	B6 x STOCK a/a T(7;18)50H/J
000592	B6 x STOCK T(2;4)13H a/J
000769	B6.C/(HZ18)By-at-44J/J
000001	B6.C3 A/a Mgrn1md/J
000203	B6.C3-Aiy/a/J
000017	B6.C3Fe-Avy/J
000628	B6.CE-A Amy1b Amy2b/J
005505	B6.Cg-Ay Slc7a11sut/LmLlp
000021	B6.Cg-Ay/J
001572	B6.Cg-am-J/J
004200	B6;CBACa Aw-J/A-Npr2cn-2J/J
000785	B6;D2-a Es1e/J
000604	B6C3 a/A-T(10;13)199H +/+ Lystbg-J/J or Lystbg-2J/J
002807	B6C3Fe a/a-Meox2fla/J
000224	B6C3Fe a/a-Scyl1mdf/J
001037	B6C3Fe a/a-Agtpbp1pcd/J
000221	B6C3Fe a/a-Alx4lst-J/J
002062	B6C3Fe a/a-Atp7aMo-8J/J
001756	B6C3Fe a/a-Cacng2stg/J
001815	B6C3Fe a/a-Col1a2oim/J
000231	B6C3Fe a/a-Csf1op/J
000209	B6C3Fe a/a-Dh/J
000211	B6C3Fe a/a-Dstdt-J/J
000210	B6C3Fe a/a-Edardl-J/J
000207	B6C3Fe a/a-Edaraddcr/J
000182	B6C3Fe a/a-Eef1a2wst/J
001278	B6C3Fe a/a-Glra1spd/J
000241	B6C3Fe a/a-Glrbspa/J
002875	B6C3Fe a/a-Hoxd13spdh/J
000304	B6C3Fe a/a-Krt71Ca Scn8amed-J/J
000226	B6C3Fe a/a-Largemyd/J
000636	B6C3Fe a/a-Lmx1adr-J/J
001280	B6C3Fe a/a-Lse/J
001573	B6C3Fe a/a-MitfMi/J
001035	B6C3Fe a/a-Napahyh/J
000181	B6C3Fe a/a-Otogtwt/J
000278	B6C3Fe a/a-Papss2bm Hps1ep Hps6ru/J
000205	B6C3Fe a/a-Papss2bm/J
002078	B6C3Fe a/a-Pcdh15av-2J/J
000246	B6C3Fe a/a-Pitpnavb/J
001430	B6C3Fe a/a-Ptch1mes/J
000506	B6C3Fe a/a-Qkqk/J
000235	B6C3Fe a/a-Relnrl/J
000237	B6C3Fe a/a-Rorasg/J
000290	B6C3Fe a/a-Sox10Dom/J
000230	B6C3Fe a/a-Tcirg1oc/J
003612	B6C3Fe a/a-Trak1hyrt/J
001512	B6C3Fe a/a-Ttnmdm/J
001607	B6C3Fe a/a-Unc5crcm/J
000005	B6C3Fe a/a-Wc/J
000243	B6C3Fe a/a-Wnt1sw/J
000248	B6C3Fe a/a-Xpl/J
001750	B6C3Fe a/a-XsJ/J
000624	B6C3Fe a/a-anx/J
003020	B6C3Fe a/a-dep/J
002018	B6C3Fe a/a-din/J
002339	B6C3Fe a/a-nma/J
000240	B6C3Fe a/a-soc/J
000063	B6C3Fe a/a-sy/J
001055	B6C3Fe a/a-tip/J
000245	B6C3Fe a/a-tn/J
000065	B6C3Fe a/a-we Pax1un at/J
000296	B6C3Fe-a/a Hoxa13Hd Mcoln3Va-J/J
000019	B6C3Fe-a/a-Itpr1opt/J
001022	B6C3FeF1/J a/a
001752	B6CBCa Aw-J/A-T(7;15)9H/J
000971	B6EiC3 a/A-Och/J
000551	B6EiC3 a/A-Tbx15de-H/J
006450	B6EiC3 a/A-Vss/J
000557	B6EiC3-+ a/LnpUl A/J
000504	B6EiC3Sn a/A-Cacnb4lh/J
000553	B6EiC3Sn a/A-Egfrwa2 Wnt3avt/J
000503	B6EiC3Sn a/A-Gy/J
001811	B6EiC3Sn a/A-Otcspf-ash/J
002343	B6EiC3Sn a/A-Otcspf/J
000391	B6EiC3Sn a/A-Pax6Sey-Dey/J
001924	B6EiC3Sn a/A-Ts(1716)65Dn
001923	B6EiC3Sn a/A-Ts(417)2Lws Tim/J
000200	C3FeB6 A/Aw-J-Ankank/J
000638	C3FeB6 A/Aw-J-Spnb4qv-J/J
000225	C3FeLe.B6 a/a-Ptpn6me/J
000198	C3FeLe.B6-a/J
000291	C3FeLe.Cg-a/a Hm KitlSl Krt71Ca-J/J
001272	C3H/HeSnJ-Ahvy/J
000099	C3HeB/FeJ-Avy/J
001886	C3HeB/FeJLe a/a-gnd/J
000584	C57BL/6J-+ T(1;2)5Ca/a +/J
000258	C57BL/6J-Ai/a/J
000774	C57BL/6J-Asy/a/J
000055	C57BL/6J-at-33J/J
000070	C57BL/6J-atd/J
000284	CWD/LeJ
000670	DBA/1J
000671	DBA/2J
001057	HPT/LeJ
000260	JGBF/LeJ
002468	KK.Cg-Ay/J
000262	LS/LeJ
000283	LT.CAST-A/J
000265	MY/HuLeJ
000308	SSL/LeJ
001759	STOCK A Tyrc Sha/J
001427	STOCK Aw us/J
000994	STOCK a Myo5ad Mregdsu/J
000064	STOCK a Tyrp1b Sisi/J
002238	STOCK a Tyrp1b shmy/J
001433	STOCK a skt/J
000579	STOCK a tp/J
000319	STOCK a us/J
002648	STOCK a/a Cln6nclf/J
000317	STOCK a/a Egfrwa2/J
000302	STOCK a/a MitfMi-wh +/+ Itpr1opt/J
000286	STOCK a/a Myo5ad fd/+ +/J
000206	STOCK a/a Tyrc-h/J
001432	STOCK a/a Tyrp1b sks/Tyrp1b +/J
000281	STOCK a/a ma ft/ma ft/J
000312	STOCK stb + a/+ Fignfi a/J
000596	STOCK T(2;11)30H/+ x AEJ-a Gdf5bp-H/J or A/J-a Gdf5bp-J/J
000970	STOCK T(2;16)28H A/T(2;16)28H a/J
000590	STOCK T(2;4)1Sn a/J
000594	STOCK T(2;8)26H a/T(2;8)26H a Tyrp1+/Tyrp1b/J
000623	TR/DiEiJ

View Strains carrying other alleles of a     (138 strains)

Research Applications

This mouse can be used to support research in many areas including:

Gnb5^flr related

Neurobiology Research
Ataxia (Movement) Defects

Myo5a^flr related

Ataxia (Movement) Defects

References

Additional References

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Price and Supply Information

Strain Name:	B6 x B6CBCa Aw-J/A-Myo5aflr Gnb5flr/J
Stock Number:	000502

Price Details

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Supply Details

Standard Supply	Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes	Cryorecovery of Strains Needing Progeny Testing. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two untested males and two untested females (two pairs) will be recovered, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the overall recovery time to approximately 25 weeks. However, all pups recovered will be sent. Progeny testing is required to identify the genotype of mice of this strain, as a genotyping assay is not available. This type of testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. We can perform the progeny testing for you as a service or we can ship all recovered animals (at least two untested pairs) to you for progeny testing at your facility. If you perform the progeny testing, there is NO guarantee that a carrier will be identified. If we perform progeny testing as a service, additional breeding time will be required. In this case, when a male and female (one pair) are identified that carry the mutation, they and their offspring will be shipped. Delivery time for strains requiring progeny testing often exceeds 25 weeks and may take 12 months or more due to the difficulties in breeding some strains. The progeny testing cost is in addition to the recovery cost and is based on the number of boxes used and the time taken to produce the mice identified as carrying the mutation. Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Please contact Customer Service for more information on the cost of progeny testing for a strain: Tel: 1-800-422-6423 or 1-207-288-5845. Cryorecovery to establish a Dedicated Supply for greater quantities of mice One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org. Genomic DNA is available for this strain from the Mouse DNA Resource.
Licensing	See General Terms and Conditions below

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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