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Strain Name:

A/J

Stock Number:

000646

Availability:

Level 2

Price and Supply Information

General Terms and Conditions

Strain Common Names      A;
Genes & Alleles   Cdh23;   Cdh23ahl;   Dysf;   Dysfprmd;   Hc;   Hc0;

Product Information

Strain Details

Type Inbred Strain
Additional information on Inbred Strains.
Mating SystemSibling x Sibling         (Female x Male)
Specieslaboratory mouse
H2 Haplotypea
GenerationF278 (03-JAN-08)

Appearance
albino
Related Genotype: a/a Tyrp1b/Tyrp1b Tyrc/Tyrc

Important Note
This strain is homozygous for Cdh23ahl, the age related hearing loss 1 mutation, which on this background results in progressive hearing loss with onset between 3 and 5 months of age.

Strain Description
Developed by LC Strong in 1921 from a cross between a Cold Spring Harbor albino and a Bagg albino, the A inbred strain is used widely used in cancer and immunology research. It is highly susceptible to cortisone-induced congenital cleft palate. It has a high incidence of spontaneous lung adenomas, and lung tumors readily develop in response to carcinogens. A high percentage of mammary adenocarcinomas (a large proportion of acinar-type) develop in multiparous females. Rare spontaneous myoepitheliomas arising from myoepithelial cells of various exocrine glands have been observed in The Jackson Laboratory substrains.

A/J mice fed an atherogenic diet (1.25% cholesterol, 0.5% cholic acid, and 15% fat) fail to develop atherosclerotic aortic lesions in contrast to several highly susceptible strains of mice (e.g. C57BL/6J, Stock No. 000664; C57L/J, Stock No. 000668, C57BR/cdJ, Stock No. 000667, and SM/J, Stock No. 000687). In addition to atherosclerosis resistance, A/J mice are resistant to diabetes, obesity, insulin resistance and glucose intolerance. On either chow or high fat diet, A/J mice maintain low glucose and insulin levels.

A/J mice develop cigarette smoke-induced emphysema in approximately half the time when compared with C57BL/6J mice. Structural lung damage caused by induced asthma mimics the phenotype found in asthma patients more closely than does the induced damage in BALB/c mice.

A/J, inbred strain develops a late onset (4-5 months) progressive muscular dystrophy as a result of a homozygous retrotransposon insertion in the dysferlin (Dysf) gene. Myofibers in Dysfprmd homozygotes undergo degeneration and regeneration, and their nuclei are placed nuclei. Proximal muscles are more severely affected than distal muscles (Ho M, et al. 2004). Sequencing of the mitochondrial genome of A/J reveals 10 adenines in a polymorphic adenine repeat sequence in the mt-Tr sequence. This repeat contains 9 adenines in NOD/ShiLtJ, A/HeJ, A/WySnJ, and SKH2/J and 10 adenines in A/J, and NZB/B1NJ, and likely enhances the hearing loss associated with the Cdh23ahl allele (Johnson et al., 2001).

Gene & Allele Details

Allele Symbol Cdh23ahl
Allele Name age related hearing loss 1
Common Name(s) Cdh23753A; mdfw;
Strain of OriginC57BL/6J
Gene Symbol and Name Cdh23, cadherin 23 (otocadherin)
Chromosome 10
Gene Common Name(s) 4930542A03Rik; DFNB12; DKFZp434P2350; FLJ00233; FLJ36499; KIAA1774; KIAA1812; MGC102761; RIKEN cDNA 4930542A03 gene; USH1D; W; age related hearing loss 1; ahl; bob; bobby; bus; bustling; mdfw; modifier of deaf waddler; neuroscience mutagenesis facility, 112; neuroscience mutagenesis facility, 181; neuroscience mutagenesis facility, 252; nmf112; nmf181; nmf252; v; waltzer;
Molecular Note Genetic complementation tests have shown allelism between the mdfw (modifier of deaf waddler) locus and the ahl locus. Further analysis has identified an association between ahl and a G to A transition at nucleotide position 753 of Cdh23. This hypomorphic allele causes in frame skipping of exon 7 and reduced message stability. Twenty-seven strains classified with ahl and carrying the 753A allele include: CD1, RBF/DnJ, PL/J, AKR/J, RF/J, BALB/cBy, A/WySnJ, P/J, SENCARA/PtJ, DBA/1J, ALS/LtJ, C58/J, C57BLKS/J, 129P1/ReJ, C57BR/cd, SKH2/J, BUB/Bn, MA/MyJ, LP/J, 129X1/SvJ, NOR/LtJ, A/J, C57BL/6, NOD/LyJ, DBA/2J, ALR/LtJ, C57L/J. Strains classified with ahl that DO NOT carry this mutation include: C3H/HeSnJ, I/LnJ,YBR/Ei, MRL/MpJ. [MGI Ref ID J:86905]
 
Allele Symbol Dysfprmd
Allele Name progressive muscular dystrophy
Strain of OriginA/J
Gene Symbol and Name Dysf, dysferlin
Chromosome 6
Gene Common Name(s) 2310004N10Rik; AI604795; D6Pas3; DNA segment, Chr 6, Pasteur Institute 3; FER1L1; FLJ00175; FLJ90168; LGMD2B; RIKEN cDNA 2310004N10 gene; expressed sequence AI604795;
Molecular Note A retrotransposon insertion occurred within intron 4, causing aberrant splicing of the gene. Protein was abolished as shown by Northern blot and immunoblot analysis. This allele was found only in A/J mice, not in A/WySnJ, A/HeJ, C57BL/6J, SJL/J, SWR/J or 129/SvJ mice. [MGI Ref ID J:92838]
 
Allele Symbol Hc0
Allele Name deficient
Common Name(s) C5-; C5-d; C5-def; C5-deficient; hco;
Gene Symbol and Name Hc, hemolytic complement
Chromosome 2
Gene Common Name(s) C5; C5a; CPAMD4; FLJ17816; FLJ17822; He; MGC142298; RGD1561905;
General Note

This is an allele characteristic of various inbred mouse strains including the following: A/HeJ, AKR/J, DBA/2J, NZB/B1NJ, SWR/J, B10.D2/oSnJ

Hc was identified as a candidate gene for Abhr2 in a microarray analysis of lung mRNA from A/J, C3H/HeJ, and (A/J x C3H/HeJ)F1 x A/J backcross animals. Hc genotype shows statistically significant correlation to allergen-induced bronchial hyperresponsive phenotype. The A/J allele contains a 2 bp deletion resulting in deficient Hc mRNA and protein production and is associated with susceptibility to allergen-induced bronchial hyperresponsiveness. (J:108211)

Molecular Note A 2 base "TA" deletion at positions 62 and 63 of an 83 base pair exon near the 5' end of the gene is found in the following mouse strains: A/HeJ, AKR/J, DBA/2J, NZB/B1NJ, SWR/J, B10.D2/oSnJ. The consequence of this deletion is the creation of a stop codon starting four bases after the deletion. A truncated product of 216 amino acids is predicted as a result although contradictory reports exist that a larger pro-C5 protein may be synthesized. Nevertheless, macrophages from mouse strains carrying this allele do not secrete complement 5. [MGI Ref ID J:23983]

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

Related Strains

A Strains
000645   A/HeJ
000647   A/WySnJ
View A Strains     (2 strains)

Strains carrying   Cdh23ahl allele
001137   129P1/ReJ
000690   129P3/J
002065   129T2/SvEmsJ
000691   129X1/SvJ
000647   A/WySnJ
003070   ALR/LtJ
003072   ALS/LtJ
004502   B6;AKR-Lxl2/J
001026   BALB/cByJ
000653   BUB/BnJ
005494   C3.129S1(B6)-Grm1rcw/J
000664   C57BL/6J
004764   C57BL/6J-Cdh23v-8J/J
003129   C57BL/6J-Epha4rb-2J/J
004820   C57BL/6J-Kcne12J/J
004703   C57BL/6J-Nmf134/J
004811   C57BL/6J-nmf110/J
004812   C57BL/6J-nmf111/J
004747   C57BL/6J-nmf118/J
004656   C57BL/6J-nmf88/J
004391   C57BL/6J-Chr 13A/J/NaJ
004385   C57BL/6J-Chr 7A/J/NaJ
000662   C57BLKS/J
000667   C57BR/cdJ
000668   C57L/J
000669   C58/J
000657   CE/J
000670   DBA/1J
001140   DBA/1LacJ
000671   DBA/2J
007048   DBA/2J-Gpnmb+/SjJ
002106   KK/HlJ
000675   LG/J
000676   LP/J
000677   MA/MyJ
001976   NOD/ShiLtJ
002050   NOR/LtJ
000679   P/J
002747   SENCARB/PtJ
002335   SKH2/J
003392   STOCK Crb1rd8/J
View Strains carrying   Cdh23ahl     (41 strains)

Strains carrying   Hc0 allele
000645   A/HeJ
000647   A/WySnJ
000648   AKR/J
000460   B10.D2-Hc0 H2d H2-T18c/o2SnJ
000461   B10.D2-Hc0 H2d H2-T18c/oSnJ
000657   CE/J
000671   DBA/2J
007048   DBA/2J-Gpnmb+/SjJ
001800   FVB/NJ
001491   FVB/NMob
000674   I/LnJ
001303   NOD.CB17-Prkdcscid/J
001976   NOD/ShiLtJ
000684   NZB/BlNJ
000682   RF/J
000688   ST/bJ
000689   SWR/J
View Strains carrying   Hc0     (17 strains)

Strains carrying other alleles of Cdh23
008288   B6(Cg)-Cdh23v-11J/J
002756   B6.CAST-Cdh23Ahl+/Kjn
002432   B6J x B6.C-H2bm1/ByJ-Cdh23v-J/J
002552   C57BL/6J-Cdh23v-2J/J
004764   C57BL/6J-Cdh23v-8J/J
004819   C57BL/6J-Cdh23v-9J/J
005016   CByJ;B6-Cdh23v-10J/J
000275   V/LeJ
View Strains carrying other alleles of Cdh23     (8 strains)

Strains carrying other alleles of Dysf
006830   129-Dysftm1Kcam/J
000686   SJL/J
View Strains carrying other alleles of Dysf     (2 strains)

View Strains carrying other alleles of Hc     (5 strains)

Phenotypic Data

Mouse Phenome Database
Mouse Phenome Database / SNP Facility
Festing Inbred Strain Characteristics: A

Additional Web Information

Genetic Quality Control Annual Report
JAX Notes, April 1988; 433. H-2 Haplotypes of Mice from Jackson Laboratory Production Colonies.
JAX Notes, Spring 2004; 493. Chromosome Substitution Strain Panel: A New Tool for Quantitative Trait Loci Analysis.
JAX Notes, Summer 1992; 450. Myoepitheliomas in Inbred Laboratory Mice.
JAX Notes, Winter 2006; 504. JAX® Mice: the Gold Standard Just Got Better.
National Center for Biotechnology Information / SNP Data

Animal Health Reports

Room Number           AX9
Room Number           MP16

Research Applications

This mouse can be used to support research in many areas including:

Cancer Research
Increased Tumor Incidence (Adenomas: lung)
Increased Tumor Incidence (Mammary Gland Tumors: late onset)

Cardiovascular Research
Diet-Induced Atherosclerosis (Relatively Resistant)

Developmental Biology Research
Craniofacial and Palate Defects (congenital cleft palate)
Internal/Organ Defects (gonads)

Internal/Organ Research
Lung Defects (COPD)
Lung Defects (emphysema)

Neurobiology Research
Vestibular and Hearing Defects (Age related hearing loss)

Research Tools
General Purpose
Immunology and Inflammation Research (hybridoma production)

Sensorineural Research
Vestibular and Hearing Defects (Age related hearing loss)

Cdh23ahl related

Neurobiology Research
Vestibular and Hearing Defects (Age related hearing loss)

Sensorineural Research
Vestibular and Hearing Defects (Age related hearing loss)

Dysfprmd related

Mouse/Human Gene Homologs
muscular dystrophy, limb-girdle (type 2B)

Hc0 related

Immunology and Inflammation Research
Immunodeficiency (specific complement deficiency)

Research Tools
Immunology and Inflammation Research (specific complement deficiency) (C5 complement)

References

Selected Reference(s)

Anunciado RV; Nishimura M; Mori M; Ishikawa A; Tanaka S; Horio F; Ohno T; Namikawa T. 2003. Quantitative trait locus analysis of serum insulin, triglyceride, total cholesterol and phospholipid levels in the (SM/J x A/J)F2 mice. Exp Anim 52(1):37-42. [PubMed: 12638235]  [MGI Ref ID J:82274]

Mattson DL. 2001. Comparison of arterial blood pressure in different strains of mice. Am J Hypertens 14(5 Pt 1):405-8. [PubMed: 11368457]  [MGI Ref ID J:109876]

Paigen B; Ishida BY; Verstuyft J; Winters RB; Albee D. 1990. Atherosclerosis susceptibility differences among progenitors of recombinant inbred strains of mice. Arteriosclerosis 10(2):316-23. [PubMed: 2317166]  [MGI Ref ID J:22615]

West DB; Boozer CN; Moody DL; Atkinson RL. 1992. Dietary obesity in nine inbred mouse strains. Am J Physiol 262(6 Pt 2):R1025-32. [PubMed: 1621856]  [MGI Ref ID J:1348]

Additional References

Price and Supply Information

Strain Name: A/J
Stock Number: 000646

Price Details

IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:

*Pricing for Shipping Destination selected:

        International

Price(s) in US dollars ($)Genotype(s) Provided
3 weeks $26.00 Female
4 weeks $26.00 Female
5 weeks $26.40 Female
6 weeks $28.50 Female
7 weeks $31.70 Female
8 weeks $34.30 Female
9 weeks $36.20 Female
10 weeks $38.70 Female
11 weeks $41.20 Female
12 weeks $43.80 Female
13 weeks $46.30 Female
14 weeks $48.70 Female
15 weeks $51.20 Female
3 weeks $25.70 Male
4 weeks $25.70 Male
5 weeks $26.00 Male
6 weeks $28.10 Male
7 weeks $31.20 Male
8 weeks $33.80 Male
9 weeks $35.70 Male
10 weeks $38.40 Male
11 weeks $40.80 Male
12 weeks $43.40 Male
13 weeks $45.90 Male
14 weeks $48.30 Male
15 weeks $50.80 Male

Supply Details

Standard SupplyLevel 2. Up to 100 mice. Larger quantities or custom orders arranged upon request.
Supply Notes Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-207-288-5845.
Shipped at a specific age in weeks. Mice at a precise age in days, littermates and retired breeders are also available.
Strains that must be genotyped are not available until five to seven weeks of age.
This strain is available from some international Charles River Laboratories (CRL) breeding facilities in Japan and/or Europe. For more information, see the Worldwide Distributor List for JAX® Mice.
Genomic DNA is available for this strain from the Mouse DNA Resource.
LicensingSee General Terms and Conditions below  

General Terms and Conditions

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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