Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Coisogenic; Mutant Strain; Spontaneous Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation F85+5N8F1N1

Control Information

	Control
	Untyped from the colony
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Glra1

001278	B6C3Fe a/a-Glra1spd/J
004109	C57BL/6J-Glra1nmf11/J

View Strains carrying other alleles of Glra1     (2 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Glra1^spd-ot/Glra1^spd-ot

        C57BL/6J-Glra1^spd-ot Mpzl3^rc

• lethality-postnatal
• postnatal lethality (MGI Ref ID J:13463)
  • usually die about 22-23 days of age
• behavior/neurological phenotype
• tremors (MGI Ref ID J:13463)
  • begin to tremble violently and very rapidly at about 14 days of age; severity of tremor increases daily
  • at 19-21 days of age, mutants have prolonged periods of great rigor or stiffness; therapy with aminooxyacetic acid is helpful but only temporarily

Glra1^spd-ot/Glra1^spd-ot

        involves: C57BL/6J

• lethality-postnatal
• postnatal lethality (MGI Ref ID J:107942)
  • homozygotes die by the end of the third week after birth
• nervous system phenotype
• *normal* nervous system phenotype (MGI Ref ID J:132622)
  • mice exhibit normal spontaenous glycinergic inhibitory postsynaptic currents in amacrine cells
• abnormal nervous system electrophysiology (MGI Ref ID J:107942)
  • phrenic nerve discharge patterns of mutant mice vary with respect to burst frequency and duration of expiration and inspiration compared to wild-type mice; mutants displayed more frequent phrenic nerve discharges with shorter, less regular expiratory intervals
• decreased neuron number (MGI Ref ID J:107942)
  • in mutants there is a reduction or absence of post-inspiratory neurons compared to wild-type
• muscle phenotype
• muscle spasm (MGI Ref ID J:107942)
  • after rapid breathing episodes, single or multiple whole-body spasms occur with intervals between spasms of 6 seconds to more than 1 minute
• respiratory system phenotype
• abnormal breathing frequency (MGI Ref ID J:107942)
  • conscious mutants (P16-23) display disturbed respiratory patterns characterized by periods of slow breathing and intermittent episodes of rapid breathing; episodes are of variable (2-10 sec)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Glra1^spd-ot related

Cell Biology Research
Channel and Transporter Defects (chloride: glycine receptor)

Mouse/Human Gene Homologs
Kok disease-hyperexplexia, startle disease

Neurobiology Research
Channel and Transporter Defects (chloride: glycine receptor)
Neurotransmitter Receptor and Synaptic Vesicle Defects
Receptor Defects
Tremor Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Glra1spd-ot
Allele Name		spasmodic oscillator
Allele Type		Spontaneous
Common Name(s)		ot;
Strain of Origin		C57BL/6J-Mpzl3
Gene Symbol and Name		Glra1, glycine receptor, alpha 1 subunit
Chromosome		11
Gene Common Name(s)		B230397M16Rik; GLYRA1; MGC138878; MGC138879; RIKEN cDNA B230397M16 gene; STHE; neuroscience mutagenesis facility, 11; nmf11; oscillator; ot; spasmodic; spd;

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Additional References

Buckwalter MS; Cook SA; Davisson MT; White WF; Camper SA. 1994. A frameshift mutation in the mouse alpha 1 glycine receptor gene (Glra1) results in progressive neurological symptoms and juvenile death. Hum Mol Genet 3(11):2025-30. [PubMed: 7874121]  [MGI Ref ID J:21529]

Findlay GS; Phelan R; Roberts MT; Homanics GE; Bergeson SE; Lopreato GF; Mihic SJ; Blednov YA; Harris RA. 2003. Glycine receptor knock-in mice and hyperekplexia-like phenotypes: comparisons with the null mutant. J Neurosci 23(22):8051-9. [PubMed: 12954867]  [MGI Ref ID J:85341]

Glra1^spd-ot related

Buckwalter MS; Cook SA; Davisson MT; White WF; Camper SA. 1994. A frameshift mutation in the mouse alpha 1 glycine receptor gene (Glra1) results in progressive neurological symptoms and juvenile death. Hum Mol Genet 3(11):2025-30. [PubMed: 7874121]  [MGI Ref ID J:21529]

Busselberg D; Bischoff AM; Becker K; Becker CM; Richter DW. 2001. The respiratory rhythm in mutant oscillator mice. Neurosci Lett 316(2):99-102. [PubMed: 11742725]  [MGI Ref ID J:107942]

Cook SA. 1993. Oscillator (ot) Mouse Genome 91(1):117.  [MGI Ref ID J:4292]

Dickie MM. 1967. Oscillator. Mouse News Lett 36:39.  [MGI Ref ID J:13463]

Findlay GS; Phelan R; Roberts MT; Homanics GE; Bergeson SE; Lopreato GF; Mihic SJ; Blednov YA; Harris RA. 2003. Glycine receptor knock-in mice and hyperekplexia-like phenotypes: comparisons with the null mutant. J Neurosci 23(22):8051-9. [PubMed: 12954867]  [MGI Ref ID J:85341]

Graham BA; Schofield PR; Sah P; Callister RJ. 2003. Altered inhibitory synaptic transmission in superficial dorsal horn neurones in spastic and oscillator mice. J Physiol 551(Pt 3):905-16. [PubMed: 12837931]  [MGI Ref ID J:105485]

Graham BA; Schofield PR; Sah P; Margrie TW; Callister RJ. 2006. Distinct physiological mechanisms underlie altered glycinergic synaptic transmission in the murine mutants spastic, spasmodic, and oscillator. J Neurosci 26(18):4880-90. [PubMed: 16672662]  [MGI Ref ID J:108318]

Ivanova E; Muller U; Wassle H. 2006. Characterization of the glycinergic input to bipolar cells of the mouse retina. Eur J Neurosci 23(2):350-64. [PubMed: 16420443]  [MGI Ref ID J:105396]

Kling C; Koch M; Saul B; Becker CM. 1997. The frameshift mutation oscillator (Glra1(spd-ot)) produces a complete loss of glycine receptor alpha1-polypeptide in mouse central nervous system. Neuroscience 78(2):411-7. [PubMed: 9145798]  [MGI Ref ID J:42647]

Markstahler U; Kremer E; Kimmina S; Becker K; Richter DW. 2002. Effects of functional knock-out of alpha 1 glycine-receptors on breathing movements in oscillator mice. Respir Physiol Neurobiol 130(1):33-42. [PubMed: 12380014]  [MGI Ref ID J:102716]

Simon ES. 1997. Phenotypic heterogeneity and disease course in three murine strains with mutations in genes encoding for alpha 1 and beta glycine receptor subunits. Mov Disord 12(2):221-8. [PubMed: 9087981]  [MGI Ref ID J:41909]

Weiss J; O'Sullivan GA; Heinze L; Chen HX; Betz H; Wassle H. 2008. Glycinergic input of small-field amacrine cells in the retinas of wildtype and glycine receptor deficient mice. Mol Cell Neurosci 37(1):40-55. [PubMed: 17920294]  [MGI Ref ID J:132622]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery of Strains Needing Progeny Testing. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two untested males and two untested females (two pairs) will be recovered, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the overall recovery time to approximately 25 weeks. However, all pups recovered will be sent. Progeny testing is required to identify the genotype of mice of this strain, as a genotyping assay is not available. This type of testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. We can perform the progeny testing for you as a service or we can ship all recovered animals (at least two untested pairs) to you for progeny testing at your facility. If you perform the progeny testing, there is NO guarantee that a carrier will be identified. If we perform progeny testing as a service, additional breeding time will be required. In this case, when a male and female (one pair) are identified that carry the mutation, they and their offspring will be shipped. Delivery time for strains requiring progeny testing often exceeds 25 weeks and may take 12 months or more due to the difficulties in breeding some strains. The progeny testing cost is in addition to the recovery cost and is based on the number of boxes used and the time taken to produce the mice identified as carrying the mutation. Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Please contact Customer Service for more information on the cost of progeny testing for a strain: Tel: 1-800-422-6423 or 1-207-288-5845. Cryorecovery to establish a Dedicated Supply for greater quantities of mice One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Untyped from the colony
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.