Strain Name: |
C3H/HeJ-Pou1f1dw-J/J |
|---|---|
Stock Number: |
000510 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
C3H/HeJ-Pit1dw-J/J (Changed: 21-SEP-06
) |
| Genes & Alleles | Pou1f1; Pou1f1dw-J; |
Type JAX® GEMM® Strain - Coisogenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Spontaneous Mutation Species laboratory mouse Generation N7 Strain Description
Mice homozygous mice for the dwarf Jackson spontaneous mutation (Pou1f1dw-J) have a phenotype very similar to mice homozygous for the original dwarf mutation (Pou1f1dw). Homozygous mutant mice are characterized by severe proportional dwarfing, sterility, and hypothyroidism. Adult dwarf mice are about one-fourth to one-third the size of wildtype mice. There is a lack of growth hormone, prolactin and thyroid stimulating hormone producing cells in the anterior pituitary leading to severe endocrine deficiency of these hormones. Homozygous mutant mice show a transient loss in cortical thymocytes associated with the primary defect in anterior pituitary.Strain Development
The dwarf Jackson mutation (Pou1f1dw) arose spontaneously in strain C3H/HeJ at generation F136 in 1973 at The Jackson Laboratory. It was maintained by mating heterozygous tested sibling pairs until generation F15. It was then maintained by ovarian transplant using the cross-intercross system to generation N6 and then again by tested heterozygous pair matings. It was cryopreserved in 1983 by mating heterozygous (Pou1f1dw-J/J) males at generation F136+16N6F1 to C3H/HeJ females.
Mammalian Phenotype Terms assigned by genotype |
| Allele Symbol | Pou1f1dw-J | ||
|---|---|---|---|
| Allele Name | dwarf Jackson | ||
| Common Name(s) | Pit1dwJ; dwJ; dwarf-J; | ||
| Strain of Origin | C3H/HeJ | ||
| Gene Symbol and Name | Pou1f1, POU domain, class 1, transcription factor 1 (Pit1, growth hormone factor 1) | ||
| Chromosome | 16 | ||
| Gene Common Name(s) | GHF-1; GHF1; GHF1A; Hmp1; PIT1; PIT1Z; Pit-1; Pit1; Pit1-rs1; Snell dwarf; dw; dwarf; pituitary specific transcription factor 1; pituitary specific transcription factor 1, related sequence 1; | ||
| General Note | This remutation of the Snell dwarf gene occurred in the C3H/HeJ strain (J:6342). Mutant mice homozygous for Pit1dw-J, like homozygotes for Pit1dw, lack PIT1 protein activity and have no lactotrophs, thyrotrophs, or somatotrophs (J:10774). | ||
| Molecular Note | The mutation was characterized by Southern blot as either a chromosomal inversion or an insertion of greater than 4kb in the gene. PCR analysis revealed that most of exon 3 is intact, but the exact break points are not established. [MGI Ref ID J:10774] | ||
| Control | ||
|---|---|---|
| Untyped from the colony | ||
| 000659 C3H/HeJ | ||
| Considerations for Choosing Controls | ||
Strains carrying other alleles of Pou1f1
000772 B6.DW-Pou1f1dw/J 000681 DW.C3-Mlph+ Pou1f1+/J 000643 DW/J Mlphln Pou1f1dw/J View Strains carrying other alleles of Pou1f1 (3 strains)
Pou1f1dw-J related
Developmental Biology Research
Growth Defects
Endocrine Deficiency Research
Hypothalamus/Pituitary Defects
Immunology and Inflammation Research
Immunodeficiency Associated with Other Defects
Mouse/Human Gene Homologs
pituitary hormone deficiency
Reproductive Biology Research
Fertility Defects
Selected Reference(s)
Additional ReferencesBoylston WH; Gerstner A; DeFord JH; Madsen M; Flurkey K; Harrison DE; Papaconstantinou J. 2004. Altered cholesterologenic and lipogenic transcriptional profile in livers of aging Snell dwarf (Pit1dw/dwJ) mice. Aging Cell 3(5):283-96. [PubMed: 15379852] [MGI Ref ID J:109839]
Papaconstantinou J; Deford JH; Gerstner A; Hsieh CC; Boylston WH; Guigneaux MM; Flurkey K; Harrison DE. 2005. Hepatic gene and protein expression of primary components of the IGF-I axis in long lived Snell dwarf mice. Mech Ageing Dev 126(6-7):692-704. [PubMed: 15888324] [MGI Ref ID J:98300]
| Strain Name: | C3H/HeJ-Pou1f1dw-J/J |
| Stock Number: | 000510 |
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery of Strains Needing Progeny Testing. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two untested males and two untested females (two pairs) will be recovered, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the overall recovery time to approximately 25 weeks. However, all pups recovered will be sent. Progeny testing is required to identify the genotype of mice of this strain, as a genotyping assay is not available. This type of testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. We can perform the progeny testing for you as a service or we can ship all recovered animals (at least two untested pairs) to you for progeny testing at your facility. If you perform the progeny testing, there is NO guarantee that a carrier will be identified. If we perform progeny testing as a service, additional breeding time will be required. In this case, when a male and female (one pair) are identified that carry the mutation, they and their offspring will be shipped. Delivery time for strains requiring progeny testing often exceeds 25 weeks and may take 12 months or more due to the difficulties in breeding some strains. The progeny testing cost is in addition to the recovery cost and is based on the number of boxes used and the time taken to produce the mice identified as carrying the mutation. Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Please contact Customer Service for more information on the cost of progeny testing for a strain: Tel: 1-800-422-6423 or 1-207-288-5845. Cryorecovery to establish a Dedicated Supply for greater quantities of mice |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
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