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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names C3H/HeJ-Lystbg-2J/J (Changed: 17-JUN-09 ) Type Coisogenic; Mutant Strain; Spontaneous Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation F132+114p F1 (03-JUL-05) Appearance
light agouti, affected
Related Genotype: A/A Lystbg-2J/Lystbg-2J
agouti, unaffected
Related Genotype: A/A Lystbg-2J/+Important Note
This strain is homozygous for the retinal degeneration allele Pde6brd1.Description
Mice homozygous for the beige 2J spontaneous mutation (Lystbg-2J) display characteristics very similar to the original beige mutation (Lystbg). The mouse phenotype closely resembles Chediak-Higashi syndrome in man and similar conditions in mink and cattle. Abnormal giant lysosomal granules occur in all tissues with granule-containing cells, including granulocytes, lymphocytes, cells of the liver, kidney, central nervous system, pancreas, and thyroid, and the ducts of most glands; in type II pneumocytes; in mast cells; and in retinal pigment epithelium. Granulocytes from beige homozygous mutant mice show defective chemotaxis and reduced bactericidal activity. Beige mice are more susceptible than controls to pneumonitis and to various viral, bacterial, and parasitic infections. Natural killer (NK) cells from beige mice exhibit decreased endogenous cytotoxic activity. Beige mice also have a defective cytotoxic T-cell and cytotoxic antibody response to allogeneic tumor cells. Syngeneic tumor cells grow better in beige mice than in littermate controls, an effect thought to be due to the deficiency of NK cells. Beige mice have platelet storage pool deficiency, leading to a prolonged bleeding time. The immunodeficiency of beige mutant mice has been used, especially in combination with the scid mutation (Prkdcscid), in tissue graft and disease studies.
Control Information
| Control | ||
|---|---|---|
| Heterozygote from the colony | ||
| 000659 C3H/HeJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Lystbg-2J allele
000604 B6C3 a/A-T(10;13)199H +/+ Lystbg-J/J or Lystbg-2J/J View Strains carrying Lystbg-2J (1 strain)
000204 B6.C3Rl-Lystbg/J 000604 B6C3 a/A-T(10;13)199H +/+ Lystbg-J/J or Lystbg-2J/J 000629 C57BL/6J-Lystbg-J/J 000494 J.Cg-Oca2+ Tyr+ Lystbg/J 000269 SB/LeJ
004297 B6.CXB1-Pde6brd10/J 003647 B6EiC3Sn.BLiAF1 002802 C3.BLiA Pde6b+-Krd/J 001979 C3A.BLiA-Pde6b+.O20-Prph2Rd2/J 001912 C3A.BLiA-Pde6b+/J 003648 C3Sn.BLiA-Pde6b+/Dn 004766 C57BL/6J-Pde6brd1-2J/J 004828 FVB.129P2-Pde6b+ Tyrc-ch/AntJ 004808 STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
Additional Web Information
JAX® NOTES, Spring 1991; 445. Why C57BL/6J-bgJ (beige) Mice are not Beige.
JAX® NOTES, Winter 1992; 448. The Beige (BgJ) Mutation.
Phenotype
Phenotype Information
assigned by genotype
Lystbg-2J/Lystbg-2J
C3.Cg-Lystbg-2J/J
- immune system phenotype
- altered susceptibility to infection (MGI Ref ID J:147940)
- decreased susceptibility to fungal infection (MGI Ref ID J:147940)
- mice are less susceptible to experimental cryptococcosis infection than mice homozygous for the Lystbg-J allele on C57BL/6
- tumorigenesis
- altered metastatic potential (MGI Ref ID J:33522)
- decreased metastatic potential (MGI Ref ID J:33522)
- mutant mice had a significantly higher retention of the introduced tumor variant, CIRAS 1, than normal mice after an exercise regime or sedentary activity
- mutant mice randomly assigned 9 week treadmill training had lower CIRAS 1 tumor cell retention (44 +/- 3%) than mice displaying sedentary behavior (53 +/- 3%); there was no difference seen for the more aggressive tumor variant CIRAS 3
- behavior/neurological phenotype
- abnormal gait (MGI Ref ID J:15166)
- an impaired gait is obvious by 17 months of age
- impaired balance (MGI Ref ID J:15166)
- obvious by 17 months of age
- impaired swimming (MGI Ref ID J:15166)
- mice lose ability to swim by 17 months of age
- tremors (MGI Ref ID J:15166)
- a fine sustained tremor is observed by 17 months of age
- muscle phenotype
- muscle weakness (MGI Ref ID J:15166)
- motor weakness of the hind legs is noticeable by 17 months of age
- nervous system phenotype
- decreased Purkinje cell number (MGI Ref ID J:15166)
- there is a complete or nearly complete loss of cells in mice showing neurological symptoms by 17 months of age
- mice in their second year of life and without obvious abnormal neurological behavior have a significant reduction in number of cells
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Lystbg-2J/Lystbg-2J
Background Not Specified
- pigmentation phenotype
- abnormal ear pigmentation (MGI Ref ID J:65327)
- pigmentation is reduced
- abnormal eye pigmentation (MGI Ref ID J:65327)
- eye color is light at birth, varying from ruby to almost black in adults
- reduced eye pigmentation (MGI Ref ID J:65327)
- abnormal tail pigmentation (MGI Ref ID J:65327)
- pigmentation is reduced
- diluted coat color (MGI Ref ID J:65327)
- coat color is lighter than that of normal wild-type mice of the same background
- skin/coat/nails phenotype
- abnormal ear pigmentation (MGI Ref ID J:65327)
- pigmentation is reduced
- abnormal tail pigmentation (MGI Ref ID J:65327)
- pigmentation is reduced
- diluted coat color (MGI Ref ID J:65327)
- coat color is lighter than that of normal wild-type mice of the same background
- craniofacial phenotype
- abnormal ear pigmentation (MGI Ref ID J:65327)
- pigmentation is reduced
- hearing/vestibular/ear phenotype
- abnormal ear pigmentation (MGI Ref ID J:65327)
- pigmentation is reduced
- limbs/digits/tail phenotype
- abnormal tail pigmentation (MGI Ref ID J:65327)
- pigmentation is reduced
- vision/eye phenotype
- abnormal eye pigmentation (MGI Ref ID J:65327)
- eye color is light at birth, varying from ruby to almost black in adults
- reduced eye pigmentation (MGI Ref ID J:65327)
This mouse can be used to support research in many areas including:Lystbg-2J related
Pde6brd1 relatedCardiovascular Research
Atherosclerosis
Dermatology Research
Color and White Spotting Defects
Hematological Research
Immunological Defects
Platelet Defects
platelet storage pool deficiency
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
Intracellular Signaling Molecules
Internal/Organ Research
Kidney Defects
lysosomal enzyme abnormalities
Metabolism Research
Mouse/Human Gene Homologs
Chediak-Higashi syndrome
Research Tools
Immunology and Inflammation Research
NK Cell Deficiency
Mouse/Human Gene Homologs
retinitis pigmentosa, autosomal recessive
Sensorineural Research
Retinal Degeneration
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Lystbg-2J | ||
|---|---|---|---|
| Allele Name | beige 2 Jackson | ||
| Allele Type | Spontaneous | ||
| Strain of Origin | Yellow stock | ||
| Gene Symbol and Name | Lyst, lysosomal trafficking regulator | ||
| Chromosome | 13 | ||
| Gene Common Name(s) | Beige; CHS; CHS1; D13Sfk13; DNA segment, Chr 13, Stephen F. Kingsmore 13; beige; bg; | ||
| Molecular Note | This allele was identified by a noncomplementation test with Lystbg. Quantitative reverse transcription showed the molecular fault in the Lystbg-2J mutation may be an intronic retrotransposition event causing a five-fold decrease intranscription and mRNA instability. [MGI Ref ID J:34205] | ||
| Allele Symbol | Pde6brd1 | ||
| Allele Name | retinal degeneration 1 | ||
| Allele Type | Spontaneous | ||
| Common Name(s) | Pdebrd1; rd; rd-1; rd1; rodless retina; | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Genotyping resources and troubleshooting
References
References
Additional References
Gallin JI; Bujak JS; Patten E; Wolff SM. 1974. Granulocyte function in the Chediak-Higashi syndrome of mice. Blood 43(2):201-6. [PubMed: 4589319] [MGI Ref ID J:5405]
Perou CM; Moore KJ; Nagle DL; Misumi DJ; Woolf EA; McGrail SH; Holmgren L; Brody TH; Dussault BJ Jr; Monroe CA; Duyk GM; Pryor RJ; Li L; Justice MJ; Kaplan J. 1996. Identification of the murine beige gene by YAC complementation and positional cloning. Nat Genet 13(3):303-8. [PubMed: 8673129] [MGI Ref ID J:33734]
Roder JC. 1979. The beige mutation in the mouse. I. A stem cell predetermined impairment in natural killer cell function. J Immunol 123(5):2168-73. [PubMed: 489978] [MGI Ref ID J:6213]
Schiller NK; Black AS; Bradshaw GP; Bonnet DJ; Curtiss LK. 2004. Participation of macrophages in atherosclerotic lesion morphology in LDLr-/- mice. J Lipid Res 45(8):1398-409. [PubMed: 15175354] [MGI Ref ID J:91367]
Lystbg-2J relatedBarbosa MD; Nguyen QA; Tchernev VT; Ashley JA; Detter JC; Blaydes SM; Brandt SJ; Chotai D; Hodgman C; Solari RC; Lovett M; Kingsmore SF. 1996. Identification of the homologous beige and Chediak-Higashi syndrome genes. Nature 382(6588):262-5. [PubMed: 8717042] [MGI Ref ID J:34205]
Jadeski L; Hoffman-Goetz L. 1996. Exercise and in vivo natural cytotoxicity against tumour cells of varying metastatic capacity. Clin Exp Metastasis 14(2):138-44. [PubMed: 8605727] [MGI Ref ID J:33522]
Lane PW. 1962. bg-beige and bg<2J> - beige-2J Mouse News Lett 26:35. [MGI Ref ID J:65327]
Marquis G; Montplaisir S; Pelletier M; Mousseau S; Auger P. 1985. Genetic resistance to murine cryptococcosis: the beige mutation (Chediak-Higashi syndrome) in mice. Infect Immun 47(1):288-93. [PubMed: 3965401] [MGI Ref ID J:147940]
Murphy ED; Roths JB. 1978. Purkinje cell degeneration, a late effect of beige mutations in mice Jackson Lab Ann Rep 49:108-9. [MGI Ref ID J:15166]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
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| Important Note | |
| This strain is homozygous for the retinal degeneration allele Pde6brd1. | |
Control Information
| Control | ||
|---|---|---|
| Heterozygote from the colony | ||
| 000659 C3H/HeJ | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
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See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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