Strain Name: |
C57BL/6J |
|---|---|
Stock Number: |
000664 |
Availability: | Level 1 |
Price and Supply Information | |
General Terms and Conditions |
| Strain Common Names | C57 Black; B6; B6J; Black 6; |
| Genes & Alleles | Cdh23; Cdh23ahl; Nnt; NntC57BL/6J; |
Product Information
Strain Details
Type Inbred Strain Additional information on Inbred Strains. Type JAX® GEMM® Strain - Spontaneous Mutation Additional information on JAX® GEMM® Strains. Mating System Sibling x Sibling (Female x Male) Species laboratory mouse H2 Haplotype b Generation F226p (11-JAN-08) Appearance
black
Related Genotype: a/aImportant Note
This strain is homozygous for Cdh23ahl, the age related hearing loss 1 mutation, which on this background results in progressive hearing loss with onset after 10 months of age.Strain Description
C57BL/6 is the most widely used inbred strain. It is commonly used as a general purpose strain and background strain for the generation of congenics carrying both spontaneous and induced mutations. Although this strain is refractory to many tumors, it is a permissive background for maximal expression of most mutations. C57BL/6J mice are used in a wide variety of research areas including cardiovascular biology, developmental biology, diabetes and obesity, genetics, immunology, neurobiology, and sensorineural research. C57BL/6J mice are also commonly used in the production of transgenic mice. Overall, C57BL/6 mice breed well, are long-lived, and have a low susceptibility to tumors. Primitive hematopoietic stem cells from C57BL/6J mice show greatly delayed senescence relative to BALB/c and DBA/2J. This is a dominant trait. Other characteristics include: 1) a high susceptibility to diet-induced obesity, type 2 diabetes, and atherosclerosis; 2) a high incidence of microphthalmia and other associated eye abnormalities; 3) resistance to audiogenic seizures; 4) low bone density; 5) hereditary hydrocephalus (early reports indicate 1 - 4 %); 6) hairloss associated with overgrooming, 7) a preference for alcohol and morphine; 8) late-onset hearing loss; and 9) increased incidence of hydrocephalus and malocclusion.C57BL/6J mice fed a high-fat diet develop obesity, mild to moderate hyperglycemia, and hyperinsulinemia (see JAX ® DIO Service and JAX ® Inventoried DIO Mice). C57BL/6J mice fed an atherogenic diet (1.25% cholesterol, 0.5% cholic acid and 15% fat) for 14 weeks develop lesions in the range of 4500 to 8000 um2 atherosclerotic aortic lesions/aortic cross-section. The variation in aortic lesions found among various inbred strains has led to the identification of the existence of eight genes affecting atherosclerosis, Ath1 to Ath8. C57BL/6J mice also develop severe and progressive hearing loss later in life. Histopathological changes associated with age-related hearing loss include the disruption of both outer and inner hair cells. C57BL/6 mice are also more susceptible to noise-induced hearing loss. Age related hearing loss 1 (Ahl), a major gene responsible for this hearing loss, was mapped in an intersubspecific backcross by measuring elevated auditory-evoked brainstem response (ABR) thresholds. Ahl is located on Chromosome 10 near marker D10Mit5. A naturally occurring deletion in nicotinamide nucleotide transhydrogenase (Nnt) exons 7-11 occurred in C57BL/6J sometime prior to 1984. This deletion results in the absence of the NNT protein, and is associated with impaired glucose homeostasis control and reduced insulin secretion. This mutation is not found in C57BL/6JEi, C57BL/6N, C57BL/6ByJ, C57BL/10J, C57L/J, or C58/J (Toye AA, et al, Diabetologia, 2005).
C57BL/6J was the DNA source for the international collaboration that generated the first high quality draft sequence of the mouse genome. 5 SNP differences have been identified that distinguish C57BL/6J from C57BL/6ByJ and C57BL/6NJ. Both C57BL/6ByJ and C57BL/6NJ type as follows: 08-015199792-M is C; 11-004367508-M is A; 13-041017317-M is C; 15-057561875-M is G; 19-049914266-M is T. C57BL/6J types as follows: 08-015199792-M is T; 11-004367508-M is G; 13-041017317-M is T; 15-057561875-M is A; 19-049914266-M is G (Petkov and Wiles, 2005.)
Strain Development
The C57BL/6J inbred strain was created by Dr. CC Little from the mating of female 57 with male 52 from Miss Abbie Lathrop's stock. The same cross gave rise to the C57L and C57BR strains.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Cdh23ahl | ||
|---|---|---|---|
| Allele Name | age related hearing loss 1 | ||
| Common Name(s) | Cdh23753A; mdfw; | ||
| Strain of Origin | C57BL/6J | ||
| Gene Symbol and Name | Cdh23, cadherin 23 (otocadherin) | ||
| Chromosome | 10 | ||
| Gene Common Name(s) | 4930542A03Rik; DFNB12; DKFZp434P2350; FLJ00233; FLJ36499; KIAA1774; KIAA1812; MGC102761; RIKEN cDNA 4930542A03 gene; USH1D; W; age related hearing loss 1; ahl; bob; bobby; bus; bustling; mdfw; modifier of deaf waddler; neuroscience mutagenesis facility, 112; neuroscience mutagenesis facility, 181; neuroscience mutagenesis facility, 252; nmf112; nmf181; nmf252; v; waltzer; | ||
| Molecular Note | Genetic complementation tests have shown allelism between the mdfw (modifier of deaf waddler) locus and the ahl locus. Further analysis has identified an association between ahl and a G to A transition at nucleotide position 753 of Cdh23. This hypomorphic allele causes in frame skipping of exon 7 and reduced message stability. Twenty-seven strains classified with ahl and carrying the 753A allele include: CD1, RBF/DnJ, PL/J, AKR/J, RF/J, BALB/cBy, A/WySnJ, P/J, SENCARA/PtJ, DBA/1J, ALS/LtJ, C58/J, C57BLKS/J, 129/ReJ, C57BR/cd, SKH2/J, BUB/Bn, MA/MyJ, LP/J, 129/ScJ, NOR/LtJ, A/J, C57BL/6, NOD/LyJ, DBA/2J, ALR/LtJ, C57L/J. Strains classified with ahl that DO NOT carry this mutation include: C3H/HeSnJ, I/LnJ,YBR/Ei, MRL/MpJ. [J:86905] | ||
| Allele Symbol | NntC57BL/6J | ||
| Allele Name | C57BL/6J | ||
| Strain of Origin | C57BL/6J | ||
| Gene Symbol and Name | Nnt, nicotinamide nucleotide transhydrogenase | ||
| Chromosome | 13 | ||
| Gene Common Name(s) | 4930423F13Rik; AI323702; BB168308; MGC109126; MGC126502; MGC126503; RIKEN cDNA 4930423F13 gene; expressed sequence AI323702; expressed sequence BB168308; | ||
| Molecular Note | This allele contains a stretch of 17,814 bp missing between exons 6 and 12. RT-PCR demonstrated cDNA corresponding to exons 7-11 was absent. Mature protein was not detected in these mutants. [J:108213] | ||
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
C57BL Strains
000665 C57BL/10J 003752 C57BL/10ScNJ 000476 C57BL/10ScSnJ 000666 C57BL/10SnJ 001822 C57BL/10SxJ 001197 C57BL/10WtRkJ 000663 C57BL/6By 001139 C57BL/6ByJ 000924 C57BL/6JEiJ 005304 C57BL/6NJ View C57BL Strains (10 strains)
001137 129P1/ReJ 000690 129P3/J 002065 129T2/SvEmsJ 000691 129X1/SvJ 000646 A/J 000647 A/WySnJ 003070 ALR/LtJ 003072 ALS/LtJ 004502 B6;AKR-Lxl2/J 001026 BALB/cByJ 000653 BUB/BnJ 005494 C3.129S1(B6)-Grm1rcw/J 004764 C57BL/6J-Cdh23v-8J/J 003129 C57BL/6J-Epha4rb-2J/J 004820 C57BL/6J-Kcne12J/J 004703 C57BL/6J-Nmf134/J 004811 C57BL/6J-nmf110/J 004812 C57BL/6J-nmf111/J 004747 C57BL/6J-nmf118/J 004656 C57BL/6J-nmf88/J 004391 C57BL/6J-Chr 13A/J/NaJ 004385 C57BL/6J-Chr 7A/J/NaJ 000662 C57BLKS/J 000667 C57BR/cdJ 000668 C57L/J 000669 C58/J 000657 CE/J 000670 DBA/1J 001140 DBA/1LacJ 000671 DBA/2J 007048 DBA/2J-Gpnmb+/SjJ 002106 KK/HlJ 000675 LG/J 000676 LP/J 000677 MA/MyJ 001976 NOD/ShiLtJ 002050 NOR/LtJ 000679 P/J 002747 SENCARB/PtJ 002335 SKH2/J 003392 STOCK Crb1rd8/J
002756 B6.CAST-Cdh23Ahl+/Kjn 002432 B6J x B6.C-H2bm1/ByJ-Cdh23v-J/J 002552 C57BL/6J-Cdh23v-2J/J 004764 C57BL/6J-Cdh23v-8J/J 004819 C57BL/6J-Cdh23v-9J/J 005016 CByJ;B6-Cdh23v-10J/J 000275 V/LeJ
Phenotypic Data
Body Weight Information - JAX® Mice Strain C57BL/6J (000664)Mouse Phenome Database
(This chart reflects the typical correlation between body weight and age for mice maintained in production colonies at The Jackson Laboratory.)
Mouse Phenome Database - atherogenic diet
Mouse Phenome Database - body weight
Mouse Phenome Database - bone mineral density and content
Mouse Phenome Database - cardiovascular
Mouse Phenome Database - cholesterol
Mouse Phenome Database - disease susceptibility
Mouse Phenome Database - ethanol effects
Mouse Phenome Database - food and water intake
Mouse Phenome Database - glucose
Mouse Phenome Database - hearing
Mouse Phenome Database - hematology
Mouse Phenome Database / SNP Facility
Festing Inbred Strain Characteristics: C57BL
NEW -- JAX® Physiological Data Summary
NEW -- JAX® Physiological Data Protocol
Additional Web Information
3D MRI Digital Atlas Database of Adult C57BL/6J Mouse Brain
NEW -- JAX® Physiological Data Protocol
NEW -- JAX® Physiological Data Summary
Genetic Quality Control Annual Report
JAX Notes, April 1988; 433. H-2 Haplotypes of Mice from Jackson Laboratory Production Colonies.
JAX Notes, Fall 1995; 463. Inbred C57 Black Mice: Microphthalmia and Ocular Infections.
JAX Notes, Fall 2003; 491. JAX West Expansion
JAX Notes, Fall 2003; 491. The Importance of Understanding Substrains in the Genomic Age.
JAX Notes, Fall 2006; 503. Cause of Glucose Intolerance in C57BL/6J Mice Discovered.
JAX Notes, January 1988; 432. Arthritis Models in the Mouse.
JAX Notes, July 1989; 438. Profile: C57BL/6J.
JAX Notes, October 1987; 431. Alopecia (loss of hair) in C57BL/6J and Related Strains.
JAX Notes, October 1989; 439. Splenic Melanosis in Black Mice.
JAX Notes, Spring 1990; 441. Imperforate Vagina and Mucometra in Mice.
JAX Notes, Spring 2003; 489. Malocclusion in the Laboratory Mouse.
JAX Notes, Spring 2003; 489. Mouse Genome Sequence Released.
JAX Notes, Spring 2004; 493. Chromosome Substitution Strain Panel: A New Tool for Quantitative Trait Loci Analysis.
JAX Notes, Spring 2007; 505. C57BL/6J Strain Used to Construct the Allen Brain Atlas.
JAX Notes, Summer 1994; 458. Ly5 Gene Nomenclature, C57BL/6J and SJL/J - A History of Change.
JAX Notes, Summer 2003; 490. Charles River Japan Now Breeding JAX® Mice Strain C57BL/6J.
JAX Notes, Summer 2003; 490. Hydrocephalus in Laboratory Mice.
JAX Notes, Summer 2006; 502. Characterization of DIO Model is Refined.
JAX Notes, Winter 2002; 488. Colony Expansions Completed for JAX® Mice Strains C57BL/6J and BALB/cJ.
JAX Notes, Winter 2006; 504. JAX® Mice: the Gold Standard Just Got Better.
National Center for Biotechnology Information / SNP Data
Animal Health Reports
Room Number AX1
Room Number AX10
Room Number AX3
Room Number AX4
Room Number AX6
Room Number MP14
Room Number MP15
Room Number WR4
Research Applications
This mouse can be used to support research in many areas including:
Cdh23ahl relatedCardiovascular Research
Diet-Induced Atherosclerosis (Susceptible)
Developmental Biology Research
Eye Defects
Lymphoid Tissue Defects (hematopoietic defects)
Skeletal Defects
Diabetes and Obesity Research
Hyperglycemia (diet-induced, moderate)
Hyperinsulinemia (diet-induced)
Insulin Resistance (diet-induced)
Obesity With Diabetes (diet-induced, moderate)
Type 2 Diabetes (NIDDM) (diet-induced)
Hematological Research
Hematopoietic Defects
Immunology and Inflammation Research
Lymphoid Tissue Defects (hematopoietic development)
Neurobiology Research
Behavioral and Learning Defects
Vestibular and Hearing Defects (Age related hearing loss)
Research Tools
General Purpose
Genetics Research (Mutagenesis and Transgenesis: Production of Transgenic Mice)
Hematological Research
Immunology and Inflammation Research (background strain for histocompatibility congenics)
Sensorineural Research
Eye Defects
Vestibular and Hearing Defects (Age related hearing loss)
Neurobiology Research
Vestibular and Hearing Defects (Age related hearing loss)
Sensorineural Research
Vestibular and Hearing Defects (Age related hearing loss)
References
Selected Reference(s)
Additional ReferencesBlack BL; Croom J; Eisen EJ; Petro AE; Edwards CL; Surwit RS. 1998. Differential effects of fat and sucrose on body composition in A/J and C57BL/6 mice. Metabolism 47(11):1354-9. [PubMed: 9826212] [J:50965]
Drake TA; Schadt E; Hannani K; Kabo JM; Krass K; Colinayo V; Greaser LE rd; Goldin J; Lusis AJ. 2001. Genetic loci determining bone density in mice with diet-induced atherosclerosis. Physiol Genomics 5(4):205-15. [PubMed: 11328966] [J:69682]
Ishida BY; Blanche PJ; Nichols AV; Yashar M; Paigen B. 1991. Effects of atherogenic diet consumption on lipoproteins in mouse strains C57BL/6 and C3H. J Lipid Res 32(4):559-68. [PubMed: 1856605] [J:109926]
Jiao S; Cole TG; Kitchens RT; Pfleger B; Schonfeld G. 1990. Genetic heterogeneity of plasma lipoproteins in the mouse: control of low density lipoprotein particle sizes by genetic factors. J Lipid Res 31(3):467-77. [PubMed: 1971301] [J:15484]
Kirk EA; Moe GL; Caldwell MT; Lernmark JA; Wilson DL; LeBoeuf RC. 1995. Hyper- and hypo-responsiveness to dietary fat and cholesterol among inbred mice: searching for level and variability genes. J Lipid Res 36(7):1522-32. [PubMed: 7595076] [J:28648]
Nishina PM; Verstuyft J; Paigen B. 1990. Synthetic low and high fat diets for the study of atherosclerosis in the mouse. J Lipid Res 31(5):859-69. [PubMed: 2380634] [J:27046]
Nishina PM; Wang J; Toyofuku W; Kuypers FA; Ishida BY; Paigen B. 1993. Atherosclerosis and plasma and liver lipids in nine inbred strains of mice. Lipids 28(7):599-605. [PubMed: 8355588] [J:13267]
Paigen B. 1995. Genetics of responsiveness to high-fat and high- cholesterol diets in the mouse. Am J Clin Nutr 62(2):458S-462S. [PubMed: 7625360] [J:28248]
Paigen B; Ishida BY; Verstuyft J; Winters RB; Albee D. 1990. Atherosclerosis susceptibility differences among progenitors of recombinant inbred strains of mice. Arteriosclerosis 10(2):316-23. [PubMed: 2317166] [J:22615]
Paigen B; Morrow A; Brandon C; Mitchell D; Holmes P. 1985. Variation in susceptibility to atherosclerosis among inbred strains of mice. Atherosclerosis 57(1):65-73. [PubMed: 3841001] [J:109950]
Toye AA; Lippiat JD; Proks P; Shimomura K; Bentley L; Hugill A; Mijat V; Goldsworthy M; Moir L; Haynes A; Quarterman J; Freeman HC; Ashcroft FM; Cox RD. 2005. A genetic and physiological study of impaired glucose homeostasis control in C57BL/6J mice. Diabetologia 48(4):675-86. [PubMed: 15729571] [J:106692]
Waterston RH; Lindblad-Toh K; Birney E; Rogers J; Abril JF; Agarwal P; Agarwala R; Ainscough R; Alexandersson M; An P; Antonarakis SE; Attwood J; Baertsch R; Bailey J; Barlow K; Beck S; Berry E; Birren B; Bloom T; Bork P; Botcherby M; Bray N; Brent MR; Brown DG; Brown SD; Bult C; Burton J; Butler J; Campbell RD; Carninci P; Cawley S; Chiaromonte F; Chinwalla AT; Church DM; Clamp M; Clee C; Collins FS; Cook LL; Copley RR; Coulson A; Couronne O; Cuff J; Curwen V; Cutts T; Daly M; David R; Davies J; Delehaun. 2002. Initial sequencing and comparative analysis of the mouse genome. Nature 420(6915):520-62. [PubMed: 12466850] [J:80507]
Price and Supply Information
| Strain Name: | C57BL/6J |
| Stock Number: | 000664 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
USA, Canada and Mexico
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Base Price (3-5 weeks) | $12.95 | Male | |||
| Base Price (3-5 weeks) | $14.35 | Female | |||
| 6 weeks | $14.80 | Male | |||
| 6 weeks | $16.20 | Female | |||
| 7 weeks | $16.65 | Male | |||
| 7 weeks | $18.05 | Female | |||
| 8 weeks | $18.50 | Male | |||
| 8 weeks | $19.90 | Female | |||
Supply Details
| Standard Supply | Level 1. JAX Ready Strain® (readily available). |
|---|---|
| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-800-422-6423. Shipped at a specific age in weeks. Mice at a precise age in days, littermates and retired breeders are also available. Strains that must be genotyped are not available until five to seven weeks of age. This strain is available from both the Bar Harbor and California facilities. This strain is available from some international Charles River Laboratories (CRL) breeding facilities in Japan and/or Europe. For more information, see the Worldwide Distributor List for JAX® Mice. Timed pregnant females are available from our Bar Harbor and California facilities. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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