Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Spontaneous Mutation; Additional information on Genetically Engineered and Mutant Mice. Type Inbred Strain; Additional information on Inbred Strains. Visit our online Nomenclature tutorial. Species laboratory mouse Generation F62

Important Note
This strain carries Re and shm in repulsion.

Control Information

	Control
	Untyped from the colony
Considerations for Choosing Controls

Related Strains

Strains carrying   Krt25^Re allele

000568	B6.Cg-Pmp22Tr-J Krt25Re/+ +/J
000126	B6By.Cg-Sd Mcoln3Va-J Krt25Re/J
000268	RSV/LeJ

View Strains carrying   Krt25^Re     (3 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Krt25^Re/Krt25^Re

        B6.Cg-Pmp22^Tr-J Krt25^Re/+ +/J

• skin/coat/nails phenotype
• abnormal hair follicle morphology (MGI Ref ID J:130100)
  • at 1 month, hair follicles are bent and shorter than in wild-type mice
• abnormal hair shaft morphology (MGI Ref ID J:130100)
  • at 1 month, hair shafts are fragile
• enlarged sebaceous gland (MGI Ref ID J:130100)
• waved hair (MGI Ref ID J:130100)
  • beginning at 1 month of age pelage is wavy compared to wild-type mice but this waviness becomes weaker as mice age
• touch/vibrissae phenotype
• curly vibrissae (MGI Ref ID J:130100)
  • beginning at 1 month of age, whiskers are irregular and fragile
• growth/size phenotype
• decreased body size (MGI Ref ID J:130100)
• endocrine/exocrine gland phenotype
• enlarged sebaceous gland (MGI Ref ID J:130100)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Krt25^Re related

Dermatology Research
Skin and Hair Texture Defects

shm related

Neurobiology Research
Ataxia (Movement) Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Krt25Re
Allele Name		rex
Allele Type		Spontaneous
Common Name(s)		Re;
Strain of Origin		Outbred
Gene Symbol and Name		Krt25, keratin 25
Chromosome		11
Gene Common Name(s)		4631426H08Rik; KRT25A; Ka38; RIKEN cDNA 4631426H08 gene; mIRSa1;
General Note		A mutation in the gasdermin 3 locus (Gsdm3) has been described for the Reden allele. However, no mutations in gasdermin 3 could be found associated with the Re allele. Therefore, the Re marker has not been merged with Gsdm3. (J:93938) The original rex mutation was first described by Crew and Auerbach (J:15328), who obtained it from a commercial breeder. Both homozygotes and heterozygotes are fully viable and fertile. Homozygotes have a slightly more extreme expression than heterozygotes when young (J:224). Both have curly whiskers and wavy coats. The waviness of the coat disappears in adults, but the vibrissae and guard hairs remain curly. In both homozygotes and heterozygotes, all hair types are present, but the shaft diameter is irregular with variation in width of the cortex, and with some bends and twists. The hair defects appear to be due to a defect in the internal root sheath which is irregular in shape and unable to support the hair shaft and control its diameter (J:15247).
Molecular Note		This allele contains a nucleotide substitution that results in an amino acid substitution of proline for leucine at position 381 (L381P). [MGI Ref ID J:130100]
Allele Symbol		shm
Allele Name		shambling
Allele Type		Radiation induced
Strain of Origin		random-bred
Gene Symbol and Name		shm, shambling
Chromosome		11
General Note		This mutation was found in a randombred population which had been exposed to X-irradiation in the four preceding generations. Homozygotes are smaller than normal and seldom fertile. They are first recognizable at 16 to 18 days by their small size and wobbly gait. Shambling mice walk with the hindlimbs held relatively stiff and take exaggerated steps so that a hindfoot often lands in front of the forefeet. They do not swim well, and when taken out of the water may undergo a spell of violent trembling lasting half an hour or more (J:5040). Viability and longevity are somewhat reduced (J:5217). In the central nervous system and in a number of visceral organs there are reported to be focal deposits of phospholipid-like material which has not been identified (J:5052). There are no degenerative myelin changes, and brain gangliosides are normal in composition and amount (J:5683). In brain microsomes, there is a 60% decrease in rate of synthesis of phosphatidylserine (PS) in homozygotes and an 80% decrease in heterozygotes, the loss probably being due to loss of the calmodulin- sensitive PS synthesis present in normal brain microsomes (J:7584).

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Additional References

Green EL. 1967. Shambling, a neurological mutant of the mouse. J Hered 58(2):65-8. [PubMed: 6036114]  [MGI Ref ID J:5040]

Meier H. 1967. Pathological findings in shambling, a hereditary neuropathy of mice. J Neuropathol Exp Neurol 26(4):620-33. [PubMed: 6053733]  [MGI Ref ID J:5052]

Krt25^Re related

CARTER TC; PHILLIPS RJ. 1953. The sex distribution of waved-2, shaker-2 and Rex in the house mouse. Z Indukt Abstamm Vererbungsl 85(4):564-78. [PubMed: 13170359]  [MGI Ref ID J:225]

Carter TC. 1951. Wavy-coated mice: phenotypic interactions and linkage tests between rex and (a) waved-1, (b) waved-2 J Genet 50:268-76.  [MGI Ref ID J:224]

Crew FAE; Auerbach C. 1939. Rex: a dominant autosomal monogenic coat texture character in the mouse J Genet 38:341-44.  [MGI Ref ID J:15328]

Hogan ME; King LE Jr; Sundberg JP. 1995. Defects of pelage hairs in 20 mouse mutations. J Invest Dermatol 104(5 Suppl):31S-32S. [PubMed: 7738386]  [MGI Ref ID J:25255]

Sundberg JP (ed.). 1994. . In: Handbook of Mouse Mutations with Skin and Hair Abnormalities: Animal Models and Biomedical Tools. CRC Press, Boca Raton.  [MGI Ref ID J:30359]

Tanaka S; Miura I; Yoshiki A; Kato Y; Yokoyama H; Shinogi A; Masuya H; Wakana S; Tamura M; Shiroishi T. 2007. Mutations in the helix termination motif of mouse type I IRS keratin genes impair the assembly of keratin intermediate filament. Genomics 90(6):703-11. [PubMed: 17920809]  [MGI Ref ID J:130100]

Trigg MJ. 1972. Hair growth in mouse mutants affecting coat texture. J Zool 168:165-198.  [MGI Ref ID J:15247]

shm related

Green EL. 1971. Fitness of heterozygotes of deleterious recessive mutations in the mouse. Mutat Res 12(3):281-9. [PubMed: 5563943]  [MGI Ref ID J:5217]

Green EL. 1967. Shambling, a neurological mutant of the mouse. J Hered 58(2):65-8. [PubMed: 6036114]  [MGI Ref ID J:5040]

Meier H. 1967. Pathological findings in shambling, a hereditary neuropathy of mice. J Neuropathol Exp Neurol 26(4):620-33. [PubMed: 6053733]  [MGI Ref ID J:5052]

Seyfried TN; Weber EJ; Daniel WL. 1976. Absence of brain ganglioside abnormalities in shambling mutant mice. J Neurochem 27(1):295-6. [PubMed: 956836]  [MGI Ref ID J:5683]

Yamaguchi T; Matsumura Y; Yamaguchi M. 1984. Decrease in phosphatidylserine synthesis in brain microsomes of Shambling mutant mouse. Biochem Int 8(3):347-52. [PubMed: 6433935]  [MGI Ref ID J:7584]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply	Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes	Cryorecovery - Standard. At least two mice that carry the mutation (if it is a mutant strain) will be provided. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotypes and genders are needed. IMPORTANT NOTE: The genotypes of the animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire for possible genotypes for this specific strain. Animals typically ship within 13 to 16 weeks from your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will typically ship within 25 weeks. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. Genomic DNA is available for this strain from the Mouse DNA Resource.
Important Note	This strain carries Re and shm in repulsion.

Control Information

	Control
	Untyped from the colony
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.