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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Strain; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Development
The furless mutation (Ctslfs) appeared in an unpedigreed stock at Ohio State University in the early 1950s. It was brought to The Jackson Laboratory by Dr. E. L. Green in 1956. The stock was maintained by sibling matings with forced heterozygosis. The stock was called FSB. It was cryopreserved in 1984 by mating homozygous males, produced from an outcross to C57BL/6J and crossed back to the FSB stock, to C57BL/6J females.
Related Strains
Strains carrying a allele
View Strains carrying a (104 strains)
008352 NOD.129P2(B6)-Ctsltm1Cptr/RclJ
Phenotype
Phenotype Information
assigned by genotype
Ctslfs/Ctslfs
involves: C57BL/6 * FSB/GnEi
- endocrine/exocrine gland phenotype
- abnormal seminiferous tubule morphology (MGI Ref ID J:81950)
- 18% of tubules were atrophic, corresponding with a 12 fold increase in atrophy
- decreased testis weight (MGI Ref ID J:81950)
- although males are fertile, a 25% weight reduction was observed in mice at 110 and 120 days of age relative to age matched control mice
- reproductive system phenotype
- abnormal seminiferous tubule morphology (MGI Ref ID J:81950)
- 18% of tubules were atrophic, corresponding with a 12 fold increase in atrophy
- abnormal spermatogenesis (MGI Ref ID J:81950)
- 16% reduction in formation of preloptotene spermatocytes in non-atrophic tubules
- 25% reduction of complete differentiation of preloptotene spermatocytes into pachytene spermatocytes in non-atrophic tubules
- decreased testis weight (MGI Ref ID J:81950)
- although males are fertile, a 25% weight reduction was observed in mice at 110 and 120 days of age relative to age matched control mice
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Ctslfs/Ctslfs
involves: C57BL/6 * unpedigreed stock
- lethality-postnatal
- postnatal lethality (MGI Ref ID J:5217)
- incomplete penetrance; viability up to weaning age of furless mice is considerably reduced, but survivors are fertile; heterozygotes have normal reproductive fitness and radiation sensitivity
- skin/coat/nails phenotype
- alopecia (MGI Ref ID J:15332)
- age of onset is 19 days, beginning at the head and progressing toward the tail region of the back
- a new coat starts to grow at age 28 - 30 days
- mature homozygous mice are always partially devoid of hair
- touch/vibrissae phenotype
- absent vibrissae (MGI Ref ID J:15332)
- vibrissae fail to penetrate the epidermis at 2 days after birth
This mouse can be used to support research in many areas including:Ctslfs related
Dermatology Research
Skin and Hair Texture Defects
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Ctslfs | ||
|---|---|---|---|
| Allele Name | furless | ||
| Allele Type | Spontaneous | ||
| Strain of Origin | unpedigreed stock | ||
| Gene Symbol and Name | Ctsl, cathepsin L | ||
| Chromosome | 13 | ||
| Gene Common Name(s) | 1190035F06Rik; CATHL; CATL; Cat L; FLJ31037; MEP; RIKEN cDNA 1190035F06 gene; fs; furless; major excreted protein; nackt; nkt; | ||
| General Note | The phenotype of homozygous furless mice was notably similar to the phenotype observed in Ctsltm1Cptr homozygous mice. An allele test between the two mutant strains of mice was performed, where they failed to complement, indicating the two mutants are allelic. | ||
| Molecular Note | The mutation in the furless mutant was identified as a G to A transition in exon 5, resulting in an arginine to glycine substitution at position 149 of the protein. [MGI Ref ID J:70118] | ||
| Allele Symbol | a | ||
| Allele Name | nonagouti | ||
| Allele Type | Spontaneous | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Roth W; Deussing J; Botchkarev VA; Pauly-Evers M; Saftig P; Hafner A; Schmidt P; Schmahl W; Scherer J; Anton-Lamprecht I; Von Figura K; Paus R; Peters C. 2000. Cathepsin L deficiency as molecular defect of furless: hyperproliferation of keratinocytes and pertubation of hair follicle cycling. FASEB J 14(13):2075-86. [PubMed: 11023992] [MGI Ref ID J:70118]
Additional References
Ctslfs relatedBoudreau F; Lussier CR; Mongrain S; Darsigny M; Drouin JL; Doyon G; Suh ER; Beaulieu JF; Rivard N; Perreault N. 2007. Loss of cathepsin L activity promotes claudin-1 overexpression and intestinal neoplasia. FASEB J 21(14):3853-65. [PubMed: 17622569] [MGI Ref ID J:134918]
Green EL. 1971. Fitness of heterozygotes of deleterious recessive mutations in the mouse. Mutat Res 12(3):281-9. [PubMed: 5563943] [MGI Ref ID J:5217]
Green EL. 1954. The genetics of a new hair deficiency, furless, in the house mouse J Hered 45:115-18. [MGI Ref ID J:15332]
Sweet HO. 1987. Acromelanic (c<a>) Mouse News Lett 78:56. [MGI Ref ID J:14994]
Wright WW; Smith L; Kerr C; Charron M. 2003. Mice that express enzymatically inactive cathepsin L exhibit abnormal spermatogenesis. Biol Reprod 68(2):680-7. [PubMed: 12533435] [MGI Ref ID J:81950]
Health & husbandry
Health & Colony Maintenance Information
Currently there no information available for this strain. This may be due to the supply level of this strain.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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