Strain Name: |
JIGR/DnJ |
|---|---|
Stock Number: |
000572 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
Jittery and Grizzled (Changed: 15-DEC-04
) |
| Genes & Alleles | Atcay; Atcayji; gr; |
Product Information
Strain Details
Type Inbred Strain Additional information on Inbred Strains. Type JAX® GEMM® Strain - Spontaneous Mutation Additional information on JAX® GEMM® Strains. Species laboratory mouse Generation F75 Appearance
agouti, unaffected
Related Genotype: A/A ? +/+ ? (unproven) or A/A gr +/+ Atcayji (proven)Important Note
This strain carries gr and Atcayji in repulsion.Strain Description
JIGR/Dn is maintained with jittery (Atcayji) and grizzled (gr) in repulsion.Atcayji homozygotes can first be identified at approximately 12 days of age by a leaning, zig-zag gait when they attempt to run and by the difficulty they have righting themselves when placed on their backs. Disease progression is rapid such that within two more days mutants are found to crouch on their heels in a squatting position and can not run without falling. Within three to four days of the first symptoms, tetany is seen during exertion or excitement. This is initially most pronounced in the forelegs, which the mice beat up and down during these two- to three-second spasms. Failure to gain weight is seen by the third week of life and it is unclear whether this results from an inability to take in food. The severity and frequency of tetany increases, and during the fourth week weight loss and increased weakness precede death. The mean age of death is approximately 31 days. Tetrahydrobiopterin levels in the brain and GTP cyclohydrolase activity in the liver are lower in Atcayji homozygotes than normal. At 20 days of age, serum calcium is within normal ranges. Parabiotic twins made with an affected and an unaffected sibling do not transfer a mutant phenotype to the normal partner indicating that a diffusible factor does not cause the mutant phenotype. However, tetany and weight loss are inhibited in the mutant partner which also has increased longevity, yet still develops failed muscle coordination and a crouching posture. (DeOme, 1945; Snell, 1950; Duch et al., 1986.)
gr is a recessive mutation that occasionally causes tail kinks and consistently causes dilution of the yellow pigment but not the black pigment of the hair. The coat color has been described as similar to chinchilla (Tyrc-ch/Tyrc-ch) but with the black pigment remaining undiluted. On the agouti JIGR/Dn background the gr/gr coat color is grayish agouti. On a non-agouti background the hair in the ears and around the genitalia is white. The gr mutation causes 40-50% mortality prior to phenotypic classification and this affects males more than females. This mortality is both postnatal and prenatal from approximately 10 days onward. Pregnant dams expected to carry some homozygotes have been found to carry some dead embryos some of which had craneofacial abnormalities including shortened snout and swollen optic and occipital regions. At birth homozygotes weigh an average of one quarter less than their wildtype siblings. Although they increase in weight as suckling pups, as adults they still weigh 5-25% less than their wild type siblings. (Falconer, 1950; Bloom and Falconer, 1966.)
Strain Development
Jittery (Atcayji) arose as a spontaneous mutation in BALB/c, the Bagg albino strain, before 1945. It was segregating in the waltzer stock (see V/Le, Stock No. 000275) that Dr. Ludwin sent to Dr. Snell at the Jackson Laboratory in 1947. Jittery remained segregating in the waltzer stock until 1959 when a jittery (Atcayji/Atcayji) male was outcrossed to a C57BL10 female. Following this cross sibling matings were used until F15 in 1964 when a Atcayji/Atcayji male was mated to an inbred grizzled (gr/gr) female at F30. Grizzled had appeared in descendants of mice from a cross of strain A at Edinburgh and a fuzzy stock from Cambridge. The Jackson Laboratory received grizzled from Dr. D. Falconer at Edinburgh in 1950. It had been crossed twice to CBA and was then sister x brother bred by Snell using forced heterozygosis. Because Atcayji and gr were closely linked on Chromosome 10 a balanced cross with the two loci in repulsion was used to maintain the JIGR strain. It was inbred and cryopreserved in 1987 by mating presumed Atcayji +/+ gr males and females at F74.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Atcayji | ||
|---|---|---|---|
| Allele Name | jittery | ||
| Common Name(s) | ji; | ||
| Strain of Origin | BALB/c | ||
| Gene Symbol and Name | Atcay, ataxia, cerebellar, Cayman type homolog (human) | ||
| Chromosome | 10 | ||
| Gene Common Name(s) | 3322401A10Rik; BB077577; BNIP-H; CLAC; KIAA1872; RIKEN cDNA 3322401A10 gene; expressed sequence BB077577; hes; hesitant; ji; jittery; | ||
| Molecular Note | The mutation in the jittery mouse has been identified as a B1 element insertion in exon 4 and results in a predicted protein of only 62 endogenous residues plus 21 missense residues. [MGI Ref ID J:85793] | ||
| Allele Symbol | gr | ||
| Allele Name | grizzled | ||
| Strain of Origin | A x STOCK-Sgk3fz | ||
| Molecular Note | This allele maps to a region of homology with the human paralemmin gene, PALM. Direct sequencing of RNA from brains of homozygous mice and analysis of the paralemmin coding region showed no sequence abnormalities. [MGI Ref ID J:47933] | ||
Control Information
| Allele | Control | |
|---|---|---|
| Atcayji | Untyped from the colony | |
| gr | Untyped from the colony | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Atcayji allele
008140 B6.C(Cg)-Atcayji/BurJ View Strains carrying Atcayji (1 strain)
000279 STOCK gr +/+ Ap3d1mh/J
001904 C3H-Atcayji-hes/J
Research Applications
This mouse can be used to support research in many areas including:Atcayji related
gr relatedDevelopmental Biology Research
Growth Defects
Postnatal Mortality (Homozygous)
Mouse/Human Gene Homologs
ataxia, cerebellar, Cayman type
Neurobiology Research
Ataxia (Movement) Defects
Epilepsy
Tremor Defects
Dermatology Research
Color and White Spotting Defects
Developmental Biology Research
Craniofacial and Palate Defects
Growth Defects
Perinatal Lethality (Homozygous)
Postnatal Mortality
References
Additional ReferencesPrice and Supply Information
| Strain Name: | JIGR/DnJ |
| Stock Number: | 000572 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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