Strain Name:

DBA/2J-Grid2ho-4J/J

Stock Number:

000548

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Availability:

Cryopreserved - Ready for recovery

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Coisogenic; Mutant Strain; Spontaneous Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse

Appearance
dilute brown
Related Genotype: a/a, Myo5d/Myo5d, Tyrp1b/Tyrp1b

Control Information

  Control
   Heterozygote from the colony
   000671 DBA/2J
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Grid2     (6 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Grid2ho-4J/Grid2ho-4J

        DBA/2J-Grid2ho-4J/J
  • hearing/vestibular/ear phenotype
  • reduced linear vestibular evoked potential
    • elevated threshold and reduced amplitudes   (MGI Ref ID J:116914)
  • behavior/neurological phenotype
  • abnormal locomotor coordination
    • tapping of the hindpaws; phenotype is much less severe than in Grid2ho-Nancy mice   (MGI Ref ID J:82371)
    • mutants exhibit increased stumbling frequency in a motor coordination test   (MGI Ref ID J:82371)
  • abnormal motor learning   (MGI Ref ID J:82371)
    • mice fail to improve performance in rotating rod trials in contrast to wild-type animals   (MGI Ref ID J:137190)
  • impaired balance
    • in a non-rotating rod test, equilibrium times of mutants are lower than controls in the first trial, but not the 2nd or 3rd trial   (MGI Ref ID J:82371)
  • impaired coordination
    • mutants exhibit poor performance in a motor training test with a rotarod, and are unable to improve their performance   (MGI Ref ID J:82371)
  • cellular phenotype
  • abnormal mitochondrion morphology
    • mitochondria lengths in Purkinje cells are 1.3-1.6 um, compared to lengths of 0.8-1.0 um in wild-type animals   (MGI Ref ID J:137190)
    • often, points where mitochondria become constricted are observed in mutants but not in wild-type mitochondria, suggestive of impaired fission   (MGI Ref ID J:137190)
  • nervous system phenotype
  • abnormal CNS synapse formation
    • mismatching between the length of the presynaptic active zones, in parallel fiber varicosities, and the postsynaptic differentiations in Purkinje cell dendritic spines   (MGI Ref ID J:82371)
  • abnormal Purkinje cell innervation
    • although most Purkinje cells are innervated by a single climbing fiber, about 19-20% of cells show innervation by more than one climbing fiber   (MGI Ref ID J:82371)
  • abnormal cerebellar cortex morphology
    • numerous clusters of naked spines are located in the molecular layer around secondary branches and spiny branchlets of Purkinje cell dendrites   (MGI Ref ID J:82371)
    • abnormal Purkinje cell dendrite morphology
      • Purkinje cell dendrites have ectopic spines, some of which are naked and bore very long postsynaptic differentiations directly apposed to astrocytic processes   (MGI Ref ID J:82371)
      • occasional appositions between a Purkinje cell dendritic spine and two parallel fiber varicosities   (MGI Ref ID J:82371)
    • abnormal cerebellar granule layer morphology
      • some granule cell pyknotic nuclei in the granule cell layer   (MGI Ref ID J:82371)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Grid2ho-4J related

Neurobiology Research
Ataxia (Movement) Defects
Cerebellar Defects
Receptor Defects
      glutamate receptor: ionotropic

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Grid2ho-4J
Allele Name hotfoot 4 Jackson
Allele Type Spontaneous
Strain of OriginDBA/2J
Gene Symbol and Name Grid2, glutamate receptor, ionotropic, delta 2
Chromosome 6
Gene Common Name(s) B230104L07Rik; GluD2; GluRdelta2; Lc; LcJ; RIKEN cDNA B230104L07 gene; cpr; creeper; ho; hotfoot; lurcher; lurcher Jackson; neuroscience mutagenesis facility, 408; nmf408; tapdancer; tpr;
Molecular Note Exons 5-8 are deleted which results in a 170 amino acid loss in the N-terminal LIVBP-like domain. [MGI Ref ID J:48151] [MGI Ref ID J:84411]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Draski LJ; Nash DJ; Gerhardt GA. 1994. CNS monoamine levels and motoric behaviors in the hotfoot ataxic mutant. Brain Res 645(1-2):69-77. [PubMed: 7914819]  [MGI Ref ID J:18056]

Lalouette A; Christians E; Guenet JL; Vriz S. 1997. Construction of a high-resolution genetic map encompassing the hotfoot locus. Mamm Genome 8(12):903-6. [PubMed: 9383282]  [MGI Ref ID J:44452]

Lalouette A; Lohof A; Sotelo C; Guenet J; Mariani J. 2001. Neurobiological effects of a null mutation depend on genetic context: comparison between two hotfoot alleles of the delta-2 ionotropic glutamate receptor. Neuroscience 105(2):443-55. [PubMed: 11672610]  [MGI Ref ID J:82371]

Grid2ho-4J related

Dickie MM. 1966. Hotfoot Mouse News Lett 34:30.  [MGI Ref ID J:13443]

Jones SM; Johnson KR; Yu H; Erway LC; Alagramam KN; Pollak N; Jones TA. 2005. A quantitative survey of gravity receptor function in mutant mouse strains. J Assoc Res Otolaryngol 6(4):297-310. [PubMed: 16235133]  [MGI Ref ID J:116914]

Kato AS; Knierman MD; Siuda ER; Isaac JT; Nisenbaum ES; Bredt DS. 2012. Glutamate receptor delta2 associates with metabotropic glutamate receptor 1 (mGluR1), protein kinase Cgamma, and canonical transient receptor potential 3 and regulates mGluR1-mediated synaptic transmission in cerebellar Purkinje neurons. J Neurosci 32(44):15296-308. [PubMed: 23115168]  [MGI Ref ID J:192293]

Lalonde R; Strazielle C. 2007. Spontaneous and induced mouse mutations with cerebellar dysfunctions: behavior and neurochemistry. Brain Res 1140:51-74. [PubMed: 16499884]  [MGI Ref ID J:120621]

Lalouette A; Christians E; Guenet JL; Vriz S. 1997. Construction of a high-resolution genetic map encompassing the hotfoot locus. Mamm Genome 8(12):903-6. [PubMed: 9383282]  [MGI Ref ID J:44452]

Lalouette A; Guenet JL; Vriz S. 1998. Hotfoot mouse mutations affect the delta2 glutamate receptor gene and are allelic to lurcher Genomics 50(1):9-13. [PubMed: 9628817]  [MGI Ref ID J:48151]

Lalouette A; Lohof A; Sotelo C; Guenet J; Mariani J. 2001. Neurobiological effects of a null mutation depend on genetic context: comparison between two hotfoot alleles of the delta-2 ionotropic glutamate receptor. Neuroscience 105(2):443-55. [PubMed: 11672610]  [MGI Ref ID J:82371]

Liu QA; Shio H. 2008. Mitochondrial morphogenesis, dendrite development, and synapse formation in cerebellum require both Bcl-w and the glutamate receptor delta2. PLoS Genet 4(6):e1000097. [PubMed: 18551174]  [MGI Ref ID J:137190]

Lutz C; Frankel W. 1994. Remutation - Grid2<ho-8J> Mouse Genome 92(2):355.  [MGI Ref ID J:155927]

Matsuda S; Yuzaki M. 2002. Mutation in hotfoot-4J mice results in retention of delta2 glutamate receptors in ER. Eur J Neurosci 16(8):1507-16. [PubMed: 12405964]  [MGI Ref ID J:107991]

The Jackson Laboratory Office of Genetic Resources. 1975. Registry of Remutations at The Jackson Laboratory MGI Direct Data Submission :.  [MGI Ref ID J:155928]

Wang Y; Matsuda S; Drews V; Torashima T; Meisler MH; Yuzaki M. 2003. A hot spot for hotfoot mutations in the gene encoding the delta2 glutamate receptor. Eur J Neurosci 17(8):1581-90. [PubMed: 12752376]  [MGI Ref ID J:84411]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryFemales homozygous for Grid2ho-4J tend to be poor breeders. Males homozygous for Grid2ho-4J are fertile although males of the original Grid2ho mutation are sterile.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Embryos

Price (US dollars $)
Frozen Embryo $1600.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Embryos

Price (US dollars $)
Frozen Embryo $2080.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

General Supply Notes

  • View the complete collection of spontaneous mutants in the Mouse Mutant Resource.

Control Information

  Control
   Heterozygote from the colony
   000671 DBA/2J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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