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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6EiC3-+ a/HoxdUl A/J (Changed: 15-DEC-04 ) Type Mutant Stock; Spontaneous Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N14p
Generation DefinitionsAppearance
agouti, unaffected
Related Genotype: A +/? +
agouti, limb abnormality
Related Genotype: A LnpUl/? +
black, unaffected
Related Genotype: a +/a +
black, limb abnormality
Related Genotype: a LnpUl/a +Development
The ulnaless mutation was identified in the progeny of an HT linkage testing stock female (homozygous for a, Gdf5bp Sqk3fz Mlphln Pldnpa and Ap3b1pe) bred with a (C3H/HeJ x 101/H)F1 male that had received two 500 rad doses of X-rays 24 hours apart. This mutation was then maintained by breeding a heterozygote to a (C3H/HeJ x 101/H)F1, and was imported from Harwell into The Jackson Laboratory by Dr. Eva Eicher after 1968 and prior to 1970. At some point in the 1970?s it was maintained by breeding female heterozygotes to (C57BL/6J x CsH/HeJ)F1 males, such that in 1986 it reached generation N20. In 1984 embryos were generated for cryopreservation by breeding female heterozygotes at generation N13 with C57BL/6JEi males.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying A allele
003301 (C57BL/6J x C3H-Eya1bor)F1/J 002083 B6 x B6EiC3 a/A-T(7;16)235Dn/J 000507 B6 x B6EiC3 a/A-Otcspf/J 000628 B6.CE-A Amy1b Amy2a5b/J 004200 B6;CBACa Aw-J/A-Npr2cn-2J/GrsrJ 000604 B6C3 a/A-T(10;13)199H +/+ Lystbg-J/J or Lystbg-2J/J 001752 B6CBCa Aw-J/A-T(7;15)9H/J 006450 B6EiC3 a/A-Vss/GrsrJ 000504 B6EiC3Sn a/A-Cacnb4lh/J 000553 B6EiC3Sn a/A-Egfrwa2 Wnt3avt/J 001811 B6EiC3Sn a/A-Otcspf-ash/J 002343 B6EiC3Sn a/A-Otcspf/J 001923 B6EiC3Sn a/A-Ts(417)2Lws TimT(4;17)3Lws/J 000200 C3FeB6 A/Aw-J-Ankank/J 000638 C3FeB6 A/Aw-J-Spnb4qv-J/J 000283 LT.CAST-A/J 001759 STOCK A Tyrc Sha/J View Strains carrying A (17 strains)
Phenotype
Phenotype Information
assigned by genotype
LnpUl/Lnp+
B6EiC3-+ a/LnpUl A/J
- homeostasis/metabolism phenotype
- increased bleeding time
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
LnpUl/Lnp+
involves: 101/H * C3H/HeJ
- limbs/digits/tail phenotype
- abnormal fibula morphology
- deformities (MGI Ref ID J:13461)
- abnormal tibia morphology
- deformities (MGI Ref ID J:13461)
- short radius
- extreme reduction (MGI Ref ID J:13461)
- short ulna
- extreme reduction (MGI Ref ID J:13461)
- skeleton phenotype
- abnormal fibula morphology
- deformities (MGI Ref ID J:13461)
- abnormal tibia morphology
- deformities (MGI Ref ID J:13461)
- short radius
- extreme reduction (MGI Ref ID J:13461)
- short ulna
- extreme reduction (MGI Ref ID J:13461)
- reproductive system phenotype
- male infertility
- males do not breed (MGI Ref ID J:13461)
LnpUl/Lnp+
involves: 101/H * C3H/HeJ * C57BL/6J
- limbs/digits/tail phenotype
- abnormal fibula morphology
- short fibula (MGI Ref ID J:10491)
- abnormal metacarpal bone morphology
- articulations to distal ends of long bones are abnormal (MGI Ref ID J:10491)
- abnormal metatarsal bone morphology
- articulations to distal ends of long bones are abnormal (MGI Ref ID J:10491)
- abnormal tibia morphology
- rotated 90 degrees (MGI Ref ID J:10491)
- bowed tibia (MGI Ref ID J:10491)
- short radius
- extreme reduction (MGI Ref ID J:10491)
- short ulna
- extreme reduction (MGI Ref ID J:10491)
- skeleton phenotype
- abnormal fibula morphology
- short fibula (MGI Ref ID J:10491)
- abnormal metacarpal bone morphology
- articulations to distal ends of long bones are abnormal (MGI Ref ID J:10491)
- abnormal metatarsal bone morphology
- articulations to distal ends of long bones are abnormal (MGI Ref ID J:10491)
- abnormal tibia morphology
- rotated 90 degrees (MGI Ref ID J:10491)
- bowed tibia (MGI Ref ID J:10491)
- short radius
- extreme reduction (MGI Ref ID J:10491)
- short ulna
- extreme reduction (MGI Ref ID J:10491)
- reproductive system phenotype
- male infertility
LnpUl/Lnp+
involves: C3H/HeJ * C57BL/6J * FVB/N
- lethality-postnatal
- postnatal lethality
- at weaning there are fewer heterozygotes, both male and female, than expeted from heterozygote x FVB/N matings on a predominantly FVB/N background, but not on a predominantly MOLF/Ei background (MGI Ref ID J:37225)
LnpUl/LnpUl
involves: C3H/HeJ * C57BL/6J * FVB/N
- limbs/digits/tail phenotype
- abnormal limb morphology
- homozygotes have a more severe reduction of the ulna and radius than heterozygotes and there is a severe loss of the fibula and severe reduction and bowing of the tibia (MGI Ref ID J:37225)
- bowed tibia (MGI Ref ID J:37225)
- short fibula (MGI Ref ID J:37225)
- short radius (MGI Ref ID J:37225)
- short tibia (MGI Ref ID J:37225)
- short ulna (MGI Ref ID J:37225)
- skeleton phenotype
- bowed tibia (MGI Ref ID J:37225)
- delayed bone ossification
- the ossification centers of the ulna and fibula are absent in homozygous neonates (MGI Ref ID J:37225)
- short fibula (MGI Ref ID J:37225)
- short radius (MGI Ref ID J:37225)
- short tibia (MGI Ref ID J:37225)
- short ulna (MGI Ref ID J:37225)
- lethality-postnatal
- postnatal lethality
- although the anticipated 1:2:1 Mendelian ratio is obtained at birth from heterozygous intercrosses, very few homozygotes are found at wean age on this predominantly FVB/N background, and those that do survive are male, but this loss of homozygotes is not gound on a predominantly MOLF/Ei background (MGI Ref ID J:37225)
LnpUl/LnpUl
involves: C3H/HeJ * C57BL/6J * MOLF/Ei
- limbs/digits/tail phenotype
- abnormal limb morphology
- homozygotes have a more severe reduction of the ulna and radius than heterozygotes and there is a severe loss of the fibula and severe reduction and bowing of the tibia (MGI Ref ID J:37225)
- bowed tibia (MGI Ref ID J:37225)
- short fibula (MGI Ref ID J:37225)
- short radius (MGI Ref ID J:37225)
- short tibia (MGI Ref ID J:37225)
- short ulna (MGI Ref ID J:37225)
- skeleton phenotype
- bowed tibia (MGI Ref ID J:37225)
- delayed bone ossification
- the ossification centers of the ulna and fibula are absent in homozygous neonates (MGI Ref ID J:37225)
- short fibula (MGI Ref ID J:37225)
- short radius (MGI Ref ID J:37225)
- short tibia (MGI Ref ID J:37225)
- short ulna (MGI Ref ID J:37225)
This mouse can be used to support research in many areas including:LnpUl related
Developmental Biology Research
Skeletal Defects
Genes & Alleles
Gene & Allele Information
| Allele Symbol | A | ||
|---|---|---|---|
| Allele Name | wild type agouti | ||
| Allele Type | Spontaneous | ||
| Common Name(s) | dark-bellied agouti; | ||
| Strain of Origin | various | ||
| Gene Symbol and Name | a, nonagouti | ||
| Chromosome | 2 | ||
| Gene Common Name(s) | AGSW; AGTI; AGTIL; ASP; As; MGC126092; MGC126093; SHEP9; agouti; agouti signal protein; agouti suppressor; | ||
| General Note | The A allele is usually regarded as a wild-type allele. Hairs are black with a subapical yellow band. This black-yellow-black pattern is referred to as agouti. The general appearance is yellowish brown, slightly lighter on the belly than on the back. | ||
| Molecular Note | This allele, often referred to as wild-type, comprises a novel 131 amino acid protein encoded in a gene comprising four exons, three coding, spanning 18kb. Unique changes in this gene account for all other alleles that have been molecularly characterized. The expression of this allele is almost always dominant to other alleles of this gene. [MGI Ref ID J:3523] | ||
| Allele Symbol | LnpUl | ||
| Allele Name | ulnaless | ||
| Allele Type | Radiation induced | ||
| Common Name(s) | Ul; | ||
| Strain of Origin | (C3H/HeJ x 101/H)F1 | ||
| Gene Symbol and Name | Lnp, limb and neural patterns | ||
| Chromosome | 2 | ||
| Gene Common Name(s) | 2310011O18Rik; 4921514L11Rik; 9530051D01Rik; AI666268; DKFZp686G1442; MGC102862; RIKEN cDNA 2310011O18 gene; RIKEN cDNA 4921514L11 gene; RIKEN cDNA 9530051D01 gene; Ul; expressed sequence AI666268; lunapark; ulnaless; | ||
| Molecular Note | The ulnaless mouse has been characterized as a paracentric inversion with a centromeric breakpoint into the lunapark gene and a telomeric breakpoint 770 kb away. This inversion disrupts the lunapark gene and increases the distance between the neighboringHoxD cluster and a cluster of enhancer sequences controlling several distinct genes over a large region. Genes Evx2, Hoxd12 and Hoxd13 are all misexpressed in the limb buds and genital buds of the ulnaless mouse. The inverted DNA includes Evx2, the HoxD complex, Mtx2, as well as some pseudogenes. [MGI Ref ID J:83372] | ||
| Allele Symbol | a | ||
| Allele Name | nonagouti | ||
| Allele Type | Spontaneous | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.Helpful Links
Genotyping resources and troubleshooting
References
References
Additional References
Spitz F; Gonzalez F; Duboule D. 2003. A global control region defines a chromosomal regulatory landscape containing the HoxD cluster. Cell 113(3):405-17. [PubMed: 12732147] [MGI Ref ID J:83372]
A relatedLnpUl relatedBlewitt ME; Vickaryous NK; Hemley SJ; Ashe A; Bruxner TJ; Preis JI; Arkell R; Whitelaw E. 2005. An N-ethyl-N-nitrosourea screen for genes involved in variegation in the mouse. Proc Natl Acad Sci U S A 102(21):7629-34. [PubMed: 15890782] [MGI Ref ID J:99816]
Bultman SJ; Michaud EJ; Woychik RP. 1992. Molecular characterization of the mouse agouti locus. Cell 71(7):1195-204. [PubMed: 1473152] [MGI Ref ID J:3523]
Bundschuh VG; Madry M. 1988. [atwp mutation in an albino mouse substrain (AB/Hum-1)] Z Versuchstierkd 31(6):249-54. [PubMed: 3227730] [MGI Ref ID J:16568]
Czyzyk TA; Sikorski MA; Yang L; McKnight GS. 2008. Disruption of the RIIbeta subunit of PKA reverses the obesity syndrome of Agouti lethal yellow mice. Proc Natl Acad Sci U S A 105(1):276-81. [PubMed: 18172198] [MGI Ref ID J:131039]
Dickie MM. 1969. Mutations at the agouti locus in the mouse. J Hered 60(1):20-5. [PubMed: 5798139] [MGI Ref ID J:30922]
Dry FW. 1928. The agouti coloration of the mouse (Mus Musculus) and the rat (Mus Norvegicus). J Genet 20:131-144. [MGI Ref ID J:46318]
Galbraith DB; Wolff GL; Brewer NL. 1979. Tissue microenvironment and the genetic control of hair pigment patterns in mice Dev Genet 1(2):167-179. [MGI Ref ID J:156092]
Guido V, and The Mouse Mutant Resource (MMR) at The Jackson Laboratory. 2002. Two new mutations of white bellied agouti, w-46J and w-47J MGI Direct Data Submission :. [MGI Ref ID J:77218]
Jackson IJ; Budd PS; Keighren M; McKie L. 2007. Humanized MC1R transgenic mice reveal human specific receptor function. Hum Mol Genet 16(19):2341-8. [PubMed: 17652101] [MGI Ref ID J:129904]
Mather K; North SB. 1940. Umbrous: a case of dominance modification in mice. J Genet 40:229-41. [MGI Ref ID J:280]
Perry WL; Copeland NG; Jenkins NA. 1994. The molecular basis for dominant yellow agouti coat color mutations. Bioessays 16(10):705-7. [PubMed: 7980472] [MGI Ref ID J:21244]
Phillips RJS. 1966. A cis-trans position effect at the A locus of the house mouse. Genetics 54(2):485-95. [PubMed: 5968639] [MGI Ref ID J:5027]
Quevedo WC Jr.; Chase HB. 1958. An analysis of the light mutation of coat color in mice. J Morphol 102:329-345. [MGI Ref ID J:13094]
Silvers WK. 1979. The Coat Colors of Mice; A Model for Mammalian Gene Action and Interaction. In: The Coat Colors of Mice. Springer-Verlag, New York. [MGI Ref ID J:78801]
Davisson MT; Cattanach BM. 1990. The mouse mutation ulnaless on chromosome 2. J Hered 81(2):151-3. [PubMed: 2338491] [MGI Ref ID J:10491]
Herault Y; Fraudeau N; Zakany J; Duboule D. 1997. Ulnaless (Ul), a regulatory mutation inducing both loss-of-function and gain-of-function of posterior Hoxd genes. Development 124(18):3493-500. [PubMed: 9342042] [MGI Ref ID J:43310]
Morris T. 1967. Ul. Mouse News Lett 36:34. [MGI Ref ID J:13461]
Peichel CL; Abbott CM; Vogt TF. 1996. Genetic and physical mapping of the mouse Ulnaless locus. Genetics 144(4):1757-67. [PubMed: 8978061] [MGI Ref ID J:37225]
Peichel CL; Prabhakaran B; Vogt TF. 1997. The mouse Ulnaless mutation deregulates posterior HoxD gene expression and alters appendicular patterning. Development 124(18):3481-92. [PubMed: 9342041] [MGI Ref ID J:43311]
Rusiniak ME; O'Brien EP; Novak EK; Barone SM; McGarry MP; Reddington M; Swank RT. 1996. Molecular markers near the mouse brachymorphic (bm) gene, which affects connective tissues and bleeding time. Mamm Genome 7(2):98-102. [PubMed: 8835524] [MGI Ref ID J:31800]
Spitz F; Gonzalez F; Duboule D. 2003. A global control region defines a chromosomal regulatory landscape containing the HoxD cluster. Cell 113(3):405-17. [PubMed: 12732147] [MGI Ref ID J:83372]
Health & husbandry
Health & Colony Maintenance Information
Currently there is no information available for this strain. This may be due to the supply level of this strain.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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 |
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
|
|
|
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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