Strain Name: |
C3H/HeOuJ |
|---|---|
Stock Number: |
000635 |
Availability: | Level 2 |
Price and Supply Information | |
General Terms and Conditions |
| Strain Common Names | C3Ou; C3H Outzen; |
| Genes & Alleles | Pde6b; Pde6brd1; |
Product Information
Strain Details
Type Inbred Strain Additional information on Inbred Strains. Mating System Sibling x Sibling (Female x Male) Species laboratory mouse H2 Haplotype k Generation F184 (03-JAN-08) Appearance
agouti
Related Genotype: A/AImportant Note
This strain does not carry mouse mammary tumor virus (MMTV). See JAX Notes, May 2000, #480. This strain is also homozygous for the retinal degeneration allele Pde6brd1. See article "Genetic Background Effects: Can Your Mice See?", JAX Notes Spring 2002, No. 485.Strain Description
C3H/HeOuJ mice are used as a general purpose strain in a wide variety of research areas including cancer and sensorineural, research. C3H/HeOuJ mice and all other C3H substrains at The Jackson Laboratory are homozygous for the retinal degeneration 1 mutation (Pde6brd1), causing blindness by weaning age. There is also a high incidence of hepatomas in C3H mice (reportedly 72-91% in males at 14 months, 59% in virgin females, 30-38% in breeding females). Despite the lack of exogenous mouse mammary tumor virus (MMTV), virgin and breeding females may still develop some mammary tumors later in life.Strain Development
The C3H parent strain was developed by LC Strong in 1920 from a cross of a Bagg albino female with a DBA male followed by selection for high incidence of mammary tumors. This high incidence resulted from exogenous mouse mammary tumor virus (MMTV) transmitted through the mother's milk. The Jackson Laboratory maintains four C3H substrains, C3H/HeJ (Stock No. 000659), C3H/HeOuJ (Stock No. 000635), C3HeB/FeJ (Stock No. 000658) and C3H/HeSnJ (Stock No. 000661) that are now free of exogenous MMTV. C3H/HeJ and C3H/HeOuJ mice previously carried MMTV but were rederived in 1999 during planned efforts to increase the overall health status of the mice and the virus not reintroduced. C3H/HeJ and C3H/HeOuJ substrains were separated in 1952 and are genetically very similar. However, a spontaneous mutation occurred in C3H/HeJ sometime between 1960 and 1968 at lipopolysaccharide response locus (mutation in toll-like receptor 4 gene, Tlr4lps) making C3H/HeJ mice endotoxin resistant while the other three C3H strains are endotoxin sensitive.
Gene & Allele Details
| Allele Symbol | Pde6brd1 | ||
|---|---|---|---|
| Allele Name | retinal degeneration 1 | ||
| Common Name(s) | rd; rd-1; rd1; rodless retina; | ||
| Gene Symbol and Name | Pde6b, phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | CSNB3; PDEB; Pdeb; RP40; nmf137; phosphodiesterase, cGMP, rod receptor, beta polypeptide; r; rd; rd-1; rd1; rd10; retinal degeneration; retinal degeneration 1; retinal degeneration 10; | ||
| Molecular Note | Two mutations have been identified in rd1 mice. A murine leukimia virus (Xmv-28) insertion in reverse orientation in intron 1 is found in all mouse strains with the rd1 phenotype. Further, a nonsense mutation (C to A transversion) in codon 347 that results in a truncation eliminating more than half of the predicted encoded protein, including the catalytic domain has also been identified in all rd1 strains of mice. A specific degradation of mutant transcript during or after pre-mRNA splicing is suggested. [MGI Ref ID J:11513] [MGI Ref ID J:4366] [MGI Ref ID J:51361] | ||
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
C3H Strains
000659 C3H/HeJ 005972 C3H/HeJBirLtJ 001824 C3H/HeJSxJ 000474 C3H/HeSn 000661 C3H/HeSnJ 000658 C3HeB/FeJ 001908 C3HfB/BiJ View C3H Strains (7 strains)
004297 B6.CXB1-Pde6brd10/J 002802 C3.BLiA Pde6b+-Krd/J 001979 C3A.BLiA-Pde6b+.O20-Prph2Rd2/J 001912 C3A.BLiA-Pde6b+/J 003648 C3Sn.BLiA-Pde6b+/Dn 004766 C57BL/6J-Pde6brd1-2J/J 004828 FVB.129P2-Pde6b+ Tyrc-ch/AntJ 004808 STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
Phenotypic Data
Mouse Phenome Database
Festing Inbred Strain Characteristics: C3H
Additional Web Information
C3H strains free of exogenous MMTV
Genetic Quality Control Annual Report
JAX® NOTES, April 1988; 433. H-2 Haplotypes of Mice from Jackson Laboratory Production Colonies.
JAX® NOTES, July 1987; 430. Mammary Tumor Incidence in C3H/HeJ and C3H/OuJ.
JAX® NOTES, May 2000; 480. C3H Strains Free of Exogenous MMTV.
JAX® NOTES, Spring 2002; 485. Genetic Background Effects: Can Your Mice See?
Animal Health Reports
Room Number AX9
Research Applications
This mouse can be used to support research in many areas including:
Pde6brd1 relatedCancer Research
Increased Tumor Incidence (Hepatomas)
Increased Tumor Incidence (Mammary Gland Tumors: late onset)
Research Tools
General Purpose
Sensorineural Research
Retinal Degeneration (Homozygous for Pde6brd1)
Mouse/Human Gene Homologs
retinitis pigmentosa, autosomal recessive
Sensorineural Research
Retinal Degeneration
References
Selected Reference(s)
Additional ReferencesDragani TA; Manenti G; Gariboldi M; Degregorio L; Pierotti MA. 1995. Genetics of liver tumor susceptibility in mice. Toxicol Lett 82-3:613-619. [PubMed: 8597117] [MGI Ref ID J:31816]
Heston WE; Vlahakis G. 1971. Mammary tumors, plaques, and hyperplastic alveolar nodules in various combinations of mouse inbred strains and the different lines of the mammary tumor virus. Int J Cancer 7(1):141-8. [PubMed: 4322934] [MGI Ref ID J:24674]
Outzen HC; Corrow D; Shultz LD. 1985. Attenuation of exogenous murine mammary tumor virus virulence in the C3H/HeJ mouse substrain bearing the Lps mutation. J Natl Cancer Inst 75(5):917-23. [PubMed: 2997536] [MGI Ref ID J:24864]
Price and Supply Information
| Strain Name: | C3H/HeOuJ |
| Stock Number: | 000635 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
USA, Canada and Mexico
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| 3-5 weeks | $19.90 | Female | |||
| 6 weeks | $21.80 | Female | |||
| 7 weeks | $23.70 | Female | |||
| 8 weeks | $25.60 | Female | |||
| 9 weeks | $27.50 | Female | |||
| 10 weeks | $29.40 | Female | |||
| 11 weeks | $31.30 | Female | |||
| 12 weeks | $33.20 | Female | |||
| 3-5 weeks | $19.90 | Male | |||
| 6 weeks | $21.80 | Male | |||
| 7 weeks | $23.70 | Male | |||
| 8 weeks | $25.60 | Male | |||
| 9 weeks | $27.50 | Male | |||
| 10 weeks | $29.40 | Male | |||
| 11 weeks | $31.30 | Male | |||
| 12 weeks | $33.20 | Male | |||
Supply Details
| Standard Supply | Level 2. Up to 100 mice. Larger quantities or custom orders arranged upon request. |
|---|---|
| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-800-422-6423. Shipped at a specific age in weeks. Mice at a precise age in days, littermates and retired breeders are also available. Strains that must be genotyped are not available until five to seven weeks of age. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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