Strain Name:

SM/J

Stock Number:

000687

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Availability:

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Common Names: small;    
SM/J mice carry a number of rare polymorphic alleles and are often matched to other strains for quantitative trait locus analysis. These mice are susceptible to diet-induced obesity and diet-induced atherosclerosis. SM/J mice exhibit a hyperresponsiveness to B cell mitogens. Small in size at birth and through weaning, SM/J mice attain a normal body weight as they age.

Description

Strain Information

Type Inbred Strain;
Additional information on Inbred Strains.
Visit our online Nomenclature tutorial.
Mating SystemSibling x Sibling         (Female x Male)   01-MAR-06
Breeding Considerations This strain is a challenging breeder.
Specieslaboratory mouse
H2 Haplotypev
GenerationF215 (20-JAN-14)
Generation Definitions

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Appearance
white-bellied agouti
Related Genotype: Aw/a

black
Related Genotype: a/a

Description
SM/J mice carry a number of rare polymorphic alleles and are often matched to LG/J (Stock No. 000675), A/J (Stock No. 000646) or NZB/BINJ (Stock No. 000684) for quantitative trait locus analysis. These mice are susceptible to diet-induced obesity and diet-induced atherosclerosis. SM/J mice exhibit a hyperresponsiveness to B cell mitogens (Clark et al. 1981, Engel et al. 1981). A point mutation in Neu1 is responsible for a partial deficiency of lysosomal neuraminadase and may explain the altered immune response (Rottier et al. 1998). Small in size at birth and through weaning, SM/J mice attain a normal body weight as they age.

Development
SM/J, created by MacArthur in 1939 from crosses involving 7 inbred strains - among them DBA - followed by inbreeding with selection for small body size, is segregating white-bellied agouti (Aw) vs. nonagouti (a) at the agouti locus.

Related Strains

Strains carrying   Il3ram1 allele
000645   A/HeJ
000646   A/J
000647   A/WySnJ
000648   AKR/J
000653   BUB/BnJ
000669   C58/J
000657   CE/J
000684   NZB/BlNJ
000682   RF/J
View Strains carrying   Il3ram1     (9 strains)

Additional Web Information

JAX® NOTES, April 1988; 433. H-2 Haplotypes of Mice from Jackson Laboratory Production Colonies.

Phenotype

Phenotype Information

View Phenotypic Data

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).
Neuraminidase Deficiency
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Il3ram1/Il3ram1

        SM/J
  • hematopoietic system phenotype
  • abnormal common myeloid progenitor cell morphology
    • CFU-GM assays using bone marrow derived cells yield very few colonies in repsonse to interleukin 3   (MGI Ref ID J:24918)

Neu1a/Neu1a

        SM/J
  • homeostasis/metabolism phenotype
  • abnormal enzyme/coenzyme activity
    • deficient enzymatic activity of neuraminidase was reported   (MGI Ref ID J:43930)
    • tissue specific decrease in sialidase activity   (MGI Ref ID J:147881)
  • growth/size/body phenotype
  • decreased body weight
    • compared to C57BL/6 controls   (MGI Ref ID J:147881)
  • liver/biliary system phenotype
  • abnormal Kupffer cell morphology
    • cells contain large membrane bound bodies containing lipid droplets and membrane like structures that are not present in C57BL/6 controls   (MGI Ref ID J:147881)
  • renal/urinary system phenotype
  • abnormal proximal convoluted tubule morphology
    • some epithelial cells accumulate large membrane-bound structures containing membranous whorls   (MGI Ref ID J:147881)
    • cells with high levels of membranous accumulations degenerate releasing cellular debris into the intercellular space and the lumen of the tubules   (MGI Ref ID J:147881)
  • hematopoietic system phenotype
  • abnormal Kupffer cell morphology
    • cells contain large membrane bound bodies containing lipid droplets and membrane like structures that are not present in C57BL/6 controls   (MGI Ref ID J:147881)
  • immune system phenotype
  • abnormal Kupffer cell morphology
    • cells contain large membrane bound bodies containing lipid droplets and membrane like structures that are not present in C57BL/6 controls   (MGI Ref ID J:147881)
  • cardiovascular system phenotype
  • abnormal Kupffer cell morphology
    • cells contain large membrane bound bodies containing lipid droplets and membrane like structures that are not present in C57BL/6 controls   (MGI Ref ID J:147881)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cardiovascular Research
Diet-Induced Atherosclerosis
      Susceptible

Developmental Biology Research
Growth Defects

Diabetes and Obesity Research
Obesity Without Diabetes
      diet-induced

Immunology, Inflammation and Autoimmunity Research
Immunodeficiency
      defects in humoral immune responses

Il3ram1 related

Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
      genes regulating susceptibility to infectious disease and endotoxin

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Il3ram1
Allele Name mutation 1
Allele Type Spontaneous
Common Name(s) Il3raA/J; Il3ran;
Strain of OriginA/J
Gene Symbol and Name Il3ra, interleukin 3 receptor, alpha chain
Chromosome 14
Gene Common Name(s) CD123; Cyrl; IL-3 receptor alpha chain; IL3R; IL3RAY; IL3RX; IL3RY; SUT-1; hIL-3Ra;
Molecular Note Sequence analysis revealed A/J mice lack the sequence corresponding to exon 8, which encodes 10 amino acid residues in the extracellular domain. Aberrant splicing was due to a 5 base pair deletion at the branch point in intron 7. [MGI Ref ID J:23971]
 
Allele Symbol Neu1a
Allele Name a variant
Allele Type Not Applicable
Common Name(s) Neu-1s;
Strain of OriginSM/J
Gene Symbol and Name Neu1, neuraminidase 1
Chromosome 17
Gene Common Name(s) AA407268; AA407316; Aglp; Apl; Bat-7; Bat7; G9; HLA-B-associated transcript 7; Map-2; NANH; NEU; Neu-1; SIAL1; acid phosphatase, liver; alpha glucosidase processing; expressed sequence AA407268; expressed sequence AA407316; lysosomal sialidase; mannosidase processing 2; sialidase 1;
General Note Low activity determined by the Neu1a allele occurs in the SM/J inbred strain and in wild mice in the area of Ann Arbor, Michigan; all other inbred strains have high activity determined by the Neu1b allele. Heterozygotes have intermediate activity. Neuraminidase removes extra sialic acid residues from these enzymes. The defective neuraminidase of Neu1a> mice, by failing to remove the extra sialic acid, changes their electrophoretic mobility (J:6480). Level of activity of neuraminidase in activated T lymphocytes is also depressed in Neu1a/Neu1a mice (J:7976).
Molecular Note Sequencing of the a allele revealed 5 nucleotide differences compared to the b allele. These changes alter the amino acid residues 11, 15, 17, 19 and 209 of the encoded protein from Gly, Tyr, Ala, Arg and Leu to Arg, Cys, Val, Cys and Ile, respectively. This encoded protein has 85-95% less activity than the protein encoded by the b allele as demonstrated in an in vitro assay. Further studies attributed most of the activity loss to the variation at position 209. Later studies detected 4 silent mutations in the signal peptide sequence and 2 mutations (c.-240C>T and c.-519G>A) in the promoter region. The mutation c.-519G>A creates a novel binding site for Nkx3-1 and Nkx3-2. In vitro assays demonstrated that binding of Nkx3-2 specifically represses promoter driven expression. [MGI Ref ID J:147881] [MGI Ref ID J:43930] [MGI Ref ID J:77236]

Genotyping

Genotyping Information

Inbred mouse strains are maintained through sibling (sister x brother) matings; no genotyping required.

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Anunciado RV; Nishimura M; Mori M; Ishikawa A; Tanaka S; Horio F; Ohno T; Namikawa T. 2001. Quantitative trait loci for body weight in the intercross between SM/J and A/J mice. Exp Anim 50(4):319-24. [PubMed: 11515095]  [MGI Ref ID J:71465]

Ehrich TH; Kenney JP; Vaughn TT; Pletscher LS; Cheverud JM. 2003. Diet, obesity, and hyperglycemia in LG/J and SM/J mice. Obes Res 11(11):1400-10. [PubMed: 14627762]  [MGI Ref ID J:86873]

Korstanje R; Albers JJ; Wolfbauer G; Li R; Tu AY; Churchill GA; Paigen BJ. 2004. Quantitative trait locus mapping of genes that regulate phospholipid transfer activity in SM/J and NZB/BlNJ inbred mice. Arterioscler Thromb Vasc Biol 24(1):155-60. [PubMed: 14592843]  [MGI Ref ID J:86340]

Korstanje R; Li R; Howard T; Kelmenson P; Marshall J; Paigen B; Churchill G. 2004. Influence of sex and diet on quantitative trait loci for HDL cholesterol levels in an SM/J by NZB/BlNJ intercross population. J Lipid Res 45(5):881-8. [PubMed: 14993241]  [MGI Ref ID J:89309]

Macarthur JW. 1949. Selection for Small and Large Body Size in the House Mouse. Genetics 34(2):194-209. [PubMed: 17247310]  [MGI Ref ID J:2452]

Macarthur JW. 1944. Genetics of body size and related characters Am Naturalist 78:142-57.  [MGI Ref ID J:25351]

Paigen B; Ishida BY; Verstuyft J; Winters RB; Albee D. 1990. Atherosclerosis susceptibility differences among progenitors of recombinant inbred strains of mice. Arteriosclerosis 10(2):316-23. [PubMed: 2317166]  [MGI Ref ID J:22615]

Petkov PM; Cassell MA; Sargent EE; Donnelly CJ; Robinson P; Crew V; Asquith S; Haar RV; Wiles MV. 2004. Development of a SNP genotyping panel for genetic monitoring of the laboratory mouse. Genomics 83(5):902-11. [PubMed: 15081119]  [MGI Ref ID J:89298]

Pitman WA; Korstanje R; Churchill GA; Nicodeme E; Albers JJ; Cheung MC; Staton MA; Sampson SS; Harris S; Paigen B. 2002. Quantitative trait locus mapping of genes that regulate HDL cholesterol in SM/J and NZB/B1NJ inbred mice. Physiol Genomics 9(2):93-102. [PubMed: 12006675]  [MGI Ref ID J:76707]

Additional References

Anunciado RV; Nishimura M; Mori M; Ishikawa A; Tanaka S; Horio F; Ohno T; Namikawa T. 2003. Quantitative trait locus analysis of serum insulin, triglyceride, total cholesterol and phospholipid levels in the (SM/J x A/J)F2 mice. Exp Anim 52(1):37-42. [PubMed: 12638235]  [MGI Ref ID J:82274]

Frankel WN; Lee BK; Stoye JP; Coffin JM; Eicher EM. 1992. Characterization of the endogenous nonecotropic murine leukemia viruses of NZB/B1NJ and SM/J inbred strains. Mamm Genome 2(2):110-22. [PubMed: 1311971]  [MGI Ref ID J:1883]

Hara T; Ichihara M; Takagi M; Miyajima A. 1995. Interleukin-3 (IL-3) poor-responsive inbred mouse strains carry the identical deletion of a branch point in the IL-3 receptor alpha subunit gene. Blood 85(9):2331-6. [PubMed: 7727767]  [MGI Ref ID J:24918]

Klingenberg CP; Leamy LJ; Cheverud JM. 2004. Integration and modularity of quantitative trait locus effects on geometric shape in the mouse mandible. Genetics 166(4):1909-21. [PubMed: 15126408]  [MGI Ref ID J:89613]

Rottier RJ; Bonten E; d'Azzo A. 1998. A point mutation in the neu-1 locus causes the neuraminidase defect in the SM/J mouse. Hum Mol Genet 7(2):313-21. [PubMed: 9425240]  [MGI Ref ID J:77236]

Routman EJ; Cheverud JM. 1995. Polymorphism for PCR-analyzed microsatellites between the inbred mouse strains LG and SM. Mamm Genome 6(6):401-4. [PubMed: 7647461]  [MGI Ref ID J:26136]

Il3ram1 related

Gainsford T; Roberts AW; Kimura S; Metcalf D; Dranoff G; Mulligan RC; Begley CG; Robb L; Alexander WS. 1998. Cytokine production and function in c-mpl-deficient mice: no physiologic role for interleukin-3 in residual megakaryocyte and platelet production. Blood 91(8):2745-52. [PubMed: 9531584]  [MGI Ref ID J:47462]

Hara T; Ichihara M; Takagi M; Miyajima A. 1995. Interleukin-3 (IL-3) poor-responsive inbred mouse strains carry the identical deletion of a branch point in the IL-3 receptor alpha subunit gene. Blood 85(9):2331-6. [PubMed: 7727767]  [MGI Ref ID J:24918]

Ichihara M; Hara T; Takagi M; Cho LC; Gorman DM; Miyajima A. 1995. Impaired interleukin-3 (IL-3) response of the A/J mouse is caused by a branch point deletion in the IL-3 receptor alpha subunit gene. EMBO J 14(5):939-50. [PubMed: 7889941]  [MGI Ref ID J:23971]

Neu1a related

Carrillo MB; Milner CM; Ball ST; Snoek M; Campbell RD. 1997. Cloning and characterization of a sialidase from the murine histocompatibility-2 complex: low levels of mRNA and a single amino acid mutation are responsible for reduced sialidase activity in mice carrying the Neu1a allele. Glycobiology 7(7):975-86. [PubMed: 9363440]  [MGI Ref ID J:43930]

Champigny MJ; Mitchell M; Fox-Robichaud A; Trigatti BL; Igdoura SA. 2009. A point mutation in the neu1 promoter recruits an ectopic repressor, Nkx3.2 and results in a mouse model of sialidase deficiency. Mol Genet Metab 97(1):43-52. [PubMed: 19217813]  [MGI Ref ID J:147881]

Hildebrand CE; Gonzalez FJ; Kozak CA; Nebert DW. 1985. Regional linkage analysis of the dioxin-inducible P-450 gene family on mouse chromosome 9. Biochem Biophys Res Commun 130(1):396-406. [PubMed: 4040754]  [MGI Ref ID J:7969]

Kijimoto-Ochiai S; Koda T; Suwama T; Matsukawa H; Fujii M; Tomobe K; Nishimura M. 2008. Low expression of Neu2 sialidase in the thymus of SM/J mice-existence of neuraminidase positive cells 'Neu-medullocyte' in the murine thymus. Glycoconj J 25(8):787-96. [PubMed: 18553168]  [MGI Ref ID J:153934]

Klein D; Klein J. 1982. Polymorphism of the Apl (Neu-1) locus in the mouse. Immunogenetics 16(2):181-4. [PubMed: 7141491]  [MGI Ref ID J:6900]

Potier M; Lu Shun Yan D; Womack JE. 1979. Neuraminidase deficiency in the mouse. FEBS Lett 108(2):345-8. [PubMed: 520573]  [MGI Ref ID J:123679]

Rottier RJ; Bonten E; d'Azzo A. 1998. A point mutation in the neu-1 locus causes the neuraminidase defect in the SM/J mouse. Hum Mol Genet 7(2):313-21. [PubMed: 9425240]  [MGI Ref ID J:77236]

Womack JE; David CS. 1982. Mouse gene for neuraminidase activity (Neu-1) maps to the D end of H-2. Immunogenetics 16(2):177-80. [PubMed: 7141490]  [MGI Ref ID J:6899]

Womack JE; Yan DL; Potier M. 1981. Gene for neuraminidase activity on mouse chromosome 17 near h-2: pleiotropic effects on multiple hydrolases. Science 212(4490):63-5. [PubMed: 7209520]  [MGI Ref ID J:6480]

Yang A; Gyulay G; Mitchell M; White E; Trigatti BL; Igdoura SA. 2012. Hypomorphic sialidase expression decreases serum cholesterol by downregulation of VLDL production in mice. J Lipid Res 53(12):2573-85. [PubMed: 22984145]  [MGI Ref ID J:190648]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Mating SystemSibling x Sibling         (Female x Male)   01-MAR-06
Breeding Considerations This strain is a challenging breeder.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


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Price per mouse (US dollars $)Gender
Individual Mouse $135.00Female or Male  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)Gender
Individual Mouse $175.50Female or Male  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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